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All Journal Jurnal Ilmu Lingkungan Jurnal Biologi Tropis Jurnal Ilmiah Keperawatan (Scientific Journal of Nursing) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Kumawula: Jurnal Pengabdian Kepada Masyarakat Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ners Community Empowerment Poltekita: Jurnal Pengabdian Masyarakat Bioscientist : Jurnal Ilmiah Biologi Jurnal Abdiraja TRANSFORMASI : JURNAL PENGABDIAN PADA MASYARAKAT Jurnal Keperawatan dan Kesehatan (JKK) Jurnal Pengabdian Harapan Ibu (JPHI) Jurnal Abdimas Kesehatan
Khairillah, Yuyun Nisaul
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Journal : Bioscientist : Jurnal Ilmiah Biologi

 1 
Isolation and Identification of Bacteria Producing Antibiotic Compounds from The Rhizosphere of Avicennia Marina Against Pathogenic Bacteria in The Mangrove Ecosystem Area of West Kalimantan Khairillah, Yuyun Nisaul; Alang, Hasria; Haryanto, Haryanto; Fitriagustiani, Fitriagustiani; Erwhani, Indri; Purnamawati, Ditha Astuti; Rahmawati, Annisa; Surtikanti, Surtikanti; Triwahyuni, Adelia
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12527

Abstract

Antibiotic resistance is a global problem in the health sector. The level of antibiotic resistance that occurs can be caused by several influencing factors, including 6 isolates of rhizosphere bacteria that were successfully isolated from the rhizosphere of the Avicenia marina plant. The purpose of this study was to isolate and determine the best minimum inhibitory concentration of rhizosphere bacteria of the A. marina plant and its effectiveness in inhibiting pathogenic bacteria S. aureus. This study is a quantitative research method using the isolation and characterization of rhizosphere bacteria, antagonistic test of rhizosphere bacteria and test of the results of the extraction of filtrate of rhizosphere bacteria culture of A. marina plants. The results of this study were (1) five of the isolates of bacteria that were successfully isolated were included in the Bacilus type and 1 isolate of bacteria included in the Cocobasil type; (2) there were 3 of the 6 isolates that could inhibit the growth of pathogenic bacteria S. aureus with an inhibition range of 10.04-4.42 mm; (3) Rhizosphere bacterial isolate (TN 1) is an isolate with the best inhibition diameter and is categorized as strong, namely 10.04 mm; (4) ethyl acetate extract of Rhizosphere bacterial isolate culture filtrate (TN 1), has a minimum inhibitory concentration of 0.50% against pathogenic bacteria S.aureus with an inhibition diameter of 7.18 mm which is better when compared to the positive control using chloramphenicol.
Isolation and Identification of Bacteria Producing Antibiotic Compounds from The Rhizosphere of Avicennia Marina Against Pathogenic Bacteria in The Mangrove Ecosystem Area of West Kalimantan Khairillah, Yuyun Nisaul; Alang, Hasria; Haryanto, Haryanto; Fitriagustiani, Fitriagustiani; Erwhani, Indri; Purnamawati, Ditha Astuti; Rahmawati, Annisa; Surtikanti, Surtikanti; Triwahyuni, Adelia
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12527

Abstract

Antibiotic resistance is a global problem in the health sector. The level of antibiotic resistance that occurs can be caused by several influencing factors, including 6 isolates of rhizosphere bacteria that were successfully isolated from the rhizosphere of the Avicenia marina plant. The purpose of this study was to isolate and determine the best minimum inhibitory concentration of rhizosphere bacteria of the A. marina plant and its effectiveness in inhibiting pathogenic bacteria S. aureus. This study is a quantitative research method using the isolation and characterization of rhizosphere bacteria, antagonistic test of rhizosphere bacteria and test of the results of the extraction of filtrate of rhizosphere bacteria culture of A. marina plants. The results of this study were (1) five of the isolates of bacteria that were successfully isolated were included in the Bacilus type and 1 isolate of bacteria included in the Cocobasil type; (2) there were 3 of the 6 isolates that could inhibit the growth of pathogenic bacteria S. aureus with an inhibition range of 10.04-4.42 mm; (3) Rhizosphere bacterial isolate (TN 1) is an isolate with the best inhibition diameter and is categorized as strong, namely 10.04 mm; (4) ethyl acetate extract of Rhizosphere bacterial isolate culture filtrate (TN 1), has a minimum inhibitory concentration of 0.50% against pathogenic bacteria S.aureus with an inhibition diameter of 7.18 mm which is better when compared to the positive control using chloramphenicol.
Diversity and Potential Active Compound of the Sponge-Associated Bacteria from Lemukutan Island, West Kalimantan Indonesia as New Type of Antibiotics Baihaqi, M. Fikri; Nabila, Faika; Triwahyuni, Adelia; Fahira, Annisa Nasywa; Khairillah, Yuyun Nisaul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14660

Abstract

This study aims to determine the diversity of sponge symbiont bacteria and the effectiveness of active compounds of bacteria that are symbiotic with marine sponges from Lemukutan Island waters. The methods in this study are isolation of sponge symbiont bacteria, characterization of sponge symbiont bacteria, antagonistic tests, activity tests of the best sponge symbiont bacterial isolate extract culture filtrate, and GCMS/MS tests. The results showed that nineteen isolates of sponge symbiont bacteria were successfully isolated from sponge types from Theonella cylindrica and Hyattella intestinalis sponges in Lemukutan waters. Seven isolates of sponge symbiont bacteria are included in the Gram-positive bacteria genre. While the other twelve are included in Gram-negative bacteria. Ten of the nineteen isolates of sponge symbiont bacteria have antibacterial activity for S. aureus. Isolate Sp4 10-6 B, based on the results of antagonistic tests, has an inhibition diameter of 10.28 mm, which is categorized as solid inhibition. Ethyl acetate extract of Sp4 10-6 culture filtrate has a minimum inhibitory concentration value of 0.5% against pathogenic bacteria S.aureus. The active compound with the potential for antibiotic development is the Tetradecane compound, which has the highest peak area of the eight other types of compounds. This shows that the concentration of the compound contained is higher than the other compounds. This compound is included in the aliphatic hydrocarbon and alkane groups.
Co-Authors Annisa Rahmawati Ayu Diana Meilantika Baihaqi, M. Fikri Baihaqi, M. Fiqri Baihaqi, Muh. Fiqri Djaswadi Dasuki Fahira, Annisa Nasywa Fitri Handayani Fitriagustiani, Fitriagustiani Fitriana, Erlis Eka Haryanto Haryanto Hasria Alang Husaini Husaini Indri Erwhani Jagad, Nur Jati Kardiatun, Tutur Khoirillah, Fanni Mardiyani, Ridha Mentari, Tiara Amalia Mumtahanah, Al Nabila, Faika Nasir, Agung Nuruniyah, Nuruniyah Pasmawati, Pasmawati Pratama, Kharisma Pratama, Sandi Fransisco Purnamawati, Ditha Astuti Putri, Anggraini Putri, Nurul Febriani Putri, Sartika Gunawan Ramanda, Galih Dwiki riwayati riwayati Sri Ariyanti Sukartina Suriadi Jais Surtikanti Surtikanti Sutikanti Syamsuri tri wahyuni Triwahyuni, Adelia Utami, Anggraini Putri