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All Journal Vegetalika JMM (Jurnal Masyarakat Mandiri) BIO-EDU: Jurnal Pendidikan Biologi Proceedings Series on Physical & Formal Sciences Literasi: Jurnal Pengabdian Masyarakat dan Inovasi Journal of Soilscape and Agriculture Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Nasional (JPN) Indonesia PAPUMA: Journal of Comunnity Services Proceeding of International Conference on Biology Education, Natural Science, and Technology
Irsyadi, Muhammad Burhanuddin
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Education Electrical & Electronics Engineering Engineering Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Physics Social Sciences Other

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The Effect of WPM (Woody Potent Medium) Dosage on the In Vitro Development of Milo Pace Variety Robusta Coffee Leaf Explants: Pengaruh Dosis Media WPM (Woody Pant Medium) Terhadap Perkembangan Eksplan Daun Kopi Robusta Varietas Milo Pace Secara In Vitro Az Zahra, Rana; Irsyadi, Muhammad Burhanuddin; Kusbianto, Dwi Erwin; Suud, Hasbi Mubarok; Rosyadi, Muhammad Ghufron; Wulanjari, Distian; Farisi, Oria Alit
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.10823

Abstract

Milo pace robusta coffee is a local coffee variety in Jember Regency that is threatened with extinction. One way to overcome this problem is by propagating it through tissue culture. The success of tissue culture is determined by the use of optimal culture media doses. Research related to the development of Milo pace coffee is still very limited. This study aims to obtain the best dose in woody plant medium media for the development of robusta coffee leaf explants of the Milo pace variety. This study used a completely randomized design (CRD) with a single factor, namely the WPM media dose (1 time, ¾ times, ½ times and MS 1 time as a control) which was repeated 3 times with 3 samples. Data were analyzed descriptively and statistically using analysis of variance, and continued with the DMRT test at a 95% confidence level. The results showed that the use of WPM media with 1 dose was the best dose with a percentage of imbibed explants of 77.77% with an average emergence at 2.53 ± 0.68 DAI and green live explants. Furthermore, the percentage of browning explants was still high at 88.89±19.24%, which appeared at 9.96±1.73 DAI. Various doses of WPM media were unable to induce callus in Milo Pace robusta coffee leaf explants.
Co-Authors Abyan, Anfirta Kauthar Akbar, Dimas Raihan Al Fahmi, Amrullah Alawiyah, Riffa Leshia Muhvi Nur Alit Farisi, Oria Anom Wijaya, Ketut Aris, Irfan Ayuningtias, Nandini Az Zahra, Rana Az-Zahra, Rana Dewi, Naomi Dias Laksita Dwi Erwin Kusbianto Farisi, Oria Alit Febrianti, Amellia Rizki Gatot Subroto Hak, Moh Alaika Nurul Handayani, Etty Hasanah, Rizky Hasbi Mubarok Suud, Hasbi Mubarok Hutami, Trapsila Siwi Innaka Ageng Rineksane Izah, Ika Lailatul Kamilah, Arina Dian KHOIRUL ANAM Maulana, Rafli Akbar Mubaroq, Amirul Khamdhan Octavia, Nurrul Asma'ul Permatasari, Fany Puspito, Agung Nugroho Rafsanjani, Muhammad Akbar Ratnawati Ratnawati Risma, Lucky Ardina Rosyadi, Muhammad Ghufron Rosyady, Muhammad Ghufron Safitri, Eka Dian Setiyono, S Sri Rejeki Dwi Astuti Wulanjari, Distian Wulanjari, Distiana Zahrosa, Dimas Bastara