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All Journal Journal of Food and Pharmaceutical Science CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Archive of Community Health Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Jurnal Biologi Udayana Journal of Applied Chemical Science Jurnal Farmasi Klinik Indonesia SINTECH (Science and Information Technology) Journal JFIOnline Journal of Health Sciences and Medicine Journal Sport Science, Healt and Tourism of Mandalika (Jontak) COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Innovative: Journal Of Social Science Research Prosiding Workshop dan Seminar Nasional Farmasi (WSNF) Journal of Food and Pharmaceutical Sciences Jurnal Global Ilmiah
Sagun Chandra Yowani
Program Studi Sarjana Farmasi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Identifikasi Mutasi Gen rpob Isolat MDR Mycobacterium tuberculosis di Bali dengan Metode Nested PCR*) Identification Of rpob Gene Mutation of MDR Mycobacterium tuberculosis Isolate in Bali Using Nested PCR Yowani, Sagung Chandra; Yowani, Sagung Chandra; Wirajana, I Nengah
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 2 (2014)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Tuberkulosis merupakan salah satu penyakit infeksi yang menjadi perhatian untuk ditangani pada program Millenium Development Goals (MDGs), terutama oleh meningkatnya kejadian Multi Drug Resistance (MDR_TB). Menurut WHO, resistensi terhadap rifampisin dan isoniazid telah dikategorikan sebagai MDR-TB. Dari beberapa penelitian, ditemukan bahwa hampir 90 % isolat yang resisten rifampisin, juga resisten terhadap isoniazid. Oleh karena itu,resistensi terhadap rifampisin dianggap sebagai marka penentu (surrogate marker) kejadian MDR-TB. Resistensi terhadap rifampisin dimediasi oleh adanya mutasi pada suatu region 81 bp (RRDR : Rifampicin Resistance Determinant Region) dari gen rpoB. Penelitian ini bertujuan utamanya untuk mengeksplorasi mutasi pada gen rpoB dari MDR-TB yang terjadi pada penderita Tuberkulosis di Bali. Isolat MDR-TB yang digunakan pada penelitian ini merupakanj koleksi Laboratorium Mikrobiologi RS Sanglah, Denpasar. Amplifikasi dilakukan dengan metode Nested Polymerase Chain Reaction menggunakan sepasang outer primer dan sepasang inner primer Hasil penelitian menunjukkan selain mutasi pada daerah RRDR, 5 dari 6 isolat yang dikerjakan memiliki titik mutasi yang sama yaitu pada kodon 418, dengan perubahan asam amino dari asam glutamat menjadi asam aspartat.Oleh karenanya, dapat disimpulkan bahwa terdapat missense mutation   Kata Kunci : Nested PCR, MDR-TB, Mutasi gen rpoB ABSTRACT Tuberculosis is one of the  infectious diseases that is important to be treated in Millenium Development Goals (MDGs) programme, mainly due to the increasing of Multidrug resistant tuberculosis (MDR-TB) cases. According to WHO, MDR-TB was defined as tuberculosis caused by strains of Mycobacterium tuberculosis that are resistant to at least isoniazid and rifampicin. Several studies found that nearly 90% of rifampin-resistant isolates, were also resistant to isoniazid. Therefore, resistance to rifampicin is a surrogate marker of MDR. Resistance to rifampicin is mediated by  mutation in a region of 81 bp (RRDR: Rifampicin Resistance Determinant Region) of the rpoB gene. The aim of this research mainly was to explore mutation of MDR-TB’ rpoB gene of tuberculosis patient in Bali. All MDR-TB isolates used for this research were the collection of Clinical Microbiology Laboratory Of Sanglah Hospital, Denpasar. Amplification were conducted by Nested Polymerase Chain Reaction methods using two pair of  inner and outer primers. The result of this study showed that except at RRDR, five of six isolates had one similar mutation at codon 418 from glutamic acid to aspartic acid. Therefore, it could be concluded that there has been a missense mutation in all isolates. Key words: Nested PCR, MDR-TB, r.poB gene mutation
Identifikasi Mutasi Gen rpob Isolat MDR Mycobacterium tuberculosis di Bali dengan Metode Nested PCR*) Identification Of rpob Gene Mutation of MDR Mycobacterium tuberculosis Isolate in Bali Using Nested PCR Yowani, Sagung Chandra; Yowani, Sagung Chandra; Wirajana, I Nengah
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 2 (2014)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Tuberkulosis merupakan salah satu penyakit infeksi yang menjadi perhatian untuk ditangani pada program Millenium Development Goals (MDGs), terutama oleh meningkatnya kejadian Multi Drug Resistance (MDR_TB). Menurut WHO, resistensi terhadap rifampisin dan isoniazid telah dikategorikan sebagai MDR-TB. Dari beberapa penelitian, ditemukan bahwa hampir 90 % isolat yang resisten rifampisin, juga resisten terhadap isoniazid. Oleh karena itu,resistensi terhadap rifampisin dianggap sebagai marka penentu (surrogate marker) kejadian MDR-TB. Resistensi terhadap rifampisin dimediasi oleh adanya mutasi pada suatu region 81 bp (RRDR : Rifampicin Resistance Determinant Region) dari gen rpoB. Penelitian ini bertujuan utamanya untuk mengeksplorasi mutasi pada gen rpoB dari MDR-TB yang terjadi pada penderita Tuberkulosis di Bali. Isolat MDR-TB yang digunakan pada penelitian ini merupakanj koleksi Laboratorium Mikrobiologi RS Sanglah, Denpasar. Amplifikasi dilakukan dengan metode Nested Polymerase Chain Reaction menggunakan sepasang outer primer dan sepasang inner primer Hasil penelitian menunjukkan selain mutasi pada daerah RRDR, 5 dari 6 isolat yang dikerjakan memiliki titik mutasi yang sama yaitu pada kodon 418, dengan perubahan asam amino dari asam glutamat menjadi asam aspartat.Oleh karenanya, dapat disimpulkan bahwa terdapat missense mutation   Kata Kunci : Nested PCR, MDR-TB, Mutasi gen rpoB ABSTRACT Tuberculosis is one of the  infectious diseases that is important to be treated in Millenium Development Goals (MDGs) programme, mainly due to the increasing of Multidrug resistant tuberculosis (MDR-TB) cases. According to WHO, MDR-TB was defined as tuberculosis caused by strains of Mycobacterium tuberculosis that are resistant to at least isoniazid and rifampicin. Several studies found that nearly 90% of rifampin-resistant isolates, were also resistant to isoniazid. Therefore, resistance to rifampicin is a surrogate marker of MDR. Resistance to rifampicin is mediated by  mutation in a region of 81 bp (RRDR: Rifampicin Resistance Determinant Region) of the rpoB gene. The aim of this research mainly was to explore mutation of MDR-TB’ rpoB gene of tuberculosis patient in Bali. All MDR-TB isolates used for this research were the collection of Clinical Microbiology Laboratory Of Sanglah Hospital, Denpasar. Amplification were conducted by Nested Polymerase Chain Reaction methods using two pair of  inner and outer primers. The result of this study showed that except at RRDR, five of six isolates had one similar mutation at codon 418 from glutamic acid to aspartic acid. Therefore, it could be concluded that there has been a missense mutation in all isolates. Key words: Nested PCR, MDR-TB, r.poB gene mutation
Identifikasi Mutasi Gen rpob Isolat MDR Mycobacterium tuberculosis di Bali dengan Metode Nested PCR*) Identification Of rpob Gene Mutation of MDR Mycobacterium tuberculosis Isolate in Bali Using Nested PCR Yowani, Sagung Chandra; Yowani, Sagung Chandra; Wirajana, I Nengah
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v7i2.165

Abstract

ABSTRAK Tuberkulosis merupakan salah satu penyakit infeksi yang menjadi perhatian untuk ditangani pada program Millenium Development Goals (MDGs), terutama oleh meningkatnya kejadian Multi Drug Resistance (MDR_TB). Menurut WHO, resistensi terhadap rifampisin dan isoniazid telah dikategorikan sebagai MDR-TB. Dari beberapa penelitian, ditemukan bahwa hampir 90 % isolat yang resisten rifampisin, juga resisten terhadap isoniazid. Oleh karena itu,resistensi terhadap rifampisin dianggap sebagai marka penentu (surrogate marker) kejadian MDR-TB. Resistensi terhadap rifampisin dimediasi oleh adanya mutasi pada suatu region 81 bp (RRDR : Rifampicin Resistance Determinant Region) dari gen rpoB. Penelitian ini bertujuan utamanya untuk mengeksplorasi mutasi pada gen rpoB dari MDR-TB yang terjadi pada penderita Tuberkulosis di Bali. Isolat MDR-TB yang digunakan pada penelitian ini merupakanj koleksi Laboratorium Mikrobiologi RS Sanglah, Denpasar. Amplifikasi dilakukan dengan metode Nested Polymerase Chain Reaction menggunakan sepasang outer primer dan sepasang inner primer Hasil penelitian menunjukkan selain mutasi pada daerah RRDR, 5 dari 6 isolat yang dikerjakan memiliki titik mutasi yang sama yaitu pada kodon 418, dengan perubahan asam amino dari asam glutamat menjadi asam aspartat.Oleh karenanya, dapat disimpulkan bahwa terdapat missense mutation   Kata Kunci : Nested PCR, MDR-TB, Mutasi gen rpoB ABSTRACT Tuberculosis is one of the  infectious diseases that is important to be treated in Millenium Development Goals (MDGs) programme, mainly due to the increasing of Multidrug resistant tuberculosis (MDR-TB) cases. According to WHO, MDR-TB was defined as tuberculosis caused by strains of Mycobacterium tuberculosis that are resistant to at least isoniazid and rifampicin. Several studies found that nearly 90% of rifampin-resistant isolates, were also resistant to isoniazid. Therefore, resistance to rifampicin is a surrogate marker of MDR. Resistance to rifampicin is mediated by  mutation in a region of 81 bp (RRDR: Rifampicin Resistance Determinant Region) of the rpoB gene. The aim of this research mainly was to explore mutation of MDR-TBâ?? rpoB gene of tuberculosis patient in Bali. All MDR-TB isolates used for this research were the collection of Clinical Microbiology Laboratory Of Sanglah Hospital, Denpasar. Amplification were conducted by Nested Polymerase Chain Reaction methods using two pair of  inner and outer primers. The result of this study showed that except at RRDR, five of six isolates had one similar mutation at codon 418 from glutamic acid to aspartic acid. Therefore, it could be concluded that there has been a missense mutation in all isolates. Key words: Nested PCR, MDR-TB, r.poB gene mutation
Identifikasi Mutasi Gen rpob Isolat MDR Mycobacterium tuberculosis di Bali dengan Metode Nested PCR*) Identification Of rpob Gene Mutation of MDR Mycobacterium tuberculosis Isolate in Bali Using Nested PCR Yowani, Sagung Chandra; Yowani, Sagung Chandra; Wirajana, I Nengah
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v7i2.165

Abstract

ABSTRAK Tuberkulosis merupakan salah satu penyakit infeksi yang menjadi perhatian untuk ditangani pada program Millenium Development Goals (MDGs), terutama oleh meningkatnya kejadian Multi Drug Resistance (MDR_TB). Menurut WHO, resistensi terhadap rifampisin dan isoniazid telah dikategorikan sebagai MDR-TB. Dari beberapa penelitian, ditemukan bahwa hampir 90 % isolat yang resisten rifampisin, juga resisten terhadap isoniazid. Oleh karena itu,resistensi terhadap rifampisin dianggap sebagai marka penentu (surrogate marker) kejadian MDR-TB. Resistensi terhadap rifampisin dimediasi oleh adanya mutasi pada suatu region 81 bp (RRDR : Rifampicin Resistance Determinant Region) dari gen rpoB. Penelitian ini bertujuan utamanya untuk mengeksplorasi mutasi pada gen rpoB dari MDR-TB yang terjadi pada penderita Tuberkulosis di Bali. Isolat MDR-TB yang digunakan pada penelitian ini merupakanj koleksi Laboratorium Mikrobiologi RS Sanglah, Denpasar. Amplifikasi dilakukan dengan metode Nested Polymerase Chain Reaction menggunakan sepasang outer primer dan sepasang inner primer Hasil penelitian menunjukkan selain mutasi pada daerah RRDR, 5 dari 6 isolat yang dikerjakan memiliki titik mutasi yang sama yaitu pada kodon 418, dengan perubahan asam amino dari asam glutamat menjadi asam aspartat.Oleh karenanya, dapat disimpulkan bahwa terdapat missense mutation   Kata Kunci : Nested PCR, MDR-TB, Mutasi gen rpoB ABSTRACT Tuberculosis is one of the  infectious diseases that is important to be treated in Millenium Development Goals (MDGs) programme, mainly due to the increasing of Multidrug resistant tuberculosis (MDR-TB) cases. According to WHO, MDR-TB was defined as tuberculosis caused by strains of Mycobacterium tuberculosis that are resistant to at least isoniazid and rifampicin. Several studies found that nearly 90% of rifampin-resistant isolates, were also resistant to isoniazid. Therefore, resistance to rifampicin is a surrogate marker of MDR. Resistance to rifampicin is mediated by  mutation in a region of 81 bp (RRDR: Rifampicin Resistance Determinant Region) of the rpoB gene. The aim of this research mainly was to explore mutation of MDR-TBâ?? rpoB gene of tuberculosis patient in Bali. All MDR-TB isolates used for this research were the collection of Clinical Microbiology Laboratory Of Sanglah Hospital, Denpasar. Amplification were conducted by Nested Polymerase Chain Reaction methods using two pair of  inner and outer primers. The result of this study showed that except at RRDR, five of six isolates had one similar mutation at codon 418 from glutamic acid to aspartic acid. Therefore, it could be concluded that there has been a missense mutation in all isolates. Key words: Nested PCR, MDR-TB, r.poB gene mutation
Co-Authors A. W. Dwiputri Ade Ari Sundari Ade Ari Sundari Amelia Putri, Ni Luh Dian Saptari Arifani Siswidiasari Asmara A. A. R. Astuti, M.A.P. Ayu Nyoman Chandra Yustiana Ayu Putu Chika Iswari Anjani, Sang Cista Dewi, Ni Putu Fiona Dek Pueteri Dewi Suryani Deniariasih, N.W. Devi, Ni Wayan Antika Sri Devita Kusdianingrum Dewa Ayu Swastini Dewi Andayani Farmawati Dwicandra, N.M.O. Dyah Subadrika Warma Dewi Endang Kumolosasi Gede Bayu Surya Ekayana, I Heni Pujiastuti I Gede Ketut Sajinadiyasa I Gusti A. A. Santhi Rahmaryani I Gusti Ayu Agung Septiari I Ketut Juniarta I Ketut Tunas I Made Dira Swantara I Nengah Wirajana I Nyoman Gede Budiana I Wayan Kasa Ida Ayu Gendari Ida Ayu Ratih Dwi Nugraha Putri Ida Bagus Nyoman Putra Dwija Indra Juana Adikara Jennifer Tamara Jennifer Tamara Kadek Ratna Sari Dewi Kadek Widya Yuli Hartati Ketut Ratnayani Ketut Widyani Astuti Liangky Syane S Luh Vida Sasmitha Luk Ketut Budi Maitriani M. A. Pratiwi Made Dharmesti Wijaya Made Rai Dwitya Wiradiputra Marfu'ah, Nurul Marlia Singgih Wibowo Mirawati N.K.W. Ni Kadek Ari Cipta Pratiwi Ni Kadek Ariani Ni Ketut Sri Anggreni Ni Komang Sasi Ani Ni Made Febrianti Ni Made Yustikarini Ni Nyoman Pradnya Utari Ni Putu Ariantari Ni Putu Intan Satya Dewi Ni Putu Monica Rosdiana Dewi Paramitha Ni Wayan Sukma Pramitha Sari1 Osamu Iitsuka Pradnyaniti, D.G Putri, Ni Kadek Sri Adnyani Alit Putu Ayu Indrayathi Putu Lita Astriani Rasmaya Niruri Ratna Sari Dewi, Kadek Rini Noviyani Sang Ayu Putu Chika Iswari Anjani Saputra, Gusti Nyoman Oka Sari Dewi, Kadek Ratna Sudarma, I Wayan Ari Syamsul Arifin Tasya Pramiswari Tirtawati, Ni Putu Ayu Wijayakusuma, I Gusti Ngurah Lanang Yayanasri Yustiantara, Putu Sanna