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Kontaminasi Escherichia coli pada Air Minum Isi Ulang di Wilayah Ciangsana Menggunakan Uji Most Probable Number (MPN): Escherichia coli Contamination in Drinking Water Refills at Ciangsana Region using Most Probable Number Test Mohammad Maulana Fajar; Muhammad Rizki Kurniawan; Dian Rachma Wijayanti
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.4102

Water is a basic need for humans. Human life cannot be separated from water, especially the need for drinking water. Water needs have been sourced from groundwater, river water, rainwater, mountain water, and treated seawater. Water that humans need includes clean and healthy useable water for cooking, washing, and bathing, as well as water that is fit for consumption for drinking purposes. The main use of water and very vital for life is the use of drinking water. Drinking water from depot companies for drinking water refills is an industrial business that processes raw water into ready-to-drink water by consumers. This study aims to determine the presence of Escherichia coli contamination in drinking water sold by refilled drinking water depots in the Ciangsana region. The results of the microbiological analysis showed two out of ten samples were positive for coliform fecal, Escherichia coli.

Extraction and Identification Potent Antibacterial Bioactive Compound of Streptomyces sp. MB 106 from Euphorbia sp. Rhizosphere Dian Rachma Wijayanti; Almatin Puspa Dewi
BIOEDUSCIENCE Vol 6 No 1 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/617898

Background: Actinomycetes are groups of bacteria that play an important role in pharmacy and medicine in their ability to produce secondary metabolites in the form of bioactive compounds with various chemical structures and biological activities. The genus Streptomyces produces more than 70% of all described actinomycetes natural products. In particular, antibiotics represent the largest group. Through mutations, bacteria may survive chemotherapy. Thus its infection is hard to eradicate. Therefore the search for new potent bioactive compounds is important Methods: Isolate preparation, Production, and extraction bioactive compound, Crude extract antibacterial test, Identification of bioactive compound Results: There are antibacterial activities per 100 µl crude extract. Inhibition zone range from 11.5 to 13 mm. Various bioactive compounds detected in n-Butanol extract, There are nine bioactive compounds detected from Streptomyces sp. MB 106 crude extract. The highest was Naphthalene (43.89 %). The lowest was 3-Imino-3h,5h- [1,2,4] Thiadiazolo [3,4-1] Isoindole (1.70 %). Six compounds were reported to have antimicrobial activities butane, 1,1-dibutoxy, naphthalene, nonadecane, docosane, heneicosane, and eicosane Conclusions: Crude extract showed an average of 12 mm inhibition zone against Escherichia coli ATCC 8739. There are nine bioactive compounds from Streptomyces sp. MB 106. Six of them are promising drugs candidate. Further studies on these compounds are essential for future drugs candidate.

HUBUNGAN NLR DENGAN KADAR HS-CRP PADA KASUS DEMAM DENGUEPERIODE TAHUN 2019-2021 DI PRODIA DEPOK Dhian Ekawati; Dian Rachma Wijayanti; Ahmad Fitra Ritonga Ritonga
Binawan Student Journal Vol. 4 No. 3 (2022)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Binawan (LPPM Universitas Binawan)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54771/bsj.v4i3.677

Demam dengue (DD) merupakan penyakit yang disebabkan oleh virus dengue dan ditularkan melalui vektor nyamuk Aedes aegypti dan Aedes Albopictus. Gejala klinis yang ditimbulkan berupa demam 2–7 hari, sakit atau nyeri pada ulu hati serta manifestasi perdarahan. Pada infeksi yang lebih berat disertai dengan leukopenia dan trombositopenia. Neutrophil Lymphocyte Ratio (NLR) dan High sensitive C-reactive protein(Hs-CRP) menjadi salah satu pemeriksaan penunjang DD. Rasio neutrofil limfosit atau NLR memberikan gambaran terhadap respon imun dan derajat inflamasi yang terjadi Hs-CRP merupakan protein fase akut yang diproduksi oleh hati sebagai respon imun nonspesifik terhadap infeksi. Penelitian ini bertujuan untuk mengetahui hubungan NLR dengan kadar Hs-CRP pada kasus DD tahun 2019-2021 di Prodia Depok, sebanyak 30 pasien dengan diagnosis DD menjadi subjek dalam penelitian ini. Penelitian dilakukan dengan desain penelitian cross-sectional. Hasil yang diperoleh dari penelitian ini adalah 16 responden laki-laki dan 14 responden. Usia yang mendominasi adalah kategori lansia awal dan akhir (46-65 tahun) sebanyak 10 responden. Berdasarkan Uji Spearman p-value 0,989 yaitu tidak ada hubungan antara nilai NLR dengan kadar Hs-CRP pada kasus DD Prodia Depok.

Extraction and Identification Potent Antibacterial Bioactive Compound of Streptomyces sp. MB 106 from Euphorbia sp. Rhizosphere Dian Rachma Wijayanti; Almatin Puspa Dewi
BIOEDUSCIENCE Vol 6 No 1 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/617898

Background: Actinomycetes are groups of bacteria that play an important role in pharmacy and medicine in their ability to produce secondary metabolites in the form of bioactive compounds with various chemical structures and biological activities. The genus Streptomyces produces more than 70% of all described actinomycetes natural products. In particular, antibiotics represent the largest group. Through mutations, bacteria may survive chemotherapy. Thus its infection is hard to eradicate. Therefore the search for new potent bioactive compounds is important Methods: Isolate preparation, Production, and extraction bioactive compound, Crude extract antibacterial test, Identification of bioactive compound Results: There are antibacterial activities per 100 µl crude extract. Inhibition zone range from 11.5 to 13 mm. Various bioactive compounds detected in n-Butanol extract, There are nine bioactive compounds detected from Streptomyces sp. MB 106 crude extract. The highest was Naphthalene (43.89 %). The lowest was 3-Imino-3h,5h- [1,2,4] Thiadiazolo [3,4-1] Isoindole (1.70 %). Six compounds were reported to have antimicrobial activities butane, 1,1-dibutoxy, naphthalene, nonadecane, docosane, heneicosane, and eicosane Conclusions: Crude extract showed an average of 12 mm inhibition zone against Escherichia coli ATCC 8739. There are nine bioactive compounds from Streptomyces sp. MB 106. Six of them are promising drugs candidate. Further studies on these compounds are essential for future drugs candidate.

ANALISIS PENERAPAN PRA-ANALITIK DAN ANALITIK KUALITAS SEDIAAN BTA METODE ZIEHL-NEELSEN DI PUSKESMAS KECAMATAN CIRACAS JAKARTA TIMUR Cut Nur Masita; Dian Rachma Wijayanti; Sabarina Elfrida Manik
Jurnal SainHealth Vol. 8 No. 1 (2024): Maret 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i1.14358.1-5

The microscopic examination of BTA with sputum samples aims to detect the onset and monitoring of treatment in tuberculosis. The purpose of this study is to find out the Analysis of the application of six preanalytic and analytical indicators to the quality of acid-resistant basil preparation using the Ziehl-Neelsen method at the East Jakarta Ciracas Subdistrict Health Center, which was conducted in April-June 2023. The type and design of the study are analytical cross-sectional, data analyzed using the Chi-Square test. BTA prepared quality results by performing six indicators, proportionally greater good prepared quality than poor prepared quality with 90% specimen quality percentage, 95% preparation quality, 85% preparation cleanliness, 90% preparation size, 92.5%, and 92.5% preparation accuracy. An analysis of the application of six pre-analytic and analytic indicators to the quality of the acid-resistant basil supply using the Ziehl-Neelsen method at the East Jakarta Ciracas Subdistrict Health Center which includes specimen quality, preparation cleanliness, preparation thickness, and preparation accuracy showed a meaningful relationship. The coloring of the preparedness and the size of the preparedness indicates no meaningful relationship.

PERBANDINGAN KADAR TROMBOSIT PADA PEMBENDUNGAN VENA SELAMA 1 MENIT DAN 2 MENIT: COMPARISON OF PLATTLE LEVELS IN VEIN DAMMING FOR 1 MINUTE AND 2 MINUTES ari ardiyanto sardi; Sabarina Elfrida Manik; Dian Rachma Wijayanti
Jurnal SainHealth Vol. 8 No. 1 (2024): Maret 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i1.14360.11-14

Phlebotomy is the process of drawing blood from the circulatory system through a puncture to obtain a sample. Venous blood collection is done using a tourniquet as a damming device that serves to see the vein under the skin tissue. In adults, one of the veins used is the cubital fossa and in infants using the superficial jugular vein or in the superior sagittal sinus. Platelets are disk-shaped cells with a diameter of 2-5 mm. Under normal circumstances, the number of platelets is about 150,000-450,000 / ml of blood and its life span is about 1- 2 weeks or about 8 days. Platelets are made of phospholipid substances that are important for clotting, maintaining the integrity of blood vessels and repairing damaged small blood vessels. The purpose of this study was to determine the comparison of platelet levels in vein damming at 1 minute and 2 minutes. This study was conducted in the MM2100 industrial area, samples obtained from employees who did Medical Check Up and obtained as many as 30 respondents and 60 samples. The results showed that employees in the MM2100 industrial area with average platelet levels of 274,133/µl of blood at 1 minute and average platelet levels of 278,266/µl of blood at 2 minutes. This study uses the Paired Sample T-Test method, the results obtained with a sig value of 0.229 which means there is no difference in the damming time of 1 minute and 2 minutes on platelet levels.

Isolation and Characterization Of Arbuscular Mychorhiza Fungi from Gaharu Wood (Aquilaria spp.) Rhizosphere Wijayanti, Dian Rachma; Turjaman, Maman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3090

In the last 30 years there have been increasing demand of agarwood. Unlimited hunting of agarwood causing it become threatened species. Aquilaria spp. is the main genus producing agarwood that considered as threathened species. Conserving Aquilaria spp. as germ plasm needs is essential against destruction. Arbuscular mycorrhizal fungi (AMF) have been used as inoculant to increase the growth of Aquilaria spp., but information about the existence of AMF in Aquilaria spp. rhizosphere has not been reported. This research was carried out with objective to identify AMF from Aquilaria spp. rhizosphere. Soil and root samples from rhizosphere of Aquilaria spp. taken from four location that is: Carita (A. malaccensis), Ciapus (A. filaria), Cifor (A. crasna & A. malaccensis), and Bantar Kemang (A. beccariana, A. crasna, & A. malaccensis). Roots staining was conducted with modified Phillips and Hayman procedure (1970). Soils sample was run dry wed and placed in pot culture with Pueraria javanica as the host plant. Spore was separated from pot culture using wet sieving and decanting method continued with centrifugation. The result showed the occurrences of AMF in Aquilaria spp. roots i.e. external hypha, internal hypha, coiled hypha, arbuscules, and vesicles. All AMF colony structure found in Aquilaria spp. roots show asosiation bettwen the plant and AMF. Four species of AMF were found i.e. Glomus sp.1, Glomus sp.2, Gigaspora albida, and Dentiscutata sp. Key Words: Isolation, Arbuscule Mycorrhizae Fungi, Rhizosfer, Agarwood

Hubungan Hematokrit Dan Trombosit Dengan Inflamasi Demam Berdarah Dengue Di Prodia Kebayoran Tahun 2020-2021: Relationship Of Hematocrite And Thrombocytes In Inflammation Case Of Dengue Fever At Kebayoran Prodia 2020-2021 Mulyatmo, Pravita Deasy Setyaningrum; Wijayanti, Dian Rachma; Yansen, Aturut
Binawan Student Journal Vol. 5 No. 3 (2023)
Publisher : Direktorat Penelitian, Pengabdian Masyarakat, Dan Kerjasama Universitas Binawan (DPPMK Universitas Binawan)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Demam berdarah dengue adalah penyakit infeksi virus dengue yang ditularkan oleh nyamuk aedes aegypti. Pemeriksaan laboratorium untuk diagnosis demam berdarah dengue adalah Complete Blood Count (CBC), hs-CRP, Anti-Dengue IgG IgM dan NS1 Antigen. Tujuan penelitian ini adalah untuk mengetahui hubungan hubungan kadar hematokrit dan trombosit dengan tingkat keparahan inflamasi pada kasus demam berdarah dengue di Prodia Kebayoran periode Tahun 2020 – 2021. Jenis penelitian ini adalah analitik kuantitatif dengan desain penelitian cross-sectional Sampel diambil dari populasi menggunakan teknik pengambilan sampel purposive sampling. Jumlah sampel sebanyak 30 pasien Analisis statistik menggunakan Uji Korelasi Pearson dengan SPSS 23.0. Hasil analisis menunjukkan bahwa tidak ada hubungan yang signifikan antara kadar hematokrit dan ada hubungan yang signifikan antara trombosit terhadap tingkat keparahan inflamasi (hs-CRP) (p>0.05, r = 0.030) dan (p<0.05, r = -0.558). Gambaran hasil pemeriksaan laboratorium pada penderita demam berdarah dengue adalah terjadi penurunan jumlah trombosit, peningkatan nilai hematokrit dan peningkatan kadar hs-CRP.

Kelimpahan Mikroba Tanah Asal Rizosfer Tanaman Sukulen Zulaika, Aidha; Wijayanti, Dian Rachma; Safari, Wulan Fitriani
Biota Vol 14 No 1 (2021)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v14i1.340

Plant host symbiosis is close related to soil microbial abundance. Soil microbial abundance will affect soil quality and fertility, thus will improve plant growth. Studies on soil microbial abundance in succulent plant rhizosphere, especially in Indonesia, are scarce. This study aims to observe soil microorganisms' existence and their abundance on succulent plant rhizosphere. This research used two primary methods to isolate Arbuscule Mycorrhizal Fungi (AMF) and actinomycetes. The spore extraction (soil separating) method was used to obtain AMF spores, followed by the root staining method to observe AMF infection on the plant roots. Serial dilution and pour plate method were used for isolation of Actinomycetes. The isolation results showed a high number of Actinomycetes distribution up to 3.3 x 106 Actinomycetes CFU/g from the plant Echinocactus grusonii, while AMF spores displayed the most elevated number up to 47 spore/25g from the plant Deuterocohnia sp. The percentage of AMF root infection is covered by 27,9% median. AMF identification was based on spore. The result showed an abundance presentation of Actinomycetes in each soil sample. AMF and Actinomycetes existence displayed symbiotic interaction between succulent plants and soil microorganisms. AMF and actinomycetes play the role of endophytes that help the growth of cacti, generally have a dry growing environment, and limited nutrition by this symbiotic interaction. Actinomycetes distribution in rhizospheric soil will increase plant growth-promoting factors activity. Eventually, the implications of research results are to explore the abundance and biodiversity of soil microorganisms from succulent plant rhizosphere that lacked exploration.

ANALISIS CEMARAN BAKTERI Coliform DAN Escherichia coli DARI SAMPEL ES BATU PADA PEDAGANG MINUMAN KAKI LIMA DI SEKITAR PASAR CIRACAS JAKARTA TIMUR MENGGUNAKAN METODE MOST PROBABLE NUMBER (MPN) Dwitami, Ananda Rizki; Achmadi, Achmadi; Wijayanti, Dian Rachma
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 2 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue2page194-201

Background: Water is a basic need of living things. Water is a liquid, when it freezes it turns into ice cubes. People often use ice cubes as a drink additive to give a fresh taste. The process of freezing ice cubes cannot destroy bacteria, many bacteria can live for a long time at low temperatures. This study aims to determine the contamination of Coliform and Escherichia coli bacteria in ice cubes sold by street vendors around Ciracas Market, East Jakarta. Methods: The MPN method is used to determine the range of Coliform bacteria in ice cube samples. E.coli contamination was detected using Eosine Methylene Blue Agar media. This study uses primary data by collecting samples and testing them in the microbiology laboratory. The sampling technique by purposive sampling. The samples used were ice cubes from street vendors around Ciracas Market is 8 samples. Results: 8 positive samples of Coliform bacteria and 5 positive samples of E.coli bacteria. In Sample 5 it has the highest value of 2900 MPN/100 mL. Sample 6 has the lowest value, namely 140 MPN/100 mL. Conclusion: The eight samples exceeded the contamination threshold allowed by Permenkes 416/Men.Kes/Per/IX/1990, that is 0/100 mL. In the EMBA media there were 5 positive samples of E.coli bacteria.

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