Article Per Year (5 Year)
p-Index From 2021 - 2026
2.012
P-Index
This Author published in this journals
All Journal e-GIGI Indonesian Journal of Cancer Chemoprevention IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) The Indonesian Biomedical Journal Narra J Indonesian Journal of Pharmaceutical Education Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu
Halim, Johni
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
Efektivitas Antibakteri Nanokomposit AgNP Cymbopogon citratus dengan Penstabil Kitosan terhadap Streptococcus viridans Halim, Johni; Siahaya, Aleceandra A. S.; Komariah, Komariah; Ranggaini, Dewi; Anggraeni, Rezky
e-GiGi Vol. 14 No. 1 (2026): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v14i1.64572

Abstract

Abstract: Nanocomposite is a synthesis of silver nanoparticles (AgNP) with lemongrass leaves (Cymbopogon citratus) stabilized with chitosan. The combination of nanocomposites has synergy in inhibiting bacteria, one of which is Streptococcus viridans that causes abscesses in the oral cavity. This study aimed to determine the effectiveness of nanocomposite chitosan AgCh with C. citratus in inhibiting the growth of S. viridans. This was an in vitro experimental laboratory study with a post-test group control design using disc diffusion for antibacterial test. Samples were divided into eight groups, with several concentrations 6.25 mg/mL, 12.5 mg/mL, 15 mg/mL, 25 mg/mL, and 50 mg/mL, positive control (clindamycin), and negative control (aquadest and acetic acid). Normality test showed a p-value of >0.05, which meant data were normally distributed. One-way ANOVA test showed a p-value <0.05, indicating there was a difference in each treatment. Therefore, further testing was carried out using the Tukey's post hoc. In conclusion, chitosan nanocomposite, AgCh, with C. citratus can inhibit the growth of S. viridans with the formation of the best inhibition zone at a concentration of 50 mg/mL, followed by concentrations (25, 12.5, 15, and 6.25) mg/mL. Keywords: nanocomposite; silver nanoparticles; Cymbopogon citratus; chitosan; Streptococcus viridans; abscess    Abstrak: Nanokomposit ialah sintesis nanopartikel perak (AgNP) dengan daun serai dapur (Cymbopogon citratus) yang distabilkan dengan kitosan. Gabungan nanokomposit memiliki sinergitas dalam menghambat pertumbuhan bakteri, salah satunya Streptococcus viridans yang menyebabkan abses dalam rongga mulut. Penelitian ini bertujuan untuk mengetahui efektivitas nanokomposit kitosan AgCh dengan C. citratus dalam menghambat pertumbuhan S. viridans. Jenis penelitian ialah eksperimental laboratorik in vitro dengan post test group control design. Pengujian antibakteri menggunakan metode difusi cakram. Sampel dibagi dalam delapan kelompok, masing-masing pada konsentrasi 6,25 mg/mL, 12,5 mg/mL, 15 mg/mL, 25 mg/mL, 50 mg/mL, kontrol positif (clindamycin), dan kontrol negatif (akuades dan asam asetat). Hasil uji normalitas menunjukkan p>0,05 yang berarti data terdistribusi normal. Uji oneway ANOVA menunjukkan nilai p<0,05 yang berarti terdapat perbedaan pada setiap perlakuan, sehingga dilakukan uji lanjut dengan menggunakan post hoc tukey. Simpulan penelitian ini ialah nanokomposit kitosan, AgCh, dengan C. citratus dapat menghambat pertumbuhan S. viridans dengan pembentukan zona hambat terbaik pada konsentrasi 50 mg/mL, diikuti oleh konsentrasi (25, 12,5, 15, dan 6,25) mg/mL. Kata kunci: nanokomposit; nanopartikel perak; Cymbopogon citratus; kitosan; Streptococcus viridans; abses
Aktivitas Antioksidan dengan Metode DPPH dan ABTS Terhadap Ekstrak Etanol Daun Amaranthus hybridus L. Dewi Ranggaini, Monica; Halim, Johni; Aurelia Tjoe, Michelle
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20891

Abstract

Background: Cancer, a degenerative disease, arises from free radicals in the body, which can be counteracted by antioxidants. Amaranthus hybridus L., a vegetable rich in antioxidant compounds like flavonoids, requires extraction to harness these compounds. This extraction uses the maceration technique with 70% ethanol. Antioxidant activity is tested using methods like DPPH and ABTS assays, with the IC50 value indicating effectiveness. Purpose: To determine the difference in antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods. Method: Laboratory experimental research. Simplicia leaves of A. hybridus L. in 70% ethanol solvent were extracted using maseration method. The antioxidant activity was tested in the sample and the ascorbic acid using DPPH and ABTS methods at wavelenghts 517 nm and 745 nm. Sample absorbance was measured using microplate reader. Result: IC50 values of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods were 54,99 µg/mL and 71,45 µg/mL, for ascorbic acid using DPPH and ABTS methods were 0,62 µg/mL and 3.13 µg/mL. Conclusion: The antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH method has stronger antioxidant than ABTS method.
Co-Authors Alfred Pakpahan, Alfred Andrian Nova Fitri Anggraeni, Rezky Ariesanti, Yessy Aurelia Tjoe, Michelle Azrianty, Annisa Boedi Oetomo Roeslan, Boedi Oetomo Denilson Dewi Ranggaini Dhyani Widhianingsih Eddy Eddy Ferry Sandra Hartono, Tiffany Hayuningtyas, Ria A. I Gusti Ayu Ratih Utari Mayun Ichwan, Solachuddin Jauhari Arief Ihsan Rizal, Muhammad Jackson Jackson, Jackson Komariah Komariah Lee, Kyung H. Lee, Kyung Hoon Louisa, Marie Luki Astuti Melanie Sadono Djamil, Melanie Sadono Melyda, Ananda Shelly Nugroho, Didi Pratama Sarwono, Aditya Ranggaini, Dewi Ranggaini, Monica D. Ranggaini, Monica Dewi Scania, Alifah Evi Siahaya, Aleceandra A. S. Soesanto, Sheila Taramalinda, Elizabeth Yuliani Tedjokusumo, Sabrina N. Trisfilha, Pretty Yuliasari, Hani