Widodo Widodo
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Kamper Kampus IPB Darmaga, Bogor 16680, Indonesia

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Control of Banana Wilt Disease Caused by Fusarium oxysporum Schlecht f.sp. cubense (E. F. Smith) Using Crab Shell Powder and Chitosan Widodo Widodo; Heri Harti; Suryo Wiyono
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2780

Abstract

This research was conducted to evaluate the effectiveness of crab shell powder and chitosan to control Fusarium wilt disease on bananas and to analyze the involved control mechanisms. The effectiveness of crab shell powder and chitosan to F. oxysporum f.sp. cubense was examined in a laboratory (in vitro) and greenhouse (in planta). In vitro evaluation showed that chitosan has an antifungal effect while crab shell powder did not. Application of crab shell powder and chitosan suppressed the disease in green house test. The concentrations of crab shell powder and chitosan that most effective to control Fusarium wilt incidences were 0.25% and 0.10% with an efficacy rate of 66.7% and 83.3%, respectively. The highest disease severity reduction was showed by crab shell powder 0.25% and chitosan 0.50% with an efficacy rate of 56.8% and 59.4%, respectively. Suppression of the disease might be due to the fungicidal effect of chitosan and the increase of the total population of bacteria and chitinolytic bacteria in the rhizosphere when banana seedling roots were treated with crab shell powder or chitosan. Experiment results using the split roots technique exhibited the role of crab shell powder and chitosan potentially to induce the resistance of banana to Fusarium wilt.
Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR Fetrina Oktavia; Kuswanhadi Kuswanhadi; Dini Dinarty; Widodo Widodo; Sudarsono Sudarsono
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.881

Abstract

The accession of the IRDB 1981 (PN’81) population is a newly introduced and an important rubber tree germplasm while the Wickham clone is a commercial variety one. The objectives of this study were to assess the genetic diversity and the population structure of PN’81 populations and the Wickham clones using 15 EST-SSR loci. Results of the analysis showed that the evaluated SSR primers yielded polymorphic markers. The gSSR 268 primer pairs yielded the most informative markers while HBE 280 primers generated the lowest ones. Results of the genetic diversity analysis supported that the PN’81 population belonged to a single large natural population of rubber trees while the Wickham clones belonged to a different group than that of PN’81. The population structure analysis of the rubber accessions was also in agreement with the results of the genetic diversity analysis. The experiment also indicated that PN’81 populations would be useful for future rubber breeding in Indonesia, especially as the sources of parent clones for rubber tree hybridization programs and rubber tree genetic resource conservation.
The Relationship Between Soil Fertility and Basal Stem Rot Disease in Oil Palm Plantations Evan Purnama Ramdan; Arief Hartono; Giyanto Giyanto; Sri Hendrastuti Hidayat; Widodo Widodo
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.384

Abstract

In oil palm, Ganoderma boninense causes stem rot disease, which is often difficult to control, and soil fertility status is related to the ecology of G. boninense as a soil-borne pathogen. Therefore, this study aims to evaluate the relationship between soil fertility and stem rot disease as well as appropriate management methods to control the disease. This was carried out at the Nusantara Plantation Company's 7, Unit Kiwah Rejosari-Pematang from June 2021 to January 2022. The determination of observation blocks was carried out selectively using three blocks of land attacked by Ganoderma boninense with the same criteria for the year of planting and the same soil type. Each block consists of five plots. Each plot consisted of five sub-plots, consisting of 3 oil palms for disease severity assessment and soil sampling. The soil for each subplot was composed of 15 samples, which were analyzed for physical and chemical properties of the soil. Determination of fertility status based on the soil research manual published by the Indonesian Bogor Soil Research Center with parameters from the analysis results. The limiting factor for fertility is the cation exchange capacity of the soil, which ranges from 10.07 meq/100 g to 17.68 meq/100  g, and the C-organic content, which ranges from 0.40 to 1.15%. According to chi-square analysis, this fertility-limiting factor is related to disease severity. Therefore, management needs to be done by adding organic matter to the soil, practicing organic or inorganic fertilization, and following the principles of cultivating healthy oil palm plants.