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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Jurnal Pendidikan Biologi Undiksha Jurnal Ilmu Dasar BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Biosains Pascasarjana JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Pendidikan dan Sosial Humaniora SIMBIOSIS
Anak Agung Ketut Darmadi
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Mathematics Veterinary

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JENIS DAN MANFAAT FLORA PULAU DERAWAN KABUPATEN BARAU PROVINSI KALIMANTAN TIMUR Ni Nyoman Darsini; I .G. A. Sugi Wahyuni; A.A.K. Darmadi; Ni Nyoman Wirasiti
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on identication of plants species available on Derawan Island, Berau Regency, East Kalimantan has been conducted based on exploration survey around the island between 2002-2005. The plants whicht have observed were then listed on a table, their scientific names wrre recorded, their status (whether being protected or not) were determined, and the benefits or use of the plants were also listed. From this study it can be found 45 species of 30 families. They may bring benefits for the locals as vegetables, source of medicine, providing shady spots, or decorative plants.
Analisis Pengetahuan dan Sikap Masyarakat Desa Penglipuran, Bangli Terkait Upaya Pelestarian dan Konservasi Tumbuhan Upacara Ngaben Berdasarkan Tri Mandala Ida Bagus Made Bramasta Wira bumi; Eniek Kriswiyanti -; Anak Agung Ketut Darmadi
Jurnal Pendidikan Biologi undiksha Vol. 8 No. 2 (2021)
Publisher : Undiksha

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Abstract

Bali is an island that rely on tourism as its main economy sources. Most of Bali’s tourism destination are in the form of Cultural tourism. Traditional ceremony as a part of culture in Bali held a main factor on supporting Bali’s tourism. Traditional ceremonies in Bali is variated started from giving birth, teenage ceremony, weeding and death. One of the most ceremony attract a lot of tourist each year to watch and visiting Bali is Bali’s Ngaben ceremony. Ngaben as one of the most touristic ceremony in Bali requires a lot of items such as plants and animals as it’s offering. To fulfill the need of plants for Ngaben, society need to culture and cultivated the plants in which needed for the ceremony. People of Penglipuran village, Bangli has divided their village into 3 main part called Tri Mandala. This division causing the plants that planted on each mandala or area are variated and diersified.However, there are no record about the knowledge and behaviour of Penglipuran village, Bangli in accordance to conservation and preservation of plant used in Ngaben ceremony. Hence, data about knowledge and behaviour of the conservation and preservation of plant used in Ngaben ceremony in Penglipuran, Bangli is essential to take futures step on the continuity of the ceremony and tourism. Therefore, the aim of this research is to know the knowledge and behaviour of people on Penglipuran village, Bangli in accordance to conservation and preservation of Ngaben ceremonial plant. This research using purposive sampling techinque in which 5 people were choosen to be key informan and 30 components of the society were choose to be given a quitioner. The result of the research found 31 species of plant used in Ngaben were found among 47 species of plants used in Ngaben at Penglipuran, Village Bangli. Peoples knowledge and attitudes regarding conservation and preservation of Ngaben ceremonial plant is considerably excellent with score for ach component on the society for the knowledge parameter as follows; government with score of 41,  village’s leader with score of 45.4, and 45,2 for society. Meanwhile, for behaviour, the score for government is 102, village’s leader is 97 and society is 93.
Composition Of Mangrove Species On Ngurah Rai Mangrove Forest Pemogan Village, Denpasar Selatan District, Denpasar Municipality, Bali Province Anak Agung Ketut Darmadi; I Putu Gde Ardhana
Jurnal ILMU DASAR Vol 11 No 2 (2010)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The research on analysis of composition of mangrove forest vegetation is a fundamental that can be used toutilize mangrove forest. The research was carried out at Ngurah Rai Mangrove forest, Pemogan village,Denpasar Selatan district, Denpasar Municipality, Bali province be using systematic plot sampling method. Theresearch result showed that there are seven kinds of mangrove tree found, such as Soneratia alba (L.) Blanco,Rhizophora apiculata BL., Avicennia alba Blume, Bruguiera gymnorrhiza (L.) Lamk., Xylocarpusmoluccensis (Lam.) M. Roem., Aegiceras corniculatum (L.) Blanco, and Ceriops tagal (Perr.) C.B. Robinson.Number of S. alba (L.) Blanco growth increase from sea to land. The highest important value was found in S.alba (L.) Blanco.
LOTION PROTECTIVE EFFECT USING LEMONGRASS ESSENTIAL OIL (Cymbopogon citratus) AGAINST Aedes aegypti Ainun Sida; Ni Luh Arpiwi; Anak Agung Ketut Darmadi
Jurnal Biosains Pascasarjana Vol. 25 No. 2 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i2.2023.146-155

Abstract

Lemongrass consists of two types that have many benefits. Its use in research or in processing using its stems alone causes lemongrass leaf waste which can be used as an essential oil and used as an anti-mosquito lotion to reduce the use of chemicals as repellent. The purpose of this study was to determine the yield of lemongrass essential oil, to determine the protective power of each concentration of citronella essential oil in lotion in repelling mosquito bites, and to determine the level of preference for mosquito repellent lotion. The average yield with three repetitions followed by standard deviation (SD) was 0.1% ± 0.0024% b/b. Lotion preparation provides different protection during 6 hours of exposure, namely K- and F1 no protection, F2 for 2 hours, F3 and K+ 4 hours and F4 5 hours. Based on all parameters, the most preferred formulation is F1 and does not give sensitive reactions. Keyword: Aedes aegypti, essential oils, lemongrass, lotions.
Comparison of Zinc ( Zn ) and Cadmium ( Cd ) Levels in Rhizophora Mangrove Species mucronata in Muara Tukad Badung, Bali Ferdinan, David Firman; Darmadi, Anak Agung Ketut; As-syakur, Abd. Rahman; Wijana, Made Sara
Bumi Lestari Journal of Environment Vol 24 No 2 (2024)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843//blje.2024.v24.i02.p04

Abstract

Mangrove forests are an ecosystem that has an important role and function for the environment. Mangrove forests have ecosystems that are very beneficial to humans directly or indirectly. Apart from that, the mangrove ecosystem also has other important functions, namely as a catcher of sediment and as a prevention of erosion and as a soil stabilizer in estuary areas. Data collection was carried out in March using several methods, namely BCF, TF, and Igeo. Sampling was carried out using the Rhizopora type of mangrove mucronata at 3 different stations. The heavy metals tested in this study were zinc ( Zn ) and cadmium ( Cd ). In the highest sediment values for Zn and Cd were 15.516 and 0.532 respectively. In water, the highest levels for Zn and Cd are 0.020 and 0.006 respectively. The highest BCF root values for Zn and Cd are 0.00896 and 0.0609 respectively. The highest leaf BCF values for Zn and Cd are 0.02417 and 0.04487 respectively. The highest TF values for Zn and Cd are 2.68784 and 0.92857 respectively. The Igeo value for Zn is in the unpolluted category and CD is in the slightly polluted category
In vitro assessment of antifungal activity of cinnamon leaves extract against the Colletotrichum sp. causes of anthracnose on tomato Sudirga, Sang Ketut; Darmadi, Anak Agung Ketut; Wijaya, I Made Saka; Yulihastuti, Dwi Ariani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224284-294

Abstract

Tomato is a horticultural plant widely cultivated in Bali. Over the three years, the productivity and quality of this plant have tended to decrease due to pathogen-derived diseases, such as anthracnose caused by Colletotrichum sp. Until recently, control of this pathogenic fungus has relied on chemical-based fungicides, which lead to many long-term complications, including pathogen resistance, environmental pollution, the extinction of non-target microorganisms, and negative impacts on human health. Therefore, alternative methods for plant disease control are urgently needed to combat these pathogen attacks. The use of plant-derived active compounds has been intensively researched worldwide as a more environmentally friendly alternative. The main objective of this research was to investigate the effectiveness of Cinnamomum burmanii acetone extract in inhibiting the growth of Colletotrichum sp., the causative agent of anthracnose in tomatoes, through an in vitro approach. A non-factorial randomized complete design was applied in the experiment. The results showed that the crude extract of cinnamon leaves inhibited the growth of the Colletotrichum sp. with an MIC value of 0.9%, an inhibition zone of 2.55 mm, and an optimal inhibitory concentration of 2%, producing an inhibition zone of 11.10 mm. A GC-MS analysis was conducted to identify the active compounds in the cinnamon leaf extract. Sixteen active compounds were identified, nine of which are known to have antimicrobial activity.
Daya Hambat Ekstrak Kulit Buah Kakao (Theobroma Cacao L.) terhadap Pertumbuhan Bakteri Staphylococcus Aureus Rosenbach dan Escherichia Coli Migula Aulia Nurbaiti; A. A. Ketut Darmadi; Sang Ketut Sudirga
Khatulistiwa: Jurnal Pendidikan dan Sosial Humaniora Vol. 5 No. 4 (2025): Desember : Khatulistiwa: Jurnal Pendidikan dan Sosial Humaniora
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/khatulistiwa.v5i4.7933

Abstract

Cocoa fruit peel (Theobroma cacao L.) is an agricultural waste with potential as an antibacterial agent due to its bioactive compounds. Pathogenic bacterial infections, such as Staphylococcus aureus and Escherichia coli, pose significant health problems, particularly due to the increasing resistance of bacteria to antibiotics. This creates a demand for alternative, safer, and more effective antibiotics. This study aims to evaluate the effectiveness of cocoa fruit peel extract in inhibiting the growth of S. aureus and E. coli, determine the Minimum Inhibitory Concentration (MIC), and identify the bioactive compound groups contained in the extract. The methods used in this study include maceration for the extraction of cocoa fruit peel, the well diffusion method to test the antibacterial activity of the crude extract, and phytochemical testing to identify the bioactive compounds in the extract. The results show that the crude extract of cocoa fruit peel can inhibit the growth of S. aureus with an inhibition zone diameter of 26.75 mm and E. coli with an inhibition zone diameter of 25.75 mm. The MIC test revealed that S. aureus was more sensitive to the extract, with an MIC at a concentration of 0.7%, resulting in an inhibition zone diameter of 5.35 mm, while E. coli showed an MIC at a concentration of 0.8%, with an inhibition zone diameter of 5.36 mm. The most effective concentration was 50%, with inhibition zone diameters of 20.56 mm against S. aureus and 21.06 mm against E. coli. Phytochemical testing showed that the cocoa fruit peel extract contains alkaloid, tannin, saponin, triterpenoid, and flavonoid compounds. Based on the results, cocoa fruit peel extract demonstrates potential as an effective natural antibacterial agent against S. aureus and E. coli.
In vitro antifungal activity of trembesi leaf extract [Samanea saman (Jacq.) Merr.] against Colletotrichum magnum Rossman & Allen, the causal agent of papaya anthracnose Ratnasari, Athalia; Darmadi, Anak Agung Ketut; Inabuy, Fainmarinat selviani; Sudatri, Ni Wayan
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225317-327

Abstract

Papaya fruit production in Bali has decreased over the last three years (2020-2022). One of the contributing factors is infection by Colletotrichum spp., which causes anthracnose. Anthracnose is an airborne disease that infects various plant commodities including papaya. The use of chemical fungicides to control anthracnose can have negative impacts if used long-term. Trembesi [Samanea saman (Jacq.) Merr.] extract has previously been reported to possess antifungal, antiseptic, antibacterial, and antidiabetic properties. This study aimed to determine the effectiveness and category of inhibitory activity, Minimum Inhibitory Concentration (MIC), Lethal Concentration 50% (LC??), and the phytochemical content of trembesi leaves. Methanol extract of trembesi leaves effectively inhibited the growth of Colletotrichum magnum at a concentration of 5%. The experiment used 11 treatments with 4 replications, including control (0% v/v) and extract concentrations of 1% to 10% (v/v), along with a positive control. The corresponding inhibition zone diameters were: 0.00 mm, 12.00 mm, 11.87 mm, 13.62 mm, 16.00 mm, 19.62 mm, 15.75 mm, 16.87 mm, 17.87 mm, 17.87 mm, 18.25 mm, and 20.87 mm, respectively. The minimum concentration of extract showing inhibitory activity (MIC) was 0.3%, while the LC?? value was 0.32%. Phytochemical screening revealed the presence of phenolics, tannins, alkaloids, steroids, terpenoids, flavonoids, and saponins in the trembesi leaf extract.
The THE POTENCY OF CINNAMON (Cinnamomum burmanni Blume) LEAF EXTRACT AS A BIOPRESERVATIVE AGENT FOR FOOD SAFETY OF SATE LILIT: - Darmayasa, Ida Bagus Gede; Ramona, Yan; Darmadi, Anak Agung Ketut; Suanda, I Wayan; Hani, Ni Luh; Shetty, Kalidas
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.2165

Abstract

ARTICLE HIGLIGHTS- Cinnamon leaf extract combats E. coli in traditional Balinese food.- Natural preservative reduces harmful pathogens in sate lilit.- Cinnamon extract offers a safer alternative to synthetic preservatives.- Active compounds in cinnamon leaves inhibit bacterial growth.- Effective biopreservative for enhancing food safety and quality. ABSTRACTEscherichia coli O157:H7 contamination of the meat used in preparing sate lilit, a favorite traditional food in Bali, Indonesia, has been a great concern for both local people and foreigners. Although C. burmanni has been included in its spice ingredients, active compounds that play a significant role in this pathogen have limitedly been elucidated. The main objectives of this research were to investigate the potency of this plant to control contaminants and elucidate possible compounds that prevent such contaminants by applying the disk diffusion method and LCMS analysis, respectively. The results showed that the leaf extract of this plant inhibited the in vitro growth of E. coli O157:7, with minimal inhibitory concentration (MIC) and LC50 values of 4% and 2.59%, respectively. The LCMS analysis chromatogram showed that the plant extract's most active fraction produced nine peaks, representing nine possible active compounds. Among those, three compounds (Azoxystrobin, Stigmatellin Y, and 2-arachidonoyl glycerol) were suspected of contributing to control contamination, especially by E. coli O157:H7.
POTENSI FILTRAT JAMUR Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Aspergillus sp. PADA PAKAN JAGUNG (Zea mays L.) Ni Made Fiona Ranika; Ni Made Gari; Anak Agung Ketut Darmadi
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i01.

Abstract

Corn is a crop that is used as a strategic commodity for Indonesia as it can be used as animal feed. However, corn production is easily decreased due to the presence of Aspergillus sp. fungi which is able to produce aflatoxin that can affect the livestock health problems. Based on this issue, a method of controlling Aspergillus is needed by using Trichoderma sp. fungus filtrate which has antagonistic properties to pathogens. The purpose of this study was to determine the potential of Trichoderma sp. fungus in inhibiting the growth of Aspergillus sp. isolated from corn grain feed, to obtain the concentration of Trichoderma sp. filtrate that is optimal in suppressing the growth of Aspergillus sp. pathogens and to determine the comparison of positive control inhibitory power with Trichoderma sp. filtrate treatment on Aspergillus sp. pathogens. This study used a Complete Randomized Design (RAL) with dual culture methods and diffusion wells with different doses, namely 10% (v / v), 25% (v / v), 40% (v / v), 55% (v / v), and 70% (v / v). The results showed that the fungus and Trichoderma sp. filtrate had a significant effect in inhibiting the growth of Aspergillus sp. (P ≤ 0,05). The percentage of inhibition of Trichoderma sp. in inhibiting the growth of Aspergillus sp. with dual culture method was 68,61 ± 0,83%. Another study of Trichoderma sp. filtrate test on Aspergillus sp. produced the largest clear zone in the 70% treatment with a diameter of 14,81 ± 0,45 mm and the smallest was found in the 10% treatment with a diameter of 9,06 ± 0,55 mm, while in the positive control treatment a diameter of 19,53 ± 0,94 mm was obtained. The filtrate capability is expected to be a solution for related parties in improving the quality of corn grain feed.
Co-Authors . Ramdhoani Abd. Rahman As-syakur Ainun Sida Alprida Yanti Andy Agustina Lande Aulia Nurbaiti Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Ferdinan, David Firman Hani, Ni Luh I Gusti Ayu Sugi Wahyuni I Ketut Endra Primantara I Ketut Ginantra I Komang Alit Adi Sanjaya I Putu Gde Ardhana I Wayan Suanda Ida Bagus Gede Darmayasa Ida Bagus Made Bramasta Wira bumi Ida Bagus Made Bramasta Wirabumi Inabuy, Fainmarinat selviani Inna Narayani Irawati Irawati IRIANI SETYAWATI Kadek Mardika Laila Maghfirotun Ni’mah Martin Joni NI KADEK LOSIANI Ni LUH ARPIWI Ni Luh Arpiwi Ni Made Fiona Ranika Ni Made Gari NI NYOMAN DARSINI Ni Nyoman Wirasiti Ni Wayan Sudatri Putri Permata Ratnasari, Athalia Retno Kawuri Rochmalia Juniarti Putri SANG KETUT SUDIRGA SANG KETUT SUDIRGA Shetty, Kalidas Silviana Febrionita Meiskia Agung Sista Nirmala SURYANINGSIH - Wijana, Made Sara Wijaya, I Made Saka Wira bumi, Ida Bagus Made Bramasta Yan Ramona