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Uji Antibakteri Ekstrak Rimpang Koenih Rimbo (Curcuma sumatrana Miq.) Tumbuhan Endemik Sumatra Barat terhadap Bakteri Gram Positif Feskaharny Alamsjah; Anthoni Agustien; Tri Widia Ningsih Alam
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7697

Koenih rimbo (Curcuma sumatrana Miq.) is a medicinal plant endemic to Sumatra from the Zingiberaceae family. Research on the antibacterial test of koenih rimbo rhizome extracts against Gram-positive bacteria has been conducted at the Basic Biology Laboratory, UPT. Basic and Central Laboratory, Chemical Laboratory of Natural Materials, UPT. Biological Resources, Andalas University,Padang. This study aims to determine the antibacterial activity of fresh extracts, infusions, and ethanol extracts and determine the largest inhibition zone of koenih rimbo rhizomes against several Gram-positive bacteria. The research used a survey method with a purposive sampling technique. The results showed that the fresh extract, infusa, and ethanol extract had antibacterial activity against Gram-positive bacteria (Staphylococcus aureus, S. epidermidis, Bacillus subtilis, and B. cereus. The most significant inhibition zone was obtained from the extract with ethanol solvent with a strong inhibition category.

The Potential of Bacillus spp. In Green Biosynthesis of Zinc Oxide Nanoparticles Lola Alviche; Anthoni Agustien; Akmal Djamaan
Majalah Kedokteran Andalas Vol 46, No 5 (2023): Supplementary July 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i5.p818-835.2023

Nanopartikel yang dihasilkan melalui metode Green biosintesis berpotensi untuk diterapkan pada dunis medis dan farmasi. Penelitian ini menggunakan senyawa metabolit yang dihasilkan isolat Bacillus spp untuk mereduksi zink sulfat menjadi nanopartikel zink oksida. Berdasarkan hasil pengukuran spetrofotometri UV-Vis panjang gelombang optimum koloid nanopartikel yang dihasilkan dari proses Green biosintesis isolate BES 6A dengan penambahan zink sulfat sebanyak 0,2 g/ml yaitu pada 366 nm. Adapun gugus fungsi Zn-O berdasarkan pengukuran FTIR terbaca pada panjang puncak gelombang 438 dan 530 cm-1. Bentuk kristal yang dihasilkan yaitu heksagonal dengan puncak 2Ɵ yang terdeteksi yaitu 31,82°; 34,33°; 36,49°; 47,56°; 57,16°; 63,20°; 66,76°; dan 68,99° yang menandakan kehadiran nanopartikel zink oksida berdasarkan referensi ICDD 00-001-1136. Ukuran nanopartikel yang dihasilkan yaitu 30 – 47 nm.

SKRINING BAKTERI PENGHASIL POLI(3-HIDROKSIBUTIRAT) P(3HB) DARI SAMPEL IKAN KEMBUNG (Rastrelliger sp.) Inayati Rahmi; Anthoni Agustien; Akmal Djamaan
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.5262

Bioplastic P(3HB) is a plastic that can be decomposed by decomposing microorganisms. Bacteria can produce P(3HB) in conditions of low nutrition and high carbon sources accumulated in cells as energy reserves. Isolation of bacteria-producing bioplastic P(3HB) was carried out in mackerel fish's gills and intestines, allowing direct contact with polluted waters. This research aimed to determine the presence of P(3HB) bioplastic-producing bacteria in mackerel fish samples and to screen for P(3HB) bioplastic-producing bacteria. The stages of the research were the isolation of P(3HB) producing bacteria from the gills and intestines of mackerel fish, screening of P(3HB) bioplastic-producing bacteria by using Nile Blue A staining, and molecular identification of bioplastic-producing bacteria using 16S rRNA gene. The results of bacterial isolation in the gills and intestines of mackerel obtained 10 bacterial isolates. P(3HB) screening found 1 bacterium showing orange fluorescence, namely IKE-1 isolate which was isolated from the gills of mackerel fish and included in the Gram-negative group with a monobacilli cell shape. Molecular identification of bacteria by using the 16S rRNA gene that isolate IKE-1 is Enterobacter roggenkampii. These results indicate that Enterobacter roggenkampii can produce P(3HB) which was confirmed by the screening results

Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms

Karaktersasi Bakteri Pengurai Plastik Sintetis Polipropilen dari Sampel Air Laut Studi Kasus dan Potensi Lingkungan Muhammad Diki Juliandi; Akmal Djamaan; Dewi Yudiana Shinta; Anthoni Agustien
Jurnal Zona Vol 8, No 1 (2024)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/zona.v8i1.113

The increase in the number of waste piles in Indonesia has reached 175,000 tons/day or the equivalent of 64 million tons/year. In the health sector, synthetic plastics are used as materials for making packaged medicine bottles and infusion bottles. Polypropylene synthetic plastics are very slow to degrade, making them a major problem in environmental pollution. This study aims to determine the type of bacteria and the ability of bacterial isolates to degrade polypropylene plastics. The research methods used include characterization of bacterial isolates macroscopicly, microscopicly, biochemical tests, then polypropylene synthetic plastic biodegradation tests were carried out during the incubation period of 1 week, 2 weeks, 3 weeks, and 4 weeks using an incubator shaker device. The results of this study obtained 4 bacterial isolates that can decompose polypropylene plastic from seawater samples in Padang City. The results of the isolation of polypropylene plastic bacteria from seawater samples in Padang City ILT-14 bacterial isolate based on macroscopic characteristics. and molecular identification was carried out in the LIPI biotechnology testing laboratory by the 16S rRNA gene deritimization method obtained polypropylene plastic scavenging bacterial species, namely: ILT-14 has similarities with Stenotropomonas Maltophilia. With a 30-day polypropylene plastic decomposer percentage of 10.8%. The difference in FTIR analysis was in the percentage value of carbon group transmission, and the aromatic group decreased. When compared to plastic before it was degraded and there was a decrease in percent. Microscopy Electron Scanning (SEM) Analysis of ILR-14 polypropylene plastic isolate of bacteria isolated is able to break down complex polymers into monomer forms

Pengendalian Athelia rolfsii Penyebab Busuk Pangkal Batang Pada Kacang Tanah Arachis hypogea. L Dengan Fungisida Nabati Dan Agensia Hayati (Review) Herwita Idris; Anthoni Agustien; Mansyurdin Mansyurdin
Jurnal AGROSAINS dan TEKNOLOGI Vol 8, No 2 (2023)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.8.2.87-93

Athelia rolfsii, Curzi adalah bentuk seksualitas dari spesies Sclerotium rolfsii, Sacc, salah satu spesies jamur patogen yang dapat menyebabkan beberapa penyakit mematikan pada tanaman seperti busuk batang, layu dan rebah kecambah. Jamur ini merupakan jamur tular tanah yang dapat bertahan lama dan membentuk sklerotia di dalam tanah. Biasanya serangan dari patogen A. rolfsii, Curzi, ini masyarakat petani cenderung mengendalikannya dengan pemakaian pestisida yang berbahan aktif kimia. Hasil monitoring pengendalian yang memakai fungisida sintetis dalam mengendalikan patogen menimbulkan dampak negatif, akibat dari residu pestisida pada produk pertanian, serta resistensi dan resurgensi penyakit. Oleh sebab itu perlu dicari pestisida sebagai pengendali yang tidak mempunyai dampak negatif. Diantaranya adalah pemakaian pestisida yang bahan aktif tanaman ataupun hayati. Dari hasil beberapa penelitian yang telah dilakukan, setiap bahan baku yang berbeda akan mempunyai daya hambat yang berbeda pula. Untuk itu akan diuraikan dibawah ini hasil-hasil penelitian sebagai pengendali A. rolfsii Cruzi dari golongan fungisida nabati dan agens hayati.ABSTRACTAthelia rolfsii, Curzi is a form of sexuality from the species Sclerotium rolfsii, Sacc, one of the pathogenic fungal species that can cause several deadly diseases in plants such as stem rot, wilting and drooping of sprouts. This fungus is a soil-borne fungus that can survive for a long time and form sclerotia in the soil. Usually the attack is from the pathogen A. rolfsii, Curzi, the farming community tends to control it by using pesticides with active chemical ingredients. The results of monitoring control using synthetic chemical fungicides in controlling pathogens have a negative impact, as a result of pesticide residues in agricultural products, as well as disease resistance and resurgence. Therefore it is necessary to look for pesticides as controllers that do not have a negative impact. Among them is the use of pesticides with active plant or biological ingredients. From the results of several studies that have been carried out, each different raw material will have a different inhibition power. For this reason, the results of research as controllers for A. rolfsii Cruzi will be described below from the class of botanical fungicides and biological agents.

Optimasi Variasi Sumber Karbon dan Nitrogen Isolat Bakteri Endofitik dari Tanaman Bruguiera gymnorrhiza dalam Menghasilkan Crude Enzim Protease Agustien, Anthoni; Marlida, Yetti; Zovia, Miftahul
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 2 (2024): September 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i2.9705

Enzim protease merupakan salah satu enzim komersial yang memiliki berbagai manfaat dan aplikasi pada bidang industri. Enzim protease dapat dihasilkan oleh mikroorganisme dan dapat dilakukan optimasi untuk menghasilkan aktivitas enzim yang optimum. Tujuan penelitian adalah untuk mengetahui jenis sumber karbon dan sumber nitrogen terbaik dalam menghasilkan enzim protease. Penelitian ini dilakukan dengan metode eksperimen menggunakan beberapa variasi jenis sumber karbon dan nitrogen untuk melihat aktivitas enzim protease melalui ekstrak kasar enzim (crude enzyme extract) yang dihasilkan. Hasil didapatkan dari penelitian ini adalah sumber karbon yang terbaik berupa maltosa, sedangkan sumber nitrogen terbaik merupakan kalium nitrat (KNO3) konsentrasi 1% (v/v), dengan aktivitas protease sebesar 1,136 U/mL

Optimasi Enzim Isolat Bakteri Endofitik EUA-136 dari Sonneratia Sp. Penghasil Protease Agustien, Anthoni; Dwitaviani, Rima; Marlida, Yetti
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 1 (2024): Juni 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i1.9405

Enzim protease memiliki kemampuan untuk memecah protein menjadi oligopeptida dan asam amino. Enzim yang dihasilkan oleh bakteri endofitik dapat diproduksi dalam skala besar tanpa perlu merusak atau menebang tanaman inangnya. Penelitian ini bertujuan untuk mengetahui suhu, pH, dan waktu inkubasi optimum terhadap isolat bakteri endofitik EUA-136 dalam meningkatkan aktivitas enzim protease. Penelitian ini merupakan penelitian eksperimen dengan menggunakan Response Surface Methodology (RSM) tipe rancangan Central Composite Design (CCD) pada software Design Expert 13. Hasil dari penelitian ini didapatkan aktivitas protease dari isolat bakteri endofitik EUA-136 optimum pada suhu 30 ºC, pH 8 dalam waktu inkubasi 15 menit. Keywords: optimasi, protease, bakteri endofitik, response surface methodology

Optimasi Sumber Karbon Dan Nitrogen Isolat Bakteri Termofilik TUA-504 Penghasil Protease Syafrita, Assyifa; Agustien, Anthoni; Marlida, Yetti
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 1 (2024): Juni 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i1.9708

Protease merupakan enzim yang paling banyak dimanfaatkan diberbagai industri seperti industri kesehatan, farmasi, deterjen, makanan, dan tekstil. Protease termasuk golongan enzim hidrolase yang mampu memecah protein menjadi oligopeptida pendek atau asam amino. Enzim mudah terdenaturasi pada suhu tinggi. Sedangkan dalam industri, protease lebih banyak diaplikasikan pada suhu tinggi. Sehingga diperlukan enzim yang tahan terhadap suhu tinggi (termostabil). Enzim termostabil yang dihasilkan oleh bakteri termofilik sangat dibutuhkan dalam bidang industri karena kemampuannya yang tahan terhadap suhu tinggi. Tujuan dari penelitian ini untuk mengetahui sumber karbon dan sumber nitrogen optimum terhadap isolat bakteri termofilik TUA-504 produksi protease. Metode penelitian dilakukan secara eksperimen dengan menggunakan variasi sumber karbon dan sumber nitrogen konsentrasi 1% untuk mendapatkan kondisi optimum dari aktivitas protease. Hasil dari penelitian ini didapatkan kondisi optimum isolat bakteri termofilik TUA-504 dalam memproduksi protease yaitu pada sumber karbon glukosa dan sumber nitrogen NaNO3

Uji Antagonis Bacillus subtilis ATTC 6633 dan Trichoderma harzianum terhadap Pertumbuhan Magnaphorte oryzae pada Benih Padi Anak Daro dengan Variasi Lama Perendaman Feskaharny Alamsjah; Zozy Aneloi Noli; Riesca Salsabilah Rahmayati; Suwirmen Suwirmen; Anthoni Agustien; Kurniadi Ilham
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9468

Magnaphorte oryzae is a pathogenic fungus that causes blast disease in rice plants. This disease can cause a decrease in rice production. Therefore, it is necessary to control the disease, one of which is by administering microbes that are antagonistic to Magnaphorte oryzae. This research aims to determine the percentage of inhibitory power of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the growth of Magnaphorte oryzae with varying soaking times of 24, 48 and 72 hours on the Anak Daro rice variety. This research carried out viability tests for Bacillus subtilis ATTC 6633 and Trichoderma harzianum, as well as antagonist tests using the dual culture method. The results showed that the growth inhibition of Magnaphorte oryzae by Bacillus subtilis ATTC 6633 for a soaking period of 24 hours was 28.48% in the weak category, 48 hours was 32.27% and 72 hours was 31.01% in the medium category. Inhibition of Magnaphorte oryzae by Trichoderma harzianum for a soaking period of 24 hours was 50.63%, 48 hours was 57.59%, and 72 hours was 56.32% in the high category.

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