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All Journal e-GIGI Indonesian Journal of Cancer Chemoprevention IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) The Indonesian Biomedical Journal Narra J Indonesian Journal of Pharmaceutical Education Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu
Halim, Johni
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Uji Aktivitas Antioksidan Ekstrak Daun Amaranthus hybridus L. dalam Pelarut Heksana, Etil Asetat, dan Air Halim, Johni; Ranggaini, Monica D.; Tedjokusumo, Sabrina N.
e-GiGi Vol. 13 No. 1 (2025): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v13i1.56111

Abstract

Abstract: Noncommunicable diseases, such diabetes mellitus, atherosclerosis and cancer, caused by inter alia imbalance between free radicals and antioxidants within the body, known as oxidative stress. Antioxidants from outside the body are required as antioxidants within the body are not enough to ward off excessive free radicals. Amaranthus hybridus L. plant is quite popular among Indonesian people due to its fast harvest and advantages for health, including its natural antioxidants potency. This study aimed to determine the comparison of antioxidant activities among n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. leaves. This was an experimental laboratory study. Amaranthus hybridus L. simplicia extracted with maceration in n-hexane, ethyl acetate and aquadest. Antioxidant activities of n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. were examined with 2.2-diphenyl-1-picrylhydrazyl (DPPH) in 517 nm wavelength by using microplate reader. The results showed that the IC50 values of A. hybridus L. n-hexane, ethyl acetate, and aqueous extract respectively were 131.81 mg/mL, 103.53 mg/mL, and 79.61 mg/mL. In conclusion, there are differences in antioxidant activities between n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. This occur due to differences in the polarity of each solvent. Keywords: Amaranthus hybridus L.; antioxidant activities; maceration; solvent polarity    Abstrak: Penyakit tidak menular, seperti diabetes melitus, aterosklerosis, dan kanker, disebabkan antara lain oleh ketidakseimbangan antara radikal bebas dan antioksidan dalam tubuh (stres oksidatif). Antioksidan dari luar tubuh diperlukan karena antioksidan dari dalam tubuh tidak cukup untuk menangkal radikal bebas berlebih. Tanaman Amaranthus hybridus L. cukup diminati masyarakat Indonesia karena waktu panen yang singkat dan manfaatnya bagi kesehatan, termasuk potensi antioksidan alami. Penelitian ini bertujuan untuk mengetahui perbedaan aktivitas antioksidan ekstrak heksana, etil asetat, dan air daun A. hybridus L. Jenis penelitian ini berupa studi eksperimental laboratorik. Simplisia A. hybridus L. diekstraksi dengan metode maserasi dalam pelarut heksana, etil asetat, dan air. Aktivitas antioksidan ekstrak heksana, etil asetat, dan air daun A. hybridus L. diuji aktivitas antioksidan dengan metode 2,2-difenil-1-pikrilhidrazil (DPPH) pada panjang gelombang 517 nm dengan menggunakan microplate reader. Hasil penelitian menunjukkan nilai IC50 ekstrak heksana, etil asetat, dan air daun A. hybridus L. secara berturut-turut sebesar 131,81 mg/mL, 103,53 mg/mL, dan 79,61 mg/mL. Simpulan penelitian ini ialah terdapat perbedaan aktivitas antioksidan antara ekstrak heksana, etil asetat, dan air daun Amaranthus hybridus L. Hal ini terjadi karena perbedaan polaritas dari masing-masing pelarut. Kata kunci: Amaranthus hybridus L.; aktivitas antioksidan; maserasi; polaritas pelarut
Efektivitas Antibakteri Nanokomposit AgNP Cymbopogon citratus dengan Penstabil Kitosan terhadap Streptococcus viridans Halim, Johni; Siahaya, Aleceandra A. S.; Komariah, Komariah; Ranggaini, Dewi; Anggraeni, Rezky
e-GiGi Vol. 14 No. 1 (2026): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v14i1.64572

Abstract

Abstract: Nanocomposite is a synthesis of silver nanoparticles (AgNP) with lemongrass leaves (Cymbopogon citratus) stabilized with chitosan. The combination of nanocomposites has synergy in inhibiting bacteria, one of which is Streptococcus viridans that causes abscesses in the oral cavity. This study aimed to determine the effectiveness of nanocomposite chitosan AgCh with C. citratus in inhibiting the growth of S. viridans. This was an in vitro experimental laboratory study with a post-test group control design using disc diffusion for antibacterial test. Samples were divided into eight groups, with several concentrations 6.25 mg/mL, 12.5 mg/mL, 15 mg/mL, 25 mg/mL, and 50 mg/mL, positive control (clindamycin), and negative control (aquadest and acetic acid). Normality test showed a p-value of >0.05, which meant data were normally distributed. One-way ANOVA test showed a p-value <0.05, indicating there was a difference in each treatment. Therefore, further testing was carried out using the Tukey's post hoc. In conclusion, chitosan nanocomposite, AgCh, with C. citratus can inhibit the growth of S. viridans with the formation of the best inhibition zone at a concentration of 50 mg/mL, followed by concentrations (25, 12.5, 15, and 6.25) mg/mL. Keywords: nanocomposite; silver nanoparticles; Cymbopogon citratus; chitosan; Streptococcus viridans; abscess    Abstrak: Nanokomposit ialah sintesis nanopartikel perak (AgNP) dengan daun serai dapur (Cymbopogon citratus) yang distabilkan dengan kitosan. Gabungan nanokomposit memiliki sinergitas dalam menghambat pertumbuhan bakteri, salah satunya Streptococcus viridans yang menyebabkan abses dalam rongga mulut. Penelitian ini bertujuan untuk mengetahui efektivitas nanokomposit kitosan AgCh dengan C. citratus dalam menghambat pertumbuhan S. viridans. Jenis penelitian ialah eksperimental laboratorik in vitro dengan post test group control design. Pengujian antibakteri menggunakan metode difusi cakram. Sampel dibagi dalam delapan kelompok, masing-masing pada konsentrasi 6,25 mg/mL, 12,5 mg/mL, 15 mg/mL, 25 mg/mL, 50 mg/mL, kontrol positif (clindamycin), dan kontrol negatif (akuades dan asam asetat). Hasil uji normalitas menunjukkan p>0,05 yang berarti data terdistribusi normal. Uji oneway ANOVA menunjukkan nilai p<0,05 yang berarti terdapat perbedaan pada setiap perlakuan, sehingga dilakukan uji lanjut dengan menggunakan post hoc tukey. Simpulan penelitian ini ialah nanokomposit kitosan, AgCh, dengan C. citratus dapat menghambat pertumbuhan S. viridans dengan pembentukan zona hambat terbaik pada konsentrasi 50 mg/mL, diikuti oleh konsentrasi (25, 12,5, 15, dan 6,25) mg/mL. Kata kunci: nanokomposit; nanopartikel perak; Cymbopogon citratus; kitosan; Streptococcus viridans; abses
Co-Authors Alfred Pakpahan, Alfred Andrian Nova Fitri Anggraeni, Rezky Ariesanti, Yessy Aurelia Tjoe, Michelle Azrianty, Annisa Boedi Oetomo Roeslan, Boedi Oetomo Denilson Dewi Ranggaini Dhyani Widhianingsih Eddy Eddy Ferry Sandra Hartono, Tiffany Hayuningtyas, Ria A. I Gusti Ayu Ratih Utari Mayun Ichwan, Solachuddin Jauhari Arief Ihsan Rizal, Muhammad Jackson Jackson, Jackson Komariah Komariah Lee, Kyung H. Lee, Kyung Hoon Louisa, Marie Luki Astuti Melanie Sadono Djamil, Melanie Sadono Melyda, Ananda Shelly Nugroho, Didi Pratama Sarwono, Aditya Pratitis, Visi Endah Ranggaini, Dewi Ranggaini, Monica D. Ranggaini, Monica Dewi Scania, Alifah Evi Siahaya, Aleceandra A. S. Soesanto, Sheila Taramalinda, Elizabeth Yuliani Tedjokusumo, Sabrina N. Trisfilha, Pretty Yuliasari, Hani