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MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF Donax faba (BIVALVIA: DONACIDAE) OBTAINED FROM KUTANG BEACH, LAMONGAN, INDONESIA Ambarwati, Reni; Rachmadiarti, Fida; Fitrihidajati, Herlina; Purnomo, Tarzan; Rahayu, Dwi Anggorowati; Faizah, Ulfi
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.2143

Abstract

ARTICLE HIGLIGHTSThis study revealed 19 morphological variations of edible wedge clams, Donax faba (Bivalvia: Donacidae), from Kutang Beach, Lamongan, Indonesia, which has significant role as part of coastal ecosystem.This reseach also highlights the DNA barcoding of Donax faba based on COI gene (Cytochrome C Oxidase Subunit I).The average genetic distance of the research samples was 0.46%, while the value of this parameter between the research samples and the ingroup was found to be 1.51%. ABSTRACTDonax faba is a type of wedge clams with significant ecological and economic roles, as well as variations in color, pattern, and morphometric properties. Recently, a particular population of unidentified D. faba was reported from Kutang Beach, Lamongan, Indonesia. Therefore, this research aimed to assess the morphological variations and molecular characteristics of D. faba obtained from Kutang Beach based on COI gene. A total of 288 samples were collected during the lowest tide for morphological characterization of the color, pattern, and morphometrics of the shells. Additionally, molecular characterization was conducted based on the composition of nucleotide bases and amino acids of COI gene, genetic distance, as well as the relationships. The results showed that D. faba had 19 morphological variations, among which the most dominant type contained a whitish exterior with brown spots and a whitish purple interior. The average genetic distance of the samples was 0.46%, while the value was calculated as 1.51% between the samples and the ingroup. Automatic Barcode Gap Discovery (ABGD) analysis performed using a prior maximal distance of 0.001 showed the separation of these species into distinct categories.
SPECIES COMPOSITION OF INTERTIDAL BIVALVES IN PRENDUAN BEACH, MADURA ISLAND Nazihah, Fathimah Qonita; Ambarwati, Reni; Isnaningsih, Nur Rohmatin
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7831

Abstract

Prenduan Beach on the southern coast of Sumenep Regency, Madura Island, is a habitat for various species of bivalves that may be used further. The objective of our research is to document and examine the bivalvia population in Prenduan Beach. Sampling was carried out using the 5 x 5 m quadrat plot method with a 1 x 1 m subplot at three stations with different substrates. The species diversity index (Shannon-Wiener) ranged from 1.15 to 1.36 and was classified as low. Relative abundance (RA) ranged from 0.63 to 60.63%. This study collected 159 bivalvia specimens from seven families and 11 distinct species. The two species with the highest relative abundance were Anadara antiquata (60.68%) and Solen sp. (15.72%). Both species have the potential to be used as a source of seafood.
FURTHER STUDY ON TWO SPECIES OF LOACH FISHES (Cypriniformes: Nemacheilidae: Nemacheilus) BASED ON MORPHOLOGY AND MOLECULAR DATA Rahayu, Dwi Anggorowati; Kuntjoro, Sunu; Budijastuti, Widowati; Winarsih, Winarsih; Ambarwati, Reni; Nugroho, Endik Deni; Basith, Abdul; Kurniawan, Nia; Haryono, Haryono
BIOTROPIA Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1942

Abstract

The identities of two local loaches, Nemacheilus chrysolaimos (Valenciennes, 1846) and N. fasciatus (Valenciennes, 1846) from six rivers, were obtained through a comprehensive examination of their morphology and molecular characteristics in Biltar Regency, East Java, Indonesia. Therefore, this study identified Nemacheilus spp. from Blitar based on morphology and partial sequence of COI. The meristic data obtained for N. chrysolaimos included DII. 7–8 (dorsal fin), AI. 3–5 (anal fin), PI. 9 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). On the other hand, N. fasciatus exhibited the following meristic data, namely D II 7–8 (dorsal fin), AI. 6 (anal fin), PI. 9–10 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). A significant difference was observed in the morphometric characteristics of N. fasciatus across various sampling sites, as determined by the Kruskal-Wallis Test. Furthermore, the nucleotide base composition sequences of Nemacheilus spp. consisted of Thiamine (T), Cytosine (C), Adenine (A), and Guanine (G) with a mean of 29.565%, 32.023%, 23.88%, and 16.244%. Maximum Likelihood (ML) and Minimum Evolution (ME) phylogenetic analysis was also conducted using the Kimura 2 Parameter model to establish two major clades on Nemacheilus spp. and one out-group significantly different from the Nemacheilus spp. The results showed that these major clades exhibited a close relationship at 100% bootstrap support and were grouped under the genus Nemacheilus. The study on Nemacheilus spp. from the Blitar locality differentiated COI sequences between N. fasciatus and N. chrysolaimos. Additionally, N. chrysolaimos, as inferred from reference sequences, was identified as the ancestral species to N. chrysolaimos MZB 26540 and MZB 26539. ABGD analyses, employing a prior maximal distance of 0.025, also indicated the separation of these species into distinct partitions. The integration of morphology and genetic data for Nemacheilus spp. should provide valuable insights for future genetic population studies and conservation initiatives.
Morphological and Morphometric Study of Pila celebensis (Gastropoda: Ampullariidae) from Madura Island, Indonesia Hanuninda, Dewi; Ambarwati, Reni; Isnaningsih, Nur Rohmatin
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 2 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n2.p160-167

Abstract

Pila celebensis (family Ampullariidae) is a native freshwater snail species found in Asia, including Indonesia, and possesses significant ecological and economic importance. However, Pila populations face various threats due to the invasiveness of  Pomacea, as well as human activities. This study aimed to document the presence of P. celebensis on Madura Island and describe its morphological and morphometric characteristics. Samples were collected from four localities in Madura. All specimens had a similar morphological shell, including a spherical shell with a prominent brown color and dark brown spiral pattern, an oval to elongate lunar aperture, a thin or closed umbilicus, and a concentric operculum. However, morphometric size varied significantly by location. The largest individuals were found in the rice fields of South Poter Village, while the smallest were found in a stream in Nyalabuh Laok Village. This difference reveals that environmental factors, notably habitat conditions and food quality, have a significant impact on shell growth, with higher nutrition supporting larger sizes.Pila celebensis (family Ampullariidae) is a native freshwater snail species found in Asia, including Indonesia, and possesses significant ecological and economic importance. However, Pila populations face various threats due to the invasiveness of  Pomacea, as well as human activities. This study aimed to document the presence of P. celebensis on Madura Island and describe its morphological and morphometric characteristics. Samples were collected from four localities in Madura. All specimens had a similar morphological shell, including a spherical shell with a prominent brown color and dark brown spiral pattern, an oval to elongate lunar aperture, a thin or closed umbilicus, and a concentric operculum. However, morphometric size varied significantly by location. The largest individuals were found in the rice fields of South Poter Village, while the smallest were found in a stream in Nyalabuh Laok Village. This difference reveals that environmental factors, notably habitat conditions and food quality, have a significant impact on shell growth, with higher nutrition supporting larger sizes.
Pemodelan Building Information Modeling Dalam Tahap Pelaksanaan Konstruksi Pembangunan : STUDI KASUS: Gedung Fakultas Kedokteran Gigi Tahap II Universitas Brawijaya) ambarwati, reni
Sinteks : Jurnal Teknik Vol 14 No 2 (2025): Vol 14 No 2 (2025)
Publisher : Sekolah Tinggi Teknik Malang

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Pemodelan Building Information Modeling (BIM) telah menjadi bagian integral dari industri konstruksi, memberikan pendekatan terpadu untuk merencanakan, melaksanakan, dan mengelola proyek konstruksi. Studi ini bertujuan untuk menganalisis manfaat perhitungan volume menggunakan BIM Revit dalam tahap pelaksanaan konstruksi, dibandingkan dengan perhitungan volume konvensional, dengan fokus pada studi kasus Gedung Fakultas Kedokteran Gigi Tahap 2 di Universitas Brawijaya. Hasil penelitian menunjukkan bahwa penggunaan BIM Revit memberikan manfaat signifikan dalam perhitungan volume material. Kemampuan BIM Revit untuk secara otomatis menghitung volume berdasarkan model 3D meminimalkan potensi kesalahan manusia dan meningkatkan akurasi perhitungan. Dalam konteks Gedung Fakultas Kedokteran Gigi Tahap II Universitas Brawijaya, penerapan BIM Revit secara efektif menghasilkan penghematan waktu dan biaya. Penelitian ini memberikan kontribusi pada pemahaman lebih lanjut tentang manfaat BIM dalam tahap pelaksanaan konstruksi, khususnya dalam perhitungan volume, dengan implikasi positif terhadap efisiensi proyek dan keberlanjutan pembangunan. Temuan ini diharapkan dapat mendorong lebih lanjut adopsi BIM dalam industri konstruksi.
Pengembangan Lembar Kerja Peserta Didik Elektronik (E-LKPD) Berbasis Project Based Learning (Pjbl) pada Materi Invertebrata untuk Melatihkan Kemampuan Berpikir Kreatif Peserta Didik SMA Ayuningtyas, Paramita; Ambarwati, Reni
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 15 No. 1 (2026)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v15n1.p56-68

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Keterampilan berpikir kreatif adalah salah satu tuntutan pada abad ke-21. Pembelajaran berbasis teknologi dianggap sebagai salah satu solusi yang efektif. Namun pada kenyataannya keterampilan berpikir kreatif masih belum optimal dipraktikkan dalam pembelajaran di sekolah. Oleh karena itu, penelitian ini penting dilakukan untuk mengembangkan perangkat pembelajaran yang mampu melatih keterampilan berpikir kreatif secara efektif. Model pembelajaran PjBL dapat membantu melatihkan berpikir kreatif melalui proyek nyata. Tujuan penelitian ini yaitu menghasilkan E-LKPD berbasis PjBL pada materi invertebrata untuk melatih kemampuan berpikir kreatif peserta didik SMA yang valid, praktis, dan efektif. Pengembangan E-LKPD menggunakan model ADDIE ( analisis, desain, pengembangan, implementasi, dan evaluasi ). Uji coba terbatas dilaksanakan pada 33 peserta didik X-3 SMA Negeri 3 Surabaya. Validitas E-LKPD dinilai dari validator yaitu dosen ahli pendidikan dan dosen ahli materi. Kepraktisan diukur dari keterlaksanaan pembelajaran, respon guru dan respon peserta didik. Keefektifan E-LKPD diukur dari indikator ketercapaian berpikir kreatif. Penelitian ini berhasil E-LKPD Berbasis PjBL pada materi invertebrata untuk melatih kemampuan berpikir kreatif peserta didik untuk peserta didik dan pegangan untuk guru. Hasil penelitian menunjukkan validitas E-LKPD memperoleh persentase 98% kategori sangat valid. Kepraktisan E-LKPD dari observasi keterlaksanaan memperoleh persentase 98% kategori sangat praktis, dari hasil respon guru memperoleh persentase 98,8% kategori sangat praktis, dan hasil dari respon peserta didik memperoleh persentase 96% kategori sangat praktis. Hasil penelitian menunjukkan E-LKPD efektif dengan persentase rata-rata ketercapaian indikator berpikir kreatif 90% kategori sangat tinggi. Dapat disimpulkan bahwa E-LKPD berbasis PjBL pada materi invertebrata yang dikembangkan valid, praktis, dan efektif untuk melatih kemampuan berpikir kreatif peserta didik SMA. Kata Kunci: Invertebrata, E-LKPD, Validitas E-LKPD, Kepraktisan E-LKPD, Keefektifan E-LKPD.
Silaturahmi Dan Rapat Koordinasi Pengurus IAAI 2022-2026 Ambarwati, Reni
Jurnal Pengabdian Masyarakat Vol 3 No 4 (2025): Vol 3 No 4 Tahun 2025
Publisher : Sekolah Tinggi Teknik Multimedia Internasional Malang

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Kegiatan pengabdian kepada masyarakat ini dilaksanakan melalui penyelenggaraan Silaturahmi dan Rapat Koordinasi (RAKOR) Ikatan Alumni Arsitektur ITN Malang (IAAI) masa bakti 2022–2026. Kegiatan ini bertujuan untuk membangun kembali semangat kebersamaan, memperkuat komunikasi organisasi, mengevaluasi dan memantapkan arah program kerja, serta meningkatkan sinergi dan kolaborasi antar alumni, dunia profesi, dan dunia industri. IAAI sebagai wadah silaturahmi dan jejaring profesional alumni Arsitektur ITN Malang dari berbagai angkatan memiliki peran strategis dalam mendukung pengembangan institusi dan peningkatan kompetensi lulusan. Melalui forum ini, dilakukan diskusi, evaluasi program, serta perumusan langkah strategis organisasi dalam menghadapi tantangan global industri arsitektur, termasuk perkembangan teknologi dan digitalisasi, isu lingkungan, serta dinamika pasar. Kegiatan ini juga menjadi ruang partisipatif untuk menghimpun masukan dari alumni profesional, Pembina Alumni, serta Ketua Program Studi Arsitektur ITN Malang. Hasil kegiatan menunjukkan adanya penguatan komitmen kepengurusan, peningkatan jejaring profesional yang progresif, serta terbangunnya sinergi kelembagaan antara IAAI, Program Studi Arsitektur ITN Malang, dunia profesi, dan mitra industri.
Analisis Tingkat Kematangan Gonad dan Indeks Kematangan Gonad Kerang Darah (Anadara granosa) di Muara Sungai Banjar Kemuning, Sedati, Sidoarjo Safitri, Eryanti Dwi Hadi; Rahayu, Dwi Anggorowati; Ambarwati, Reni
Bioed : Jurnal Pendidikan Biologi Vol 14, No 1 (2026): Bioed : Jurnal Pendidikan Biologi
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/jpb.v14i1.23435

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Tingginya intensitas penangkapan kerang darah (Anadara granosa) di Muara Sungai Banjar Kemuning berpotensi mengganggu keberlanjutan populasi apabila tidak didukung data biologi yang memadai. Penelitian ini bertujuan untuk menganalisis tingkat kematangan gonad (TKG), indeks kematangan gonad (IKG), serta keterkaitannya dengan kondisi lingkungan perairan. Penelitian dilakukan secara deskriptif kuantitatif dengan pengambilan sampel pada tiga stasiun berdasarkan intensitas penangkapan. Parameter yang diamati meliputi TKG, IKG, serta kualitas air (suhu, salinitas, pH, dan oksigen terlarut). Hasil penelitian menunjukkan bahwa sebagian besar individu berada pada TKG II (22 dari 46 individu), yang menunjukkan fase perkembangan gonad awal hingga pertengahan. Nilai IKG meningkat seiring bertambahnya TKG, dengan kisaran 2,5–7,0% pada jantan dan 2,0–6,0% pada betina. Parameter kualitas air berada dalam kisaran optimal (suhu 29,8–30,9°C; salinitas 33–34 ppt; pH 8,0–8,3; DO 6,58–6,97 mg/L) yang mendukung perkembangan gonad. Namun, rendahnya proporsi individu pada TKG IV (4 individu) menunjukkan bahwa tekanan penangkapan diduga menjadi faktor utama yang memengaruhi belum optimalnya kondisi reproduksi populasi. Kata Kunci: Anadara granosa, tingkat kematangan gonad, indeks kematangan gonad, kualitas air
Gastropods Diversity in the Mangrove Ecosystem of Kalanganyar River, Kalianyar Village, Bangil, Pasuruan Kautsar, Ghinahilmi Najalin; Ambarwati, Reni; Rahayu, Dwi Anggorowati
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p071-078

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Mangrove ecosystems are very important habitats for various organisms, including gastropods, which function as primary decomposers in the ecological cycle. This study aims to identify the diversity of gastropod species in the mangrove area along the Kalanganyar River, Kalianyar Village, Bangil, Pasuruan. Samples were collected randomly around mangrove roots, followed by morphological identification and species classification. The results showed that there were five gastropod species from four families, namely Pirenella cingulata, Telescopium telescopium, Sermyla riquetii, Neripteron violaceum, and Ellobium sp. The Shannon-Wiener diversity index (H') of 1.165 indicates a moderate level of diversity, with Pirenella cingulata as the most dominant species. Water quality tests showed that copper (Cu) levels were still below the threshold, but lead (Pb) levels exceeded seawater quality standards. These results indicate that the mangrove ecosystem is fairly stable, although there are environmental pressures that need to be addressed, particularly those related to heavy metal pollution.