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The Effect of WPM (Woody Potent Medium) Dosage on the In Vitro Development of Milo Pace Variety Robusta Coffee Leaf Explants: Pengaruh Dosis Media WPM (Woody Pant Medium) Terhadap Perkembangan Eksplan Daun Kopi Robusta Varietas Milo Pace Secara In Vitro Az Zahra, Rana; Irsyadi, Muhammad Burhanuddin; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarok; Rosyadi, Muhammad Ghufron; Wulanjari, Distian; Farisi, Oria Alit
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.10823

Abstract

Milo pace robusta coffee is a local coffee variety in Jember Regency that is threatened with extinction. One way to overcome this problem is by propagating it through tissue culture. The success of tissue culture is determined by the use of optimal culture media doses. Research related to the development of Milo pace coffee is still very limited. This study aims to obtain the best dose in woody plant medium media for the development of robusta coffee leaf explants of the Milo pace variety. This study used a completely randomized design (CRD) with a single factor, namely the WPM media dose (1 time, ¾ times, ½ times and MS 1 time as a control) which was repeated 3 times with 3 samples. Data were analyzed descriptively and statistically using analysis of variance, and continued with the DMRT test at a 95% confidence level. The results showed that the use of WPM media with 1 dose was the best dose with a percentage of imbibed explants of 77.77% with an average emergence at 2.53 ± 0.68 DAI and green live explants. Furthermore, the percentage of browning explants was still high at 88.89±19.24%, which appeared at 9.96±1.73 DAI. Various doses of WPM media were unable to induce callus in Milo Pace robusta coffee leaf explants.
Biokonversi Sampah Organik Berbasis Maggot BSF dalam Pemberdayaan PKK RT 1 RW 7 di Kelurahan Sumbersari, Jember: Bioconversion of Organic Waste Using BSF Maggots in the Empowerment of PKK RT 1 RW 7 in Sumbersari Subdistrict, Jember Habriantono, Bakhroini; Irsyadi, Muhammad Burhanuddin; Suharto, Suharto; Wagiyana, Wagiyana; Alfarisy, Fariz Kustiawan; Nurlaelita, Khaerani; Dewi, Rifani Rusiana; Sari, Wahyu Intan; Latifa, Siti Nur; Ramadhan, Gilang; Fourthina, Amanda Intan; Setyawan, Rizki Agus
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. 4 (2026): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v11i4.11589

Abstract

Household organic waste is a major component of waste generation in densely populated residential areas, especially in urban areas with limited land and time. In RT 1 RW 7, Sumbersari Village, waste management is not optimally carried out, as the waste transportation system still relies on the door-to-door method, with no processing at the household level. This community service program aims to increase community awareness and involvement, especially among PKK mothers, in managing organic waste through bioconversion technology using Black Soldier Fly (BSF) maggots. The activity was carried out through a participatory approach in the form of a Focus Group Discussion (FGD) that was aligned with the PKK's regular meeting agenda. Residents were educated on how to use maggots, starting with sorting organic and non-organic waste. PKK mothers were also shown the decomposition process using BSF maggots firsthand, thereby convincing them of the program's benefits. Regular monitoring and evaluation were conducted to assess the program's effectiveness and changes in community behavior in sorting and processing waste. Monitoring results showed that most residents were quite enthusiastic about implementing the organic waste conversion program with maggots.