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Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
Co-Authors A. M. Adam, A. M. Afif Nurul Hidayati Afif Nurul Hidayati, Afif Nurul Agustina Tri Pujiastuti Anggraeni, Sylvia Anis Irawan Anwar Astindari Astindari, Astindari Aulia, Syifa Bambang Wirjatmadi Bimo Aksono Bimo Aksono Cita Prakoeswa, Cita Cita Rosita CITA ROSITA S. PRAKOESWA CITA ROSITA S. PRAKOESWA Cita Rosita Sigit Prakoeswa Cita Rosita, Cita Diah Mira I Diah Nugrahaeni Diah Nugrahaeni, Diah DINAR ADRIATY Dinar Ardiaty, Dinar Ditta Harnindya Dwi Murtiastutik Endang Retnowati Evy Ervianti Fanny Iskandar, Fanny Farida Tabri Febrina Dewi Pratiwi Friska Jifanti Halik Wijaya I G A Kencana Wulan I G A Kencana Wulan, I G A Kencana I G.A. Kencana Wulan I G.A. Kencana Wulan, I G.A. Kencana Icha Aisyah Indah Sari LD Irawan Yusuf Irma Tarida Listiyawati Irma Tarida Listiyawati, Irma Tarida ISWAHYUDI ISWAHYUDI Iswahyudi Iswahyudi Kinanti Prabawaningrum Kristina Sihaloho Linda Astari, Linda Lunni Gayatri Lunni Gayatri, Lunni Lydiawati, Eva M. dali amiruddin, M. dali M. Yulianto Listiawan Mansur Arif, Mansur Maylita Sari, Maylita Meita Ardini Pratamasari, Meita Ardini Mohammad Z. Rahfiludin Muhammad Dali Amiruddin Netty Sukmawati, Netty NI PUTU SUSARI Nuswantoro, Djohar Prakoeswa, Cita Rosita rachmah diana putri, rachmah diana Rachmawati Rachmawati Rahmadewi Rahmadewi Rahmatari, Bandaru Rasyidin Abdullah Ratana, Heng RATNA WAHYUNI Ratna Wahyuni Rinasari, Umi Safruddin Adam, Safruddin Safruddin Amin Santoso, Rachmat Sawitri Sawitri Sawitri Sawitri Sepling Paling SEPTIANA SEPTIANA Sheilaadji, Maria Ulfa SHINZO IZUMI Shinzo Izumi SHINZO IZUMI SHINZO IZUMI Sirithida, Chukmol Siti Kurniawati Tasalina Yohana Parameswari Gustam Timurleng Tonang Mataallo, Timurleng Tonang Toetik Koesbardiati Vannda, Sou Vortey, Hak Willy Sandhika Yoes P. Dahlan Yuniarti Arsyad, Yuniarti Yuri Widia, Yuri Yusuf Wibisono