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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Biotropika Current Biochemistry Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Greensphere: Journal of Environmental Chemistry Prosiding Seminar Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Anto Budiharjo
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Veterinary

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Potensi Rizobakteri Pembentuk Endospora dari Brokoli (Brassica oleracea var. Italica) sebagai Agen Biokontrol Ralstonia solanacearum serta Biofertilizer Fauzaan, Muhammad Faishal; Wijanarka, Wijanarka; Kusdiyantini, Endang; Budiharjo, Anto; Ferniah, Rejeki Siti
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jis.%v.%i.%Y.210-226

Abstract

Penyakit Layu Bakteri merupakan penyakit tular tanah yang menyerang tanaman hortikultura yang disebabkan oleh bakteri fitopatogen Ralstonia solanacearum. Komunitas bakteri tanah yang menghuni rizosfer tanaman diketahui memiliki potensi yang baik dalam mendukung pertumbuhan maupun melindungi tanaman dari serangan patogen. Bakteri pembentuk endospora dipilih karena memiliki toleransi yang cukup bagus apabila dihadapkan dengan lingkungan yang tidak cocok bagi pertumbuhannya. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi isolat rizobakteri pembentuk endospora potensial sebagai agen biokontrol melawan R. solanacearum dari rizosfer brokoli sehat di lahan pertanian organik desa Kopeng Kabupaten Semarang serta mengetahui potensinya sebagai agen biofertilizer. Isolasi rizobakteri pembentuk endospora menggunakan metode spread plate; purifikasi isolat rizobakteri menggunakan metode streak plate, karakterisasi dengan uji pewarnaan Gram dan uji pewarnaan endospora, uji antibakteri melawan R. solanacearum menggunakan metode Kirby-Bauer; identifikasi molekuler dengan gen 16S rRNA; konstruksi pohon filogenetik dan uji potensi biofertilizer berupa uji produksi IAA menggunakan reagen Salkowski, uji pelarutan fosfat menggunakan media Pikovskaya dan uji fiksasi nitrogen menggunakan media Nitrogen-free Bromothymol-blue. Hasil penelitian diperoleh 18 isolat rizobakteri pembentuk endospora; isolat RB5 memiliki potensi antibakteri dengan daya hambat kategori sedang (6 mm) dan teridentifikasi sebagai Bacillus subtilis strain FP.PT.1.1-CTCRI dengan nilai similaritas sebesar 98,10% serta mampu memroduksi IAA dan melarutkan fosfat tetapi tidak mampu memfiksasi nitrogen secara kualitatif.
Antibacterial Activity of Freshwater Sponge Oncosclera asiatica Against Escherichia coli and Staphylococcus aureus Wulandari, Dyah; Dewantoro, Giwang; Lunggani, Arina Tri; Suprihadi, Agung; Riani, Catur; Setiawan, Edwin; Farikha, Siti Lutfiatul; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.120-129

Abstract

Freshwater sponges are animals from the Porifera phylum that live in freshwater. The sponge used is Oncosclera asiatica was taken from Kali Porong, East Java. Seventeen isolates of bacteria have been obtained from isolation. Antibacterial potential testing was performed by paper disc inhibition assay using Escherichia coli and Staphylococcus aureus as pathogenic bacteria and amoxicillin as a positive control. The antibacterial activity test showed that four isolates have the potential activity. The isolates with the highest inhibition zones were identified using a 16S rRNA.The results of BLAST showed isolate number 2 was Pseudomonas moraviensis with 99.51% similarity. The phylogenetic tree analysis was build using the MEGA X program. The results of the phylogenetic tree analysis showed that P.moraviensis had a bootstrap value of 100% with a  genetic distance value of 0.001. P. moraviensis isolates screened for the presence of Non-Ribosomal Peptide Synthetase (NRPS) gene by A2gamF and A3gamR primers. The amplification result from NRPS gene showed positive meaning that P.moraviensis genome contained NRPS gene.
MOLECULAR CHARACTERIZATION, PHYTOCHEMICALS SCREENING, AND MOLECULAR DOCKING OF CARDAMOM (Wurfbainia compacta), AND SAMBILOTO (Andrographis paniculata) AGAINST COVID-19 Kurniawati, Mufida Budi; Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3005

Abstract

Cardamom and Sambiloto are phytopharmaceutical plants that produce phytochemical compounds that have the potential to be used to increase immunity against COVID-19. because they contain carotenoids, phenols, anthocyanins, saponins, alkaloids and steroids. This research aims to obtain the molecular characteristics of Cardamom and Sambiloto plants from the Gunungpati area, Semarang by ITS primer, testing phenolic phytochemicals, tannins, flavonoids, saponins and alkaloids followed by molecular docking tests with the 6WX4 protein. SARS-CoV-2. Molecular characterization results show that Cardamom and Sambiloto are similar to Wurfbainia compacta MF802556.1 (100%) and Andrographis paniculate LC646073.1 (84.47%). The results of the phytochemical test screening showed that both plants contain flavonoids. Molecular docking tests were carried out with the compounds Quercetin, Avicularin, Naringenin, 5-hydroxy-7,8,2',5' tetramethoxyflavone, and Retinoic Acid. Retinoic Acid as a test ligand has the greatest potential in inhibiting the 6WX4 protein in the SARS-CoV-2 virus with a binding affinity value of -7.28 and RMSD 0.00.
Exploration of Copper and Lead Resistant Bacteria from Sediments of Kali Baru Estuary Purnomo, Eko; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto; Lunggani, Arina Tri; Az-Zahra, Salwa Zubaidah; Wardaja, Bodhicitta; Maimunah, Siti; Dinalhaq, Putri Cahya
Biotropika: Journal of Tropical Biology Vol. 14 No. 1 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2026.014.01.05

Abstract

Heavy metal contamination, particularly by copper (Cu) and lead (Pb), in aquatic ecosystems poses a significant threat to ecological balance, aquatic organisms, microbial diversity and human health. The Kali Baru Estuary in Semarang, Indonesia, has been reported as a heavily polluted area with Cu and Pb concentrations far exceeding environmental safety thresholds, highlighting the urgent need for sustainable remediation strategies. This study aimed to isolate and characterise bacterial strains resistant to Cu and Pb from sediment samples collected from the Kali Baru Estuary in Semarang as potential bioremediation agents. Bacterial isolation was performed using a serial dilution technique up to 10?², in triplicate, spread onto Marine Agar medium, and incubated at 37°C for 24 h. The obtained isolates were purified and characterised based on their macroscopic and microscopic morphological features. Resistance profiling of the isolates was assessed by exposing them to Cu and Pb at concentrations of 100, 200, and 300 mg/L. A total of 32 bacterial isolates were successfully obtained. Among these, two isolates demonstrated resistance to Cu, and 14 isolates exhibited resistance to Pb at the highest concentration tested (300 mg/L). These metal-resistant bacterial isolates exhibit substantial promise for bioremediation applications aimed at remediating Cu and Pb-contaminated aquatic environments.
Co-Authors AB SUSANTO Abhinaya, Danendra Daryl Ade Fajrian Adila Nawan Hasrimi Agung Suprihadi Agung Suprihadi Ahmad Thontowi Anggoro, Naufal Sebastian Aninditia Sabdaningsih Ariani, Nur Frida Arina Tri Lunggani Asgasatya, Syahid Fattah Az-Zahra, Salwa Zubaidah Barri Pratama Budi Raharjo Bunga Fajriani CATUR RIANI Chatarina Umbul Wahyuni Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Deverina Mergypta Dewantoro, Giwang Dian Wahyu Kemalaputri Dinalhaq, Putri Cahya Dudi Hardianto, Dudi Dyah Ayu Riani Dyah Wulandari Edwin Setiawan Eko Purnomo Elfira, Yolanda Elke Gildantia Elvi Yetti Endang Kusdiyantini Endang Kusdiyantini Fahmi, Maya Fitriana Ilul Farikha, Siti Lutfiatul Fauzaan, Muhammad Faishal Fauziah Citra Rahmawati Fitri Arum Sasi, Fitri Arum Fitria, Lutfiah Rahmadini Florentina Kusmiyati HARTAJANIE, LAKSMI Hasrimi, Adila Nawan Heni Wijayanti, Heni Hermanto, Michael Einstein Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Husna, Faza Laili IS HELIANTI Khoirul Huda Kristina Kristina Kurniawati, Mufida Budi Kusumaningrum, Hermin Laila Nur Faizah Lasria Pardede Lindayani, Lindayani Luthfy AN M Masfuroh Maerani Sumarno Mahmudah, Hawari Rosdiana Maria, Atina Maulana, Anand Reyna Mawarni, Risa Arum Maya Fitriana Ilul Fahmi MG Isworo Rukmi Mia Tri Wardani Monalita, Ramadhebi Muhammad Adib Mubarok Muhammad Mulyadi, Muhammad Muhammad Zainuri Norma Sainstika Pangestu Novita Rahmawati, Novita Nur Endah Wahyuningsih Nurhayati Nurhayati Nurlita Abdulgani Octavia Dewi Christiningrum Olivia Nisa Mumtianah Pardede, Lasria Prayogo, Fitra Adi Raden Mohamad Herdian Bhakti Rahayu, Annisa Rizky Rahmah Putri Sunarno Ratna Cempaka Lingga Rejeki Siti Ferniah Risky Panji Nugroho Risma Wiharyanti Safirah, Dearesty Saniha Adini, Saniha Sayono Setyo Nugroho Shabrina, Jauhara Siti Maimunah Siti Nur Jannah Siti Nur Jannah Siu S.S Langden Sonny Abdi Setiyawan, Sonny Abdi Soowanayan, Chumporn Sri Pujiyanto Suetrong, Satinee Sumarno, Maerani Susiana Purwantisari Teuku Tajuddin Thu'ti Alawiyyah Tony Hadibarata, Tony w wijanarka Wahyu Dewi U. Haryanti Wardaja, Bodhicitta Wien Kusharyoto Wijanarka Wijanarka Yason Lukman Sudjito, Yason Lukman Yohanes Chandrawijaya Yopi Yopi Yuliandini Pangestika