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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Kebidanan Midwiferia JURNAL PENDIDIKAN TAMBUSAI JURNAL KESEHATAN PERINTIS EDUKATIF : JURNAL ILMU PENDIDIKAN JURNAL ANALIS LABORATORIUM MEDIK Anakes: Jurnal Ilmiah Analis Kesehatan Jurnal Kesehatan Tambusai Jurnal Ilmiah Kesehatan Masyarakat Hasil Karya Aisyiyah Untuk Indonesia JURNAL INSAN CENDEKIA
Shafriani, Nazula Rahma
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Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Anakes: Jurnal Ilmiah Analis Kesehatan

 1 
Uji Efektivitas Konsentrasi Kacang Kedelai Sebagai Media Alternatif Terhadap Pertumbuhan Jamur Candida albicans Rahayu, Putry Shofiyani; Solikah, Monika Putri; Shafriani, Nazula Rahma
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2720

Abstract

Candida albicans is a type of pathogen fungus that can cause opprtunistic infections, especially in individuals with low immune systems.The gold standard for Candida albicans identification is culture examination using Sabouraud Dextrose Agar (SDA) media, but this is relatively media are needed that are more economical, easily available, and able to support optimal fungal growth. This study aims to determine the effectiveness of soybean (Glycine max) concentration as an alternative media to replace SDA for the growth of Candida albicans. This research was conducted experimentally with a quantitative approach, using a variet of soybean concentrations (5%, 10%, 15%, and 20%) which were tested for growth macrocopically, microscopically (KOH 10% and LPCB), calcualtion of the number of colonies and concentration effectiveness. The results showed that soybean media supported the growth of Candida albicans, with 20%concentration giving effective results resembling growth on SDA media. Based on these results, soybean has the potential as an alternative medium with an economical price for Candida albicans fungal culture in microbiology laboratories.   Keywords: Candida albicans, soybean, alternative media, Sabouraud Dextrose Agar.  
Pengaruh Waktu Penyimpanan Ekstrak Buah Naga (Hylocereus polyrhizus) sebagai Pewarnaan Alternatif Eosin Pada Sediaan Hepar Mencit (Mus musculus) Ramadhani, Falah Aiken; Rahmawati, Yeni; Shafriani, Nazula Rahma
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2741

Abstract

Histotechnics aims to identify tissue structure through a staining process, one of which uses eosin which has dangerous side effects and is expensive. The purpose of this study was to test the stability of dragon fruit extract (Hylocereus polyrhizus) as a substitute for eosin staining in mouse liver tissue (Mus musculus) with varying storage times of 10 days and 15 days. This research method used a quasi-experimental with a descriptive approach. Staining was carried out on 12 preparations divided into three groups: control (eosin), 10-day extract, and 15-day extract. The results showed that the best staining was obtained in the control group for total scoring 60, while the 10-day extract group produced a fairly good total scoring staining quality 31, and the 15-day group showed a decrease in total scoring staining quality 23. The results of this study revealed that dragon fruit extract has the potential as an alternative eosin staining, especially after 10 days of storage, but its effectiveness decreased after 15 days of storage. Further research recommendations include optimizing extract stability to extend the shelf life of natural dyes.  Keywords: Dragon Fruit (Hylocereus polyrhizus), Eosin, Histology..
Co-Authors Amri, Shofy Farha Aulia Andika, Lalu Wahyu Gde Annisa, Tata Arif Bimantara Astuti, Tri Dyah Bimantara , Arif Cahayani, Ica Putri Citra Agung Hiola Damayanti, Tania Dian Retnaningdiah, Dian Faradila nukuhehe, Faradila Faurina Risca Fauzia Fauzia, Faurina Fridhoriansyah, Zuvi Irfani, Farida Noor Kau, Mohamad Abrar Hamdan Kiki, Kiki Maharani, Intan Mu’awanah, Isnin Aulia Ulfah Permana, Riski Aditya Putri, Novita Eka Putria, Wina rahayu, eka puji Rahayu, Putry Shofiyani Raihan, Muhamad Ramadhani, Falah Aiken Ratih, Woro Ummi Refnaldi, Revi Rizkika, Rif'ani Aulia Solikah, Monika Putri Sri Martuti Sumaryanto, Siti Sholikhah Suryanto Suryanto Trecia, Cessy Wahyuntari, Evi Widyantara, Aji Bagus Yeni Rahmawati, Yeni