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DISEMINASI TEKNOLOGI BUDIDAYA IKAN NILA NIRWANA INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Chairul Chairul; Anthoni Agustien; Nurmiati Nurmiati; Zuhri Syam; Suwirmen Suwirmen; Ferry Lismanto Syaiful
Jurnal Hilirisasi IPTEKS Vol 1 No 4.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.159 KB)

Kegiatan IPTEKS berbasis dosen & masyarakat (IbDM) ini dilakukan dengan tujuan untuk diseminasi teknologi budidaya ikan Nila Nirwana secara intensif dalam upaya meningkatkan produktivitas perikanan di Kelurahan Limau Manis, Kecamatan Pauh, Kota Padang dan meningkatkan pengetahuan serta keterampilan petani ikan (masyarakat) Kelurahan Koto Tangah dalam bidang usaha pemeliharaan ikan Nila Nirwana di Sumatera Barat, sehingga diharapkan mereka dapat melakukan pemeliharaan dengan cara/teknik yang baik dan ramah lingkungan. Selain itu kegiatan IbDM ini juga diharapakan mampu mengurangi biaya operasional dalam pemeliharaan ikan Nila Nirwana dan pada akhirnya dapat meningkatkan pendapatan para petani (masyarakat) Kelurahan Limau Manis melalui pengembangan usaha budidaya ikan yang berkelanjutan. Untuk mencapai hasil diseminasi teknologi yang optimal maka sasaran utama IbDM di Kecamatan Pauh ini adalah kelompok tani ikan dan para pembina/pemuka masyarakat Kelurahan Limau Manis yang diharapkan mampu memberikan motivasi kepada petani untuk mensosialisasikan metoda pembuatan pakan formula alternatif untuk pengembangan budidaya ikan Nila Nirwana secara intensif dan ramah lingkungan. Metoda yang digunakan dalam kegiatan ini adalah (1) Penyuluhan (ceramah), dengan materi utama : (a) memberikan pengetahuan tentang berbagai macam bahan baku yang dapat dimanfaatkan untuk makanan ikan, (b) memberikan pengetahuan tentang penyusunan formula/komposisi makanan buatan, (c) memberikan pengetahuan tentang proses pembuatan makanan buatan (d) menambah pengetahuan masyarakat tentang cara menyimpan pelet yang baik agar lebih tahan lama dan (e) pemberian materi tentang pengujian mutu pakan, baik secara fisika, kimia dan biologis; dan (2) Peragaan pembuatan pakan pelet alternatif untuk budidaya ikan Nila Nirwana secara intensif. Dari hasil kegiatan IbDM ini didapatkan bahwa pengetahuan dan keterampilan kelompok tani Kelurahan Limau Manis Kec. Pauh, Padang sebelum dilaksanakan kegiatan IbDM ini masih sangat rendah. Namun demikian motivasi atau keinginan masyarakat kelompok tani mitra IbDM untuk melakukan usaha budidaya atau pemeliharaan ikan sangat besar. Hal ini terlihat dari keinginan masyarakat kelompok tani mitra IbDM untuk menguasai teknologi pembuatan pakan ikan dari hasil limbah daun dan batang talas yang diproses menjadi tepung relatif cukup tinggi. Lebih jauh dari hasil kegiatan IbDM pada masyarakat ini dapat menambah pengetahuan dan keterampilan masyarakat dalam mencari pakan alternatif untuk pakan ikan sehingga kegiatan budidaya dapat berjalan dengan baik di daerah mitra IbDM. Agar kegiatan budidaya berjalan dengan baik di daerah mitra IbDM perlu dilakukan kegiatan IbDM secara berkelanjutan tentang teknik pembuatan pelet ikan alternatif dan teknik pemeliharaan ikan yang efisien dan efektif. Disamping itu perlu dukungan dana atau pemberian kredit oleh pihak yang berwenang kepada masyarakat atau petani ikan untuk modal pengembangan usahanya.

DISEMINASI TEKNOLOGI PAKAN BUATAN ALTERNATIF UNTUK PENGEMBANGAN BUDIDAYA IKAN GURAME INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Nurmiati Nurmiati; Chairul Chairul; Anthoni Agustien; Suwirmen Suwirmen; Rusnam Rusnam; Deswati Deswati
Jurnal Hilirisasi IPTEKS Vol 3 No 1 (2020)
Publisher : LPPM Universitas Andalas

Lecturer and community based science and technology activities (IbDM) were carried out with the aim of disseminating technology for Gurame culture intensively in an effort to increase fisheries productivity in Limau Manis, Pauh Subdistrict, Padang City and increase the knowledge and skills of fish farmers (community) in Koto Tangah Village in the field of Gurame fish maintenance business in West Sumatra, so that they are expected to be able to carry out maintenance in a good / environmentally friendly manner. Besides this IbDM activities are also expected to be able to reduce operational costs in maintaining Nirwana Tilapia and in the end can increase the income of the farmers (community) of Limau Manis Village through the development of sustainable fish farming. To achieve optimal technology dissemination results, the main target of IbDM in Pauh Subdistrict is fish farmer groups and community leaders / leaders of Limau Manis Village who are expected to be able to motivate farmers to socialize the method of making alternative formula feeds for intensive development of Nirwana Tilapia aquaculture and environmentally friendly. The methods used in this activity are (1) counseling, with the main material: (a) providing knowledge of various kinds of raw materials that can be used for fish food, (b) providing knowledge about the preparation of artificial food formulas / compositions, ( c) provide knowledge about the process of making artificial food (d) increase public knowledge about how to store pellets that are good to be more durable and (e) provision of material on testing the quality of feed, both physically, chemically and biologically; and (2) Intensive demonstration of making alternative pellet feeds for Nirwana Tilapia aquaculture. From the results of the IbDM activity, it was found that the knowledge and skills of farmer groups in Limau Manis Sub-district, Kec. Pauh, Padang before the IbDM activities were carried out was still very low. However, the motivation or desire of the community of IbDM partner farmer groups to carry out the culture or maintenance of fish is very large. This can be seen from the desire of the IbDM partner farmer group to master the technology of making fish feed from the results of leaf waste and taro stems which are processed into flour relatively high. Furthermore, the results of IbDM activities in the community can increase the knowledge and skills of the community in finding alternative feed for fish feed so that culture activities can run well in IbDM partner areas. In order for aquaculture to run well in partner areas of IbDM, it is necessary to carry out IbDM activities continuously on the techniques of making alternative fish pellets and efficient and effective fish-raising techniques. Besides that, it needs financial support or credit by the authorities to the community or fish farmers for capital to develop their businesses.

DISEMINASI TEKNOLOGI FORMULATED DIET ALTERNATIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS BUDIDAYA IKAN NILA INTENSIF DI KOTA PADANG Efrizal Efrizal; Nurmiati Nurmiati; Chairul Chairul; Anthoni Agustien; Zuhri Syam; Suwirmen Suwirmen; Rusnam Rusnam; Deswati Deswati
Jurnal Hilirisasi IPTEKS Vol 4 No 4 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i4.534

Fish cultivators generally experience difficulties providing quality artificial feed (pellets) due to a lack of knowledge and skills in processing technology and making alternative feeds. Besides that, the costs incurred for feed procurement are relatively large, reaching 70 - 80% of the total production costs. However, the provision of feed is often an obstacle because, in addition to the increasingly high price, the quality of available feed does not always match the nutritional needs needed by Fish. The provision of quality feed for intensively cultivated tilapia dramatically affects the resulting growth and productivity. The higher the quality of the feed, the production will also increase. One of the technologies that can be applied to overcome this problem is the manufacture of alternative formulated diets using local raw materials. The method used in this service activity is the transfer of knowledge presented in the socialization activities in the form of 1) introduction to the types of alternative fish feed ingredients sourced from the area, (2) preparation of artificial tilapia feed formulations, (3) manufacturing technology artificial feed (pellets) and 4) monitoring and assistance. The socialization was carried out in the form of a lecture and was followed by a question and answer session. The results of farmer activities recognize the types of local feed that can be used as fish feed, know the preparation of feed formulations, and alternative artificial feed manufacturing technologies. The knowledge and skills possessed by fish cultivators are expected to overcome the difficulties of fish farmers in providing feed so that fish production and cultivation business increase.

AMOBILISASI SEL Bacillus licheniformis KA-08 DALAM MENGHASILKAN KERATINASE TERMOSTABIL Anthoni Agustien
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Isolate local Bacillus licheniformis KA-08 known extracellular thermostable keratinase producers. Scale up of thermostable keratinase production can be with cells immobilized. The objective of the research is to thermostable keratinase production of B. licheniformis KA-08 cells immobilization. Thermostable keratinase activities were determined with modification of Brandelli and Riffel method. Protein concentration of enzyme determined with Lowry method. Immobilization of cells by Ca-alginate matrix with Adinarayana method, alginate concentration and amount of alginate bead effects with Beshay method. The result extracellular thermostable keratinase of B. licheniformis KA-08 cells immobilized was maximum produced at 12 times incubation with activity as 9.25 U/mg. Three percent alginate has optimum activity. Three hundred alginate beads has optimum activity. Cells immobilized of B. licheniformis KA-08 has scale up of thermostable keratinase activity at 2 times than free cells. Thermostable keratinase produced by cell immobilized was nine cycles.

The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Syukria Ikhsan Zam; Anthoni Agustien; Syamsuardi Syamsuardi; Akmal Djamaan; Irfan Mustafa
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).

Antibacterial Test of Ziziphus spina-christi (L.) Desf. Leaves Extract Againstgram-Positive Bacteria Feskaharny Alamsjah; Anthoni Agustien; Fadhila Cantika
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 24 No. 02 (2023): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol24-iss02/278

Ziziphus spina-christi (L.) Desf. is a member of the Rhamnaceae family, which has efficacy values as herbal plants used in traditional medicines. The spread of infectious diseases in humans has increased in recent years, raising public awareness of the importance of using traditional medicine as the first line of prevention against infectious diseases. This research aimed to determine the antibacterial activity of fresh extract, infusion, and ethanol extract and to determine the greatest inhibition zone of Z. spina-christi leaves against Gram-positive bacteria (B. cereus, B. subtilis, S. aureus, and S. epidermidis). This research used the survey method with a purposive sampling technique. The results showed that fresh extract, infusion, and ethanol extract of the Z. spina-christi leaves had antibacterial activity against Gram-positive bacteria (B. cereus, B. subtilis, S. aureus, and S. epidermidis) and the greatest inhibition zone was obtained from the ethanol extract with a strong inhibition category.

Uji Antibakteri Ekstrak Rimpang Koenih Rimbo (Curcuma sumatrana Miq.) Tumbuhan Endemik Sumatra Barat terhadap Bakteri Gram Positif Feskaharny Alamsjah; Anthoni Agustien; Tri Widia Ningsih Alam
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7697

Koenih rimbo (Curcuma sumatrana Miq.) is a medicinal plant endemic to Sumatra from the Zingiberaceae family. Research on the antibacterial test of koenih rimbo rhizome extracts against Gram-positive bacteria has been conducted at the Basic Biology Laboratory, UPT. Basic and Central Laboratory, Chemical Laboratory of Natural Materials, UPT. Biological Resources, Andalas University,Padang. This study aims to determine the antibacterial activity of fresh extracts, infusions, and ethanol extracts and determine the largest inhibition zone of koenih rimbo rhizomes against several Gram-positive bacteria. The research used a survey method with a purposive sampling technique. The results showed that the fresh extract, infusa, and ethanol extract had antibacterial activity against Gram-positive bacteria (Staphylococcus aureus, S. epidermidis, Bacillus subtilis, and B. cereus. The most significant inhibition zone was obtained from the extract with ethanol solvent with a strong inhibition category.

The Potential of Bacillus spp. In Green Biosynthesis of Zinc Oxide Nanoparticles Lola Alviche; Anthoni Agustien; Akmal Djamaan
Majalah Kedokteran Andalas Vol 46, No 5 (2023): Supplementary July 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i5.p818-835.2023

Nanopartikel yang dihasilkan melalui metode Green biosintesis berpotensi untuk diterapkan pada dunis medis dan farmasi. Penelitian ini menggunakan senyawa metabolit yang dihasilkan isolat Bacillus spp untuk mereduksi zink sulfat menjadi nanopartikel zink oksida. Berdasarkan hasil pengukuran spetrofotometri UV-Vis panjang gelombang optimum koloid nanopartikel yang dihasilkan dari proses Green biosintesis isolate BES 6A dengan penambahan zink sulfat sebanyak 0,2 g/ml yaitu pada 366 nm. Adapun gugus fungsi Zn-O berdasarkan pengukuran FTIR terbaca pada panjang puncak gelombang 438 dan 530 cm-1. Bentuk kristal yang dihasilkan yaitu heksagonal dengan puncak 2Ɵ yang terdeteksi yaitu 31,82°; 34,33°; 36,49°; 47,56°; 57,16°; 63,20°; 66,76°; dan 68,99° yang menandakan kehadiran nanopartikel zink oksida berdasarkan referensi ICDD 00-001-1136. Ukuran nanopartikel yang dihasilkan yaitu 30 – 47 nm.

Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms

Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms

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