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All Journal Dental Journal (Majalah Kedokteran Gigi) Biomolecular and Health Science Journal Conservative Dentistry Journal Indonesian Journal of Dental Medicine
Dian Agustin Wahjuningrum
Faculty Of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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The Difference of Irrigant Solution between Red Pine Extract (Pinus densiflora) and Green Pine Extract (Pinus merkusii) Against Cleanliness of Root Canal Walls Dian Agustin Wahjuningrum; Setyabudi Setyabudi; Sukaton Sukaton; Yuline Krishartini
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.62-65

Abstract

Background: Smear layer is an accumulation of organic material, debris, and inorganic material in root canal wall. Irrigant solution must be able to eliminate organic and inorganic materials. Herbal extract such as pine contain saponins that can dissolve organic and inorganic materials. Korea is a country that has used pine in this case red pine as an antibacterial medicament. In Indonesia, there are also green pine species. Purpose: To explain the differences in cleanliness of the root canals after irrigation using extracts of red pine (Pinus densiflora) and green pine (Pinus merkusii). Methods: This study used twenty-seven mandibular premolar teeth which were divided into 3 groups each group consisted of 9 premolar teeth. All samples were prepared using ProTaper for Hand Use and irrigated. Group I was irrigated using 2.5% NaOCl, group II was irrigated with red pine extract, and group III was irrigated using green pine extract. Tooth that has been prepared, were cut vertically into 2 and cut horizontally in 1/3 apical section. The root canal surface was observed using a scanning electron microscope. Photomicrograph was observed by three observers using a score and performed an analysis test. Results: There were no difference between red pine (Pinus densiflora) and green pine (Pinus merkusii) in root canal cleanliness with a significance value of 0.169 (p> 0.05) Conclusion: Red pine extract had the same smear layer cleaning power as green pine extract.
One visit endodontic on asymtomatic mummificated mandibular molar Sholeh Ardjanggi; Dian Agustin Wahjuningrum; Tamara Yuanita; Eric Priyo Prasetyo
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.80-82

Abstract

Background. The main goal of root canal treatment is the prevention or treatment of apical periodontitis, which leads to the preservation of natural teeth. Traditionally, root canal treatment is carried out in a number of visits, using extra disinfecting agents in addition to irrigant used during cleaning and shaping procedures that primarily aim to reduce or eliminate microorganisms and their byproducts. from the root canal system before it is obtained. Recent advances in Endodontic technology, attracting dental experts and endodontists to perform root canal treatment in one visit. Numerous studies evaluate the effectiveness of single-versus multiple-appointment root canal treatment have been published, which reported no significant differences in effectiveness (healing rates) between these two treatment regimens.Purpose : preservation of the integrity of the molar teeth that have symptomatic complaints by taking proper care with a short visit time. Caset: A 21-year-old woman presents with concern in her lower left tooth, the patient tells that the tooth had been treated before at the dentist when she was a child. On clinical examination there is a composite restoration in occlusal tooth # 36, non vital teeth. On periapical radiology there are radiopaque on pulp chamber and a diffuse radiolucent area in the apical area of tooth # 36, then the diagnosis then determined Previously initiated therapy with asymptomatic apical periodontitis. Case Management : Tooth # 36 treated with one visit root canal treatment, Treatment is continued with fiber post installation and crown, patients no longer complain of pain after treatment. Teeth function normally again. Conclusion: One visit root canal treatment can successfully eliminate patient complaints and restore tooth function.
The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus Tamara Yuanita; Dian Agustin Wahjuningrum; Mega Selvia
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.67-71

Abstract

Background: Lactobacillus acidophilus is a gram-positive rod-shaped bacteria which mostly causes chronic caries lesions. In the process of cavity preparation it is reported that it is not possible to remove all microorganisms and even bacteria can survive even after removal of the necrotic tissue. Efforts to stop the growth of these bacteria are by chemical cavity cleanser using a 5% NaOCl, but this material have disadvantages including irritating periradicular tissue, unpleasant odor, and toxicity. Due to its disadvantages, it is hoped that natural ingredients can be used as alternatives. Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial ability with a concentration of 6% according to the non-toxic minimum kill concentration of Lactobacillus acidophilus. Purpose: To analyze the difference in antibacterial power between the extract of cocoa bark with a concentration of 6% compared to 5% NaOCl against Lactobacillus acidophilus. Methods: This study was a laboratory experimental in vitro with a post test-only control group design. Using diffusion method against Lactobacillus acidophilus grown in tubes containing BHIB, then cultured in a petridish which contains a nutrient agar and is divided into 2 parts extract cocoa peel extract 6% and NaOCl 5%, then each petridish given a paper disc that has been poured 10μl by each material, then incubated inthe incubator for 2x24 hours at 37oC and observe the diameter of the inhibition zone formed using a caliper. Results: The average diameter of the inhibition zone formed in the 6% cocoa peel extract was 11.8375 mm and NaOCl 5% was 26.0344 mm against Lactobacillus acidophilus. Conclusion: There is a difference in antibacterial power between 6% cocoa peel extract and 5% NaOCl against Lactobacillus acidophilus.
White pomegranate (Punica granatum) peels extract bactericidal potency on Enterococcus faecalis Eric Priyo Prasetyo; Widya Saraswati; Dian Agustin Wahjuningrum; Latief Mooduto; Rizka Firdaus Rosidin; Evelyn Tjendronegoro
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.84-88

Abstract

Background: Enterococcus faecalis (E. faecalis) is the most resistant bacteria in the root canals and one of the causes of recurrent endodontic treatment. Enterococcus faecalis was not the only bacteria found in failed endodontic treatment but these bacteria can thrive in unfavorable environment. White pomegranate (Punica granatum) is known for its flavonoids and tannins that function as antimicrobial agent. White pomegranate extract is potential for use as disinfection or irrigation material. Purpose: This aim of this study was to determine the bactericidal potency of white pomegranate (Punica granatum) extract on E. faecalis growth. Methods: This study was an in vitro experimental observation. E. faecalis was obtained from stock culture taken from the root canal of recurrent endodontic treatment. E. faecalis from the serial dilution were cultured in blood agar media. Antibacterial potency was determined by colony calculation of E. faecalis growth in blood agar in colony forming unit (CFU) and conducted in 6 replications for each concentration group. Statistical analysis was done using one-way analysis of variance at 5% significance level. Results: White pomegranate peels extract concentrations of 3.125%, 6.25%, 12.5% and 25% provide significant decrease in the number of E. faecalis colony compared to the control group (p<0.05). No bacterial growth was found on 25%, 50% and 100% concentration. Conclusion: The potent minimal bactericidal concentration of white pomegranate peels extract on E. faecalis was 25%.
A Mixture of Ceramic Biomaterials (Hydroxyapatite and β-Tricalcium Phosphate) and Chitosan as a Scaffold For Critical Sized Defect Bone Dian Agustin Wahjuningrum; S. Setyabudi; Fery Setiawan; Dwikora Novembri Utomo; Ria Chusnita; Andi Syahrimayani; Arif Rahman Nurdianto
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.93-97

Abstract

Background: Bone is a living tissue that undergoes a continuous regeneration-remodeling process and the second largest organ implanted after the blood transfusion process. Bones can heal completely, but Critical Size Defects (CSD) require graft materials to support the healing process. There are several graft materials, namely: autologous, allogenous, xenograft, and alloplastic material with their respective advantages and disadvantages through the properties: osteogenesis, osteoconduction, osteoinduction, and others. One of the alloplastic materials is Hydroxypatite/HA and β-Tricalcium Phosphate/β-TCP widely used in the grafting process. HA has the disadvantage of having a low degree of solubility, while β-TCP has a high solubility level when exposed to body fluids. Purpose: To explain the mixture of ceramic biomaterials (Hydroxyapatite and β-Tricalcium Phosphate) and Chitosan as a Scaffold for Critical Sized Defect Bone. Methods: This study used article based review from some journals that contain of the review about HA and -TCP. Results: The CSDs are condition where the bone can not heal by itself. It needs bone graft to bridge the heal of CSDs. One of the transplant materials is ceramic biomaterials contains of HA and β-TCP. Each of material has its strengths and weaknesses so that mixture of these ingredients will increase the positive effects and reduce the negative effects of each ingredient. Conclusion: CSD healing requires a scaffold that mimics cancellous bone in the healing process of bone defects played by the mixture of BCP as a bioceramic material and chitosan as a natural biopolymer with low toxicity and high biocompatibility.
The Use of Artificial Intelligence in New Normal Era Against Pandemic COVID-19 in the Field of Health Services Dian Agustin Wahjuningrum; Fery Setiawan; Setyabudi; Heni Puspitasari; Ahmad Yudianto; Agung Sosiawan; Arif Rahman Nurdianto; Swadheena Patro
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.12-19

Abstract

Background: Indonesia is currently experiencing Coronavirus Disease-19 (COVID-19) pandemic which affects all sectors of life, therefore Indonesia is starting to prepare for New Normal era, including in health care sector. COVID-19 was previously known as novel Coronavirus Disease-19 (nCoV-19), where World Health Organization (WHO) declared it as a global pandemic in March 2020. New Normal era is an era of coexistence with COVID-19, resulting in new habits that were rarely or never practiced before. One practice that can be applied in health care sector is to use Artificial Intelligence (AI) to treat COVID-19 patients to prevent casualties for health workers. Health care providers are prone to contract aerosol and droplet transmission while treating patients, resulting in cytokine storm, acute respiratory distress syndrome (ARDS), and acute lung injury (ALI), which leads to death. Purpose: This mini review study used to explain the using of artificial intelligence in the new normal era against pandemic COVID-19 in the field of health services. Reviews: This pandemic COVID-19 restricts all of the communication between person to person. It also challenged the health service sector because the health service sector is the first line to combat the COVID-19. It is now popular to use artificial intelligence. This using can be used to help the health service sector in combating the COVID-19 pandemic especially in this new normal era. Conclusion: COVID-19 pandemic affects all aspects of life, especially health care sector, therefore AI use is important to prevent the deaths of health workers because of this pandemic.
A case study of informed consent in Indonesian Law Number 29, 2004 Agung Sosiawan; Vera Rimbawani Sushanty; Dian Agustin Wahjuningrum; Fery Setiawan
Dental Journal Vol. 56 No. 1 (2023): March 2023
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i1.p1-6

Abstract

Background: Informed consent is an agreement between the doctor/the provider of medical services and the patient/the recipient of medical services. This relationship between these parties has changed from a paternalistic to a contractual relationship due to technological shifts. Doctors are obliged to notify the patient of all the risks and benefits of a procedure while respecting their autonomy by not intervening the decision-making process. This article will look at three government and academic hospitals in Surabaya, as informed consent has to be practiced in all medical settings. Purpose: This study aims to review the role of informed consent according to Law Number 29, 2004. Review: This study aims to discuss the characteristics of informed consent under Law Number 29, 2004, because there are too few articles addressing this issue. It also explains the roles of the patient and the doctor/dentist in informed consent according to this piece of legislation. Conclusion: According to Article 184, informed consent provides vital evidence that can be used to hold doctors and dentists legally accountable because it contains information about standard operating procedures (SOPs) that medical professionals are legally required to follow. Guidelines for informed consent are given in Law Number 29, 2004, Article 45, paragraph 2.
Toxicity test of bioceramic biphasic calcium phospate (BCP) Sr-Ag doping as bone graft in BHK-21 fibroblast cells Wahjuningrum, Dian Agustin; Setyabudi, Setyabudi; Chusnita, Ria
Conservative Dentistry Journal Vol. 13 No. 1 (2023): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i1.2023.33-36

Abstract

Background: Bone graft is a substitute material that is used to assist reconstruction, stabilize the structure and bonds in bone, stimulate the osteogenesis process and as a healing bone defect. One type of bone graft that has good osteoinductive and bicompatibility is alloplast which is a synthetic calcium phosphate compound. The most frequently used Calcium Phosphate groups are Hydroxyapatite (HA), β-Tricalcium Phosphate (β-TCP), and Biphasic Calcium Phosphate (BCP). In this study the material used was BCP doping Sr2+ and Ag+. Strontium ions (Sr2+) can increase osteoblast activity, reduce osteoclast activity and cytokine production, improve osteointegration, and minimize fractures. Ag+ ion has the ability as an antibacterial agent. Purpose: To explain and prove the toxicity of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as bone graft on BHK-21 fibroblast cells. Methods: This type of research is a laboratory experiment with a post-test only control group design. Treatment with Biphasic Calcium Phosphate (BCP) doped Sr-Ag with concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm in BHK-21 fibroblast cell culture. Results: The percentage of fibroblast cell life at concentrations of 200 ppm, 180 ppm, 160 ppm, 140 ppm, 120 ppm, 80 ppm, 40 ppm, 20 ppm, 10 ppm, 5 pmm, 2.5 ppm, 1.25 ppm, 0.625 ppm, respectively, the percentage of live cells was 38% 44%, 46%, 50%, 52%, 65%, 69%, 71%, 72%, 75%, 77%, 81%, and 87%. The parameter used in this toxicity test is CD50. Conclusion: The results of the toxicity test of bioceramic Biphasic Calcium Phosphate (BCP) doped Sr-Ag as a bone graft showed a toxic and non-toxic effect on BHK-21 fibroblast cells at certain concentrations.
A review of tissue engineering in regenerative endodontic treatment Prasetyo, Eric Priyo; Wahjuningrum, Dian Agustin; Sampoerno, Galih; Sukandar, Wilson; Shariz Bin Sharizal, Shafy; Paidal, Nurfahira; Fadiyan Amriel, Menza; Gunawan, Nathania Elita; Suardita, Ketut; Tjendronegoro, Evelyn
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.1-5

Abstract

Background: Regenerative endodontic treatments are classified based on approach in the procedures and principles. One is cell-free approach that involves a revitalization procedure to achieve continued root growth while the other is cell-based approach which involves pulp/dentin regeneration through isolation and ex vivo expansion of stem cells seeded in the scaffold and then transplanted into the canal space. Technically, the cell-free approach is simpler than cell-based approach because the former does not concern about external stem cell isolation and culture. Currently, the gold standard and the one that has been clinically performed is cell-free approach. Cell-based approach is still not a standard because currently the cell-based approach is still in the clinical trial stage. Purpose: to evaluate the biological basis and clinical protocols recently used in regenerative endodontic treatment and discuss potentially future treatment approaches. Review: The literature review was searched in PUBMED with the keywords: immature permanent teeth, pulp revascularization, stem cells and regenerative endodontics. The regenerative endodontic treatments with various procedures and techniques result in a significant increase in root length and dentinal wall thickness. Stimulation of stem cells in apical root canal system is required to induce tissue formation and continued root maturation. Conclusion: The success of regenerative endodontic therapy relies on the development of a technique that enables clinicians to create functional pulp tissue within cleaned and shaped root canal systems. It is hoped that further research studies are required to define advantages and limitations of cell-free and cell-based approach.
Esthetic crown lengthening treatment on the maxillary anterior teeth Wahjuningrum, Dian Agustin; Sampoerno, Galih; Prasetyo, Eric Priyo; Saraswati, Widya; Setyabudi; Saravanan, Pravinna; Riyadi, Tiara Lathifah; Ambarwati, Venny Lusanda
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.34-37

Abstract

Background: Asymmetrical smile design due to uneven gingival margin heights of the anterior upper teeth can lead to aesthetic issues and inadequate crown restorations, necessitating crown lengthening treatment. Purpose: The objective of this case report is to demonstrate the outcomes of crown lengthening to achieve improved aesthetics and symmetric gingival margin heights. Case: A 16-year-old female patient sought treatment for decayed left maxillary anterior teeth. Clinical examination revealed a non-vital tooth (tooth 21) with decay and excessively low gingival margins, along with asymmetrical gingival heights between the incisors and canines. Radiographs indicated radiolucent mass in the pulp chamber. Case Management: Crown lengthening was performed on teeth 12, 11, 21, and 22, and apexification was conducted on tooth 21 using a customized fiber post and lithium disilicate crown. Follow-up evaluations showed no complaints, well-maintained crowns, and healthy gingiva. Conclusion: Crown lengthening is feasible for anterior teeth with low gingival margins, enhancing clinical crown length to support aesthetic and final restorations in maxillary anterior teeth.
Co-Authors Adityatama, Anneke Paramita Agung Sosiawan Ahmad Yudianto Ambarwati, Venny Lusanda Amit Bhardwaj Ananta Tantri Budi Andi Syahrimayani Andi Syahrimayani Anuj Bhardwaj Ari Subiyanto Ari Subiyanto, Ari Arif Rahman Nurdianto Arif Rahman Nurdianto Budiharjo, Adrianto Chang, Nicole Ava Chusnita, Ria Cornelia Melinda Adi Santoso Dalhar Hakiki Darmawan Darwis Devi Eka Juniarti Dwikora Novembri Utomo Dyanita Hasri Eric Priyo Prasetyo Eveline Yulia Darmadi Evelyn Tjendronegoro Evelyn Tjendronegoro Fadiyan Amriel, Menza Febriastuti Cahyani Fery Setiawan Firmansyah, Awang Bagus Galih Sampoerno Goenharto, Aurelia Gunawan, Nathania Elita Handoko, Jessica Cristy Heni Puspitasari Ira Widjiastuti Ivon Dewi Setianingrum Karlina Samadi Ketut Suardita, Ketut Kevin Prayogo Kratika Mishra Kun Ismiyatin Kurniati, Mala Latief Mooduto, Latief M Rulianto Marpaung, Grace Julieta Mega Selvia Monika Werdiningsih Nabiela Rahardia Paidal, Nurfahira Prasita, Shafa R. Roulianto Ria Chusnita Riyadi, Tiara Lathifah Rizka Firdaus Rosidin RM Coen Pramono D Saptaningrum, Mardikaning Riasta Saravanan, Pravinna Setyabudi Setyabudi Setyabudi Setyabudi Setyabudi Setyabudi, Setyabudi Shariz Bin Sharizal, Shafy Sheetal Khandelwal Shivani Bhardwaj Sholeh Ardjanggi Sukandar, Wilson Sukaton, Sukaton Susanto, Dany Agus Sutikno, Antonius Edwin Swadheena Patro Tamara Yuanita Tantri Wismayaning Radito Tjendronegoro, Evelyn Ulfadi , Bakhiitah Thufailah Putri Vera Rimbawani Sushanty Widya Saraswati Wiratno, Amelia Rahma Yemy Ameliana Yessy Andriani Fauziah Yuline Krishartini