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All Journal HAYATI Journal of Biosciences Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Zona Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati Organisms: Journal of Biosciences
Anthoni Agustien
Universitas Andalas
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Zam, Syukria Ikhsan; Agustien, Anthoni; Syamsuardi, Syamsuardi; Djamaan, Akmal; Mustafa, Irfan
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Syukria Ikhsan Zam; Anthoni Agustien; Syamsuardi Syamsuardi; Akmal Djamaan; Irfan Mustafa
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
Co-Authors - Jumawita . Mansyurdin Aghni Aznia Akmal Djamaan Alviche, Lola Anggun Sophia Annisa Farma Annisa Khaira Armaleni Armaleni Asih, Enda Tarni Ayu Putri Ningsih Ayumi Rizci Puspita Chairul Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dianty Putri Dina Wahyuna Djong Hon Tjong Dwitaviani, Rima Efrizal Efrizal Efrizal Efrizal Endang Purwati RN Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Herwita Idris Hesti Dwi Marcellinna Ikel Fitri Yanis Ilham, Kurniadi Ilsa Septia Putri Irdawati Irdawati Irfan Mustafa Jetty Nurhajati Kurniadi Ilham Kustiasih Lestari Linar Z. Udin Lola Alviche Maliza, Rita Marlina Marlina Melisa Ainil Fajri Mellanie Alia Putri Mifthahul Jannah Mildawati Mildawati Mira Rosnawita Mohsin Rafique Muhammad Diki Juliandi Muqarramah, Mufidhatul Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Periadnadi Periadnadi Pingkan Aditiawati PINGKAN ADITIAWATI Putri, Silvy Rizka Rahmadani Eka Pitri Rahmayati, Riesca Salsabilah Rahmi Adila Rahmi Inayati Riesca Salsabilah Rahmayati Riski Budi Yani RR. Ella Evrita Hestiandari Rusnam Rusnam Shinta, Dewi Yudiana Suwirmen, Suwirmen Syafrita, Assyifa Syamsuardi Syamsuardi Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Triyatdipa, Haqil Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Wiwik Handayani Yetria Rilda Yetti Marlida Yetty Marlida Yurike Nova Edlin Zakaria, Indra Junadi Zovia, Miftahul Zozy Aneloi Noli Zuhri Syam Zuhri Syam