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Journal : EKSAKTA: Journal of Sciences and Data Analysis

Optimasi Prosedur Kultur H9C2 Kardiomioblas: Sebuah Pembelajaran untuk Membangun Model Studi In-vitro Kardiovaskular Fadhillah, Muhamad Rizqy; Arozal, Wawaimuli; Wibowo, Heri
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 5, ISSUE 1, April 2024
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol5.iss1.art8

Abstract

Cardiovascular disease become one of the leading factors of death in the world. Thus, research is urgently needed to discover newer drugs or therapeutical agents and biological plausibility. The H9C2 cardiomyoblast originated from embryonic BDIX rat ventricular cells and was previously used in numerous in vitro studies because of its similar nature to cardiomyocytes. However, to our knowledge, there are still limited studies on the basic procedure for culturing H9C2 cardiomyoblast and arranging the best strategy to perform a suitable timeline. Here, we shared our experience in culturing the H9C2 cardiomyoblast, including harvesting and subculturing the cells. We also demonstrated the change of cell confluency, depending on the seeding number, serum concentration, and culture flask through days 1, 3, and 6, to determine their doubling-time population. H9C2 cardiomyoblasts’ doubling time is around 48-54 days with Mean±SD 2.38±0.41. However, seeding density, different culture flasks, and serum concentration have become independent factors in determining specific measures to harvest the cells for further experiments.
Co-Authors Agian Jeffilano Barinda Antarianto, Radiana Dhewayani Arleni Bustami Aryo Tedjo Barinda, Again Jeffilano Cong, Tzeto Han Dewi Sukmawati Dewi, Yulia Ratna Djumhana Atmakusuma, Djumhana Dwita, Nounik Cheri Edhiatmi, Marsetyo Erlina, Linda Erni H Purwaningsih, Erni H Erni Hernawati Purwaningsih, Erni Hernawati Ernie Purwaningsih Eviarini, Prawitasari Eziefule, Oluebube Magnificient Fadhillah, Muhamad Rizqy Fadilah Fadilah, Fadilah Fajarido, Ariski Franciscus D. Suyatna Gusti Khatimah, Nurul Hedi R. Dewoto Heri Wibowo Husna, Fauzul Indah D Dewijanti, Indah D Jusman, Sri Widia Azraki Kapuangan, Christopher Kartika, Kartika Handayani Kenichi Watanabe Khatimah, Nurul Gusti Kuncoro, Daviet Dwi Kurniasari Kurniasari Kusmardi Kusmardi Laurentius, Andrea Lee, Hee Jae Meilei Ma Melva Louisa Musridharta, Eka Nabillah, Deya Adiby Nafrialdi Nafrialdi Novi Silvia Hardiany Nur Azizah Nurwulan Adi Ismaya Pebriani, Mala Primadhani, Suci Widya Punniyakoti T. Veeraveedu Puspita Eka Wuyung Putri, Rizky Clarinta Radiana Dhewayani Radoti Merlin Rani Sauriasari Rani Sauriasari, Rani Raymond R. Tjandrawinata Rejeki, Marliana Sri Rianto Setiabudy Riski Amanda, Clara Ros Sumarny Rosyidah, Citra Safutra, Muhamad Sadam Safutra, Sadam Sagala, Radoti Merlin SEPTELIA INAWATI WANANDI Sesilia Keban Sesilia Keban, Sesilia Shimizu, Ippei Silmi Hanifah Sumiati, Yati Supraja Dwiyono Suyatna, Franciscus D Syarifah Dewi Tri Yuliani, Tri Triana, Novi Ulfa T Wahyuni Ulya, Nurul Vetnizah Juniantito Vivian Soetikno Wahyunia Likhayati Septiana Wikanendra, Gregorius Bhaskara