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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia Medical Journal of Indonesia Berkala Ilmu Kesehatan Kulit dan Kelamin Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Ilmu Kesehatan dan Gizi

INDROPO AGUSNI

Dept. Of Dermatology, School Of Medicine, Universitas Airlangga, Surabaya

Author-ID : 221708

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara Adriaty, Dinar; Wahyuni, Ratna; Iswahyudi, Iswahyudi; Aksono, Bimo; Koesbardiati, Toetik; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.2.3.%x

The existence of leprosy or kusta or Morbus Hansen or Hansens disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms).

Molecular Detection of Dapsone and Rifampicin Resistance on Mycobacterium leprae from Leprosy Patients in East Java DINAR ADRIATY; RATNA WAHYUNI; CITA ROSITA S. PRAKOESWA; NI PUTU SUSARI; INDROPO AGUSNI; SHINZO IZUMI
Microbiology Indonesia Vol. 3 No. 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

The drug resistant problem of Mycobacterium leprae has been developing since the last decade and this has become a leprosy elimination problem in several countries, including Indonesia. Using biological on molecular methods, it is now possible to test for drug resistant cases in relatively simple and less time consuming ways. The purpose of the study is to analyze theprevalence of drug resistance M. leprae to dapsone and rifampicin in East Java based on the etection of mutations in the folP and rpoB genes. All samples were obtained from multibacillary leprosy patients in East Java, who have admitted to the Dr Sutomo Hospital Surabaya in 2003-2005. Isolates were analyzed by PCR, and the presence of nucleotide sequence of the folP and rpoB genes from M. leprae were confirmed by direct sequencing. Of 94 specimens which were collected, all were analyzed for their folP and rpoB genome. From 94 isolates, 70 showed a positive result by the folP1-folPR test and 77 out of 94 isolates showed positive by the rpoBF-rpoBR test. From 70 isolates for folP gene examination, there were 3 isolates which had mutation in the amino acid at codon 53; 2 cases Threonin (ACC) became Alanin (GCC) and 1 case Threonin (ACC) became Arginin (AGA). These mutations are responsible to dapsone resistance. For the rpoB gene, no mutation was found. The result suggested that 3 isolates (4.3%), 1 from a new case and 2 from relapse cases in this experiment, were resistant to dapsone and all isolates (100%) were susceptible to rifampicin.

Profile of Mutation on Drug Resistance Mycobacterium leprae Isolates in Indonesia Collected During 2003-2011 RATNA WAHYUNI; DINAR ADRIATY; ISWAHYUDI ISWAHYUDI; CITA ROSITA SIGIT PRAKOESWA; INDROPO AGUSNI; SHINZO IZUMI
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Multidrug therapy (MDT) regiment has been used for leprosy all over the world for more than 20 years. Drug resistances of Mycobacterium have been reported from many areas. The resistance mostly occurred due to mutation on the gene coding protein targeted by anti-leprosy drugs. Two hundreds and seventy M. leprae isolates from some area in Indonesia were examined for studying the profile of mutation among isolates collected during 2003-2011. Drug resistance determining region of the folP1 gene and the rpoB gene was sequenced. The results showed 5 isolates of M. leprae harboured mutation only in the folP1 gene and another isolate harbored mutation in both the folP1 and rpoB gene. The point mutation in the folP1 gene that was found in 2 isolates occurred in codon 53 (ACC→GCC; Thr→Ala). Double point mutations on codon 53 that was found in two isolates were ACC→AGA (Thr→Arg) and ACC→AGG (Thr→Arg). The point mutation in the folP1 geneoccurred in codon 55 were found in two isolates were CCC→CTC (Pro→Leu) and CCC→CGC (Pro→Arg). Whereas mutation in the rpoB gene in one isolate occurred in codon 410 was GAT→TAT (Asp→Tyr). These mutations that altered the amino acids of the protein revealed that isolates of M. leprae were resistant to drug with variable profiles

Profil Pasien Kusta Baru pada Anak I G.A. Kencana Wulan; Indropo Agusni; Cita Rosita
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 26 No. 2 (2014): BIKKK AGUSTUS 2014
Publisher : Faculty of Medicine, Universitas Airlangga

Latar belakang: Kusta hingga saat ini masih menjadi masalah kesehatan di Indonesia. Kusta pada anak merupakan indeks epidemiologis untuk menentukan transmisi penyakit serta sebagai salah satu indikator dalam keberhasilan program pemberantasan penyakit kusta nasional. Tujuan: Mengevaluasi profil pasien kusta pada anak. Metode: Penelitian retrospektif terhadap semua kasus kusta baru pada anak (0-14 tahun) yang datang ke Unit Rawat Jalan (URJ) Kesehatan Kulit dan Kelamin RSUD Dr.Soetomo Surabaya mulai Januari 2009 sampai Desember 2011. Hasil: Didapatkan 37 pasien anak (5,5%) dari 677 kasus kusta baru. Kasus paling banyak (70,3%) terdapat pada kelompok usia 10-14 tahun. Laki-laki lebih banyak daripada perempuan. Berdasarkan klasifikasi WHO, sebanyak 29 kasus (78,4%) adalah kusta multibasiler (MB) dan 8 kasus (21,6%) adalah kusta pausibasiler (PB). Tipe terbanyak adalah kusta tipe BB (59,5%) pada kelompok MB dan diikuti tipe TT (16,2%) pada kelompok PB. Seluruh kasus mendapat pengobatan Multidrug Therapy (MDT) sesuai panduan WHO. Kontak serumah didapatkan 68,7% kasus. Simpulan: Ditemukan 37 kasus kusta baru pada anak di RSUD Dr. Soetomo Surabaya selama tiga tahun, terutama pada kelompok umur 10-14 tahun, dengan kasus terbanyak berupa kusta MB tipe BB. Kontak serumah masih memegang peranan yang penting pada transmisi kusta.Kata kunci: kusta, anak-anak, retrospektif.

Reverse Transcription Polymerase Chain Reaction (RT-PCR) untuk Mendeteksi Viabilitas Mycobacterium leprae pada Pasien Kusta Tipe Multibasiler Pascapengobatan MDT-WHO Lunni Gayatri; M. Yulianto Listiawan; Indropo Agusni
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 26 No. 2 (2014): BIKKK AGUSTUS 2014
Publisher : Faculty of Medicine, Universitas Airlangga

Latar belakang: Kusta masih menjadi masalah besar di Indonesia. Penatalaksanaan dengan MDT-WHO telah menjadi regimen standar untuk pasien kusta sejak lama. Pada pasien kusta tipe multibasiler (MB), indeks bakteriologis (IB) belum mencapai nilai nol, meskipun telah menyelesaikan terapi 12 regimen. Metode Reverse Transcription-Polymerase Chain Reaction (RT-PCR) memiliki sensitivitas tinggi untuk mendeteksi viabilitas M. leprae. Tujuan: Mengevaluasi viabilitas M. leprae dengan metode RT-PCR pada pasien kusta tipe MB yang telah menyelesaikan regimen MDT 12 di RSUD Dr. Soetomo Surabaya. Metode: 15 pasien kusta tipe MB yang telah menyelesaikan pengobatan MDT-WHO 12 regimen dengan IB positif dilakukan biopsi kulit untuk pemeriksaan RT-PCR. Hasil: Terdapat 13 pasien (86,7%) dengan viabilitas M. leprae positif sementara seluruh pasien tersebut (100%) memiliki Indeks Morfologis (IM) 0%. Dua belas pasien (80%) memiliki Indeks Bakteriologis 2+ dan 10 pasien diantaranya adalah pasien kusta tipe Lepromatous Leprosy (LL) polar. Delapan pasien yang memiliki viabilitas positif tidak mendapatkan kortikosteroid oral selama siklus MDT. Simpulan: Pasien kusta yang telah menyelesaikan terapi MDT dengan MI 0%, didapatkan viabilitas M. Leprae positif dengan metode RT-PCR. Spektrum klinis pasien yang ditentukan dari imunitas seluler nampaknya menjadi faktor terpenting dalam viabilitas M. leprae.Katakunci: viabilitas, M. leprae, kusta tipe multibasiler, RT-PCR.

Secreted Aspartyl Proteinase[SAP] Enzyme Proﬁle on Vulvovaginalis Candidiasis Patient's isolates Diah Nugrahaeni; Indropo Agusni; Evy Ervianti
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 27 No. 1 (2015): BIKKK APRIL 2015
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Vulvovaginalis candidiasis (VVC) is an infection caused by various types of Candida, themost is Candida albicans. Candida produces two main enzymes that play a role in virulence, the secreted aspartyl proteinase (SAP) enzyme and the phospholipase enzyme. SAP is most widely produced by Candida albicans,which is one of the main pathogenicfactor of Candida infection. SAP is often found in cases of recurrent candidiasis vulvovaginalis (RVVC) Purpose: To evaluatethe SAP activity produced by VVC patients isolates. Methods: Descriptive observational cross-sectional study was conducted on 21 VVC patients to determine the SAP enzyme levels in VVC patients in Sexually Transmitted Infection (STI) Division, Outpatient Clinic Of Dermatology and Venerology, Dr. Soetomo General Hospital, Surabaya.Vaginal smear was examined to determine levels of SAP and culture examination to determine the kind of candida spesies that causes VVC. Results: Total subject of study are 21 patients. Twelve of 21 patients had SAP > 20 mm in diameter, 7 patients with SAP < 20 mm in diameter, and 2 patients with negative results of SAP. Fifty patients due to Candida albicans, 6 patients caused by non-albicans. Nine patients were accompanied by diabetes mellitus (DM), 1 patients by genital affection, 2 patients by condylomata acuminata. Additionally obtained in 10 patients who had previously received treatment with the results diameter SAP > 20 mm and 7 patients with of SAP diameter < 20 mm, 2 patients showed negative results of SAP. Conclusions: SAP was mainly produced by Candida albicans. SAP became more active in VVC patients that accompanied by diabetes melitus, affectiogenitalis, condylomata acuminate, and also in some patients who have received prior antifungal therapy.Key words: secreted aspartyl proteinase, vulvovaginalis candidiasis, Candida albicans,

Oral Corticosteroid Therapy in Leprosy's new patients with Type 2 Reaction Irma Tarida Listiyawati; Sawitri Sawitri; Indropo Agusni; Cita Rosita Sigit Prakoeswa
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 27 No. 1 (2015): BIKKK APRIL 2015
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Leprosy type 2 reactions is an acute inflammatory episode in lepromatous leprosy patient that ascociated with deposition of immune complexes. Standard regiment for severe reaction is corticosteroid. Almost Leprosy type 2 reactions patients become chronic and recurrent so it implicates to more complications if it was not managed well. Purpose: To determine the distribution of type 2 reactions patients which treated with oral corticosteroid in leprosy division of dermatovenereology outpatient clinic of Dr. Soetomo General Hospital. Methods: Retrospective study using new patient's medical record during the period of January 1 2009 until December 31 2013 and the observation continued until December 31 2013. Results: The total type 2 reactions patients with oral corticosteroid was 112 patients, mostly were male aged between 25-64 years and has the Lepromatous Leprosy (LL) type (33%), the reactions happened after RFT (43.8%), 65.2% had metil prednisolon medication, the initial equivalent dose was 30 mg in 32.1% patient. 21.4% patient had more than one cycle of corticosteroid therapy and 8.9% patient had the steroid side effect. Conclusion: Leprosy's type 2 reactions tend to be chronic and recurrent and the impact were patients got longterm continuously corticosteroid medication, so the patients need to be monitored including side effects.Key words: leprosy's type 2 reaction, oral corticosteroid, side effects.

Immunomodulators for a Variety of Viral infections of the Skin I G A Kencana Wulan; Indropo Agusni
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 27 No. 1 (2015): BIKKK APRIL 2015
Publisher : Faculty of Medicine, Universitas Airlangga

Background: In the last decade there has been inceasing prevalence of viral infections in the world, this led to increased efforts to find a variety of new drugs and vaccines against the virus that causes. Purpose: To disccuss the provision relating to the use of immunomodulators in viral infections. Review: Some diseases caused by viruses such as varicella, herpes zoster, herpes simplex, condyloma acuminata, molluscum contagiosum and Human immunodeficiency syndrome (HIV). Immunomodulator also known as biological response modifier, a wide variety of materials imunoaugmentor is either recombinant, synthetic, or natural medicines that returns an imbalance of the immune system that is used in immunotherapy. Immunotherapy is a treatment approach by restoring, enhancing, or suppresing the immune response. Immunomodulator administration on viral infections gave varying results. Conclusion: Immunomodulatory regimens on viral infections is an attractive therapeutic approach, because the side effects are often lighter than the side effects of drugs that have been there, in addition to the more rarely cause resistance to treatment of diseases caused by viral infection.Key word: viral infection, immunomodulator, immune response.

Expression of Transforming Growth Factor–Beta (TGF-β) on Regulatory T (Treg) Cell from Skin Lesion of Leprosy’s Patient Kristina Sihaloho; Afif Nurul Hidayati; Indropo Agusni
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 28 No. 1 (2016): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Clinical manifestations of leprosy depends on host’s immunity. Regulatory T (Treg) cells are known as CD4+CD25+FOXP3+ have roles in immune respons to maintain homeostasis. Transforming growth factor (TGF)-β are one of cytokine that is secreted by Treg cells. Treg cells are thought as unresponsiveness T cells (anergy) to Mycobacterium leprae and caused disease’s progression to lepromatosa. Purpose: To evaluate expression of TGF-β on Treg cells from patient leprosy’s skin lession. Methods: Design of study was cross-sectional, descriptive observation involving 18 leprosy patients who visited the Leprosy Division, Dermatology and Venereology Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya. Biopsy from skin lession were performed then continued with histopathological examination. The type of leprosy was determined by clinical and histopathological examinations. Expression of TGF-β on Treg cell were performed by double staining immunohistochemistry in Biomoleculer and Pathology and Anatomy Laboratory at Brawijaya University, Malang. Result: Numbers of mid-borderline type (BB) leprosy’s patients were 2 (11,1%), 9 (50%) patients with borderline lepromatosa (BL) type, and 7 (38,5%) patients with Lepromatosa (LL) type. Mean expression of TGF-β on Treg cells from skin lession’s leprosy on BB type was 12 ± 2,12 cells/field of view, on BL type was 16 ± 3,64 cells/fields of view and on LL type was 22± 3,69 cells/field of view. Conclusion: Expression of TGF-β on Treg cells are increasing on skin lession’s leprosy patients might as prognostic indicator of severity of leprosy (lepromatosa type). Key words: immunohistochemistry, leprosy, Morbus Hansen, regulatory T cell, TGF-β.

Higher Level Zinc Serum in Multibacillary Leprosy With Erythema Nodosum Leprosum compared to Patients Without Reaction Kinanti Prabawaningrum; Linda Astari; Indropo Agusni
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 28 No. 1 (2016): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Leprosy is an infectious disease that causes a redistribution of nutrients in the body, including zinc, and low food intake of zinc causes decreased serum zinc levels. The number of bacteria M. leprae in multibacillary (MB) type leprosy and acute inflammatory process in Erythema Nodosum Leprosum (ENL) reaction affect the levels of zinc. Purpose: To analyze differences in the results of serum zinc levels in MB patients without reactions and ENL. Methods: Cross sectional study was conducted on forty-eight patients with a diagnosis of MB leprosy in Dermato and venereology Outpatient Clinic Dr. Soetomo General Hospital Surabaya. Patient's blood had been taken to examine serum zinc levels. Result: The mean serum zinc levels of MB patients without reaction 78.49 g/dL, while ENL 175.83 g/dL. Conclusion: The mean serum zinc levels of MB patients with ENL is higher than without reaction.Key words: MB leprosy, ENL, cross sectional, serum zinc.

Co-Authors A. M. Adam, A. M. Afif Nurul Hidayati Afif Nurul Hidayati, Afif Nurul Agustina Tri Pujiastuti Anggraeni, Sylvia Anis Irawan Anwar Astindari Astindari, Astindari Aulia, Syifa Bambang Wirjatmadi Bimo Aksono Bimo Aksono Cita Prakoeswa, Cita Cita Rosita CITA ROSITA S. PRAKOESWA CITA ROSITA S. PRAKOESWA Cita Rosita Sigit Prakoeswa Cita Rosita, Cita Diah Mira I Diah Nugrahaeni Diah Nugrahaeni, Diah DINAR ADRIATY Dinar Ardiaty, Dinar Ditta Harnindya Dwi Murtiastutik Endang Retnowati Evy Ervianti Fanny Iskandar, Fanny Farida Tabri Febrina Dewi Pratiwi Friska Jifanti Halik Wijaya I G A Kencana Wulan I G A Kencana Wulan, I G A Kencana I G.A. Kencana Wulan I G.A. Kencana Wulan, I G.A. Kencana Icha Aisyah Indah Sari LD Irawan Yusuf Irma Tarida Listiyawati Irma Tarida Listiyawati, Irma Tarida ISWAHYUDI ISWAHYUDI Iswahyudi Iswahyudi Kinanti Prabawaningrum Kristina Sihaloho Linda Astari, Linda Lunni Gayatri Lunni Gayatri, Lunni Lydiawati, Eva M. dali amiruddin, M. dali M. Yulianto Listiawan Mansur Arif, Mansur Maylita Sari, Maylita Meita Ardini Pratamasari, Meita Ardini Mohammad Z. Rahfiludin Muhammad Dali Amiruddin Netty Sukmawati, Netty NI PUTU SUSARI Nuswantoro, Djohar Prakoeswa, Cita Rosita rachmah diana putri, rachmah diana Rachmawati Rachmawati Rahmadewi Rahmadewi Rahmatari, Bandaru Rasyidin Abdullah Ratana, Heng Ratna Wahyuni RATNA WAHYUNI Rinasari, Umi Safruddin Adam, Safruddin Safruddin Amin Santoso, Rachmat Sawitri Sawitri Sawitri Sawitri Sepling Paling SEPTIANA SEPTIANA Sheilaadji, Maria Ulfa Shinzo Izumi SHINZO IZUMI SHINZO IZUMI SHINZO IZUMI Sirithida, Chukmol Siti Kurniawati Tasalina Yohana Parameswari Gustam Timurleng Tonang Mataallo, Timurleng Tonang Toetik Koesbardiati Vannda, Sou Vortey, Hak Willy Sandhika Yoes P. Dahlan Yuniarti Arsyad, Yuniarti Yuri Widia, Yuri Yusuf Wibisono

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