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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Biologi Udayana Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kajian Bali Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Ethnobiology International Journal of Applied Science and Sustainable Development (IJASSD) International Journal of Scientific Multidisciplinary Research (IJSMR)
I Ketut Junitha
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Social Sciences

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Journal : SIMBIOSIS

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THE IMPACT OF LAUNDRY DETERGENT AS A METHOD OF WASHING TOWARDS SPERM DNA EXTRACTION RESULT ON COTTON FABRIC Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.185 KB) | DOI: 10.24843/JSIMBIOSIS.2021.v10.i01.p01

Abstract

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.
KUANTIFIKASI DNA PADA MAHASISWA PEROKOK DAN BUKAN PEROKOK DI UNIVERSITAS NEGERI MEDAN KECAMATAN MEDAN TEMBUNG KOTA MEDAN PROVINSI SUMATERA UTARA Kezia Artanauli Purba; I Ketut Junitha; Ni Nyoman Wirasiti
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.162 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p05

Abstract

Individual identification is very important in forensics. DNA can be obtained from all parts of the body with the same profile in everyone. Oral mucosal epithelium is one of the sources of DNA that is often used to examine individuals because it is taken using a harmless swab method. Smoke is one of the behaviors that are mostly done by young people or teenagers. Cigarette smoke affects the cells of the oral mucosa because it is a free radical. Free radicals are reactive oxygen compounds which are compounds with unpaired electrons. Deoxyribonucleic acid (DNA) is a nucleic acid polymer that is systematically arranged and is a carrier of genetic information that is passed on to offspring. This study aims to determine the quality and quantity of DNA in non-smoking and smoking students at the State University of Medan, Medan Tembung District, Medan City, North Sumatra Province. Samples were taken by swab method, namely mucosal epithelium from 60 probands consisting of 30 smoker proband and 30 non- smoking proband aged 18-22. The cheek in the probandus is swabbed from the back to the front in one direction. In this study, DNA extraction was carried out using a chelex solution which aims to separate DNA from protein, test the quantity of DNA with a spectrophotometer and test the quality of DNA with agarose gel. The results showed that the average DNA quantity in the oral mucosal samples was 1.96 ng/µL in the smokers proband and 6.92 ng/µL in the non-smoker proband and. The results of the quality test using electrophoresis on agarose gel showed that smoking students had thin bands of fluorescence and in some samples no bands of fluorescence at all. Meanwhile, in the probandus of non-smoker students, there were several samples that showed thin bands of luminescence and stains.
FORENSIC IDENTIFICATION BASED ON PRIMARY AND SECONDARY XAMINATION AS DETERMINATION OF VICTIMS IDENTIFIERS: NORTH LUWU FLOOD CASE STUDY, SOUTH SULAWESI Anastesya Hartika Nur Saputri; I Ketut Junitha; Ida Bagus Made Suaskara
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p01

Abstract

Forensic identification in mass disasters is carried out to determine the identity of the deceased. The identification process is important to provide psychological peace to the family with the certainty of the identity of the victim. Forensic identification methods are divided into primary examination (fingerprints, teeth, DNA) and secondary examination (medical characteristics and properties). This case study aims to determine the identity of flash flood victims in North Luwu, South Sulawesi based on primary and secondary examinations, determine the effect of the duration of the discovery on identification, and determine the further examination of unidentified victims. The research was carried out at the South Sulawesi Police Biddokes DVI post (RS. Hikmah Masamba) on 16-23 July 2020 and DNA examination was carried out at the Cell and Molecular Biology Laboratory, Faculty of Medicine, Udayana University accompanied by ethical clearance in June-August 2021. The material in In this study was the victims of the flash flood on the North Luwu, there were 40 victims, consisting of 31 victims who had been declared identified, 6 victims were missing or have not been found, and 3 victims had not been identified. Victims who are declared identified are based on the results of the examination (post mortem) which are compared with ante mortem data in accordance with the identification principle of Interpol (International Criminal Police Organization). Thirty-one victims identified were subjected to primary and/or secondary examination, of which 20 victims were identified by secondary examination and 11 victims were identified by primary and secondary examination, and 3 victims who had not been identified were conducted to DNA primary examination. Based on the results of DNA examination, it was found that the suspect's family was most likely a relative with one of the victims with a PM number 008.
Co-Authors A.A. Gde Lanang Adelina Arifiani Purnomo Agriani Dewinta Anastesya Hartika Nur Saputri Ayu Saka Laksmita W Bagas Susilo Bagus Alit, Ida Bambang Suryobroto Bram Setyadji Budiningsih, Desak Nyoman Cakra Diarsa Chandra Yowani Christian Subagya Gunardi Desak Nyoman Budiningsih Dewa Ayu Sri Ratnani Dewa Ayu Sri Ratnani Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Eswaryanthi Kusuma Yuni Fita Efi Rafelina Siallagan Graciella Stevani Gulo Gusti Ayu Putri Arnila Hanas, Dicky Frengky I Gde Suryadi I Gede Yeyen Suharta I Gusti Agung Eka Saraswati I K. Sukardika I Ketut Sundra I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Wayan Arya Mahardika Ida Ayu Manik Damayanti Ida Bagus Alit Ida Bagus Bajing Agastya Ida Bagus Made Suaskara IMA Gelgel Wirasutha Inna Narayani IRIANI SETYAWATI Kezia Artanauli Purba Lucia Emy Octavia MADE PHARMAWATI Maya Agustina Maya Agustina Meitini Proborini Wahyuni Muhamad Masyrur NI KADEK YUNITA SARI Ni Luh Putu Ria Puspitha Ni Luh Putu Rika Sugiantari Ni Luh Watiniasih Ni Made Puspawati Ni Made Rai Suarni Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri, Ni Putu Puniari Eka Ni Putu Putri Wulandari Putri Arie Prasetyoningrum Putu Rosi Setyari SANG KETUT SUDIRGA Septiasari, Ni Putu Senshi Tyara Tyara Victor Peter Poli Yan Ramona Yossy Carolina Carolina YOSSY CAROLINA UNADI Yulia Mirayanti