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All Journal HAYATI Journal of Biosciences Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL AGRONIDA JURNAL KESEHATAN PERINTIS Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Zona Bioscientist : Jurnal Ilmiah Biologi Agroradix : Jurnal Ilmu Pertanian Berkala Penelitian Hayati Journal of Pharmaceutical and Sciences. Organisms: Journal of Biosciences Bioeksperimen Biodiversitas Journal of Biological Diversity
Anthoni Agustien
Department Of Biology, Faculty Of Mathematics And Natural Sciences Universitas Andalas
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Species Diversity of Genus Aspergillus, Endophytic Fungal Isolated From Mangrove Ceriops tagal and their Antibiotic Potential Sophia, Anggun; Agustien, Anthoni; Chairul; Syamsuardi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1174-1184

Abstract

The rapid increase in antimicrobial resistance has encouraged development of new natural and urgent strategies to fight drug-resistant pathogens, one of which is using endophytic fungi. Endophytic fungi found in Ceriops tagal mangrove plant, which can adapt to extreme salinity environments of up to 60 ppt. Endophytic fungi are isolated from leaves, branch, and roots. Each isolate was identified morphologically and cultured in potato dextrose broth media for 21 days. After incubation, liquid culture was extracted with ethyl acetate and evaporated to obtain a thick extract. The Kirby Bauer method was used to evaluate the antibiotic potential of each endophytic fungal extract against pathogenic microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Concentration used was 5%, antibiotics used as positive controls were chloramphenicol (30μg/disk), nystatin (100 IU/disk), as negative controls DMSO. Three endophytic fungi were isolated from roots (ECT7, ECT 8, ECT 10), two from leaves (ECT 26, ECT 28) and six from branches (ECT 31, ECT 47, ECT 48, ECT 51, ECT 55 and ECT 85). Fungal isolates ECT10 has been proven to effectively exhibit strong antibiotic activity against microorganisms E. coli, S. aureus, and C. albicans, with inhibition zones measuring 13.0 mm, 11.8 mm, and 11.6 mm, respectively.
Growth Curve and Antibacterial Activity Test of Endophytic Bacteria Isolates 1 (IBE1) from Labu Koteka (Lagenaria siceraria) Against Escherochoa coli, Pseudomonas aeruginosa and MRSA Putri, Silvy Rizka; Djamaan, Akmal; Agustien, Anthoni
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8506

Abstract

The rising incidence of antibiotic-resistant bacteria presents a considerable global health threat, requiring the immediate creation of new antimicrobial drugs. A possible strategy involves investigating antibiotics sourced from endophytic bacteria, which are microorganisms that inhabit plant tissues symbiotically. This research is to assess the inhibitory efficacy of endophytic bacteria derived from Lagenaria sicerari against, Escherichia coli, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus (MRSA). The identified endophytic bacteria were cultivated in a fermentation medium to synthesize antibiotics, and their growth curves were examined. Subsequently, antibacterial activity assays were performed against the three harmful bacteria, measuring the sizes of the clear inhibitory zones. The results indicated that Isolated Bacteria Endophytic (IBE) 1 attained optimal inoculum levels at 24 hours and sustained the stationary phase from 24 to 36 hours. The isolates exhibited optimum fermentation on a medium utilizing glucose as the carbon source, with a carbon-to-nitrogen ratio of 5. IBE 1 had the most effective antibacterial action against P. aeruginosa, with a moderate inhibition characterized by clear zone widths of 10.80 mm. The findings indicate that endophytic bacteria from Lagenaria siceraria may serve as promising sources of new antimicrobial drugs, especially against Pseudomonas aeruginosa.
A Analysis of Ecoenzyme Characteristics from Combination of Fruit Peel Waste Triyatdipa, Haqil; Agustien, Anthoni; Marlina, Marlina
Biospecies Vol. 18 No. 2 (2025): Juli 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i2.44214

Abstract

Indonesia encounters many significant challenges in organic waste management, with 37.87% of the total 38.7 million tonnes of national waste (in 2024) unmanaged. Organic waste, such as fruit peels, which are often dumped into landfill, contributes to greenhouse gas emissions and environmental pollution. Ecoenzyme, a fermentation product of organic waste (1:3:10 ratio of sugar, substrate, water), is emerging as an innovative solution for waste conversion into value-added products. Besides reducing waste volume, ecoenzymes produce ozone (O₃) that suppresses the greenhouse effect, antimicrobial acetic acid, and natural cleaning compounds. Fruit peels such as orange, banana, papaya, watermelon and pineapple are potential substrates due to their cellulose and pectin content, but the characteristics of ecoenzyme depend on the interaction of chemical (pH, Total Acid Titration/TTA) and biological (Lactic Acid Bacteria/BAL, yeast) parameters. Previous studies focused on ecoenzymes based on one type of fruit peel, while the potential of combinations of various fruit peels has not been explored holistically. This study aims to analyse the characteristics of fruit peel combination ecoenzymes through evaluation of chemical (pH, TTA) and microbiological (LAB population, yeast) parameters. The results are expected to provide scientific guidance for the production of high-quality ecoenzymes while supporting sustainable organic waste reduction strategies.
The Potential of Bacillus spp. In Green Biosynthesis of Zinc Oxide Nanoparticles Alviche, Lola; Agustien, Anthoni; Djamaan, Akmal
Majalah Kedokteran Andalas Vol. 46 No. 3 (2023): Supplementary July 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i5.p818-835.2023

Abstract

Nanopartikel yang dihasilkan melalui metode Green biosintesis berpotensi untuk diterapkan pada dunis medis dan farmasi. Penelitian ini menggunakan senyawa metabolit yang dihasilkan isolat Bacillus spp untuk mereduksi zink sulfat menjadi nanopartikel zink oksida. Berdasarkan hasil pengukuran spetrofotometri UV-Vis panjang gelombang optimum koloid nanopartikel yang dihasilkan dari proses Green biosintesis isolate BES 6A dengan penambahan zink sulfat sebanyak 0,2 g/ml yaitu pada 366 nm. Adapun gugus fungsi Zn-O berdasarkan pengukuran FTIR terbaca pada panjang puncak gelombang 438 dan 530 cm-1. Bentuk kristal yang dihasilkan yaitu heksagonal dengan puncak 2Ɵ yang terdeteksi yaitu 31,82°; 34,33°; 36,49°; 47,56°; 57,16°; 63,20°; 66,76°; dan 68,99° yang menandakan kehadiran nanopartikel zink oksida berdasarkan referensi ICDD 00-001-1136. Ukuran nanopartikel yang dihasilkan yaitu 30 – 47 nm.
Uji Antagonis Bacillus subtilis ATTC 6633 dan Trichoderma harzianum terhadap Pertumbuhan Magnaphorte oryzae pada Benih Padi Anak Daro dengan Variasi Lama Perendaman Alamsjah, Feskaharny; Noli, Zozy Aneloi; Rahmayati, Riesca Salsabilah; Suwirmen, Suwirmen; Agustien, Anthoni; Ilham, Kurniadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9468

Abstract

Magnaphorte oryzae is a pathogenic fungus that causes blast disease in rice plants. This disease can cause a decrease in rice production. Therefore, it is necessary to control the disease, one of which is by administering microbes that are antagonistic to Magnaphorte oryzae. This research aims to determine the percentage of inhibitory power of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the growth of Magnaphorte oryzae with varying soaking times of 24, 48 and 72 hours on the Anak Daro rice variety. This research carried out viability tests for Bacillus subtilis ATTC 6633 and Trichoderma harzianum, as well as antagonist tests using the dual culture method. The results showed that the growth inhibition of Magnaphorte oryzae by Bacillus subtilis ATTC 6633 for a soaking period of 24 hours was 28.48% in the weak category, 48 hours was 32.27% and 72 hours was 31.01% in the medium category. Inhibition of Magnaphorte oryzae by Trichoderma harzianum for a soaking period of 24 hours was 50.63%, 48 hours was 57.59%, and 72 hours was 56.32% in the high category.
Antagonistic activity of phyllosphere fungi isolated from medicinal plants against Colletotrichum sp. causing anthracnose in chilli (Capsicum annuum L.) Puspita, Ayumi Rizci; Alamsjah, Feskaharny; Mildawati, Mildawati; Agustien , Anthoni
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16072

Abstract

Anthracnose disease caused by the fungus Colletotrichum sp. is one of the main problems in chili cultivation (Capsicum annuum L.) and can cause significant yield losses. The intensive use of chemical pesticides in controlling this disease has a negative impact on the environment and health. This study aimed to evaluate the antagonistic potential of phyllosphere fungi isolated from five medicinal plants, namely Phaleria macrocarpa, Curcuma domestica, Kleinhovia hospita, Hibiscus rosa-sinensis, and Morus macroura, against the pathogen Colletotrichum sp. Isolation was performed from leaf surfaces, and a total of 7 phyllosphere fungal isolates were tested using a double culture method with three replicates. The results showed that all isolates were able to inhibit the growth of Colletotrichum sp. through mechanisms of competition for space and nutrients as well as antibiosis, with inhibition levels ranging from high to very high. Among the tested isolates, Trichoderma sp. exhibited the highest inhibitory activity with an inhibition percentage of 92.78%, categorized as very high. Macroscopic and microscopic characterization supported the identification of several isolates belonging to the genera Trichoderma. This study concluded that phyllosphere ungi from medicinal plants have the potential as environmentally friendly biological control agents in combating anthracnose disease in chili plants.
Relationship between Proximate Characteristics and pH Value with Total Lactic Acid Bacteria in Etawa Goat Milk Annisa Farma; Djong Hon Tjong; Endang Purwati; Anthoni Agustien; Annisa Khaira
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.12044

Abstract

Etawa goat milk is a highly nutritious food source with strong potential to be developed as a functional food. Its nutritional components—such as protein, fat, and water content—along with the presence of lactic acid bacteria (LAB), contribute to its health benefits. This study aimed to analyze the proximate characteristics and pH value of Etawa goat milk and to evaluate their relationship with the total LAB count. Milk samples were collected from three locations in Padang City: Gunung Pangilun, Korong Gadang, and Padayo. Proximate analysis was conducted using standard methods from the Indonesian National Standardization Agency, while total LAB was determined using the Total Plate Count method on MRS Agar media. The results showed that moisture content ranged from 86.11–88.93%, protein content from 3.64–4.17%, fat content from 4.00–5.00%, and pH values from 6.70–7.00. The total LAB ranged from 30.66×10⁸ to 48.33×10⁸ CFU/mL, exceeding the minimum threshold required for probiotic sources. Samples with higher protein and fat content tended to have higher LAB counts. In addition, high moisture content and near-neutral pH supported microbial growth. Therefore, the proximate characteristics and pH value of Etawa goat milk are associated with LAB counts, indicating its potential as a natural probiotic source. The findings of this study provide scientific evidence for the utilization of Etawa goat milk as a natural probiotic product and may contribute to the development of dairy-based functional foods and community nutrition programs.
SKRINING BAKTERI PENGHASIL POLI(3-HIDROKSIBUTIRAT) P(3HB) DARI SAMPEL IKAN KEMBUNG (Rastrelliger sp.) Inayati Rahmi; Anthoni Agustien; Akmal Djamaan
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.5262

Abstract

Bioplastic P(3HB) is a plastic that can be decomposed by decomposing microorganisms. Bacteria can produce P(3HB) in conditions of low nutrition and high carbon sources accumulated in cells as energy reserves. Isolation of bacteria-producing bioplastic P(3HB) was carried out in mackerel fish's gills and intestines, allowing direct contact with polluted waters. This research aimed to determine the presence of P(3HB) bioplastic-producing bacteria in mackerel fish samples and to screen for P(3HB) bioplastic-producing bacteria. The stages of the research were the isolation of P(3HB) producing bacteria from the gills and intestines of mackerel fish, screening of P(3HB) bioplastic-producing bacteria by using Nile Blue A staining, and molecular identification of bioplastic-producing bacteria using 16S rRNA gene. The results of bacterial isolation in the gills and intestines of mackerel obtained 10 bacterial isolates. P(3HB) screening found 1 bacterium showing orange fluorescence, namely IKE-1 isolate which was isolated from the gills of mackerel fish and included in the Gram-negative group with a monobacilli cell shape. Molecular identification of bacteria by using the 16S rRNA gene that isolate IKE-1 is Enterobacter roggenkampii. These results indicate that Enterobacter roggenkampii can produce P(3HB) which was confirmed by the screening results
Isolation of Thermophilic Bacterial from Garara Hot Spring as Antimicrobial Producer Asih, Enda Tarni; Agustien, Anthoni; Maliza, Rita
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/gs4ca451

Abstract

Antibiotics were essential medicines in Indonesia, and due to the high prevalence of antimicrobial resistance, new sources of antibiotic substances were urgently needed. One of the microorganisms capable of producing novel antibiotics was thermophilic bacteria. This study aims to isolate thermophilic bacteria from Garara Hot Spring that had potential as antibiotic producers. The results indicated that isolate TUA107 exhibited the strongest antimicrobial activity against Escherichia coli and Staphylococcus aureus, with inhibition zones of 6.56 mm and 7.70 mm, respectively. These findings suggested that molecular identification of thermophilic bacteria and metabolomics studies of potential antibiotic-producing isolates from Garara Hot Spring were needed to optimize their potential.
In Vitro Antagonistic Activity of Trichoderma atroviride and Trichoderma harzianum against Colletotrichum sp. and Fusarium sp. Isolated from Chili (Capsicum annuum L.) Ayumi Rizci Puspita; Feskaharny Alamsjah; Anthoni Agustien; Suwirmen Suwirmen; Anisa Rahman Rusiati
AGRORADIX : Jurnal Ilmu Pertanian Vol. 9 No. 2 (2026): Januari-Juli 2026
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v9i2.13076

Abstract

Fungal diseases remain a major constraint in chili cultivation, particularly anthracnose and Fusarium wilt. This study aimed to evaluate the in vitro biocontrol potential of Trichoderma atroviride and Trichoderma harzianum against Colletotrichum sp. and Fusarium oxysporum f. sp. capsici. The study was conducted experimentally using the dual culture method on Potato Dextrose Agar (PDA) medium with three replications for each treatment. Results showed that both Trichoderma isolates exhibited strong inhibitory activity against the tested pathogens. Trichoderma atroviride showed inhibition percentages of 92% against Colletotrichum sp. and 90% against Fusarium sp., while Trichoderma harzianum inhibited pathogen growth by 73.6% and 85%, respectively. The primary antagonistic mechanism observed was competition for space and nutrients, indicated by the rapid growth of Trichoderma colonies covering the Petri dishes. This study indicates that T. atroviride and T. harzianum have promising in vitro biocontrol potential against Colletotrichum sp. and Fusarium oxysporum f. sp. capsici.
Co-Authors - Jumawita Aghni Aznia Akmal Djamaan Alif Rahman Habibi Alviche, Lola Alya Rahmaditya Arfan Anggun Sophia Anggun Sophia Anisa Rahman Rusiati Annisa Farma Annisa Khaira Armaleni Armaleni Arthauly Yopita Sinurat Asih, Enda Tarni Ayu Putri Ningsih Ayumi Rizci Puspita Ayumi Rizci Puspita Ayumi Rizci Puspita Chairul Chairul Chairul Chairul Cici Wulandari Deasy Ovi Harsachatri Desma Yuni Deswati Deswati Devi Syafriyani Dianty Putri Dina Wahyuna Djong Hon Tjong Dwitaviani, Rima Efrizal Efrizal Efrizal Efrizal Endang Purwati RN Fadhila Cantika Fatriza, Ayu Septia Ferry Lismanto Syaiful Feskaharny Alamsjah Herwita Idris Hesti Dwi Marcellinna Ikel Fitri Yanis Ilham, Kurniadi Ilsa Septia Putri Irdawati Irdawati Irfan Mustafa Irfan Suliansyah Jetty Nurhajati Julia Veronika Larasati Kurniadi Ilham Kustiasih Lestari Linar Z. Udin Lola Alviche Maliza, Rita Mansyurdin Marlina Marlina Marlina Marlina Melisa Ainil Fajri Mellanie Alia Putri Mellanie Alia Putri Mifthahul Jannah Mifthahul Jannah Mildawati Mildawati Mira Rosnawita Mohammad Abrar Mohsin Rafique Mufidhatul Muqarramah Muhammad Diki Juliandi Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Novia Novia Nurhajati, Jetty Nurlaili Nurlaili Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Periadnadi Periadnadi Pingkan Aditiawati PINGKAN ADITIAWATI Puspita, Ayumi Rizci Putri, Silvy Rizka Rahmadani Eka Pitri Rahmayati, Riesca Salsabilah Rahmi Adila Rahmi Inayati Riesca Salsabilah Rahmayati Rifda Hayati Riski Budi Yani RR. Ella Evrita Hestiandari Rusnam Rusnam Shinta, Dewi Yudiana Suwirmen, Suwirmen Syafrita, Assyifa Syamsuardi Syamsuardi Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Triyatdipa, Haqil Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Wiwik Handayani Yetria Rilda Yetti Marlida Yetty Marlida Yorasakhi Ananta Yuliandini Pangestika Yurike Nova Edlin Zakaria, Indra Junadi Zovia, Miftahul Zozy Aneloi Noli Zuhri Syam Zuhri Syam Zuhri Syam