Claim Missing Document
Check
Articles

Penurunan Kadar Tembaga (Cu) dengan Biostimulasi Glukosa pada Bakteri Indigenous dari Sungai Bedera Kecamatan Medan Marelan, Kota Medan, Sumatera Utara Lubis, Resti Ayunda; Nasution, Rizki Amelia; Widiarti, Leni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.18072

Abstract

Sungai Bedera adalah sungai yang tergolong tercemar logam berat akibat pembuangan limbah rumah tangga dan industri yang memiliki kadar logam berat tembaga (Cu) yang tinggi yaitu 0,6518 mg/L. Cara yang dilakukan untuk menurunkan kadar logam berat tembaga (Cu) adalah dengan memanfaatkan bakteri Indigenous. Penelitian ini bertujuan untuk mengidentifikasi bakteri indigenous dari Sungai Bedera dan menguji kemampuannya dalam menurunkan kadar Cu. Bakteri Indigenous diberi perlakuan dengan nutrisi tambahan yaitu biostimulan glukosa. Metode yang dilakukan yaitu isolasi bakteri indigenous, uji resistensi bakteri dengan menghitung nilai Optical Density (OD) menggunakan spektrofotometer Uv-Vis dan proses penurunan kadar logam berat (Cu) dilakukan dengan metode SSA (Spektrofotometer Serapan Atom). Hasil uji menggunakan One Way ANOVA menunjukkan adanya perbedaan nyata antarperlakuan (Sig. < 0,05). Empat genus bakteri indigenous yang berhasil diidentifikasi memiliki kemampuan menurunkan kadar logam berat tembaga (Cu) yaitu Enterococcus, Bacillus, Corynebacterium, dan Staphylococcus. Persentase penurunan Cu tanpa biostimulan glukosa pada masing-masing bakteri Enterococcus, Bacillus, Corynebacterium dan Staphylococcus adalah 51,49%, 40,65%, 29,39%, dan 20,70%. Persentase penurunan Cu dengan penambahan biostimulan glukosa pada masing-masing bakteri Bacillus, Enterococcus, Staphylococcus dan Corynebacterium adalah (77,60%), (72,28%), (66,66%), dan (60,63%). Bakteri indigenous yang paling tinggi dalam menurunkan kadar tembaga (Cu) dengan penambahan biostimulan glukosa adalah Bacillus dengan persentase penurunan 77,60% dan bakteri indigenous yang paling rendah dalam menurunkan kadar tembaga (Cu) tanpa penambahan biostimulan glukosa adalah Staphylococcus dengan persentase penurunan 20,70%. Kata kunci : Bakteri Indigenous, Biostimulan, Logam Berat Tembaga (Cu), dan Sungai Bedera
Effectiveness of a nasal spray containing essential oil from Citrus microcarpa Bunge on the SEM profile of Aspergillus fumigatus mycelium Ananda, Revian; Mayasari, Ulfayani; Nasution, Rizki amelia
Bioma : Berkala Ilmiah Biologi Volume 28 Issue 1 Year 2026 (Issue in Progress)
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2026.78255

Abstract

Deteriorating air quality during seasonal transitions contributes to the increased growth of pathogenic microorganisms, including Aspergillus fumigatus, which may lead to adverse health impacts. Synthetic antifungal agents may induce resistance and undesirable side effects, thereby promoting interest in safer natural alternatives. This study aimed to evaluate the antifungal effectiveness of a nasal spray formulated with kasturi orange (Citrus microcarpa) peel essential oil against A. fumigatus. The essential oil was extracted through steam distillation and tested at five concentrations to identify the most effective range, after which three concentrations exhibiting the strongest antifungal activity were formulated into a nasal spray. Antifungal effectiveness was assessed using the disc diffusion method, morphological alterations of fungal hyphae were observed using Scanning Electron Microscopy (SEM), and statistical analysis was performed using Analysis of Variance (ANOVA). The results showed that the nasal spray demonstrated significant antifungal activity (p < 0.05), with inhibition zone diameters of 12.8 mm, 16.1 mm, and 20.5 mm at essential oil concentrations of 10%, 20%, and 30%, respectively. Although the highest concentration produced the largest inhibition zone, excessively high essential oil levels do not necessarily improve antifungal performance and may trigger adaptive responses approaching resistance. SEM observations confirmed notable cellular damage, including hyphal lysis, cell wall thinning, and structural shrinkage following exposure to the nasal spray formulation. In conclusion, kasturi orange peel essential oil is effective when formulated as a nasal spray to inhibit A. fumigatus, with 20% identified as the most suitable concentration for further product development.
REVIEW KERAGAMAN DAN STRUKTUR KOMUNITAS PROTISTA PADA PERAIRAN SALIN DAN HIPERSALIN BERDASARKAN FAKTOR SALINITAS DAN PH Adinugraha, Fajar; Nasution, Rizki Amelia
JOURNAL OF SCIENCE AND SOCIAL RESEARCH Vol 9, No 1 (2026): February 2026
Publisher : Smart Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54314/jssr.v9i1.5777

Abstract

Abstract: Saline and hypersaline waters are extreme ecosystems with high environmental stress that affect the survival of aquatic organisms, including protists as key components of the food web. This article aims to review and synthesize the influence of major environmental factors, particularly salinity and pH, on protist diversity and community structure. This study employed a narrative review method on ten selected scientific articles published between 2003 and 2025, covering studies in various habitats such as estuaries, salt ponds, saline lakes, and mangrove sediments. The review results indicate that salinity acts as a primary environmental filter; increased salinity generally correlates with a decrease in species richness but promotes the dominance of specialized halophilic taxa. Furthermore, extreme pH has been shown to limit physiological processes and protist growth. The application of modern molecular methods, such as environmental DNA (eDNA) and metabarcoding, has successfully revealed cryptic diversity of benthic and heterotrophic protists that are difficult to detect through conventional morphological methods. This study concludes that the interaction between physicochemical parameters and habitat characteristics strongly determines the dynamics and composition of protist communities in extreme aquatic environments. Keywords: Protists, Salinity, pH, Hypersaline waters, Diversity, eDNA.. Abstrak: Perairan salin dan hipersalin merupakan ekosistem ekstrem dengan tekanan lingkungan tinggi yang memengaruhi kelangsungan hidup organisme akuatik, termasuk protista sebagai komponen kunci dalam rantai makanan. Artikel ini bertujuan untuk meninjau dan menyintesis pengaruh faktor lingkungan utama, khususnya salinitas dan pH, terhadap keragaman serta struktur komunitas protista. Penelitian ini menggunakan metode narrative review terhadap sepuluh artikel ilmiah terpilih yang dipublikasikan dalam rentang tahun 2003–2025, mencakup studi di berbagai habitat seperti estuari, tambak garam, danau asin, dan sedimen mangrove. Hasil review menunjukkan bahwa salinitas bertindak sebagai filter lingkungan utama; peningkatan salinitas umumnya berkorelasi dengan penurunan kekayaan spesies namun meningkatkan dominasi taksa halofilik yang terspesialisasi. Selain itu, pH ekstrem terbukti membatasi proses fisiologis dan pertumbuhan protista. Penerapan metode molekuler modern, seperti environmental DNA (eDNA) dan metabarcoding, berhasil mengungkap keragaman tersembunyi (cryptic diversity) dari kelompok protista bentik dan heterotrof yang sulit dideteksi melalui metode morfologi konvensional. Studi ini menyimpulkan bahwa interaksi antara parameter fisik-kimia dan karakteristik habitat sangat menentukan dinamika dan komposisi komunitas protista di lingkungan perairan ekstrem. Kata kunci: Protista, Salinitas, pH, Perairan Hipersalin, Keanekaragaman, eDNA.
Analisis Kombinasi Bakteri Lactobacillus lactis dan Lactobacillus bulgaricus terhadap Protein dan Lemak Keju Susu Kerbau (Bubalus bubalis) selama Masa Simpan Sari, Linda; Nasution, Rizki Amelia; Selvia, Irda Nila
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.702

Abstract

Buffalo milk (Bubalus bubalis) has a high protein and fat content and lower cholesterol than cow's milk, but this nutritional content causes a relatively short shelf life so that further processing is required, one of which is to make cheese. During the shelf life, the quality of cheese can decrease due to microbial activity, enzymatic reactions, and protein and fat degradation. Lactobacillus lactis and Lactobacillus bulgaricus bacteria produce proteolytic enzymes that can modify the protein and fat content in cheese. This study aims to analyze the effect of the combination of Lactobacillus lactis and Lactobacillus bulgaricus bacteria on the protein and fat content of buffalo milk cheese at different shelf lives. The study used four treatments without repetition with shelf lives of 1, 7, and 14 days. Protein content was analyzed using the Kjeldahl method and fat content using the Soxhlet method. Data on pH, water content, total LAB, protein content, and fat content were analyzed descriptively quantitatively, while organoleptic tests were analyzed using the Kruskal–Wallis test followed by the Mann–Whitney test (p<0.05). The results showed that the combination of bacteria increased protein and fat content as the shelf life increased, with the highest values ??being 47.44% and 32.94%, respectively, accompanied by a decrease in pH and total LAB. In conclusion, the addition of 3% Lactobacillus lactis and 3% Lactobacillus bulgaricus can improve the quality of buffalo milk cheese. This research contributes to the science of food fermentation regarding the role of the combination of Lactobacillus lactis and Lactobacillus bularicus bacteria on the chemical characteristics of buffalo milk cheese during shelf life.
Analisis Kualitas Yogurt Santan Kelapa Dengan Penambahan Tepung Kolang Kaling Selama Penyimpanan Nasution, Rizki Amelia; Tambunan, Efrida Pima Sari
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1298

Abstract

The addition of flour from kolang kaling in this research aims to act as a stabilizer and improve the quality of fermented drinks (yoghurt) made from coconut milk. This research aims to analyze the quality of coconut milk yogurt with the addition of kolang kaling flour by testing its physicochemical properties. Experimental research with coconut milk yogurt samples with the addition of 1% kolang kaling flour and without the addition was stored at 40C for 14 days and analyzed for pH, viscosity and total LAB, as well as testing the research samples for water content using the distillation method (thermovolumetric), protein using the method Kjeldahl, fat using Soxhlet extraction, carbohydrate (starch) content testing using the UV Visible spectrophotometric method, and total acid using the titration and organoleptic method with 9 hedonic scales. Research shows that the results of adding palm fruit flour have an effect on the analysis parameters and are in accordance with SNI, namely pH reaches 5-3.94, carbohydrates decrease during H14 fermentation by 20.1%, protein reaches 6.5%, fat around 3%, water content is very low, namely 49%, The viscosity is very low, namely 2.8cP, the acid number is sufficient according to SNI in H14, namely 2%, %, as well as the hedonic results during storage which show statistical test results which are significantly different (P<0.05) on day 7 and day 14. The research can be concluded based on physicochemical analysis and conformity with SNI that the quality of coconut milk yogurt with the addition of kolang kaling flour during storage is good and in accordance with food standards.
Digitalizing Green Fashion Design via Service-Learning to Empower Fashion SMEs Nasution, Rizki Amelia; Tarigan, M. Faisal Afiff; Mirza, Mohammed Aquil
Jurnal IPTEK Bagi Masyarakat Vol 5 No 2 (2025)
Publisher : Ali Institute of Research and Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55537/j-ibm.v5i2.1369

Abstract

This community service research introduces a model of digitalizing green fashion through a service-learning approach implemented at Rumah Jahit Nila, a fashion MSME in Medan. The intervention includes intensive training on 3D design software such as CLO 3D and Marvelous Designer, the use of AI-assisted tools like Repsketch for pattern optimization, and production mentoring throughout a 12-month project cycle. Measurements show an increase in students' average technical competency scores from 55.1 to 76.9, representing a gain of 21.8 points. Partner evaluation was conducted through satisfaction questionnaires and field observations, both of which indicated positive acceptance of digital patterns and user guides. Claims regarding waste reduction and material efficiency are currently indicative, as direct quantitative metrics such as percentage reduction in fabric use or paper pattern sheets are not yet available; thus, further measurement is recommended. This study offers a replicable digital technology-based community service model for empowering sustainable fashion MSMEs.