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All Journal e-GIGI Indonesian Journal of Cancer Chemoprevention The Indonesian Biomedical Journal Narra J Indonesian Journal of Pharmaceutical Education Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu
Halim, Johni
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Aktivitas Antioksidan dengan Metode DPPH dan ABTS Terhadap Ekstrak Etanol Daun Amaranthus hybridus L. Dewi Ranggaini, Monica; Halim, Johni; Aurelia Tjoe, Michelle
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20891

Abstract

Background: Cancer, a degenerative disease, arises from free radicals in the body, which can be counteracted by antioxidants. Amaranthus hybridus L., a vegetable rich in antioxidant compounds like flavonoids, requires extraction to harness these compounds. This extraction uses the maceration technique with 70% ethanol. Antioxidant activity is tested using methods like DPPH and ABTS assays, with the IC50 value indicating effectiveness. Purpose: To determine the difference in antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods. Method: Laboratory experimental research. Simplicia leaves of A. hybridus L. in 70% ethanol solvent were extracted using maseration method. The antioxidant activity was tested in the sample and the ascorbic acid using DPPH and ABTS methods at wavelenghts 517 nm and 745 nm. Sample absorbance was measured using microplate reader. Result: IC50 values of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods were 54,99 µg/mL and 71,45 µg/mL, for ascorbic acid using DPPH and ABTS methods were 0,62 µg/mL and 3.13 µg/mL. Conclusion: The antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH method has stronger antioxidant than ABTS method.
Co-Authors Andrian Nova Fitri Anggraeni, Rezky Ariesanti, Yessy Aurelia Tjoe, Michelle Azrianty, Annisa Boedi Oetomo Roeslan, Boedi Oetomo Denilson Dewi Ranggaini Dhyani Widhianingsih Eddy Eddy Ferry Sandra Hartono, Tiffany Hayuningtyas, Ria A. I Gusti Ayu Ratih Utari Mayun Ichwan, Solachuddin Jauhari Arief Ihsan Rizal, Muhammad Jackson Jackson, Jackson Komariah Komariah Lee, Kyung H. Lee, Kyung Hoon Louisa, Marie Luki Astuti Melanie Sadono Djamil, Melanie Sadono Nugroho, Didi Pratama Sarwono, Aditya Ranggaini, Dewi Ranggaini, Monica D. Ranggaini, Monica Dewi Scania, Alifah Evi Siahaya, Aleceandra A. S. Soesanto, Sheila Taramalinda, Elizabeth Yuliani Tedjokusumo, Sabrina N. Trisfilha, Pretty Yuliasari, Hani