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All Journal HAYATI Journal of Biosciences Journal of Biomedicine and Translational Research PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Mandala Pharmacon Indonesia Pharmaceutical Sciences and Research (PSR) Jurnal Farmasi Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Rahmasari, Ratika
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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In vitro Antiviral Activity of Cat’s Claw (Uncaria tomentosa) bark Extract Against Human Influenza A Virus Makau, Juliann N; Talaam, Keith K; Odiwuor, Mike; Majanja, Janet M; Musenjeri, Sophia; Rahmasari, Ratika; Khamadi, Samoel A; Watanabe, Ken
Pharmaceutical Sciences and Research Vol. 12, No. 2
Publisher : UI Scholars Hub

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Abstract

Influenza A viruses mutate very fast and this leads to the development of resistance to antivirals, hence the need to find new therapeutics. Due to their extensive traditional use, natural products are crucial resources for the discovery of drugs. Cat’s claw (Uncaria tomentosa), is a tropical vine known for various health benefits and in this study we evaluated its anti-influenza activity. Pulverized material of the stem bark was extracted using 80% ethanol, hot water, and 1% sodium bicarbonate. The extracts were dried and dissolved in dimethylsulfoxide before testing for cytotoxicity and antiviral activity using crystal violet assay. The extracts exhibited antiviral activity with a 50% inhibitory concentration range of 2.3 - 6.2 µg/mL. The 1% sodium bicarbonate extract had the least 50% cytotoxic concentration of 125 µg/mL and was used for further analyses. It was effective against several human influenza A virus strains including an oseltamivir-resistant clinical isolate of the 2009 H1N1 pandemic influenza. Time-of-addition experiments showed significant suppression of virus replication when the virus was pretreated with the extract before infecting to cells and also when the virus and extract were co-administered to the cells; suggesting that the extract was inactivating the virus. Oseltamivir was used as a positive control and only suppressed late stages of virus replication. These results were consistent with the hemagglutination inhibition assay data which showed direct virucidal activity on influenza virus particles. Our data demonstrates possible future use of Cat’s claw in influenza management. However, more investigation is needed to identify the bioactive components.
Co-Authors Adrian Hartanto, Adrian Ahsanal Kasasiah Annajla, Fathina Annisa, Syifa Ariestanti, Donna Maretta Arifianti, Ayun Arifianti, Ayun Erwina Berna Elya Chairunissa, Ananda Hanny Forestrania, Roshamur Cahya Forestrania, Roshamur Cahyan Hanandi, Sharon Herman Suryadi Hermosaningtyas, Anastasia Aliesa Indah Laily Hilmi Irianti, Marina Ika Jantan, Ibrahim Jekmal Malau Khamadi, Samoel A Kurnia Sari Setio Putri Kurnia Sari Setio Putri, Kurnia Sari Setio Larosa, Febri LS Majanja, Janet M Makau, Juliann N Makau, Juliann Nzembi Meilani, Nanda Diva Muhareva Raekiansyah Musenjeri, Sophia Odiwuor, Mike Okti Nadia Poetri Putri Mulia, Ocha Rani Sauriasari, Rani RR. Ella Evrita Hestiandari Sari, Rifta Septi, Annisa Frastica Septiarini, Tri Hastuti Siti Rohmah Sutriyo Syahdi, Rezi Riadhi Syamsu Nur, Syamsu Talaam, Keith K Urbaningrum, Lestari Mahardika Watanabe, Ken Wilbert Wylie Zahra, Aliya Azkia Zahro, Aurora Fatimatuz