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All Journal HAYATI Journal of Biosciences Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Best Journal (Biology Education, Sains and Technology) Jurnal Zona Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati
Anthoni Agustien
Universitas Andalas
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Optimasi Variasi Sumber Karbon dan Nitrogen Isolat Bakteri Endofitik dari Tanaman Bruguiera gymnorrhiza dalam Menghasilkan Crude Enzim Protease Agustien, Anthoni; Marlida, Yetti; Zovia, Miftahul
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 2 (2024): September 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i2.9705

Abstract

Enzim protease merupakan salah satu enzim komersial yang memiliki berbagai manfaat dan aplikasi pada bidang industri. Enzim protease dapat dihasilkan oleh mikroorganisme dan dapat dilakukan optimasi untuk menghasilkan aktivitas enzim yang optimum. Tujuan penelitian adalah untuk mengetahui jenis sumber karbon dan sumber nitrogen terbaik dalam menghasilkan enzim protease. Penelitian ini dilakukan dengan metode eksperimen menggunakan beberapa variasi jenis sumber karbon dan nitrogen untuk melihat aktivitas enzim protease melalui ekstrak kasar enzim (crude enzyme extract) yang dihasilkan. Hasil didapatkan dari  penelitian ini adalah sumber karbon yang terbaik berupa maltosa, sedangkan sumber nitrogen terbaik merupakan kalium nitrat (KNO3) konsentrasi 1% (v/v), dengan aktivitas protease sebesar 1,136 U/mL
Optimasi Enzim Isolat Bakteri Endofitik EUA-136 dari Sonneratia Sp. Penghasil Protease Agustien, Anthoni; Dwitaviani, Rima; Marlida, Yetti
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 1 (2024): Juni 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i1.9405

Abstract

Enzim protease memiliki kemampuan untuk memecah protein menjadi oligopeptida dan asam amino. Enzim yang dihasilkan oleh bakteri endofitik dapat diproduksi dalam skala besar tanpa perlu merusak atau menebang tanaman inangnya. Penelitian ini bertujuan untuk mengetahui suhu, pH, dan waktu inkubasi optimum terhadap isolat bakteri endofitik EUA-136 dalam meningkatkan aktivitas enzim protease. Penelitian ini merupakan penelitian eksperimen dengan menggunakan Response Surface Methodology (RSM) tipe rancangan Central Composite Design (CCD) pada software Design Expert 13. Hasil dari penelitian ini didapatkan aktivitas protease dari isolat bakteri endofitik EUA-136 optimum pada suhu 30 ºC, pH 8 dalam waktu inkubasi 15 menit. Keywords: optimasi, protease, bakteri endofitik, response surface methodology
Optimasi Sumber Karbon Dan Nitrogen Isolat Bakteri Termofilik TUA-504 Penghasil Protease Syafrita, Assyifa; Agustien, Anthoni; Marlida, Yetti
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 1 (2024): Juni 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i1.9708

Abstract

Protease merupakan enzim yang paling banyak dimanfaatkan diberbagai industri seperti industri kesehatan, farmasi, deterjen, makanan, dan tekstil. Protease termasuk golongan enzim hidrolase yang mampu memecah protein menjadi oligopeptida pendek atau asam amino. Enzim mudah terdenaturasi pada suhu tinggi. Sedangkan dalam industri, protease lebih banyak diaplikasikan pada suhu tinggi. Sehingga diperlukan enzim yang tahan terhadap suhu tinggi (termostabil). Enzim termostabil yang dihasilkan oleh bakteri termofilik sangat dibutuhkan dalam bidang industri karena kemampuannya yang tahan terhadap suhu tinggi. Tujuan dari penelitian ini untuk mengetahui sumber karbon dan sumber nitrogen optimum terhadap isolat bakteri termofilik TUA-504 produksi protease. Metode penelitian dilakukan secara eksperimen dengan menggunakan variasi sumber karbon dan sumber nitrogen konsentrasi 1% untuk mendapatkan kondisi optimum dari aktivitas protease. Hasil dari penelitian ini didapatkan kondisi optimum isolat bakteri termofilik TUA-504 dalam memproduksi protease yaitu pada sumber karbon glukosa dan sumber nitrogen NaNO3
Optimization of Protease Activity of Endophytic Bacteria EUA-136 and EUA-139 from Bruguiera gymnorrhiza Using Response Surface Methodology Agustien, Anthoni; Zovia, Miftahul; Dwitaviani, Rima; Marlida, Yetti
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.426-435

Abstract

Protease is a vital enzyme used in industries such as detergents, pharmaceuticals, and animal feed, with a growing demand in the enzyme market. Endophytic microorganisms can produce stable proteases with a rapid synthesis process. This study optimized conditions of temperature, pH, salinity, agitation, and nutrient sources for protease production by EUA-136 and EUA-139 bacterial isolates. The research used Response Surface Methodology (RSM) with a Central Composite Design (CCD) in Design Expert Software 13.1 to identify optimal conditions and the bacterial isolates. The optimum conditions for the EUA-136 bacterial isolate to produce protease were 3% inoculum at 30 ºC, pH 7, 28.5 ppt salinity, and 150 rpm agitation. For the EUA-139 bacterial isolate, the optimum conditions were a carbon source of 1% (v/v) maltose, a nitrogen source of 1% (v/v) KNO3, casein as the inducer, and an inoculum concentration of 7.5% (v/v). Molecular identification of isolates EUA-136 and EUA-139 revealed similarities to Bacillus cereus strain 3TC-3 and Bacillus paramycoides 3665, respectively.
Species Diversity of Genus Aspergillus, Endophytic Fungal Isolated From Mangrove Ceriops tagal and their Antibiotic Potential Sophia, Anggun; Agustien, Anthoni; Chairul; Syamsuardi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1174-1184

Abstract

The rapid increase in antimicrobial resistance has encouraged development of new natural and urgent strategies to fight drug-resistant pathogens, one of which is using endophytic fungi. Endophytic fungi found in Ceriops tagal mangrove plant, which can adapt to extreme salinity environments of up to 60 ppt. Endophytic fungi are isolated from leaves, branch, and roots. Each isolate was identified morphologically and cultured in potato dextrose broth media for 21 days. After incubation, liquid culture was extracted with ethyl acetate and evaporated to obtain a thick extract. The Kirby Bauer method was used to evaluate the antibiotic potential of each endophytic fungal extract against pathogenic microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Concentration used was 5%, antibiotics used as positive controls were chloramphenicol (30μg/disk), nystatin (100 IU/disk), as negative controls DMSO. Three endophytic fungi were isolated from roots (ECT7, ECT 8, ECT 10), two from leaves (ECT 26, ECT 28) and six from branches (ECT 31, ECT 47, ECT 48, ECT 51, ECT 55 and ECT 85). Fungal isolates ECT10 has been proven to effectively exhibit strong antibiotic activity against microorganisms E. coli, S. aureus, and C. albicans, with inhibition zones measuring 13.0 mm, 11.8 mm, and 11.6 mm, respectively.
Growth Curve and Antibacterial Activity Test of Endophytic Bacteria Isolates 1 (IBE1) from Labu Koteka (Lagenaria siceraria) Against Escherochoa coli, Pseudomonas aeruginosa and MRSA Putri, Silvy Rizka; Djamaan, Akmal; Agustien, Anthoni
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8506

Abstract

The rising incidence of antibiotic-resistant bacteria presents a considerable global health threat, requiring the immediate creation of new antimicrobial drugs. A possible strategy involves investigating antibiotics sourced from endophytic bacteria, which are microorganisms that inhabit plant tissues symbiotically. This research is to assess the inhibitory efficacy of endophytic bacteria derived from Lagenaria sicerari against, Escherichia coli, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus (MRSA). The identified endophytic bacteria were cultivated in a fermentation medium to synthesize antibiotics, and their growth curves were examined. Subsequently, antibacterial activity assays were performed against the three harmful bacteria, measuring the sizes of the clear inhibitory zones. The results indicated that Isolated Bacteria Endophytic (IBE) 1 attained optimal inoculum levels at 24 hours and sustained the stationary phase from 24 to 36 hours. The isolates exhibited optimum fermentation on a medium utilizing glucose as the carbon source, with a carbon-to-nitrogen ratio of 5. IBE 1 had the most effective antibacterial action against P. aeruginosa, with a moderate inhibition characterized by clear zone widths of 10.80 mm. The findings indicate that endophytic bacteria from Lagenaria siceraria may serve as promising sources of new antimicrobial drugs, especially against Pseudomonas aeruginosa.
A Analysis of Ecoenzyme Characteristics from Combination of Fruit Peel Waste Triyatdipa, Haqil; Agustien, Anthoni; Marlina, Marlina
Biospecies Vol. 18 No. 2 (2025): Juli 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i2.44214

Abstract

Indonesia encounters many significant challenges in organic waste management, with 37.87% of the total 38.7 million tonnes of national waste (in 2024) unmanaged. Organic waste, such as fruit peels, which are often dumped into landfill, contributes to greenhouse gas emissions and environmental pollution. Ecoenzyme, a fermentation product of organic waste (1:3:10 ratio of sugar, substrate, water), is emerging as an innovative solution for waste conversion into value-added products. Besides reducing waste volume, ecoenzymes produce ozone (O₃) that suppresses the greenhouse effect, antimicrobial acetic acid, and natural cleaning compounds. Fruit peels such as orange, banana, papaya, watermelon and pineapple are potential substrates due to their cellulose and pectin content, but the characteristics of ecoenzyme depend on the interaction of chemical (pH, Total Acid Titration/TTA) and biological (Lactic Acid Bacteria/BAL, yeast) parameters. Previous studies focused on ecoenzymes based on one type of fruit peel, while the potential of combinations of various fruit peels has not been explored holistically. This study aims to analyse the characteristics of fruit peel combination ecoenzymes through evaluation of chemical (pH, TTA) and microbiological (LAB population, yeast) parameters. The results are expected to provide scientific guidance for the production of high-quality ecoenzymes while supporting sustainable organic waste reduction strategies.
The Potential of Bacillus spp. In Green Biosynthesis of Zinc Oxide Nanoparticles Alviche, Lola; Agustien, Anthoni; Djamaan, Akmal
Majalah Kedokteran Andalas Vol. 46 No. 3 (2023): Supplementary July 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i5.p818-835.2023

Abstract

Nanopartikel yang dihasilkan melalui metode Green biosintesis berpotensi untuk diterapkan pada dunis medis dan farmasi. Penelitian ini menggunakan senyawa metabolit yang dihasilkan isolat Bacillus spp untuk mereduksi zink sulfat menjadi nanopartikel zink oksida. Berdasarkan hasil pengukuran spetrofotometri UV-Vis panjang gelombang optimum koloid nanopartikel yang dihasilkan dari proses Green biosintesis isolate BES 6A dengan penambahan zink sulfat sebanyak 0,2 g/ml yaitu pada 366 nm. Adapun gugus fungsi Zn-O berdasarkan pengukuran FTIR terbaca pada panjang puncak gelombang 438 dan 530 cm-1. Bentuk kristal yang dihasilkan yaitu heksagonal dengan puncak 2Ɵ yang terdeteksi yaitu 31,82°; 34,33°; 36,49°; 47,56°; 57,16°; 63,20°; 66,76°; dan 68,99° yang menandakan kehadiran nanopartikel zink oksida berdasarkan referensi ICDD 00-001-1136. Ukuran nanopartikel yang dihasilkan yaitu 30 – 47 nm.
Uji Antagonis Bacillus subtilis ATTC 6633 dan Trichoderma harzianum terhadap Pertumbuhan Magnaphorte oryzae pada Benih Padi Anak Daro dengan Variasi Lama Perendaman Alamsjah, Feskaharny; Noli, Zozy Aneloi; Rahmayati, Riesca Salsabilah; Suwirmen, Suwirmen; Agustien, Anthoni; Ilham, Kurniadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9468

Abstract

Magnaphorte oryzae is a pathogenic fungus that causes blast disease in rice plants. This disease can cause a decrease in rice production. Therefore, it is necessary to control the disease, one of which is by administering microbes that are antagonistic to Magnaphorte oryzae. This research aims to determine the percentage of inhibitory power of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the growth of Magnaphorte oryzae with varying soaking times of 24, 48 and 72 hours on the Anak Daro rice variety. This research carried out viability tests for Bacillus subtilis ATTC 6633 and Trichoderma harzianum, as well as antagonist tests using the dual culture method. The results showed that the growth inhibition of Magnaphorte oryzae by Bacillus subtilis ATTC 6633 for a soaking period of 24 hours was 28.48% in the weak category, 48 hours was 32.27% and 72 hours was 31.01% in the medium category. Inhibition of Magnaphorte oryzae by Trichoderma harzianum for a soaking period of 24 hours was 50.63%, 48 hours was 57.59%, and 72 hours was 56.32% in the high category.
SKRINING BAKTERI PENGHASIL POLI(3-HIDROKSIBUTIRAT) P(3HB) DARI SAMPEL IKAN KEMBUNG (Rastrelliger sp.) Rahmi, Inayati; Agustien, Anthoni; Djamaan, Akmal
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.5262

Abstract

Bioplastic P(3HB) is a plastic that can be decomposed by decomposing microorganisms. Bacteria can produce P(3HB) in conditions of low nutrition and high carbon sources accumulated in cells as energy reserves. Isolation of bacteria-producing bioplastic P(3HB) was carried out in mackerel fish's gills and intestines, allowing direct contact with polluted waters. This research aimed to determine the presence of P(3HB) bioplastic-producing bacteria in mackerel fish samples and to screen for P(3HB) bioplastic-producing bacteria. The stages of the research were the isolation of P(3HB) producing bacteria from the gills and intestines of mackerel fish, screening of P(3HB) bioplastic-producing bacteria by using Nile Blue A staining, and molecular identification of bioplastic-producing bacteria using 16S rRNA gene. The results of bacterial isolation in the gills and intestines of mackerel obtained 10 bacterial isolates. P(3HB) screening found 1 bacterium showing orange fluorescence, namely IKE-1 isolate which was isolated from the gills of mackerel fish and included in the Gram-negative group with a monobacilli cell shape. Molecular identification of bacteria by using the 16S rRNA gene that isolate IKE-1 is Enterobacter roggenkampii. These results indicate that Enterobacter roggenkampii can produce P(3HB) which was confirmed by the screening results
Co-Authors - Jumawita . Mansyurdin Aghni Aznia Akmal Djamaan Alviche, Lola Anggun Sophia Armaleni Armaleni Ayu Putri Ningsih Chairul Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dewi Yudiana Shinta Dianty Putri Dina Wahyuna Djong Hon Tjong Dwitaviani, Rima Efrizal Efrizal Efrizal Efrizal Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Herwita Idris Hesti Dwi Marcellinna Ikel Fitri Yanis Ilham, Kurniadi Ilsa Septia Putri Irdawati Irdawati Irfan Mustafa Jetty Nurhajati Kurniadi Ilham Kustiasih Lestari Linar Z. Udin Lola Alviche Marlina Marlina Melisa Ainil Fajri Mira Rosnawita Muhammad Diki Juliandi Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Periadnadi Periadnadi PINGKAN ADITIAWATI Pingkan Aditiawati Putri, Silvy Rizka Rahmadani Eka Pitri Rahmayati, Riesca Salsabilah Rahmi Adila Rahmi, Inayati Riesca Salsabilah Rahmayati Riski Budi Yani RR. Ella Evrita Hestiandari Rusnam Rusnam Suwirmen, Suwirmen Syafrita, Assyifa Syamsuardi Syamsuardi Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Triyatdipa, Haqil Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Wiwik Handayani Yetria Rilda Yetti Marlida Yurike Nova Edlin Zakaria, Indra Junadi Zovia, Miftahul Zozy Aneloi Noli Zuhri Syam Zuhri Syam