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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Journal of Applied Veterinary Science and Technology Buletin Veteriner Udayana Advances in Tropical Biodiversity and Environmental Sciences
I Wayan Bebas
Laboratotium Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Effect of Palmyra Fruit Water–Egg Yolk Diluent with Moringa Leaf Extract on Boar Sperm Membrane Integrity and Motility Gorda, I Wayan; Toli, I Putu Gde Pradnya Nolan; Bebas, Wayan
Advances in Tropical Biodiversity and Environmental Sciences Vol. 10 No. 1 (2026): February 2026 (in progress)
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2026.v010.i01.p02

Abstract

This study aimed to evaluate the effect of palmyra fruit water–egg yolk diluents supplemented with moringa (Moringa oleifera L.) leaf extract on the intact plasma membrane, intact acrosomal membrane, and motility of boar spermatozoa during liquid storage. A completely randomized design was used, with seven diluent formulations varying in concentrations of egg yolk and moringa leaf extract. Each treatment was replicated four times and stored at 15-18°C for up to 72 hours. Semen quality was evaluated at 0, 24, 48, and 72 hours of storage. Data were analyzed using analysis of variance followed by Duncan’s multiple range test. Both diluent formulation and storage duration significantly influenced all semen quality parameters (p < 0.05). The diluent containing palmyra fruit water supplemented with 20% egg yolk and 15% moringa leaf extract produced the highest membrane integrity, motility, and viability with the lowest abnormality rate. The semen maintained acceptable quality for artificial insemination for up to 48 hours of storage. The superior performance of this formulation is biologically associated with the synergistic interaction between carbohydrate-derived energy from palmyra fruit water, phospholipid-mediated membrane stabilization from egg yolk, and antioxidant protection from moringa leaf extract against oxidative stress.
QUALITY OF BERKSHIRE BOAR SEMEN DILUTED WITH A COMBINATION OF BELTSVILLE THAWING SOLUTION AND MELATONIN AT DIFFERENT STORAGE TIMES Pradnyani, Gusti Ayu Putu Indira; Bebas, Wayan; Pemayun, Tjok Gde Oka; Trilaksana, I Gusti Ngurah Bagus; Laksmi, Desak Nyoman Dewi Indira; Susari, Ni Nyoman Werdi
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p29

Abstract

Berkshire pigs are a popular choice for small and large scale pig farming enterprises worldwide, including in Bali. Berkshire pigs are well known for their superior meat quality and relatively fast growth rate. This study aimed to determine the effect of storage duration on the quality of Berkshire boar spermatozoa diluted with Beltsville Thawing Solution (BTS®) supplemented with melatonin. Semen from Berkshire boars was diluted using BTS® supplemented with melatonin at a concentration of 1.0 mM and stored at a temperature of 15 –20°C. Semen quality was evaluated at different storage intervals: (P1) 48 hours, (P2) 60 hours, and (P3) 72 hours. The observed semen quality parameters included sperm motility, sperm abnormalities, sperm viability, and plasma membrane integrity of spermatozoa, which were examined using smear preparations observed under a microscope. In addition, sperm malondialdehyde (MDA) levels as an indicator of oxidative stress were measured using the Porcine MDA ELISA Kit. Duncan’s multiple range test analysis showed that the storage duration of Berkshire boar semen diluted with BTS® supplemented with melatonin was able to maintain sperm motility, abnormality, viability, plasma membrane integrity, and malondialdehyde levels up to 72 hours of storage, with sperm motility of 40.66%, sperm viability of 53.00%, and sperm abnormalities of 6.33%. These values met the minimum standards set by the Indonesian National Standard (SNI 8034:2023) for liquid boar semen. These results indicate that melatonin acts as an antioxidant capable of neutralizing free radicals, thereby inhibiting the occurrence of oxidative stress in spermatozoa cells.
Co-Authors Aerawata, I Wayan Gede Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Annisya Syarifuddin Apriliana, Kadek Soma Apriliani, Karolina Artha Guntur Wahana Brotcorne, Fany Budi, Josephine Aurora David Zulkarnain, David Desak Nyoman Dewi Indira Laksmi DEVI INDRAWATI DND Indira Laksmi Drystiana Yessi Ayu Lesmono Dyonesia Dimitri Maya Mayesta EVA AGUSTINA SIAHAAN Geovany Larastiyani Buyona Haq, Nurul Ikhsanul Hendiyani, Melia Heros, Made Novi Lerianti I Gede Soma I Gusti Agung Arta Putra I Gusti Ngurah Agung Dolly Octa I Gusti Ngurah Bagus Tri Laksana, I Gusti Ngurah Bagus I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Kade Mahardika I Ketut Anom Dada I Ketut Puja I Ketut Sumadi I Made Agus Suryanatha I Made Damriyasa I Made Hermadi Putra I Made Yoga Windu Pradana i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Wayan Gorda I Wayan Piraksa I Wayan Wirata Ika Yuni Astutik Jian Rindawidya Pambudi, Jian Rindawidya juliantari, Ni Komang Ade Komang Gita Permana Kusuma, Putu Wijaya LUH PUTU DHATU HANNY ADNANI MADE KOTA BUDIASA Ni Komang Asni Ni Made Dwi Permata Sari Ni Nyoman Werdi Susari NUR ANIS SAFITRI Pandia, Yoga Mahendra Pradnyani, Gusti Ayu Putu Indira Priharyanthi, Luh Komang Ayu Puteri Putri, Priscilla Mariani Sariyono Rahel Situmorang Ribka Natasia Abel Rinna Noviyati Ndun Sandra, Arista Novi Sewoyo, Palagan Senopati Tani Bina, Elyas Herybertus Teja, Dewa Ngakan Gede Surya Tjok Gde Oka Pemayun Tjok Gede Oka Pemayun Tjok Gede Oka Pemayun, Tjok Gede Oka Toli, I Putu Gde Pradnya Nolan Wahyu Kusuma Atmaja Widiastuti, Wayan Arni Widodo Cipto Subagyo