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Journal : Bionature

Komparasi Enzim Kitinase dari Beauveria bassiana galur Lokal Sulawesi Selatan terhadap Mortalitas Ulat Grayak (Spodoptera litura) Rachmawaty
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

This study aims to determine the enzyme activity of chitinase B. bassiana strain found in South Sulawesi. Knowing the relationship between the activity of chitinase enzyme isolate-isolate B.bassiana strain in South Sulawesi with mortality grayak caterpillar (Spodoptera litura). This descriptive research by linking the enzyme activity of chitinase from Beauveria bassiana strain local South Sulawesi on mortality grayak caterpillar larvae (Spodoptera litura). Based on the research that has been done shows that the activity of the enzyme from South Sulawesi B.bassiana local strains showed differences in each isolate, isolates the origin Enrekang 7.15 units / ml, isolate origin Bantaeng 7.12 units / ml, 6.32 units of isolate origin Pinrang / mi and isolate the origin of Maros 6.2 units / ml. There is a relationship between enzyme activity with mortality rates of larvae of Spodoptera litura. The highest mortality is caused by the isolate origin Enrekang 86%, followed by the isolate origin Bantaeng 83%, 76% Pinrang origin isolates and isolate the origin of Maros 73%.
Pengaruh Bahan Pengikat terhadap Sifat Kimia dan Organoleptik Produk Nugget Tempe Hartati; A. Faridah Arsal; Rachmawaty
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The diversification limitation of exploiting tempe as an alternative nugget raw materials, substitution of meat and fish, and also to optimalize the very abundance resource, so it is necessary to do the right processing to the alternative food resource, which meant to give an additional point. Produce a liked product, also the adjustment of technology, and the cost that reached by the society. The aim of this reseach is to know the kind of glue material which can produce nugget tempe with the best chemical and organoleptic propertics. This research consit of 4 treatment; A1 (tempe nugget from bread powder), A2 (tempe nugget from tapioca), A3 (tempe nugget from sagu), A4 (tempe nugget maizena powder). Water convcentration analyse using oven method. Product was tested by organoleptic test (taste, smell, and tecsture). The result on water concentration analyse, that the smallest water concentration shown by A2 about 161,55% between from treatment A1 abaout 191,75%, A3 abaout 180,02% and A4 182, 45%. The result on organoleptic the teste of A1 product is the most like (75 %) between A2, A3 and A4 treatment. The smell of A2, A3 and A4 is the must like (67 %). And the best tecsture is all treatment.The result of this research is expected to give benefit in supporting the effort saking the alternative nugget glue material by using tempe which owning a high nutrition value. This research it is expected to find a kind if nugget tempe with the good glue, good taste and high nutrition value.
Komparasi Enzim Kitinase dari Beauveria bassiana galur Lokal Sulawesi Selatan terhadap Mortalitas Ulat Grayak (Spodoptera litura) (The Comparation Chitinase Enzyme of Beauveria bassiana Local Strain South Sulawesi to Mortality Spodoptera litura) Rachmawaty
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine the enzyme activity of chitinase B. bassiana strain found in South Sulawesi. Knowing the relationship between the activity of chitinase enzyme isolate-isolate B.bassiana strain in South Sulawesi with mortality grayak caterpillar (Spodoptera litura). This descriptive research by linking the enzyme activity of chitinase from Beauveria bassiana strain local South Sulawesi on mortality grayak caterpillar larvae (Spodoptera litura). Based on the research that has been done shows that the activity of the enzyme from South Sulawesi B.bassiana local strains showed differences in each isolate, isolates the origin Enrekang 7.15 units / ml, isolate origin Bantaeng 7.12 units / ml, 6.32 units of isolate origin Pinrang / mi and isolate the origin of Maros 6.2 units / ml. There is a relationship between enzyme activity with mortality rates of larvae of Spodoptera litura. The highest mortality is caused by the isolate origin Enrekang 86%, followed by the isolate origin Bantaeng 83%, 76% Pinrang origin isolates and isolate the origin of Maros 73%.
Pengaruh Bahan Pengikat terhadap Sifat Kimia dan Organoleptik Produk Nugget Tempe Hartati; A. Faridah Arsal; Rachmawaty
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The diversification limitation of exploiting tempe as an alternative nugget raw materials, substitution of meat and fish, and also to optimalize the very abundance resource, so it is necessary to do the right processing to the alternative food resource, which meant to give an additional point. Produce a liked product, also the adjustment of technology, and the cost that reached by the society. The aim of this reseach is to know the kind of glue material which can produce nugget tempe with the best chemical and organoleptic propertics. This research consit of 4 treatment; A1 (tempe nugget from bread powder), A2 (tempe nugget from tapioca), A3 (tempe nugget from sagu), A4 (tempe nugget maizena powder). Water convcentration analyse using oven method. Product was tested by organoleptic test (taste, smell, and tecsture). The result on water concentration analyse, that the smallest water concentration shown by A2 about 161,55% between from treatment A1 abaout 191,75%, A3 abaout 180,02% and A4 182, 45%. The result on organoleptic the teste of A1 product is the most like (75 %) between A2, A3 and A4 treatment. The smell of A2, A3 and A4 is the must like (67 %). And the best tecsture is all treatment.The result of this research is expected to give benefit in supporting the effort saking the alternative nugget glue material by using tempe which owning a high nutrition value. This research it is expected to find a kind if nugget tempe with the good glue, good taste and high nutrition value.
Karakterisasi Isolat-isolat Beauveria Bassiana Galur Lokal Sulawesi Selatan Rachmawaty
Bionature Vol. 9 No. 2 (2008): 2008
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Beauveria mildew is known as useful natural control agent, due to it’s infection potential agents for controlling insect. The range of its host covers broad range of insects order including Lepidoptera, Coleoptera, Hemiptera and Hymenoptera. In the past, the behavior of B.bassiana was identified based on morphological character, which is have weakness because of environmental effect so the fenotype performance does not manifest the genotype value. Another identification method can be used to complete morphological approach is identification based on physiological behavior (toxin production) and biochemical (protein and isoenzim) where the protein analysis and isoenzim, is order to minimize the influence of environment factor for identification. The research was conducted in Laboratorium Biology UNM, there is 11 isolates B.bassiana used in this research represent location and host. Isolates were analyzed by physiological and isoenzim using electrophoresis. The result of physiological character showed that out of 11 isolates characterized, there are isolates produced beauvericin, interestingly the rest isolates produced unknown on identified metabolites. Based on total band protein pattern and isoenzim showed that there are variation, isolates from the same area or district have more similarity characters than isolates from different area.