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Journal : Journal Health Applied Science And Technology (JHAST)

Perbedaan Jumlah Trombosit Pada Teknik Pengambilan Darah Vena menggunakan Spuit Dan Vacumtainer Bastian Darwin; Aristoteles; Dewi Hartati
jurnal kesehatan terapan sains dan teknologi Vol 1 No 2 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i2.12

Abstract

Platelets are blood cells that play an important role in homepstatis. Platelets attach the endothelial layer of torn blood vessels (wounds) by forming a platelet plug. Platelets do not have a cell nucleus, measuring 1-4, and the cytoplasm is blue with purple-reddish granules. Platelets are derived from megakaryocytic, which are derived from the cytoplasm fragments of megakaryocytic. 150.000-350,000 platelets /ml of blood platelet granules contain blood clotting factors, Adenosine Diphosphate (ADP). Adenoxine Triphoxphate (ATP, calcium, serotonin, and catecholamines. Most of them play a role in stimulating the start of the blood clotting process. The lifespan of platelets in circulating blood is about 8-10 days. The purpose of this study was to determine differences in the number of platelets depending on the technique of taking venous blood using a syringe and a vacumtainer. The type of research used is cross sectional, which was carried out in the laboratory of Puskesmas Dempo on 05-01-2022. The sample used was EDTA blood. The number of samples studied was 30 samples with two treatments using a non-probability sampling technique. Based on the results of the examination of the number of platelets in the technique of taking venous blood using a syringe and a vacumtainer using the hematology analyzer, the average value for the syringe method was 353,27 /ml of blood and the vacumtainer method had an average value of 345,27 /ml 0f blood p= 0.612 was obtained from data analysis using the paired sample test. The conclusion of this study is that there is no difference between the number of platelets dependen on the technique of taking venous blood using a syringe and a vacum.
PERBEDAAN HASIL BAKTERI TAHAN ASAM METODE ZIEHL NEELLSEN DAN TES CEPAT MOLEKULER (TCM) DENGAN LAMA PENYIMPANAN 48 JAM Aristoteles; Yuliana Maya Sari; Denny Juraijin
jurnal kesehatan terapan sains dan teknologi Vol 2 No 1 (2024): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v2i1.33

Abstract

Background: Tuberculosis (TBC) is an infectious disease caused by Mycobacterium tuberculosis, a dorect human-to-human infectious disease not caused by witchcraft or curses TB germ most often attack the lungs but can also attack other organs such as the lymph nodes, bones, brain, skin and others. The diagnosis of pulmonary TB is established based on the clinical picture, physical examination, radiological picture, laboratory examination and tuberculin test. The diagnosis of pulmonary tuberculosis currently used routinely in labpratories including hospitals and health centers is a bacteriological diagnosis using acid-fast bacillus microscopic techniques (BTA) and molecular rapid test (TCM). TCM examination is a molecular detection methode based on nested real-time PCR. The use of TCM is a priority for TB examination because it has several advantages, including: High sensitivity, fast and results can be known in approximately 2 hours. Objectives: To determine differences in the results of acid-fast bacteria Ziehl Neelsen method and the molecular rapid test (TCM) with a storage time of 48 hours. Method: This type of research is pure experiment. The research was conducted in the Celikah Health Center laboratory in February 2023 with a total sample of 5 smear samples (+1). Result: The results showed that there was no difference in the tesults of acid-fast bacteria between the ziehl neelsen method and the molecular rapid test (TCM) for 48 hours of storage. The wilcoxon test results obtained a value of p = 0,157. Conclusion: The molecular rapid test method has better sensitivity and specificity in the diagnosis of tuberculosis thab microscopic examination using ziehl neelsen staining.
Perbedaan Hasil Pemeriksaan Leukosit Sebelum Dan Sesudah Pembarian Jus Jambu Biji Merah Aristoteles; Indah Sari
jurnal kesehatan terapan sains dan teknologi Vol 3 No 1 (2025): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v3i1.73

Abstract

Background: Leukocytes are blood cells that contain a nucleus, also called white blood cells. Leukocytes are one of the blood components that play a role in protecting against infections caused by viruses, bacteria, or metabolic processes of toxins. There are five types of leukocytes, each of which has a specific function in fighting pathogens, namely neutrophils, eosinophils, basophils, monocytes, and lymphocytes. Leukocytes function as the body's defense system by being transported to cells that experience inflammation such as due to oxidation. Physiological stimulation that can increase the number of leukocytes is exercise, emotion, and extreme temperatures. Red guava is one of the fruits that has many benefits, the benefits of red guava include containing 228 mg/100 grams of vitamin C, rich in folic acid, maintaining the immune system, and preventing anemia. Research Objective: to determine the difference in leukocyte examination results before and after administration of red guava juice. Research Method: Experiment. The study was conducted on March 1, 2023 at Bunda Hospital Palembang and sampling was carried out at the Muhammadiyah Institute of Health Sciences and Technology Palembang, totaling 30 EDTA blood samples with two treatments. Results: the results of the study had an average value of the number of leukocytes before giving red guava juice, namely 6,596 cells/mm3 and the number of leukocytes after giving red guava juice, namely 5,716 cells/mm3. The results of the paired T-test obtained a p value = 0.122. Conclusion: Leukocyte examination can be carried out before or after giving red guava juice.