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All Journal Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Bioplante Microbiology Indonesia Jurnal Keteknikan Pertanian Jurnal Hortikultura Jurnal Pengkajian dan Pengembangan Teknologi Pertanian AGRIVITA, Journal of Agricultural Science Widyariset Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Asian Journal of Agriculture Widyariset Jurnal Risalah Kebijakan Pertanian dan Lingkungan Proceedings Series on Physical & Formal Sciences Biodiversitas Journal of Biological Diversity

Widodo Widodo

Department Of Plant Protection, Faculty Of Agriculture, Institut Pertanian Bogor. Jl. Kamper, Campus IPB Dramaga, Bogor 16680, West Java, Indonesia

Author-ID : 2432568

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Social Sciences

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Potensi Cendawan Asal Tanah Perakaran Bambu Sebagai Endofit dan Agen Biokontrol Penyakit Akar Gada Pada Tanaman Brokoli . Asniah; . Widodo; Suryo Wiyono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The potential of fungi from bamboo rhizosphic soils as endophytic and biocontrol agents of clubroot disease (Plasmodiophora brassicae) on Brocoli. Clubroot, caused by Plasmodiophora brassicae is a serious soilborne disease of plants worldwide, capable of severe infection on broccoli, cabbage and cauliflower. Clubroot is the most destructive diseases on cruciferae in Indonesia. The existing control measures, including biological control do not provide satisfactory result. The objective of the study was to explore fungi from bamboo rhizospheric soils as endophytic that can suppress clubroot disease in broccoli. There were two steps in this study: (1) exploration and identification of fungi from bamboo rhizospheric soils. (2) Effectiveness test of the fungi in suppressing clubroot disease. There were four species of fungi as endophytes in brocoli in this study, e.g Aspergillus sp., Mortierella sp., Paecilomyces sp., and Chaetomium globosum. They had colonized broccoli root endophytically and suppressing clubroot disease. Paecilomyces sp. of endophytic fungi can suppress clubroot diseases incidence for 18.75%.

Control of Banana Wilt Disease Caused by Fusarium oxysporum Schlecht f.sp. cubense (E. F. Smith) Using Crab Shell Powder and Chitosan Widodo Widodo; Heri Harti; Suryo Wiyono
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2780

This research was conducted to evaluate the effectiveness of crab shell powder and chitosan to control Fusarium wilt disease on bananas and to analyze the involved control mechanisms. The effectiveness of crab shell powder and chitosan to F. oxysporum f.sp. cubense was examined in a laboratory (in vitro) and greenhouse (in planta). In vitro evaluation showed that chitosan has an antifungal effect while crab shell powder did not. Application of crab shell powder and chitosan suppressed the disease in green house test. The concentrations of crab shell powder and chitosan that most effective to control Fusarium wilt incidences were 0.25% and 0.10% with an efficacy rate of 66.7% and 83.3%, respectively. The highest disease severity reduction was showed by crab shell powder 0.25% and chitosan 0.50% with an efficacy rate of 56.8% and 59.4%, respectively. Suppression of the disease might be due to the fungicidal effect of chitosan and the increase of the total population of bacteria and chitinolytic bacteria in the rhizosphere when banana seedling roots were treated with crab shell powder or chitosan. Experiment results using the split roots technique exhibited the role of crab shell powder and chitosan potentially to induce the resistance of banana to Fusarium wilt.

Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR Fetrina Oktavia; Kuswanhadi Kuswanhadi; Dini Dinarty; Widodo Widodo; Sudarsono Sudarsono
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.881

The accession of the IRDB 1981 (PN’81) population is a newly introduced and an important rubber tree germplasm while the Wickham clone is a commercial variety one. The objectives of this study were to assess the genetic diversity and the population structure of PN’81 populations and the Wickham clones using 15 EST-SSR loci. Results of the analysis showed that the evaluated SSR primers yielded polymorphic markers. The gSSR 268 primer pairs yielded the most informative markers while HBE 280 primers generated the lowest ones. Results of the genetic diversity analysis supported that the PN’81 population belonged to a single large natural population of rubber trees while the Wickham clones belonged to a different group than that of PN’81. The population structure analysis of the rubber accessions was also in agreement with the results of the genetic diversity analysis. The experiment also indicated that PN’81 populations would be useful for future rubber breeding in Indonesia, especially as the sources of parent clones for rubber tree hybridization programs and rubber tree genetic resource conservation.

COMBINING ABILITY AND HETEROSIS OF CHILLI (Capsicum annuum) RESISTANCE TO ANTHRACNOSE Yulia Irawati; Sriani Sujiprihati; Widodo -
Widyariset Vol 15, No 3 (2012): Widyariset
Publisher : Pusbindiklat - LIPI

Anthracnose (Colletotrichum acutatum) is considered as the major disease in chilli. Resistant variety isimportant for controlling infection of disease, since fungicide application and other cultural controlled methodswere not effective. The aim of this research was to estimate combining ability and heterosis of chilli resistanceto anthracnose. Set population of half diallel crosses which involved 6 parents and 15 crosses with C. acutatumisolate BKT 04 were used in this experiment. Mature green fruit were inoculated using a microinjection method.Disease resistant percentage and lesion diameter were used for estimating of combining ability. Disease incidenceand lesion diameter were used for estimating heterosis. General combining ability of IPB C15 was high for diseaseresistance percentage and lesion diameter. Specific combining ability of IPB C15 x IPB C9 was high for diseaseresistance percentage and specific combining ability of IPB C15 x IPB C10 and IPB C4 x IPB C2 were high forlesion diameter. Negative heterosis was expected in disease resistance traits; IPB C15 x IPB C9 had negativeheterosis for disease incidence and IPB C10 x IPB C9 had negative heterosis for lesion diameter.

Kemampuan Kolonisasi Cendawan Endofit dan Peningkatan Pertumbuhan Bibit Cabai Evan Purnama Ramdan; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Proceedings Series on Physical & Formal Sciences Vol. 2 (2021): Prosiding Seminar Nasional Fakultas Pertanian dan Perikanan
Publisher : UM Purwokerto Press

Endophytic fungi are fungi that live in healthy plant tissues without causing disease symptoms. Endophytic fungi in chili have been tested as both biocontrol agents and growth promoters, but their colonization has not been reported. Therefore, this study aimed to determine the level of colonization of endophytic fungi and its effect on the growth of chili seedlings. A total of 8 endophytic fungi were prepared at a density of 2.8 × 106 CFU mL-1. Then the endophytic fungus was inoculated 2 times, first by soaking the seeds, and secondly by watering the endophytic fungus suspension on chili seedlings aged 3 weeks after sowing. Endophytic fungi were re-isolated on chili seedlings that were 4 weeks old after sowing on the roots and stems to determine their colonization ability. Chili seeds were then maintained for up to 4 weeks after transplanting to observe their growth. The results showed that the endophytic fungal colonization ranged from 26-60% on the chili root, while at the base of the stem it was 20-40% with a different pattern of colonization distribution. In addition, endophytic fungus colonization was also able to increase the shoot height and root length of chili seedlings.

Characteristics of Virus Symptoms in Chili Plants (Capsicum frutescens) Based on RGB Image Analysis Asmar Hasan; Widodo Widodo; Kikin Hamzah Mutaqin; Muhammad Taufik; Sri Hendrastuti Hidayat
AGRIVITA, Journal of Agricultural Science Vol 44, No 3 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.3731

Virus infection in chili plants may cause various symptoms. The complexity of the symptoms and human vision ability often become limiting factors during disease investigations. Digital image analysis is expected to become a method to assist in comprehensively describing the symptoms of plant viruses. A disease survey was conducted on cayenne pepper fields in Southeast Sulawesi Province to observe symptoms of virus infection virtually and to record the symptomatic plant using an RGB camera. The split-channel method is used to process images, followed by multidimensional scaling statistical analysis. Later on, viruses associated with plants were detected serologically. Single or mixed infection of Tobacco mosaic virus, Cucumber mosaic virus, Chili veinal mottle virus, and Pepper mottle virus was confirmed by plant leaves showing yellow-mosaic and mottle symptoms. The digital image analysis method could show variations in the characteristics of symptoms based on digital numbers in that cannot be recognized based on the observation of visual symptoms. A new approach to study the interactions between plant infecting viruses and their effects based on image analysis has also been developed during this research. This method needs to be further validated through testing under controlled conditions, such as inoculating plants with a predetermined type of virus.

The Relationship Between Soil Fertility and Basal Stem Rot Disease in Oil Palm Plantations Evan Purnama Ramdan; Arief Hartono; Giyanto Giyanto; Sri Hendrastuti Hidayat; Widodo Widodo
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.384

In oil palm, Ganoderma boninense causes stem rot disease, which is often difficult to control, and soil fertility status is related to the ecology of G. boninense as a soil-borne pathogen. Therefore, this study aims to evaluate the relationship between soil fertility and stem rot disease as well as appropriate management methods to control the disease. This was carried out at the Nusantara Plantation Company's 7, Unit Kiwah Rejosari-Pematang from June 2021 to January 2022. The determination of observation blocks was carried out selectively using three blocks of land attacked by Ganoderma boninense with the same criteria for the year of planting and the same soil type. Each block consists of five plots. Each plot consisted of five sub-plots, consisting of 3 oil palms for disease severity assessment and soil sampling. The soil for each subplot was composed of 15 samples, which were analyzed for physical and chemical properties of the soil. Determination of fertility status based on the soil research manual published by the Indonesian Bogor Soil Research Center with parameters from the analysis results. The limiting factor for fertility is the cation exchange capacity of the soil, which ranges from 10.07 meq/100 g to 17.68 meq/100 g, and the C-organic content, which ranges from 0.40 to 1.15%. According to chi-square analysis, this fertility-limiting factor is related to disease severity. Therefore, management needs to be done by adding organic matter to the soil, practicing organic or inorganic fertilization, and following the principles of cultivating healthy oil palm plants.

Pengendalian Hama Terpadu pada Peremajaan Sawit Rakyat di Kecamatan Pangkalan Kuras, Riau Siti Rizkah Sagala; Hermanu Triwidodo; Widodo Widodo
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 3 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.3.500

The rapid development of oil palm replanting areas will lead to ecological problems such as plant disturbing organisms. Pest and disease attacks are obstacles farmers face when developing their farms, especially in smallholder oil palm plantations. This study aims to examine the components of cultivation techniques, environmental factors, and human resources that influence the application of IPM on smallholder oil palm replanting land in Pangkalan Kuras District, Pelalawan Regency, Riau Province. The methods used in this study included the purposive determination of 60 respondents, data collection through interviews with farmers, field surveys, and data analysis. The results showed that socio-economic aspects influenced farmers' actions in implementing IPM. Farmer knowledge about pests and diseases of oil palm plants was 36%. Farmers have applied several pest and disease control techniques in oil palm cultivation on replanting land. However, most farmers (60%) are still unfamiliar with the term IPM, need to learn the role of natural enemies in pest control in the field, and lack regular monitoring. Keywords: disease, IPM, palm oil, pest, replanting

Volatile organic compounds from Bacillus subtilis BE6 inhibit and lyse mycelial growth of Sclerotium rolfsii SITI HARDIYANTI; WIDI AMARIA; SRI RAHAYUNINGSIH; SUPRIADI SUPRIADI; WARTONO WARTONO; RITA HARNI; ELI KORLINA; WIDODO WIDODO; MOHAMAD ANA SYABANA; RUSBIANTO WIJAYA
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100123

Abstract. Hardiyanti S, Amaria W, Rahayuningsih S, Supriadi, Wartono, Harni R, Korlina E, Widodo, Syabana MA, Wijaya R. 2026. Volatile organic compounds from Bacillus subtilis BE6 inhibit and lyse mycelial growth of Sclerotium rolfsii. Asian J Agric 10 (1): g100123. https://doi.org/10.13057/asianjagric/g100123. Sclerotium rolfsii is a devastating soil-borne pathogen responsible for significant crop losses worldwide, highlighting the need for effective control strategies. Antagonistic bacteria provide an eco-friendly biocontrol alternative for managing such plant diseases. This study evaluated the antifungal efficacy of Volatile Organic Compounds (VOCs) produced by eight bacterial isolates belonging to the genera Bacillus, Serratia, Brucella, and Burkholderia against S. rolfsii under in vitro conditions. Antifungal activity was assessed using a dual-culture assay in divided petri dishes, followed by Scanning Electron Microscopy (SEM) to assess morphological changes, and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS) for chemical profiling. Among the isolates, indigenous Bacillus subtilis BE6 exhibited the strongest inhibitory effect on S. rolfsii isolate Sc.lb, achieving a growth inhibition rate of 57.2±10.5% compared to the control. Other notable isolates included Bacillus amyloliquefaciens P7 (43.5±7.3%) and Serratia surfactantfaciens S108 (42.7±10.9%). Observation through SEM demonstrated that VOCs from B. subtilis BE6 induce severe hyphal abnormalities, such as shrinkage, wrinkling, and lysis. GC-MS analysis identified 27 VOCs emitted by B. subtilis BE6, with the dominant compounds being cis-2,3-epoxybutane (27.34%), methyl (Z)-N-hydroxybenzenecarboximidate (17.19%), 5-methyl-2-hexanone (14.10%), hexanal (12.46%), and 2,5-dimethylpyrazine (2.89%). These compounds belong to the aldehyde, epoxide, oxime, ketone, and pyrazine groups, all of which are associated with antimicrobial properties. Overall, the results demonstrate that VOCs produced by B. subtilis BE6 effectively inhibit and disrupt the mycelial growth of S. rolfsii, highlighting their potential as eco-friendly biofumigant agents for the managing of soil-borne plant diseases. Further studies are required to validate their efficacy under in vivo and field conditions, as well as to elucidate the individual and synergistic roles of key VOCs.

Variability of agronomic characters related to resistance to stem canker (Phytophthora palmivora) on duku (Lansium domesticum) along Batanghari River, Sumatra, Indonesia ISLAH HAYATI; SURYO WIYONO; WIDODO WIDODO; SOBIR SOBIR
Biodiversitas Journal of Biological Diversity Vol. 20 No. 4 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200426

Abstract. Hayati I, Wiyono S, Widodo, Sobir. 2019. Variability of agronomic characters related to resistance to stem canker (Phytophthora palmivora) on duku (Lansium domesticum) along Batanghari River, Sumatra, Indonesia. Biodiversitas 20: 1127-1132. Duku (Lansium domesticum Correa) is a tropical favorite fruit in South East Asia, including Indonesia. However, currently stem canker disease caused by Phytophthora palmivora Butler (Butler) has been a serious problem on duku plant in Indonesia, especially in Jambi Province. Since duku is vegetatively propagated, selecting resistant mother trees is an effective approach to overcome the disease. This research was aimed to identify mother trees and their progenies that resistant to stem canker pathogen. The research was conducted in two stages. The first one was morphological identification of healthy-looked mother trees grown at four locations: three in Batanghari and one in Muaro Jambi District. The second stage was analyzing resistant seedling progeny of mother trees identified at the first stage. At the second stage, 3 month old seedlings were inoculated with P. palmivora, isolated from rhizosphere of trees grown at the researchs location using Completely Randomized Design. The research found 19 healthy mother trees, and showed similarity index of 0.40 based on eleven identified agronomic characters of mother trees. Cluster analysis revealed 2 identified clusters of mother trees with the identified clusters were independent of plant growth locations. Seedling resistant analysis showed that there were only 5 seedlings expressing resistant traits. Correlation analysis showed that the resistant seedlings were originated from mother trees which tend to have longer leaves, more fruits per branch, more yields, and bigger canopies. This information of the characters can possibly become future reference to choose resistant plant as sources of mother trees to develop and maintain duku populations along the Batanghari River.   

Co-Authors ALI NURMANSYAH Ali Wafa Ana Feronika Cindra Irawati Andika Septiana Suryaningsih Andriani, Desta Anna Feronika Cindra Irawati Anthoni Sulthan Harahap Asmar Hasan Asniah Asniah Bintoro H.M. Bonjok Istiaji Bonny P.W.S. Busyairi Latiful Ashar Dedek Kusvianti devi ayu komalaningrat Dini Dinarty Dini Dinarty DJOKO PRIJONO DWI SUGIPRIHATINI Efi Toding Tondok ELI KORLINA Eryna Elfasari Rangkuti Evan P. Ramdan Fetrina Oktavia Fetrina Oktavia Firmansyah, Raden Arief Gayuh Rahayu Giyanto Giyanto Hari Priwiratama Heri Harti Hermanu Triwidodo ISLAH HAYATI Jekvy Hendra Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Kudang Boro Seminar Kuswanhadi Kuswanhadi Kuswanhadi Kuswanhadi Lia Marliyanti Lilih Naelun Najah Maggy Tenawidjaja Suhartono Maggy Tenawidjaja Suhartono Manohara D. Maria Ulfa Putri Yoeshinda MOHAMAD ANA SYABANA Mohamad Rahmad Suhartanto Muhamad Syukur Muhammad Taufik nFN Sulastri Ni Putu Winda Mahasari Rahmi Sutrisni Ramdan, Evan Purnama RITA HARNI RUSBIANTO WIJAYA SITI HARDIYANTI Siti Rizkah Sagala SOBIR SOBIR SRI HENDRASTUTI HIDAYAT SRI RAHAYUNINGSIH Sriani Sujiprihati Sudarsono Sudarsono Sudarsono Sudarsono Sudirman Yahya Sulastri Sulastri Supramana SUPRIADI SUPRIADI Suryo Wiyono Syaidatul Rosidah Titiek Siti Yuliani Tri Rapani Febbiyanti Umi Sallamatul Isniah WARTONO WARTONO Weni Wilia WIDI AMARIA Yudi Sastro Yudi Sastro Yulia Irawati

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