Article Per Year (5 Year)
p-Index From 2020 - 2025
1.431
P-Index
This Author published in this journals
All Journal Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Jurnal Mahasiswa Farmasi Fakultas Kedokteran dan Ilmu Kesehatan UNTAN Jurnal Didaktik Matematika INDONESIAN JOURNAL OF PHARMACY Molecular and Cellular Biomedical Sciences (MCBS) JSFK (Jurnal Sains Farmasi & Klinis) JURNAL PENELITIAN JPK: Jurnal Proteksi Kesehatan Literacy: Jurnal Pendidikan Ekonomi Jurnal Vokasi Keperawatan (JVK) Andalasian Livestock Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Marlina .
Fakultas Farmasi Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 23 Documents
Search

 1   2   3 
Isolation of bacterial pathogen Escherichia coli o157:h7 in seafood samples and detection of flich7 gene by using PCR Marlina Marlina
Indonesian Journal of Pharmacy Vol 20 No 2, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp73-76

Abstract

Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, haemolytic-uraemic syndrom and thrombotic thrombocytopenic purpura.In this study, P. pelagicus dan P. merguensis samples were collected from markets in Padang from Sept 2007 until Feb 2008. For isolation of the bacteria, samples were firstly enriched in mEC media, followed by plating onto CHROMAgar 0157 for E.coli O157:H7. Consequently, the suspected colonies were confirmed by polymerase chain reaction (PCR) assay using primers specific for O157:H7 to detect a fliCH7 gene. Only 15 of them confirmed as E. coliO157:H7 in PCR assay.Key words: Escherichia coli O157:H7, CHROMAgar 0157, fliCH7 gene, PCR.
Isolation of bacterial pathogen Escherichia coli o157:h7 in seafood samples and detection of flich7 gene by using PCR Marlina .; Elidahanum Husni; Fitri Amalinda; Son Radu; Mitsuaki Nishibuchi
Indonesian Journal of Pharmacy Vol 20 No 2, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.715 KB) | DOI: 10.14499/indonesianjpharm0iss0pp73-76

Abstract

Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, haemolytic uraemic syndrom and thrombotic thrombocytopenic purpura.In this study, P. pelagicus dan P. merguensis samples were collected from markets in Padang from Sept 2007 until Feb 2008. For isolation of the bacteria, samples were firstly enriched in mEC media, followed by plating onto CHROMAgar 0157 for E.coli O157:H7. Consequently, the suspected colonies were confirmed by polymerase chain reaction (PCR) assay using primers specific for O157:H7 to detect a fliCH7 gene. Only 15 of them confirmed as E. coli O157:H7 in PCR assay.Key words: Escherichia coli O157:H7, CHROMAgar 0157, fliCH7 gene, PCR
Isolation, Characterization, Proliferation and Differentiation of Synovial Membrane-derived Mesenchymal Stem Cells (SM-MSCs) from Osteoarthritis Patients Marlina Marlina; Rizki Rahmadian; Armenia Armenia; Wahyu Widowati; Rizal Rizal; Hanna Sari Widya Kusuma; Satrio Haryo Benowo Wibowo; Wahyu Setia Widodo; Ika Adhani Sholihah
Molecular and Cellular Biomedical Sciences Vol 4, No 2 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i2.100

Abstract

Background: Mesenchymal stem cells (MSCs) are the cells which have high renewal capacity and and are capable for differentiating into some types of cells. MSCs can be obtained from several tissues including bone marrow, synovial membrane, blood, adipose tissue and periosteum. The proliferation and self-repair ability of MSCs are the advantages to use as stem cells-based therapy of various diseases. The aim of this study was to determine the differentiation, characterization and proliferation of synovial membrane-derived MSCs (SM-MSCs).Materials and Methods: The cells proliferation capacity was determined by cell counting using trypan blue, characterization of MSCs (cluster of differentiation (CD)90, CD11b, CD73, CD34, CD19, CD45, CD105 and human leukocyte antigen-DR isotype (HLA-DR)) using flow cytometry analysis, and differentiation capability into three lineage cells was determined with red alcian blue, oil red O and alizarin staining.Results: The type culture of SM-MSCs was adherent and showed positive CD44, CD105, CD73, CD90 and negative of CD19, HLA-DR, CD11b, CD45, CD34 surface marker. Based on the result, SM-MSCs P3 showed differentiation potency into adipogenic, chondrogenic, and osteogenic lineage cells. The population doubling time of SM-MSCs has increased from P3 to P8. The population doubling time of SM-MSCs P3 was 1.69 days and SM-MSCs P8 was 3.64 days.Conclusion: The results indicated that SM-MCSCs from osteoarthritis patients are able to differentiate into osteocytes, chondrocytes, adipocytes and highly express of CD105, CD73, CD90, CD44 and negative for CD34, CD45, CD14, CD19.Keywords: synovial membrane, mesenchymal stromal cells, adipocyte, chondrocyte, osteocyte
Identifikasi Type Human Papillomavirus (HPV) pada Penderita Kanker Serviks Marlina Marlina; Yufri Aldi; Andani Eka Putra; Densi Selpia Sopianti; Dewi Gulyla Hari; Arfiandi Arfiandi; Akmal Djamaan; Rustini Rustini
Jurnal Sains Farmasi & Klinis Vol 3, No 1 (2016): J Sains Farm Klin 3(1), November 2016
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.511 KB) | DOI: 10.29208/jsfk.2016.3.1.100

Abstract

Human Papillomavirus (HPV) is the most significant risk factor for the cause of cervical cancer. The purpose of this study for identification of HPV types 16, 18, 31, 33, 45 and 52 in cervical cancer patients. HPV is a row of high-risk HPV types that can cause cervical cancer. Total sample of 78 diisolat DNA derived from FFPE, cervical smears and cervical cancer fresh tissue obtained from Dr. Dr. M. Djamil, Padang and hospitals. Arifin Achmad, Pekanbaru. HPV DNA detection is done by using Polymerase Chain Reaction (PCR) using universal primers GP5 +/6 +. HPV types were identified by PCR with specific primers. Total sample types obtained with concentrations varying between 0.9 to 645 ng / ml with purity DNA in accordance with the specified purity for PCR amplification. The results of the study of 78 patients with cervical cancer samples, 42 samples (54%) identified HPV DNA. HPV type 18 is more dominant and followed by HPV type 16 as compared to the other types, namely the percentage of 40.4% and 28.5%. HPV type 45 (7.1%), HPV type 52 (2.3%) and HPV 31 and HPV type 33 was not detected.
Identifikasi Gen E7 Isolat Human Papillomavirus Tipe 18 (HPV18) dari Penderita Kanker Serviks Arfiandi Arfiandi; Densi Selpia Sopianti; Dewi Gulyla Hari; Marlina Marlina; Yufri Aldi; Andani Eka Putra; Akmal Djamaan; Rustini Rustini
Jurnal Sains Farmasi & Klinis Vol 3, No 1 (2016): J Sains Farm Klin 3(1), November 2016
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.463 KB) | DOI: 10.29208/jsfk.2016.3.1.95

Abstract

Gen E7 HPV18 merupakan salah satu onkoprotein yang selalu diekspresikan oleh HPV pada sel yang terinfeksi. Infeksi persisten oleh Human papillomavirus (HPV) beresiko tinggi adalah faktor etiologi utama untuk kanker serviks dan ekspresi Onkoprotein HPV E7 disarankan untuk menjadi penanda potensial untuk perkembangan tumor. E7 akan mempengaruhi aktivitas pRB supresor tumor serta mengikat dan mengaktifkan kompleks cyclin sehingga dapat menimbulkan kanker serviks. Dalam penelitian ini akan dilakukan identifikasi terhadap Gen E7 isolat HPV18 dari penderita kanker serviks yang berasal dari RSUP M. Jamil Padang, Sumatera Barat dan RSUD Arifin Ahmad, Pekan Baru, Riau. Proses identifikasi dilakukan dengan metode Polymerase Chain Reaction (PCR) menggunakan primer spesifik untuk gen E7 HPV18. Dari lima belas isolat HPV18 yang diamplifikasi dengan primer spesifik gen E7 HPV18, didapatkan sepuluh sampel positif teridentifikasi gen E7 (66,6%) sedangkan lima sampel lainnya tidak teridentifikasi. Infeksi kanker pada penderita kanker serviks yang berasal dari RSUP. M. Jamil, Padang dan RSUD. Arifin Ahmad, Pekan Baru, Riau sebagian besar disebabkan oleh gen E7 HPV18 sehingga hal ini bisa dijadikan penanda potensial untuk perkembangan tumor bagi penderita kanker serviks di Sumatera Barat dan Riau pada khususnya serta di Indonesia pada umumnya.
Pengaruh Pemberian Fibroblast Growth Factor (FGF) dari Telur Ayam Terfertilisasi Terhadap Kadar Glukosa Darah Mencit Hiperglikemia Irene Puspa Dewi; Surya Dharma; Marlina Marlina
Jurnal Sains Farmasi & Klinis Vol 3, No 1 (2016): J Sains Farm Klin 3(1), November 2016
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.583 KB) | DOI: 10.29208/jsfk.2016.3.1.86

Abstract

A study on the effect of Fibroblast Growth factor (FGF) derived from fertilized chicken egg on blood glucose level in hyperglycemia mice induced by alloxan has been done. FGF taken from fertilized chicken egg white, the egg white dried with Freeze dryer method and powdered into flour. The egg powdered was tested quantitatively by ELISA (The Enzimed-Link immunosorbent assay ) method to determine the levels of FGF contained therein. And then, FGF was given to hyperglycemia mice induced alloxan. Blood glucose levels were observed periodically every week for 4 weeks after administration of FGF preparations. The results found, the levels of FGF in fertilized chicken egg white powder contained about 219 ng / L and the induction of FGF to hyperglycemia mice were able to improve blood glucose levels in mice hyperglycemia.
Study Literature: Role of Secretome Mesenchymal Stem Cell as Innovation in Burn Treatment Desti Wulandari; Marlina Marlina; Rahmi Novita
JURNAL PROTEKSI KESEHATAN Vol 12 No 1 (2023): JPK: Jurnal Proteksi Kesehatan
Publisher : Poltekkes Kemenkes Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36929/jpk.v12i1.635

Abstract

The purpose of this review was to determine the role of MSCs in burn management. Scientific publication databases such as Researchgate, Google Scholar, ScienceDirect, and Pubmed were used for literature searches. [(Secretome, Mesenchymal stem cell Adipose Tissue, Umbilical Cord, Bone Marrow) AND (Burn wound OR wound healing OR burn injury OR thermal injury)] are the keywords used. In ten studies, rats, mice, and humans were used as research subjects in randomized clinical trials to conduct synthetic analyses. MSC therapy involves the use of bone marrow, cord blood, and adipose tissue. MSC therapy heals burns by suppressing the inflammatory response and oxidative stress, stimulating angiogenesis, and causing closure and repair (remodeling). MSC therapy has also been shown to reduce scarring and contracture formation.
Literatur Review: Prospects of Stem Cell Secretomes for the Treatment of Hair Loss/Alopecia Nella Novita; Marlina Marlina; Regina Andayani
JURNAL PROTEKSI KESEHATAN Vol 12 No 1 (2023): JPK: Jurnal Proteksi Kesehatan
Publisher : Poltekkes Kemenkes Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36929/jpk.v12i1.666

Abstract

The goal of this literature review is to find out the role of MSC in stimulating hair growth, particularly in people who suffer from hair loss/alopecia. Literature reviews were conducted using an electronic database such as Researchgate, PubMed, GoogleScholar, and ScienceDirect. MSC secretome for hair growth, secretome for hair growth, ADSC-Exosome for hair growth, ADSC-CM for alopecia, secretome conditioned medium for baldness, and formulation of hair tonic preparations to stimulate hair growth were the keywords used. From these keywords, 13 relevant research articles were found, 11 of which used patients with alopecia as research subjects in randomized clinical trials to examine hair growth. MSC therapy for alopecia has been shown to stimulate hair growth, density, and thickness.
Aplikasi Agarosa sebagai Matriks Kultur 3d pada Sel Kanker Paru Line A549 Suci Rahmawati; Adek Zamrud Adnan; Muhammad Taher; Marlina Marlina
Jurnal Vokasi Keperawatan (JVK) Vol. 2 No. 2 (2019): DESEMBER
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/jvk.v2i2.10690

Abstract

Agarosa merupakan salah satu biopolimer yang sering digunakan dalam bidang bioteknologi, diantaranya sebagai matriks pada kultur sel. Penelitian ini bertujuan untuk mengaplikasikan agarosa hasil isolasi dari tepung agar sebagai matriks kultur 3D pada sel kanker paru line A549. Hasil isolasi diperoleh Agarosa 1 (A1) dan Agarosa 2 (A2) hasil pemurnian dari A1. A1 dan A2 dengan konsentrasi 5% diaplikasikan sebagai matriks kultur 3D di dalam well plate kultur sel kanker paru line A549 dengan densitas 107, sebagai kontrol pertumbuhan digunakan kultur 2D sel (well plate tanpa matriks) dan kontrol positif kultur 3D sel pada matriks agarosa Top Vision®. Viabilitas sel diamati pada hari ke 2, 4 dan 6 menggunakan pewarna trypan blue. Proliferasi sel diamati pada hari ke 6 menggunakan reagen MTT. Hasil uji viabilitas terlihat agregat sel 3D pada matriks agarosa yang viable dan tidak menyerap warna trypan blue. Viabilitas sel dibuktikan dengan uji proliferasi dan diperoleh nilai rata-rata absorban pada kultur 2D, kultur 3D matriks kontrol, A1 dan A2 masing-masingnya 2.03; 1.21; 0.96 dan 1.22. Proliferasi kultur 2D lebih baik dari 3D, akan tetapi proliferasi kultur 3D pada matriks A2 lebih baik dari matriks kontrol. Pada penelitian ini dapat disimpulkan bahwa agarosa dapat diaplikasikan sebagai matriks kultur 3D sel
Swiftlet Bird's Nest (Collocalia fuciphaga) from West Sumatra as a Functional Food and Its Nutritional Content Aditya Willy Putra; Endang Purwati; Marlina
Andalasian Livestock Vol. 1 No. 1 (2024): ALive
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/alive.v1.n1.p9-17.2024

Abstract

Functional foods contain biologically active components and compounds that have physiological effects on the human body, such as promoting health and decreasing the risk of sickness. The swiftlet, which belongs to the Collocalini family, is an insect-eating bird that migrates across the Indian Ocean, Southeast Asia, and northern Australia to the Pacific Ocean. Swiftlet bird's nests (SBN) contain protein, fats, carbohydrates, iron, calcium, phosphorus, inorganic salts, fiber, and water. The objective of this study is to determine the nutritional content of swiftlet bird nests in different regions of West Sumatra. Samples of swiftlet bird's nests (SBN) were collected from Padang City (PD), Bukittinggi City (BK), and Pesisir Selatan Regency (PS). Nutritional content was analyzed using proximate methods, including moisture, protein, and fat, and the nitrite concentration was measured using UV-Vis spectrophotometry. The study found that SBN in West Sumatra contains 47.65–50.55% protein, 1.48–2.47% fat, 21.77–22.77% water, and 24.75–32.72 ppm nitrite. The results indicate that the nutritional content varies among the samples, and the protein content found in SBN is higher, making it suitable for use as a functional food. Additionally, SBN has many health benefits and biological activities, such as mitogenic response and cell growth, enhanced immunity, and improved bone strength.
Co-Authors A. Tubagus Surur Adek Zamrud Adnan Aditya Willy Putra Agus Wartiningsih Akmal Djamaan AM. M. Hafidz MS Andani Eka Putra Arfiandi Arfiandi Armenia, Armenia Asrori . Densi Selpia Sopianti Densi Selpia Sopianti Desti Wulandari Dewi Gulyla Hari Endang Purwati Fitri Amalinda Hajidin . Hanna Sari Widya Kusuma Harfia Mudahar Ika Adhani Sholihah Ikhwan Resmala Sudji Irene Puspa Dewi Iwan Kandori M Ikhsan Martono . Mitsuaki Nishibuchi Muhammad Taher Nella Novita P Pachaly Rahmi Novita Regina Handayani Handayani, Regina Handayani Rizal Rizal Rizki Rahmadian Rumbayan, Linda Rustini Rustini Satrio Haryo Benowo Wibowo Sesilia Seli Son Radu Suci Rahmawati Surya Dharma Vika Amelia Wahyu Setia Widodo Wahyu Widowati Yufri Aldi Yusrizal .