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All Journal HAYATI Journal of Biosciences Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Best Journal (Biology Education, Sains and Technology) Jurnal Zona Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati
Anthoni Agustien
Universitas Andalas
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Potensi Jamur Endofit pada Tumbuhan Mangrove Avicennia marina di Kuala Enok Indragiri Hilir sebagai Penghasil Antibiotika Kustiasih Lestari; Anthoni Agustien; Akmal Djamaan
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p13

Abstract

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.
Pengaruh pH dan Suhu terhadap Produksi Antibiotika dari Isolat Bakteri Endofitik pada Tumbuhan Andalas (Morus macroura Miq.) Riski Budi Yani; Anthoni Agustien; Feskaharny Alamsjah
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p14

Abstract

The study used survey method and the data were analysed descriptively. The selection of the bacteria which produce antibiotic had been with paper disk method and used Escherichia coli and Staphylococcus aureus as the sample bacteria. This result showed pH 7,0 was succesful optimum pH antibiotic produced for Bacillus sp.1 and Bacillus sp.2. and 370C was the optimum temperature to antibiotic produced from Bacillus sp.1 and Bacillus sp.2.
Kemampuan Jamur Akar Putih (Rigidoporus microporus (Swartz: Fr.) Van Ov.) dalam Mendegradasi Limbah Selulosa Dianty Putri; Nasril Nasir; Anthoni Agustien
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p16

Abstract

Cellulose is one of abundance natural biopolymer with limited usage. The common found waste cellulose are waste straw, sawdust, and pineapple pit. Rigidoporus microporus is a white root fungi groups that often attack the roots of plants that rot and die. This study was conducted to assess the ability of white root fungi (R. microporus) to degrade waste cellulose, according to cellulolitic activity index of potential R. microporus isolate. The medium used was Carboxy Methyl Cellulose (CMC) with dye Congo Red (0,1%). The results obtained that R. microporus has cellulotic index 3,23 which indicated as potential cellulose degrade.
Antagonis Pseudomonas fluorescens indegenous terhadap Ralstonia solanacearum pada Tanaman Tomat (Lycopersicum esculentum) Armaleni Armaleni; Nasril Nasir; Anthoni Agustien
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p19

Abstract

Tomato (L. esculentum) is one of the most important vegetable crops produce in West Sumatra. In this province, tomato are cultivated at highland regions of Solok, Padang Panjang, Tanah Datar and Bukittinggi. Annually, the increasing demand of this crop comes from other provinces in Sumatra and Java. This opportunity stimulate tomato’s agribusiness activity in West Sumatra. However, the crop succumb to the most destroyed bacterial wilt disease caused by Ralstonia solanacearum. The aims of this study to select antagonist Pseudomonas fluorescent as a biological control agent against R.solanacearum. The candidates of antagonist P. fluorescetn were collected from diseased tomato with R. solanacearum’s symptom in several tomato’s plantations in West Sumatra. Study of the antagonist was conducted from March to July 2016 at the Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences Andalas University. To find the antagonist work, each of the collected candidate was grown opposite R. solanacearum on Nutrient Agar medium. The result found that there were16 isolates of P. fluorescent occurred. The best antagonist ability amongst the candidates was from Pfi with diameter zone was 4,95 cm.Keywords: tomato, Ralstonia solanacearum, Pseudomonas fluorescens, biological control agent, Pfi
DISEMINASI TEKNOLOGI BUDIDAYA IKAN NILA NIRWANA INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Chairul Chairul; Anthoni Agustien; Nurmiati Nurmiati; Zuhri Syam; Suwirmen Suwirmen; Ferry Lismanto Syaiful
Jurnal Hilirisasi IPTEKS Vol 1 No 4.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.159 KB)

Abstract

Kegiatan IPTEKS berbasis dosen & masyarakat (IbDM) ini dilakukan dengan tujuan untuk diseminasi teknologi budidaya ikan Nila Nirwana secara intensif dalam upaya meningkatkan produktivitas perikanan di Kelurahan Limau Manis, Kecamatan Pauh, Kota Padang dan meningkatkan pengetahuan serta keterampilan petani ikan (masyarakat) Kelurahan Koto Tangah dalam bidang usaha pemeliharaan ikan Nila Nirwana di Sumatera Barat, sehingga diharapkan mereka dapat melakukan pemeliharaan dengan cara/teknik yang baik dan ramah lingkungan. Selain itu kegiatan IbDM ini juga diharapakan mampu mengurangi biaya operasional dalam pemeliharaan ikan Nila Nirwana dan pada akhirnya dapat meningkatkan pendapatan para petani (masyarakat) Kelurahan Limau Manis melalui pengembangan usaha budidaya ikan yang berkelanjutan. Untuk mencapai hasil diseminasi teknologi yang optimal maka sasaran utama IbDM di Kecamatan Pauh ini adalah kelompok tani ikan dan para pembina/pemuka masyarakat Kelurahan Limau Manis yang diharapkan mampu memberikan motivasi kepada petani untuk mensosialisasikan metoda pembuatan pakan formula alternatif untuk pengembangan budidaya ikan Nila Nirwana secara intensif dan ramah lingkungan. Metoda yang digunakan dalam kegiatan ini adalah (1) Penyuluhan (ceramah), dengan materi utama : (a) memberikan pengetahuan tentang berbagai macam bahan baku yang dapat dimanfaatkan untuk makanan ikan, (b) memberikan pengetahuan tentang penyusunan formula/komposisi makanan buatan, (c) memberikan pengetahuan tentang proses pembuatan makanan buatan (d) menambah pengetahuan masyarakat tentang cara menyimpan pelet yang baik agar lebih tahan lama dan (e) pemberian materi tentang pengujian mutu pakan, baik secara fisika, kimia dan biologis; dan (2) Peragaan pembuatan pakan pelet alternatif untuk budidaya ikan Nila Nirwana secara intensif. Dari hasil kegiatan IbDM ini didapatkan bahwa pengetahuan dan keterampilan kelompok tani Kelurahan Limau Manis Kec. Pauh, Padang sebelum dilaksanakan kegiatan IbDM ini masih sangat rendah. Namun demikian motivasi atau keinginan masyarakat kelompok tani mitra IbDM untuk melakukan usaha budidaya atau pemeliharaan ikan sangat besar. Hal ini terlihat dari keinginan masyarakat kelompok tani mitra IbDM untuk menguasai teknologi pembuatan pakan ikan dari hasil limbah daun dan batang talas yang diproses menjadi tepung relatif cukup tinggi. Lebih jauh dari hasil kegiatan IbDM pada masyarakat ini dapat menambah pengetahuan dan keterampilan masyarakat dalam mencari pakan alternatif untuk pakan ikan sehingga kegiatan budidaya dapat berjalan dengan baik di daerah mitra IbDM. Agar kegiatan budidaya berjalan dengan baik di daerah mitra IbDM perlu dilakukan kegiatan IbDM secara berkelanjutan tentang teknik pembuatan pelet ikan alternatif dan teknik pemeliharaan ikan yang efisien dan efektif. Disamping itu perlu dukungan dana atau pemberian kredit oleh pihak yang berwenang kepada masyarakat atau petani ikan untuk modal pengembangan usahanya.
DISEMINASI TEKNOLOGI PAKAN BUATAN ALTERNATIF UNTUK PENGEMBANGAN BUDIDAYA IKAN GURAME INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Nurmiati Nurmiati; Chairul Chairul; Anthoni Agustien; Suwirmen Suwirmen; Rusnam Rusnam; Deswati Deswati
Jurnal Hilirisasi IPTEKS Vol 3 No 1 (2020)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.612 KB) | DOI: 10.25077/jhi.v3i1.347

Abstract

Lecturer and community based science and technology activities (IbDM) were carried out with the aim of disseminating technology for Gurame culture intensively in an effort to increase fisheries productivity in Limau Manis, Pauh Subdistrict, Padang City and increase the knowledge and skills of fish farmers (community) in Koto Tangah Village in the field of Gurame fish maintenance business in West Sumatra, so that they are expected to be able to carry out maintenance in a good / environmentally friendly manner. Besides this IbDM activities are also expected to be able to reduce operational costs in maintaining Nirwana Tilapia and in the end can increase the income of the farmers (community) of Limau Manis Village through the development of sustainable fish farming. To achieve optimal technology dissemination results, the main target of IbDM in Pauh Subdistrict is fish farmer groups and community leaders / leaders of Limau Manis Village who are expected to be able to motivate farmers to socialize the method of making alternative formula feeds for intensive development of Nirwana Tilapia aquaculture and environmentally friendly. The methods used in this activity are (1) counseling, with the main material: (a) providing knowledge of various kinds of raw materials that can be used for fish food, (b) providing knowledge about the preparation of artificial food formulas / compositions, ( c) provide knowledge about the process of making artificial food (d) increase public knowledge about how to store pellets that are good to be more durable and (e) provision of material on testing the quality of feed, both physically, chemically and biologically; and (2) Intensive demonstration of making alternative pellet feeds for Nirwana Tilapia aquaculture. From the results of the IbDM activity, it was found that the knowledge and skills of farmer groups in Limau Manis Sub-district, Kec. Pauh, Padang before the IbDM activities were carried out was still very low. However, the motivation or desire of the community of IbDM partner farmer groups to carry out the culture or maintenance of fish is very large. This can be seen from the desire of the IbDM partner farmer group to master the technology of making fish feed from the results of leaf waste and taro stems which are processed into flour relatively high. Furthermore, the results of IbDM activities in the community can increase the knowledge and skills of the community in finding alternative feed for fish feed so that culture activities can run well in IbDM partner areas. In order for aquaculture to run well in partner areas of IbDM, it is necessary to carry out IbDM activities continuously on the techniques of making alternative fish pellets and efficient and effective fish-raising techniques. Besides that, it needs financial support or credit by the authorities to the community or fish farmers for capital to develop their businesses.
DISEMINASI TEKNOLOGI FORMULATED DIET ALTERNATIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS BUDIDAYA IKAN NILA INTENSIF DI KOTA PADANG Efrizal Efrizal; Nurmiati Nurmiati; Chairul Chairul; Anthoni Agustien; Zuhri Syam; Suwirmen Suwirmen; Rusnam Rusnam; Deswati Deswati
Jurnal Hilirisasi IPTEKS Vol 4 No 4 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i4.534

Abstract

Fish cultivators generally experience difficulties providing quality artificial feed (pellets) due to a lack of knowledge and skills in processing technology and making alternative feeds. Besides that, the costs incurred for feed procurement are relatively large, reaching 70 - 80% of the total production costs. However, the provision of feed is often an obstacle because, in addition to the increasingly high price, the quality of available feed does not always match the nutritional needs needed by Fish. The provision of quality feed for intensively cultivated tilapia dramatically affects the resulting growth and productivity. The higher the quality of the feed, the production will also increase. One of the technologies that can be applied to overcome this problem is the manufacture of alternative formulated diets using local raw materials. The method used in this service activity is the transfer of knowledge presented in the socialization activities in the form of 1) introduction to the types of alternative fish feed ingredients sourced from the area, (2) preparation of artificial tilapia feed formulations, (3) manufacturing technology artificial feed (pellets) and 4) monitoring and assistance. The socialization was carried out in the form of a lecture and was followed by a question and answer session. The results of farmer activities recognize the types of local feed that can be used as fish feed, know the preparation of feed formulations, and alternative artificial feed manufacturing technologies. The knowledge and skills possessed by fish cultivators are expected to overcome the difficulties of fish farmers in providing feed so that fish production and cultivation business increase.
AMOBILISASI SEL Bacillus licheniformis KA-08 DALAM MENGHASILKAN KERATINASE TERMOSTABIL Anthoni Agustien
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 1 (2011): December 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.42 KB) | DOI: 10.23869/185

Abstract

Isolate local Bacillus licheniformis KA-08 known extracellular thermostable keratinase producers. Scale up of thermostable keratinase production can be with cells immobilized. The objective of the research is to thermostable keratinase production of B. licheniformis KA-08 cells immobilization. Thermostable keratinase activities were determined with modification of Brandelli and Riffel method. Protein concentration of enzyme determined with Lowry method. Immobilization of cells by Ca-alginate matrix with Adinarayana method, alginate concentration and amount of alginate bead effects with Beshay method. The result extracellular thermostable keratinase of B. licheniformis KA-08 cells immobilized was maximum produced at 12 times incubation with activity as 9.25 U/mg. Three percent alginate has optimum activity. Three hundred alginate beads has optimum activity. Cells immobilized of B. licheniformis KA-08 has scale up of thermostable keratinase activity at 2 times than free cells. Thermostable keratinase produced by cell immobilized was nine cycles.
The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Syukria Ikhsan Zam; Anthoni Agustien; Syamsuardi Syamsuardi; Akmal Djamaan; Irfan Mustafa
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
Antibacterial Test of Ziziphus spina-christi (L.) Desf. Leaves Extract Againstgram-Positive Bacteria Feskaharny Alamsjah; Anthoni Agustien; Fadhila Cantika
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 24 No. 02 (2023): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol24-iss02/278

Abstract

Ziziphus spina-christi (L.) Desf. is a member of the Rhamnaceae family, which has efficacy values as herbal plants used in traditional medicines. The spread of infectious diseases in humans has increased in recent years, raising public awareness of the importance of using traditional medicine as the first line of prevention against infectious diseases. This research aimed to determine the antibacterial activity of fresh extract, infusion, and ethanol extract and to determine the greatest inhibition zone of Z. spina-christi leaves against Gram-positive bacteria (B. cereus, B. subtilis, S. aureus, and S. epidermidis). This research used the survey method with a purposive sampling technique. The results showed that fresh extract, infusion, and ethanol extract of the Z. spina-christi leaves had antibacterial activity against Gram-positive bacteria (B. cereus, B. subtilis, S. aureus, and S. epidermidis) and the greatest inhibition zone was obtained from the ethanol extract with a strong inhibition category.
Co-Authors - Jumawita . Mansyurdin Aghni Aznia Akmal Djamaan Alviche, Lola Armaleni Armaleni Ayu Putri Ningsih Chairul Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dewi Yudiana Shinta Dianty Putri Dina Wahyuna Djong Hon Tjong Dwitaviani, Rima Efrizal Efrizal Efrizal Efrizal Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Herwita Idris Hesti Dwi Marcellinna Ikel Fitri Yanis Ilsa Septia Putri Irdawati Irdawati Irfan Mustafa Jetty Nurhajati Kurniadi Ilham Kustiasih Lestari Linar Z. Udin Lola Alviche Marlina Marlina Melisa Ainil Fajri Mira Rosnawita Muhammad Diki Juliandi Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Periadnadi Periadnadi Pingkan Aditiawati PINGKAN ADITIAWATI Putri, Silvy Rizka Rahmadani Eka Pitri Rahmi Adila Rahmi Inayati Riesca Salsabilah Rahmayati Riski Budi Yani RR. Ella Evrita Hestiandari Rusnam Rusnam Sophia, Anggun Suwirmen, Suwirmen Syafrita, Assyifa Syamsuardi Syamsuardi Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Triyatdipa, Haqil Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Wiwik Handayani Yetria Rilda Yetti Marlida Yurike Nova Edlin Zakaria, Indra Junadi Zovia, Miftahul Zozy Aneloi Noli Zuhri Syam Zuhri Syam