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Evy Diah Woelansari
Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Surabaya Surabaya
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Jaringan Laboratorium Medis

 1 
Perbandingan Pemeriksaan Skrining Antibodi (Indirect Coombs Test) dengan Direct Coombs Test pada Pasien dengan Riwayat Transfusi Darah Berulang Arta, Shela Yuni; Suhariyadi, Suhariyadi; Woelansari, Evy Diah; Rahayuningsih, Christ Kartika
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13643

Abstract

Repeated blood transfusions can increase risk of alloimmunization and autoimunization, which causes formation alloantibodies and autoantibodies. The presence of these antibodies can make cross-matching more difficult and increase the possibility of hemolytic transfusion risk. Antibody screening tests (Indirect Coombs Test) and Direct Coombs Test play a role in detecting the presence of alloantibodies and autoantibodies in patient with a history of repeated transfusion. The aim of the study was to determine the diferences in results between antibody screening (Indirect Coombs Test) and Direct Coombs Test in patients receiving repeated blood transfusions. This study used a quasi-experimental design with a cross-sectional approach. The subjects were patients with a history of blood transfusions more than 5 times at RSUD R.T. Notopuro Sidoarjo totalling 60 samples. The data obtained were then analyzed statistically to see if there were any significant differences between the two types of examinations. The results obtained showed a significant difference between the results of the antibody screening test (ICT) and the Direct Coombs Test (DCT) (p-value 0.000), that more patients with repeated blood transfusions had positive DCT results (75%). Some patients showed positive results on one or both types of tests, indicating the presence of alloantibodies or autoantibodies. ICT and DCT tests provide complementary information in detecting antibodies in patients with repeated transfusions. The use of both is important in improving the safety of blood transfusions and minimizing the risk of hemolytic reactions.
Co-Authors , Suhariyadi ., Pestariati A.A. Ketut Agung Cahyawan W Abror, Yogi Khoirul Adelia Gita Prasasti Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Anggraini, Anita Dwi Anita Dwi Anggraini Aprilyadi, Nadi Arifin, Syamsul Diah Arta, Shela Yuni Aryati Aryati Ayu Puspitasari, Ayu Cheppy Juwita Ayuningrum Christ Kartika Rahayuningsih Christiyaningsih, Juliana Diah Titik Mutiarawati Edy Haryanto Eka Safitri, Devayanti Endarini, Lully Hanni Entuy Kurniawan Kurniawan Evi Yunita Nugrahini Fadia Rakhmalia Fortuna Aliza Dewi Ginarsih, Yuni Handayati, Anik Hariyanto Hariyanto Hermiyanti, Pratiwi Ira Puspita Sari Ira Rahayu Tiyar Istanto, Wisnu Istiqomah Istiqomah istiqomah istiqomah Juliana Christyaningsih Kasiati Kiaonarni O.W Krihariyani, Dwi Kumahartati, Intan Lembunai Tat Alberta Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi Mamik Mamik Mamik Mamik Minarti Minarti Minarti Minarti Mohd Nazil Salleh Mujayanto Museyaroh, Museyaroh Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Niswata, Navila Arfianti Novikasari, Caturizkyana Nur Hatijah Nurwening TW P, Teresia Retna Pertiwi, Resty Cahya Prasasti, Adelia Gita Purnomowati, Anik Purwati Purwati Puspa Wardani Rahayu Sumaningsih Rahayuningsih, Christ Kartika Rahayuningsih, Christ. Kartika Rahma Yuliana, Putri Ramadhany, Rieke Dwi Retno Sasongkowati Retno Sasongkowati Retno Sasongkowati Rieke Dwi Ramadhany Riyanto, Digdo Rizqiyah, Aliya Ayu Salsabiil Rochman Alifiah Sasongkowati, Retno Sri Sulami Endah Astuti Sri Utami Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Sumasto, Hery Syafira, Nurah Teta Puji Rahayu Widiastutik, Dyah Wisnu Istanto Yohanes Kambaru W Zerlinda, Nur