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All Journal UNEJ e-Proceeding Nusantara Medical Science Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Ilmu Alam dan Lingkungan Indonesian Journal of Obstetrics & Gynecology Science Alami Journal (Alauddin Islamic Medical) Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Biomedika Jurnal Riset Kesehatan Poltekkes Depkes Bandung Celebes Health Journal Indian Journal of Forensic Medicine & Toxicology JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Majalah Anestesia & Critical Care (MACC)
Firdaus Hamid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : UNEJ e-Proceeding

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IDENTIFICATION OF DERMATOPHYTES BY MULTIPLEX-POLYMERASE CHAIN REACTION, POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ITS1-ITS4 PRIMERS AND MVAI, AND POLYMERASE CHAIN REACTION (GACA)4 PRIMER Rizalinda Sjahril; Firdaus Hamid; Aan Yulianingsih; Novita Prastiwi; Awaluddin Awaluddin; Siska Nuryanti; Faridha Ilyas; Burhanuddin Bahar
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Laboratory identification of skin lesion is important for the correct diagnosis and choice of therapy. Microscopic examination of skin or nail scraping or hair fragments in 10%-KOH provides rapid result but fungal growth in culture is required for identification of species. Unfortunately, culture requires a few days to 2 weeks, and there is variable colony appearance and colour. Rapid and correct diagnosis has been enabled by Polymerase Chain Reaction (PCR), but has not yet been applied for routine diagnosis of patients. Therefore we investigated the ability of culture using Saboraud Dextrose Agar, multiplex-PCR, PCR-RFLP with ITS1-ITS4 primers and MvaI, and PCR with (GACA)4 primer to identify of the etiology agents of 130 patients with tinea who were positive showing hyphae in 10%-KOH preparation. Skin scrapings were collected in Makassar during January-June 2016 and examinations were carried out in the Microbiology Laboratory of Hasanuddin University. Results: Dermatophytosis occurred in 73 (56,1%) males, and 57 (43,8%) females. Scraping was obtained from 78 (60%) skin and 52 (40%) nail lesions. Based on age stratification, 68 (52,3%) were 10-18 years old, 43 (33%) were 19-45 years old, and 19 (14,6%) were >45 years old. While 39 (30%) samples grew in culture, Multiplex-PCR, PCR-RFLP with ITS1-ITS4 primers and MvaI, and PCR with GACA4 primer amplified DNA of 130 (100%), 126 (96,9%), and 106 (81,5 %) samples, respectively. Multiplex-PCR was not able to distinguish between spesies in 99 (76,2%), PCR-RFLP with ITS1-ITS4 primers and MvaI in 29 (22,3%) and PCR with GACA4 primer in 20 (15,4%) samples.
Co-Authors A. Kaisar Adiwijaya Putra A.M. Takdir Musba Aan Yulianingsih Aan Yulianingsih Anwar Ade Rifka Junita Agussalim Bukhari Ahyar Ahmad Ahyar Ahmad Alamsyah Ambo Ala Husain Alfian Zainuddin Alfina Baharuddin Aminuddin Aminuddin Andi Alfian Zainuddin Andi Alfian Zainuddin Andi Asadul Islam Andi Ihwan Andi Meutiah Ilhamjaya Andi Nursanty Padjalangi Andi Rofian Sultan Andi Zsazsa Rafiatul Mukhlis Arif Santoso Arif Santoso Arsyi Adliah Anwar Awaluddin Awaluddin Baedah Madjid Besse Afriana Arif Bukhari, Agussalim Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Dario A Nelwan Dianawaty Amiruddin Eddy Hartono Eddyman W Ferial Efendi Lukas Elizabeth Catherine Jusuf Erwin Chandra Evi Damayanti Faridha Ilyas Fatmawati Annisa Syamsuddin Febyan Rasmin Kotto Fonny Josh Hardjono Sumarlie Hursepuny, Valentine Irawati Djaharuddin Irawaty Djaharuddin Junus Asiu Bulu Baan Kenny Edward Yap Khairuddin Djawad Lisa Tenriesa M. Sabir Madonna Damayanthie Datu Misriyani Misriyani Mochammad Hatta Mochammad Hatta Mochammad Hatta Muh. Nasrum Massi Muh. Ramli Ahmad Muhammad Nassrum Massi Muhammad Reza Primaguna Mustika Hutabarat Sari Mustika Sari Hutabarat Nasrudin Andi Mappeware Nita Mariana Noor Ramadhaniah Novita Prastiwi Nur Indah Purnamasari Nur Surya Wirawan Nusratuddin Abdullah Priady Wira Prasetia Ressy Dwiyanti Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rosdiana Natzir Rossana Agus Sachraswaty Rachman Samrichard Rambulangi Sharvianty Arifuddin Siska Nuryanti Siti Fitrah Irmiyanti Hamid Solly Aryza Sri Wahyu Sri Wahyuni Sulmiati Sulmiati Suryani As’ad Syahruni Syahrir TOKA, WAHYUNITA DO Veni Hadju Wahyuni Alinengsih Yoeke Dewi Rasita Yulfa Rizki Amita