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Skrining Fitokimia dan Aktivitas Antituberkulosis Lichen Usnea sp.: Phytochemical Screening and Antitubercular Activity of Lichen Usnea sp. Riga, Riga; Agustini, Dewi Meliati; Shidiq, Gin Gin Ash; Ikhsan, Muhammad Habibul
Jurnal Sains dan Kesehatan Vol. 6 No. 5 (2024): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v6i5.2381

Abstract

This research aims to identify fractions inhibiting the growth of M. tuberculosis. The sample in this study was Usnea sp. dissolved in methanol followed by extraction with EtOAc. Antituberculosis testing was carried out using the Microscopic Observation Drug Susceptibility (MODS) method on both extracts (methanol and ethyl acetate) with media as negative control and media and Polymyxin, Amphotericin B, Nalidixic acid, Trimethoprim and Azlocillin (PANTA) as positive control. Observations of them were carried out until the 14th day using a microscope. The observation results showed that the EtOAc extract had a strong minimum killing power against M. tuberculosis isolate H37Rv, while the methanol extract showed a moderate minimum killing power against M. tuberculosis isolate H37Rv at a concentration of 250 ppm. Interestingly, the antitubercular activity using MODS method from Usnea sp. extract. Was firstly studied in this research. Keywords:          Lichen, M. Tuberculosis, MODS, Usnea sp.   Abstrak Tujuan studi ini yaitu untuk mengidentifasi fraksi yang dapat menginhibisi pertumbuhan bakteri M. tuberculosis. Sampel yang diujikan adalah ekstrak pekat Lichen Usnea sp. yang dilarutkan dengan metanol yang selanjutnya diekstraksi dengan etil asetat. Aktivitas antituberkulosis ditentukan dengan metode Microscpy Observation Drug Susceptibility (MODS) terhadap kedua ekstrak (metanol dan etil asetat). Media kosong digunakan sebagai kontrol negatif dan kontrol positif berupa media dan Polymyxin, Amphotericin B, Nalidixic acid, Trimethoprim and Azlocillin (PANTA). Isolat bakteri yang digunakan adalah M. tuberculosis isolat H37Rv. Pengamatan dilakukan sampai hari ke-14 menggunakan mikroskop. Hasil pengamatan menunjukkan bahwa ekstrak etil asetat memiliki daya bunuh minimum yang kuat terhadap M. tuberculosis isolat H37Rv, sementara ekstrak metanol menunjukkan daya bunuh minimum yang sedang terhadap M. tuberculosis isolat H37Rv pada konsentrasi 250 ppm. Menariknya, penelitian uji aktivitas antituberkular menggunakan metode MODS dari ekstrak Usnea sp. pertama kali dilakukan pada penelitian ini. Kata Kunci:         Lichen, M. Tuberculosis, MODS, Usnea sp.
Implementation of Sekolah Penggerak Programme to Address Chemistry Teachers Competency on Design PjBL-based Digital Teaching Materials in Padang Suryani, Okta; Sari, Trisna Kumala; Riga, Riga; Mawardi, Mawardi; Putra, Ahadul
Hydrogen: Jurnal Kependidikan Kimia Vol. 12 No. 5 (2024): October 2024
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v12i5.12952

Abstract

Teaching materials for Sekolah Penggerak should be prepared based on a creative thinking learning model such as project-based learning (PjBL). However, based on the needs and situation of teachers towards the implementation of PjBL-based learning, implementation of PjBL in learning is still low, since the teachers in Padang need the advance training of preparation in PjBL learning material. The Community service activities have been carried out to provide experienced chemistry teachers in developing teaching materials based on Project-Based Learning. The implementation methods carried out in this activity are: (1) Analyzing the needs and situation of teachers towards the implementation of PjBL-based learning through questionnaires distributed, (2) Submission of material to service participants related to the PjBL learning model and syntax in this learning model explained by the speaker, then (3) Teachers are given the task of compiling and designing chemistry LKPDs using the PjBL model, (4) Teachers will present the results of the LKPD design compiled, and (5) evaluation of activities. The results of the service activities carried out can provide experience and understanding for chemistry teachers in Padang city regarding the PjBL learning model. Participants who attended this service activity could prepare chemistry teaching materials using the PjBL model so that teachers could be more innovative and creative. This activity service can provide motivation for teachers to prepare teaching materials in accordance with the demands of the Kurikulum Merdeka, especially in the implementation of project-based learning.
The Chemistry Teacher Activities on The Training of Project Based Learning Teaching Materials Digitalisation Suryani, Okta; Sari, Trisna Kumala; Riga, Riga; Mawardi, Mawardi; Yuhelman, Nofri; Nasra, Edi; Velly, Aglin; Rahmad, Nafisah Yulia
Hydrogen: Jurnal Kependidikan Kimia Vol. 12 No. 6 (2024): December 2024
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v12i6.13495

Abstract

This activity is the second year of community service which is a continuation of training activities and making digital teaching materials to support project-based learning (PjBL) in sekolah penggerak for chemistry teachers in Padang city. This second year of community service activities has the theme of training to digitise project-based learning teaching materials developed at the Chemistry Musyawarah Guru Mata Pelajaran (MGMP) in Padang city. This activity aims to provide direction to Padang city chemistry teachers in implementing digital teaching materials. This community service was carried out in the lecture building of FMIPA Universitas Negeri Padang on 9 August, 16 August, and 23 August 2024. The implementation methods carried out in this community service activity are: (1) conducting a needs analysis, knowing the situation of teachers towards the implementation of PjBL-based learning through questionnaires distributed, (2) delivering material to community service participants related to PjBL and an overview of teaching materials that have been designed using the PjBL learning model explained by the speaker, then (3) delivery of materials and discussions related to differentiated learning with the PJBl learning model, and (4) delivering material related to the digitalisation of project-based teaching materials or PjBL using liveworksheet. The results of the service activities carried out can provide motivation, provide experience, and understanding for Padang city chemistry teachers regarding the digitalisation of project-based learning teaching materials so that chemistry teachers in Padang city can prepare digital chemistry teaching materials, especially with liveworksheets using the PjBL model that can assist learning activities so that learning can run effectively and efficiently in accordance with the demands of the curriculum and the times.