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All Journal AL KAUNIYAH Alchemy : Journal of Chemistry Teknosains: Media Informasi Sains dan Teknologi Jurnal Agro Al-Kimia Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Chemistry Indonesian Journal of Chemical Research Walisongo Journal of Chemistry Lontara Abdimas : Jurnal Pengabdian kepada Masyarakat Automotive Experiences Molekul: Jurnal Ilmiah Kimia
Maswati Baharuddin
Universitas Islam Negeri Alauddin Makassar
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mechanical Engineering Nursing Physics Public Health

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Bioelectrochemical Systems (BESs) Technology for The Production of Electrical Energy from Kepok Banana Stem Chadijah, Sitti; Firnanelty, Firnanelty; Baharuddin, Maswati Baharuddin; Sappewali, Sappewali
Indonesian Journal of Chemical Research Vol 11 No 2 (2023): Edition for September 2023
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2023.11-sit

Abstract

Bioelectrochemical Systems (BES) technology is a method for generating electric energy using bacteria as catalysts. The electricity is produced by Microbial Fuel Cells (MFCs), which represent the latest development in biological energy research. This study utilized substrates from banana stems and the bacterium Pseudomonas sp. The research aims to assess the potential of banana stems as a substrate in the MFC system and to determine the effects of adding a combination of an electrolyte solution and an appropriate buffering material to achieve maximum current, potential difference, and power density values. The results showed that the maximum current and potential difference achieved were 1.05 mA and 0.62 V, respectively, with a power density value of 446 mW/m². When combining the KMnO4 electrolyte solution with sodium phosphate buffer, a potential difference of 0.76 V and a current of 1.75 mA were obtained, resulting in a power density value of 911 mW/m². By using the K3[Fe(CN)6] electrolyte solution buffer with potassium phosphate buffer, a current of 1.14 mA was produced, and the power density value reached 406 mW/m².
Antibacterial and Antioxidant Activities of Cutibacterium acnes on Jatropha gossypifolia leaves and Pommetia pinata Bark Zahra, Ummi; Nur, Arfiani; Kurniati, Nunung; Yusril, Yusril; Baharuddin, Maswati
Chimica et Natura Acta Vol 13, No 1 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n1.54007

Abstract

Cutibacterium acnes is a bacterium that can cause inflammation of the skin tissue and lead to acne. Inhibitory activity treatment can be carried out using natural compounds unique to Indonesia as a tropical country, namely Pometia pinnata stem bark and red Jatropha gossypifolia leaves. Pometia pinnata stem bark and red Jatropha gossypifolia leaves are known to have the potential for antibacterial activity. This study aims to combine the two plants as anti-bacterial agents against Cutibacterium acnes and as antioxidants. The method used in this study was maceration, employing ethanol and ethyl acetate solvents for antibacterial and antioxidant activity tests, specifically paper disc diffusion and DPPH assays. The results showed that J. gossypifolia and Pometia pinnata leaf extracts contain flavonoids, steroids, terpenoids, alkaloids, and tannins. The antibacterial activity of a mixture of ethanol extracts from J. gossypifolia and Pometia pinnata leaves against Cutibacterium acnes bacteria was strong, categorized at a concentration of 25%. Additionally, there was no significant difference in the antioxidant capacity between the mixture of ethanol and ethyl acetate extracts. The antioxidant capacity value was approximately 53% AEAC at a concentration of 500 ppm.
Isolation and Characterization of Cellulase Enzyme from Sago Bettle Larvae Febryanti, Amalyah; Baharuddin, Maswati; Abeng, Tendri
Chimica et Natura Acta Vol 13, No 1 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n1.48974

Abstract

Sago beetle larvae are larvae that consume cellulose and convert it into simple compounds with the help of cellulase enzymes. The enzyme is produced by bacteria found in the larvae's intestines. This study aims to characterize the cellulase enzyme from R8W bacteria, which is a cellulolytic bacterium and derived from sago beetle larvae. The characterization in this research included determination of the optimum temperature, the optimum pH, and the optimum substrate concentration of the enzyme. The methods in this study consisted of the production of enzymes; characterization of cellulase enzyme by DNS method; and measurement of cellulase enzyme activity on natural substrates. The results showed that the cellulase enzyme R8W bacterial isolates from beetle larvae were in optimum conditions respectively at a temperature of 50 °C (enzyme activity of 0.070 U/mL), pH 8 (enzyme activity of 0.069 U/mL), substrate concentration of 2% (enzyme activity of 0.063 U/mL); cellulase enzyme activity of R8W bacterial isolates from sago beetle larvae on rice husk cellulose as a natural substrate was 0.103 U/mL. The characteristics of the cellulase enzyme of R8W bacterial isolate from sago beetle larvae had an optimum temperature of 50 °C, an optimum pH of 8, and an optimum substrate concentration of 2%.
Uji Aktivitas Antibakteri Ekstrak Sarang Lebah Hutan (Apis dorsata) terhadap Pertumbuhan Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa Ika Prestianti; Maswati Baharuddin; Sappewali Sappewali
ALCHEMY Jurnal Penelitian Kimia Vol 14, No 2 (2018): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.14.2.13028.314-322

Abstract

Penyakit infeksi akibat bakteri merupakan masalah serius dalam kesehatan. Antibakteri alami yang dapat digunakan untuk menghambat pertumbuhan bakteri yaitu sarang lebah hutan (Apis dorsata) terdiri propolis, kantong madu, kantong telur dan kantong pollen yang mengandung senyawa metabolit sekunder seperti flavonoid, tanin dan asam fenolat. Tujuan dari penelitian ini adalah megetahui aktivitas antibakteri ekstrak sarang lebah hutan dari setiap pelarut yang digunakan dan untuk mengetahui pengaruh konsentrasi ekstrak sarang lebah hutan terhadap pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas aeruginosa. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram dengan lama perendaman 1 jam kemudian diinkubasi selama 3 x 24 jam. Hasil penelitian menunjukkan bahwa aktivitas antibakteri ekstrak metanol sarang lebah memiliki aktivitas tertinggi pada bakteri  E. coli yaitu 3,8 mm pada konsentrasi 8%, ekstrak etil asetat sarang lebah pada bakteri S. aureus yaitu 3,72 mm pada konsentrasi 8% dan ekstrak n-heksan sarang lebah pada bakteri E. coli yaitu 16,1 mm pada konsentrasi 8%. Pengaruh konsentrasi ekstrak yaitu semakin tinggi konsentrasi maka semakin besar pula daya hambat ekstrak terhadap pertumbuhan bakteri S. aureus, E. coli dan P. aeruginosa.Antibacterial Evaluation of Extract Beehive (Apis dorsata) against Growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Bacterial infectious diseases are serious health problems. A natural antibacterial that can be used to inhibit bacterial growth of honeycomb (propolis, honey bag, egg bag and pollen bag) containing secondary metabolite compounds such as flavonoids, tannins and phenolic acids. The purpose of this research is to know the antibacterial activity of honeycomb extract from each solvent used and to know the effect of honeycomb extract concentration against the bacteria growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Antibacterial testing was performed by the method of paper disc diffusion with soaking time 1 hour followed by incubation for 3 x 24 hours. The results show that antibacterial activity of methanol honeycomb extract had the highest activity in E. coli bacteria of 3.8 mm at 8% concentration, followed by those of ethyl acetate honeycomb extract and n-hexan honeycomb extract which have antibacterial activity of 3.72 mm and 16,1 mm at 8% concentration against S. aureus and E. coli, respectively. The effect of extract concentration is the higher the concentration the greater the inhibitory power of extract against the bacteria growth of S. aureus, E. coli and P. aeruginosa.
Effectiveness of HNO3 and NaOH Pretreatment on Lignin Degradation in Areca Leaf Sheath Fibre (Areca catechu L.) for Bioethanol Production Wahyuti Wahyuti; Abdul Karim; Rugaiyah Andi Arfah; Muhammad Zakir; Maming Maming; Maswati Baharuddin
Automotive Experiences Vol. 8 No. 2 (2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ae.13089

Abstract

Areca leaf sheaths are underutilized waste but have a high cellulose content of 72.27%, so they can be utilized for bioethanol production. This research aims to utilize areca leaf waste for bioethanol production through acid (HNO3 5%) and alkaline (NaOH 10%) pretreatment processes, enzyme hydrolysis, and fermentation. Pretreatment using 5% HNO3 and 10% NaOH solutions is carried out because it can break down the lignin bond and release it from cellulose and hemicellulose fibers. The enzymatic hydrolysis process uses cellulase enzymes at 37 °C for 48 hours to produce glucose. Glucose content analysis uses the DNS method and UV-Vis spectrophotometry instruments because it is accurate and can detect glucose in low concentrations. The fermentation process is carried out using Saccharomyces cerevisiae as a fermentation microorganism because it has high efficiency in bioethanol production for a duration of 3, 5, and 7 days. Based on the results of the analysis, pretreatment with HNO3 5% solution reduced the level of lignin in areca leaf sheaths by 2.31%. Meanwhile, pretreatment using a 10% NaOH solution lowered lignin levels to 1.81%. Reduced sugar levels after hydrolysis after pretreatment with HNO3 5% and NaOH 10% were 25.08 mg/mL and 16.37 mg/mL, respectively. The highest concentration of bioethanol in the 5% HNO3 pretreatment was achieved on the 7th day at 16.75%, while that of 10% NaOH on the 5th day was 14.75%. This difference is influenced by the availability of fermentable sugars, where HNO3 substrates take longer to decompose by S. cerevisiae than NaOH substrates. Based on the analysis, the bioethanol contains ethanol, thus the areca leaf sheath fibre feedstock has the potential to assist in the advancement of a sustainable biorefinery process that can reduce dependence on fossil fuels and increase added value.
Co-Authors Abd. Rauf Patong Abdul Karim Abeng, Tendri Ahyar Ahmad Ahyar Ahmad Aisyah Aisyah Al Maratun Sholihati Amalyah Febryanti Amalyah Febryanti Amalyah Febryanti Andi Nur Fitriani Abubakar Arfah, Rugaiyah Arfiani Nur Asriani Ilyas Febryanti, Amalyah Firnanelty, Firnanelty Fitria Azis Fitria Azis Fitriana Fitriana Fitriyani, Jeni Hasnah Natsir Heri Heriyono Ika Prestianti Ilmi Wahyuni Irma Rahayu Ismawanti Ismawanti Jeni Fitriyani Karisma Karisma Karisma, Karisma Kurniati, Nunung Mahmudah, Rafiah Maming Maming Mirna Mirna Muhammad Zakir Musyarrifah Musyarrifah Nunung Kurniati Nur Alfina Nur Asmi Nurdia Asdar Nursiah La Nafie Nurul Fadillah Nurul Khaerah Nurul Magfira Patunrengi, Iswadi Ibrahim Rina Dwismar Riskawati Riskawati Riskawati, Riskawati Rugaiyah Andi Arfah Rustiah, Wa Ode Santi Santi Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali, Sappewali Seniwati Dali Sitti Chadijah Sitti Chadijah Sjamsiah, Sjamsiah St Chadijah St. Chadijah Sufriyana Ali Syamsidar HS Ummi Zahra, Ummi Uto, Sahriani Wahyu Rizandi Wahyuti Wahyuti Wulan Wahyuningsih Yunita, Vivi Alfi Yusril Yusril