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Aktivitas Antibakteri Membran Nanokomposit Kitosan/Nanopartikel Perak Notriawan, Doni; Nesbah, Nesbah; Ernis, Gustria; Fadhila, Muhammad Adeng; Wibowo, Risky Hadi; Pertiwi, Reza; Ilfanisari, Vinolla
ALCHEMY Vol 9, No 1 (2021): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v9i1.11146

Abstract

The aim of this study was to make and test the antibacterial activity of chitosan/silver nanoparticles nanocomposite membranes. Nanocomposite membranes were synthesized by chitosan with pluronic using acetic acid as a solvent. Silver nanoparticles were synthesized using the green synthesis method and the makasar fruit (Brucea javanica L. Merr) peel extract as a bioreductor. The silver nanoparticles were composited with a chitosan/pluronic mixture and printed on a glass plate. Nanocomposite membranes were characterized using the FTIR spectrophotometer and the scanning electron microscope (SEM). Nanocomposite membrane were tested for antibacterial activity against Escherechia coli. The UV-Vis spectra showed the formation of silver nanoparticles which were indicated by the absorption at 454 nm and the absorbance value of 0.405. Characterization using FTIR showed no new functional groups formed in the composites of chitosan and pluronic. SEM results showed the difference between the chitosan membrane and the nanocomposite membrane. The surface of nanocomposite membrane showed uneven compared to the chitosan membrane. Nanocomposite membranes have antibacterial activity to inhibit E. coli growth.   Keywords: nanocomposite, chitosan/silver nanoparticles, antibacterial Penelitian ini bertujuan untuk membuat dan menguji aktivitas antibakteri membran nanokomposit kitosan/nanopartikel perak. Membran nanokomposit dibuat dengan mensintesis kitosan dengan pluronik menggunakan pelarut asam asetat. Nanopartikel perak disintesis menggunakan metode green synthesis dengan ekstrak kulit buah makasar (Brucea javanica L. Merr) sebagai bioreduktor. Nanopartikel perak dikompositkan dengan campuran kitosan/pluronik dan dicetak di atas plat kaca. Membran nanokomposit dikarakterisasi menggunakan spektrofotometer FTIR dan scanning electron microscope (SEM). Membran nanokomposit dilakukan uji aktivitas antibakteri terhadap Escherechia coli. Spektra UV-Vis menunjukkan terbentuknya nanopartikel perak yang ditandai adanya serapan pada panjang gelombang 454 nm dan absorbansi 0,405. Karakterisasi dengan FTIR menunjukkan tidak adanya gugus fungsi baru yang terbentuk pada komposit dari kitosan dan pluronik. Hasil SEM menunjukkan adanya perbedaan antara membran kitosan dengan membran nanokomposit. Permukaan membran nanokomposit terlihat tidak rata dibandingkan membran kitosan. Membran nanokomposit memiliki aktivitas bakteri sehingga dapat mengambat pertumbuhan bakteri E. coli. Kata kunci: nanokomposit, kitosan/nanopartikel perak, antibakteri
The effect of Raphanus sativus and Pachyrhizus erosus juice combination on the ethanol-induced gastric of mice Reza Pertiwi; Aanisah Hanuun; Noval Kurniawati; Petri Siti Khodijah; Dian Fita Lestari; Dyah Fitriani; Doni Notriawan
Pharmaciana Vol 11, No 3 (2021): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.078 KB) | DOI: 10.12928/pharmaciana.v11i3.20104

Abstract

Gastric ulcers can be caused by active inflammation due to impaired mucosal integrity, which causes local or widespread lesions. Gastric ulcers can occur due to the use of ethanol, which has a local effect on the gastric. Therefore, the continuous consumption of ethanol can damage gastric cells. Raphanus sativus and Pachyrhizus erosus contain flavonoids, which are well-known to reduce stomach acid as a curative agent.  The objective of this study is to see the effect of Raphanus sativus and Pachyrhizus erosus juice as a preventive agent on alcohol-induced gastric ulcers in mice (Mus musculus). A combination of Raphanus sativus and Pachyrhizus erosus was given at a dose of 100, 300, and 600 mg/kgBW. Specifically, Raphanus sativus was given at a dose of 300 mg/kgBW, Pachyrhizus erosus at a dose of 300 mg/kgBW, and positive control was included using sucralfate. The treatment was carried out for 12 days, and 1 hour after the last day of treatment, 20 ml/kgBW of ethanol induction was given 24 hours after the animals were dissected. The results showed that the index value of gastric ulcers in the normal group, negative control, and the combination of Raphanus sativus and Pachyrhizus erosus juice given orally at a dose of 100, 300, and 600 mg/kgBW (Raphanus sativus at a dose of 300 mg/kgBW, Pachyrhizus erosus at a dose of 300 mg/kgBW, and sucralfate as positive control) were 0; 4.00; 1.00; 1.33; 2.33; 2.67; 0 and 2.00. The results of the histopathological analysis also showed improvement in the gastric of mice fed with Pachyrhizus erosus juice. Therefore, it is inferred that treatment using Raphanus sativus and Pachyrhizus erosus juice can reduce the number of ulcers, increase ratio protection, and repair the cells in gastric histopathology. Additionally, giving a single dose of Pachyrhizus erosus juice results in a more significant gastric improvement. 
Penentuan Status Mutu Air Tanah Dangkal di Sekitar Tempat Pembuangan Akhir Sampah (TPA) Air Sebakul Kota Bengkulu menggunakan Metode STORET: A Cross-Sectional Study Deni Agus Triawan; Doni Notriawan; Gustria Ernis
Jurnal Kimia Riset Vol. 5 No. 1 (2020): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v5i1.17053

Abstract

Ground Water quality has a special significance for drinking, hygiene sanitation and domestic water supply. The study aims to understand the distribution of ground water quality in solid waste landfill of Bengkulu City. The following objectives of the study are to determine ground water quality parameters such as pH, total dissolved solid, electrical conductivity, hardness, iron, manganese, nitrate and nitrite. Sampling was carried out at a radius of 0 - 250 meters from the location of the landfill. The results of the pH at 10 sampling points show the values that are quite varied between 6,45 – 7,59; conductivity between 56 µS – 492 µS; TDS between 28 mg/L – 246 mg/L; salinity 32 mg/L – 284 mg/L; Iron (Fe) 0,040 mg/L – 0,323 mg/L; Manganese (Mn) 0,089 mg/L – 0,295 mg/L; Nitrate (NO3-) 0,2 mg/L – 0,8 mg/L and Nitrite (NO2-) 0,006 mg/L – 0,033 mg/L. Based on an assessment with the STORET system, water quality in the study area is classified as good (score =-4) by parameters such as temperature, pH, total dissolved solid, electrical conductivity, hardness, iron, manganese, nitrate and nitrite. By the total sampling sites, all sample site had good-quality water for hygiene sanitation based on STORET method.
Aktivitas Antibakteri Nanopartikel Perak Hasil Green Synthesis Menggunakan Ektrak Kulit Buah Kemuning (Murraya Paniculata (L) Jack) Doni Notriawan; Gustria Ernis; Risky Hadi Wibowo; Reza Pertiwi; Tessa Rianti Malau
BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Vol 3 No 2 (2020): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.997 KB) | DOI: 10.31539/bioedusains.v3i2.1850

Abstract

Antibacterial activity using silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract has been carried out. This study aims to determine the antibacterial activity of green synthesized silver nanoparticles using Murraya Paniculata (L) rinds extract. Silver nanoparticles were synthesized by reacting AgNO3 with water extract of the Murraya Paniculata (L) rinds. Green synthesized silver nanoparticles were tested for antibacterial activity using Escherichia coli ATCC 8739. Silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract were characterized using a UV-Vis spectrophotometer to confirm the formation of silver nanoparticles. The UV-Vis spectrophotometer results showed the formation of silver nanoparticles which was indicated by an absorption around 400-450 nm. The antibacterial activity test results of silver nanoparticles showed a strong inhibitory activity. Keywords: Green Synthesis, Silver Nanoparticles, Antibacterial, Echerichia Coli
Uji In Vitro Aktivitas Imunomodulator Minyak Atsiri Serai Dapur (Cymbopogon citratus) terhadap Proliferasi Sel Limfosit Mencit Gustria Ernis; Doni Notriawan; Dyah Fitriani; Elvira Yunita; Inta Cantika
BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Vol 4 No 2 (2021): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.527 KB) | DOI: 10.31539/bioedusains.v4i2.2524

Abstract

This study aims to determine the immunomodulatory activity of lemongrass essential oil on the proliferation of mouse lymphocyte cells in vitro. The method used is steam distillation to obtain citronella essential oil. Isolation of lymphocyte cells was obtained from the spleen organ of Swiss-Webster strain mice which was carried out aseptically. The lymphocyte cell proliferation test was carried out using the MTT Assay method and the absorbance was measured using an ELISA reader at 550 nm. The results showed that the lemongrass essential oil contained positive alkaloids, flavonoids, saponins, and steroids/triterpenoids with an essential oil yield of 0.043%. The higher the volatile oil concentration, the higher the Optical Density (OD) value obtained, but the OD increase at a 50 to 100 g/mL concentration was not significantly different. In conclusion, lemongrass essential oil has immunomodulatory activity against lymphocyte cell proliferation in mice tested in vitro. Keywords: Immunomodulator, Essential Oil, Lemongrass (Cymbopogon citratus), Immune System
Pembinaan Sistem Pengelolaan Sampah Terpadu Berbasis 3R (Reduce, Reuse, Recycle) Kepada Mayarakat Desa Rindu Hati Bengkulu Tengah Doni Notriawan; Muhammad Adeng Fadila; Fades Br Gultom; Donna Sagita Mala; Sella Adesafitri; Olin Rafflesia
Surya Abdimas Vol. 6 No. 1 (2022)
Publisher : Universitas Muhammadiyah Purworejo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37729/abdimas.v6i1.1515

Abstract

Kebersihan merupakan salah satu faktor penting dalam suatu desa wisata. Berdasarkan hasil analisa dilapangan, Desa Wisata Rindu Hati belum memiliki tata kelola sampah yang baik. Pengabdian ini bertujuan untuk memberikan pengtahuan kepada masyarakat Desa Rindu Hati tentang pengelolaan sampah secara 3R sehingga dapat dimanfaatkan kembali. Kegiatan pengabdian Masyarakat ini terdiri dari 3 tahapan yaitu tahapan persiapan, tahapan pelaksanaan, dan monitoring. Pada tahapan persiapan kegiatan pengabdian diawali dengan melakukan koordinasi dengan kepala desaRindu Hati. Koordinasi dilakukan bertujuan untuk menyampaikan tentang kegiatan yang akan dilaksanakan. Kegiatan dilaksanakan pada tanggal 31 Agustus 2021 dengan cara ceramah, tanya jawab, dan praktik tentang pengelolaan sampah secara 3R kepada warga desa Rindu Hati. Berdasarkan hasil angket evaluasi pemahaman warga terhadap materi didapatkan hasil peningkatan pemahaman warga tentang pengelolaan sampah secara 3R. Kegiatan ini perlu keberlanjutan dengan melakukan sosialisasi terhadap pengunjung desa Wisata Rindu Hati.
Pengaruh Pemberian Perasan Lobak Putih (Raphanus sativus L.) dan Bengkuang (Pachyrhizus erosus L.) terhadap Kadar Glukosa Darah pada Mencit Model Diabetes Reza Pertiwi; Petri Siti Khodijah; Mifta Violina Aniza; Noval Kurniawati; Eni Kurniati; Dian Handayani; Dian Fita Lestari; Doni Notriawan
Jurnal Farmasi Sains dan Terapan Vol 9, No 1 (2022): Februari
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v9i1.3027

Abstract

Kadar glukosa darah adalah jumlah kandungan glukosa yang ada pada plasma darah. Pengukuran kadar glukosa darah puasa merupakan salah satu metode untuk mengidentifikasi penyakit diabetes melitus pada seseorang. Bengkuang memiliki serat larut air yang dapat memperlambat absorpsi glukosa sehingga dapat menurunkan kadar glukosa darah. Lobak putih memiliki kesamaan jenis dengan bengkuang yaitu merupakan jenis tanaman umbi yang diharapkan memiliki efek yang sama dalam menurunkan kadar glukosa darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian perasan lobak putih dan bengkuang terhadap kadar glukosa darah mencit yang diinduksi aloksan sebagai model diabetes. Mencit dibagi menjadi 8 kelompok dengan pembagian secara random dan dipuasakan selama 8 jam, pada hari ke–0 diukur kadar glukosa darahnya sebagai kadar glukosa darah awal. Kelompok I sebagai kontrol normal yang tidak diberi induksi aloksan dan diberi aquades. Pada kelompok II-VI, mencit diinduksi aloksan dengan dosis 200 mg/kg BB secara intraperitoneal. Setelah aloksan diinduksikan, diukur kadar glukosa darah mencit pada hari ke-3. Setelah didapati kadar glukosa darah mencit yang tinggi, kelompok mencit II diberi aquades, kelompok III diberi glibenklamid 0,013 mg/20 gBB mencit, kelompok IV diberi perasan lobak putih 0,1 ml/20 gBB, kelompok V diberi perasan lobak putih dan bengkuang 2:1, kelompok VI diberi perasan lobak putih dan bengkuang 1:1, kelompok VII diberi perasan lobak putih dan bengkuang 1:2, kelompok VIII diberi perasan bengkuang 0,1 ml/20 gBB. Pemberian perasan lobak putih dan bengkuang dapat menurunkan kadar glukosa darah.
Pemanfaatan Tanaman Obat Keluarga (TOGA) Meningkatkan Imunitas Tubuh sebagai Pencegahan COVID-19 Reza Pertiwi; Doni Notriawan; Risky Hadi Wibowo
DHARMA RAFLESIA Vol 18, No 2 (2020): DESEMBER (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v18i2.12665

Abstract

Pada bulan Desember, 2019, serangkaian kasus pneumonia yang penyebabnya tidak diketahui muncul di Wuhan, Hubei, Cina, dengan gambaran klinis sangat menyerupai virus pneumonia. Setelah dilakukan uji laboratorium menunjukkan adanya virus baru, yang diberi nama COVID-19. Jumlah kasus terus tumbuh secara eksponensial menyebabkan WHO mengingatkan semua negara untuk melakukan kegiatan pencegahan dan pengendalian yang kuat agar dapat menghentikan penyebaran virus ini. Sejauh ini, belum ditemukannya obat spesifik untuk penyembuhan infeksi COVID-19. Terapi yang digunakan hanyalah menghilangkan gejala yang muncul dengan meningkatkan imunitas penderita. Potensi peningkatan imunitas dari jamu dapat diperoleh dari tanaman obat keluarga (TOGA). Tanaman obat dapat meningkatkan kekebalan tubuh, karena bersifat pencegahan (preventif) dan promotif melalui kandungan metabolit sekunder contohnya seperti gingiro pada jahe dan santoriso pada temulawak yang mampu meningkatkan sistem kekebalan tubuh. Berdasarkan observasi mahasiswa UNIB dan masyarakat di Kelurahan Pematang Gubernur, Kecamatan Muara Bangkahulu RT. 22, masih membutuhkan jamu sebagai pencegahan terhadap penyebaran virus COVID-19
In Vitro Immunomodulatory Activity of Virgin Coconut Oil (VCO) with and without Bromelain Enzyme from Pineapple Waste (Ananas comosus (L) Merr) Gustria Ernis; Devianri M; Nurul Afiatun Hasanah; Dyah Fitriani; Doni Notriawan; Deni Agus Triawan
Jurnal Kimia Sains dan Aplikasi Vol 25, No 4 (2022): Volume 25 Issue 4 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2511.971 KB) | DOI: 10.14710/jksa.25.4.155-160

Abstract

Immunomodulators are an essential part of the prevention process for treating various diseases related to the body’s immune system. This study aimed to determine the immunomodulatory activity of virgin coconut oil (VCO) with and without the enzyme bromelain in pineapple waste extract on the proliferation of mice lymphocyte cells through an in vitro test. VCO was made using two methods: enzymatic using bromelain enzyme (VCOb) from pineapple waste with 10, 25, and 50%, and mixing method without bromelain enzyme (VCOm). The two types of VCO produced were calculated for the yield, moisture content, free fatty acids (FFA), and physicochemical properties. For immunomodulatory activity, the test solution was taken from VCOb and VCOm with a variation concentration of 6.25, 12.5, 25, 50, and 100 µg/mL. Isolation of lymphocyte cells was obtained from the spleen organ of Swiss Webster strain mice which was performed aseptically with a ketamine-xylazine anesthetic. The lymphocyte cell proliferation test was performed using the MTT Assay, and the Optical Density was measured using a microplate reader at 550 nm. The results showed that 50% VCOb produced the highest yield and 22.22% higher than VCOm. However, the results showed that increasing bromelain enzyme concentration would increase the moisture content and free fatty acid content, but still below 0.2%. The VCOb and VCOm had immunomodulatory activity against mice lymphocyte cell proliferation. However, the immunomodulatory activity of VCO with bromelain enzyme from pineapple waste extract (VCOb) was higher than without bromelain enzyme (VCOm). The highest immunomodulatory activity was obtained at 100 µg/mL of VCOb with a percentage increase of 158.26% compared to negative controls, followed by VCOm of 100 µg/mL with a percentage increase of 137.66% compared to negative controls. The optimum dose of VCOm and VCOb for increasing the proliferation of mice lymphocyte cells has not been found.
Nutrition Levels of Liquid Organic Fertilizer from Onion Skin (Allium cepa. L) with EM-4 Bioactivator Gustria Ernis; Donna Sagita Mala; Amelisa Okta; Doni Notriawan; Muhammad Adeng Fadila
JURNAL SAINS NATURAL Vol. 13 No. 2 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i2.468

Abstract

The high production of shallots (Allium cepa. L) causes a large amount of shallot waste in the form of shallot skins, which are organic wastes with a light weight, easily dispersed, disturbing the landscape and polluting the environment as well as being a source of disease. So that it is necessary to process waste into materials that have useful and even economic value, one of which is Liquid Organic Fertilizer (POC). The purpose of this study was to determine the levels of macro and micro nutrients in POC with varying concentrations of EM-4 bioactivator. In this study, the manufacture of POC with various concentrations of bioactivator 0, 25, 50, and 100 mL with a fermentation time of 14 days. Then carried out physicochemical observations, namely observing physical and chemical changes ranging from viscosity, color, odor and the presence or absence of gas in the POC made. The resulting POC was then tested for macro-nutrients (N, P, K and C-organic) and micro-nutrients (Fe, Mn, and Cu) using AAS and UV-Vis spectrophotometer. In this study, the best POC of onion peel waste was obtained at a concentration of 100 mL bioactivator with macronutrients N, P, K, C-Organic, each of which was 0.71; 1.45; 0.36 and 4.7%; while the content of micronutrients Fe, Mn, and Cu were 0.037, respectively; 0.0052, and 0.198%. It can be concluded that the levels of macro and micro nutrients have complied with SNI 19-7030-2004 except for C-organic elements which are still far below the standard, and POC made from onion skin has a high phosphorus content, so it is very suitable for use in plants that have high levels of phosphorus. require growth regulators (ZPT), especially for root growth.