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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI)

. Giyanto

Department Of Plant Protection, Faculty Of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680

Author-ID : 209322

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Published : 26 Documents Claim Missing Document

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Identification of Quorum Quenching Bacteria and Its Biocontrol Potential Against Soft Rot Disease Bacteria, Dickeya dadantii Syaiful Khoiri; Tri Asmira Damayanti; Giyanto Giyanto
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i1.633

Dickeya dadantii is one of newly found bacteria causing soft rot on orchids in Indonesia. Infected plants showed severe rot rapidly only in few days. An effort to control the bacteria was conducted by utilizing selected quorum quenching (QQ) inducer bacteria which produce AHL-lactonase by aiiA gene. The aims of this research were to screen and identify of quorum quenching bacteria, and also assayed their biocontrol potential ability against D. dadantii in laboratory. The screening of QQ bacteria was achieved using the anti-QS test, anti-microbial activity, and detection of aiiA gene using specific primer. The determination of the ability against D. dadantii was done using the soft rot assay on potato and orchid. Among thirty one bacteria isolates screened, four isolates (in succession namely B37, BT2, GG3, and GG6) were selected to control D. dadantii. All of these bacteria showed QQ ability to suppress the virulence of D. dadantii infection on orchids, significantly. Based on nucleotide sequences of 16S ribosomal RNA, those of bacteria isolates had the highest identity with Brevibacillus brevis, Bacillus cereus ATCC14579, Bacillus cereus ATCC14579 and Bacillus thuringiensis ATCC 10792. Brevibacillus brevis was reported for the first time as QQ bacteria in this study.

Detection of Wolbachia Endosymbiont in Several Agriculturally Important Insect Parasitoids in Bogor, Indonesia Mahardika Gama Pradana; Giyanto Giyanto; Seiichi Furukawa; Satoshi Nakamura; Damayanti Buchori
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1855

Wolbachia that have been found to be maternally inherited in arthropods, including insects behave primarily as a reproductive parasite by inducing feminization and/or death among genetic males; causing cytoplasmic incompatibility, and activating parthenogenesis, in host insects. This research aims to detect the presence of Wolbachia in several parasitic insects and to determine the diversity of Wolbachia at the supergroup level. Insect genetic samples were collected, amplified using wsp (Wolbachia surface protein) gene and with the sequences then analyzed using species homologues drawn from the Genbank database. These activities were dedicated to detect intracellular presence of Wolbachia and assembled the phylogenetic tree of the respected bacteria. Phylogenetic analysis confirmed that Wolbachia existed in the samples belonged to the supergroup A (found in Cherapron sp.) and supergroup B (found in E. japonica). Wolbachia were detected in seven out of nine wasps i.e. Anagyrus lopezi, Ceraphron sp., Microplitis manilae, Brachymeria lasus, Scelionidae sp01, Trichogramma sp, and Exorista japonica. Single infection by Wolbachia supergroup A was detected in A. lopezi, Ceraphron sp., M. manilae, and Scelionidae sp01. Meanwhile single infection by Wolbachia supergroup B was detected only in E. japonica. Double infection by both supergroups occurred in B. lasus and Trichogramma sp. samples.

The Endophytic Bacteria, Salicylic Acid, and their Combination as Inducers of Rice Resistance Against Xanthomonas oryzae pv. oryzae Christoffol Leiwakabessy; Meity Suradji Sinaga; Kikin H. Mutaqien; Trikoesoemaningtyas Trikoesoemaningtyas; Giyanto Giyanto
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.1029

Bacterial leaf damage or blight brought by bacteria Xanthomonas oryzae pv. oryzae (X. oryzae pv. oryzae) is considered as an extremely serious disease of rice worldwide, including Indonesia. Induced resistance using chemical and biological agents was considered as a method to control the disease. The objectives of this research were to analyze of endophytic bacteria (Lysinibacillus sphaericus/L.sphaericus) and salicylic acid as the inducers of rice resistance against X. oryzae pv. oryzae. This study used three-factorial incompletely randomized designs to study the effect of endophytic bacteria and salicylic acid on three varieties of rice. The results of the study showed that endophytic bacteria and salicylic acid induced rice resistance to X. oryzae pv. oryzae. This endophytic bacterium was also able to increase the plant height (AUPHGC), the number of tillers (AUNTGC), and the number of productive tillers. The application of salicylic acid at the concentrations of 10 mM was able to suppress the BLB disease and increased the number of tillers and 1000-grain weight. The result of study showed that the endophytic bacteria and salicylic acid could induce resistance of rice varities against BLB disease and increased the number of productive tillers.

Efektivitas Ekstrak Tumbuhan untuk Mengeliminasi Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Zainal, Aprizal; Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, ,; Giyanto, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Objectives of experiments were to evaluate (1) in vitro inhibitory effects of plant extracts on Clavibacter michiganensis subsp. michiganensis (Cmm), (2) inhibitory effects of plant extracts on Cmm infected tomato seeds, and (3) effectiveness of seed treatment plus plant extracts to eliminate Cmm. After evaluating 20 plant extracts, curcuma rhizome and betel vine leaf extract, cinnamon and clove oil were selected for further test. Tomato seeds were artificially inoculated with Cmm to obtain high level of infection. Part of the seeds were dipped in either suspension of selected extracts for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 °C and RH 60-70%) plus either of plant extracts oils, respectively. Elimination of Cmm level from infected seeds was observed at 10 days after treatments. Results of the experiment indicated curcuma extract, betel vine extract, cinnamon oil or clove oil showed in vitro inhibitory effects on Cmm. Moreover, dipping infected seeds in either 5% of curcuma, betel vine extract, or 0.5% of clove oil or matriconditioning plus these extracts oils were effective to eliminate Cmm from infected seeds. These treatments may potentially be used and developed commercially for eliminating seedborne Cmm on infected tomato seeds. Keywords: Bacterial cancer, seedborne-pathogen, seed-treatments

Uji Inokulasi dan Respon Ketahanan 38 Genotipe To mat terhadap Clavibacter michiganensis subsp. michiganensis Zainal, Aprizal; Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, ,; Giyanto, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 2 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Identifi kasi ketahanan genotipe adalah langkah awal dalam pengembangan kultivar tahan terhadap serangan patogen. Tujuan penelitian ini adalah (i) mendapatkan cara inokulasi dengan jumlah dan konsentrasi inokulum Cmm yang efektif untuk mengevaluasi ketahanan tomat terhadap Cmm di rumah kaca, (ii) mendeterminasi reaksi ketahanan berbagai genotipe tomat akibat inokulasi Cmm. Percobaan ini menggunakan 38 genotipe tomat yang terdiri dari 7 genotipe tomat lokal, 15 genotipe tomat komersial, dan 16 genotipe koleksi Pusat Studi Pemuliaan Tanaman IPB Bogor (PSPT/IPB). Agen penyebab penyakit yang digunakan adalah 6 isolat Cmm hasil percobaan sebelumnya. Cara inokulasi Cmm yang efektif terhadap tomat cv. Marta (sangat rentan), uji reaksi ketahanan berbagai genotipe tomat terhadap Cmm telah dilakukan di rumah kaca. Kesimpulan dari penelitian ini adalah (i) inokulasi dengan menyuntikkan inokulum Cmm 5 µl konsentrasi 106 cfu/ml pada beberapa tempat di ketiak daun (daun pertama, daun tengah dan pucuk) merupakan cara yang paling efektif mengevaluasi ketahanan tomat terhadap Cmm, (ii) berbagai genotipe tomat yang diuji belum ada yang tahan teradap Cmm, genotipe tomat lokal ada yang agak rentan dan agak tahan.Kata kunci: kultivar tahan, suspensi bakteri, tomat

Perlakuan Benih untuk Meningkatkan Mutu dan Produksi Benih serta Mengendalikan Penyakit Bulai pada Jagung Manis Sonhaji, Muhammad Yasin; Surahman, Memen; Ilyas, Satriyas; ., Giyanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perlakuan benih untuk meningkatkan mutu dan produksi benihserta mengendalikan penyakit bulai pada jagung manis. Galur jagung manis 06 digunakan pada percobaan di rumah plastikyang disusun berdasarkan rancangan acak lengkap satu faktor, yaitu 13 perlakuan benih. Percobaan di lapangan disusunberdasarkan rancangan petak terbagi dengan dua faktor. Faktor pertama merupakan galur jagung yang terdiri atas duagalur yaitu galur 06 dan 07, dan faktor kedua merupakan perlakuan benih yang terdiri atas 7 perlakuan. Matriconditioningmenggunakan rasio benih : arang sekam : pelarut = 3:0.5:1 (g) dalam botol tertutup dan ditempatkan pada ruangan bersuhu20±2 C selama 24 jam. Fungisida sintetik yang digunakan mengandung metalaksil dan dimethomorf. Agen hayati yangdigunakan adalah Bacillus megaterium dan Brevibacillus laterosporus. Hasil percobaan di rumah plastik dan lapanganmenunjukkan bahwa perlakuan fungisida sintetik paling efektif menekan penyakit bulai. Percobaaan di lapangan menunjukkanbahwa galur 07 lebih tahan terhadap penyakit bulai dibanding galur 06. Perlakuan matriconditioning + fungisida sintetik+ B. laterosporus pada galur 07 menghasilkan bobot tongkol per tanaman paling tinggi. Perlakuan matriconditioning +fungisida sintetik + B. laterosporus pada galur 06 mampu meningkatkan mutu fisiologis benih hasil panen.Kata kunci: Bacillus megaterium, Brevibacillus laterosporus, matriconditioning, Peronosclerospora maydis

Co-Authors Abdjad Asih Nawangsih Agus Eko Prasetyo Aisyah Imas ALI NURMANSYAH Andi Takdir Anwar, Aswaldi Aprizal Zainal Aris Budiman Awaludin Hipi Bambang Nuryanto Dadang Dadang DAMAYANTI BUCHORI Dori Kusuma Jaya DWI ANDREAS SANTOSA Efi Toding Tondok Elvina Efendi Eny Widajati Erina Sulistiani Gustan Pari Gustan Pari Haliatur Rahma, Haliatur Herliyana Indahwardani Idham Sakti Harahap Imas Aisyah Indahwardani, Herliyana Kikin H Mutaqin Kikin H. Mutaqien Kresnamurti Kurniasih Leiwakabessy, Christoffol Mahardika Gama Pradana Meity S Sinaga, Meity S Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Suharman, Memen Memen Surahman Mokhammad Danang Kusuma Mubin, Nadzirum Muhammad Evan Nurrahmawan Muhammad Yasin Sonhaji Novik Nurhidayat RAHAYU WIDYASTUTI Ratna Sari Dewi Sanjaya, Wilhelmus Terang Arga Sarah Desmia Muchtar Sarah Desmia Muchtar Sarijan, Abdullah - Sarjiya Antonius Satoshi Nakamura SATRIYAS ILYAS Seiichi Furukawa Setiawan, Asep S Sudarsono Supramana Syaiful Khoiri TRI ASMIRA DAMAYANTI Trikoesoemaningtyas Tuminem ., Tuminem

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