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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Floribunda Jurnal Ilmiah Sains Annales Bogorienses
Nampiah Sukarno
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics Veterinary

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Journal : Jurnal Ilmu Pertanian Indonesia

 1 
Karakteristik Mikoriza Arbuskula Tanaman Serai Wangi (Cymbopogon nardus L.) di Lapangan Ternaungi dan Tidak Ternaungi Nampiah Sukarno; Rahayu Laelandi; Ibnu Qayim; Mega Putri Amelya
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 1 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.1.109

Abstract

Arbuscular mycorrhiza (AM) characteristics of citronella grass in the field have not been reported. This research aimed to study the AM characteristics of citronella grass grown in unshaded and shaded fields. The roots of citronella grass were collected from citronella grass plantations in Cianjur, West Java. The root samples were analyzed for AM structures, namely entry points, intercellular hyphae, arbuscules, and vesicles. The results showed that the citronella grass form AM colonization. The quality of root colonization differed between the two cultivation systems. The unshaded citronella grass had higher root colonization compared to shaded citronella grass. In the unshaded citronella grass, the number of arbuscules was 7 per cm of root length, whereas in the shaded citronella grass was 4 per cm of root length. The types of arbuscules observed were arum and intermediate. There were no differences in the number of entry points in the two cultivated systems, which was 3,5 entry points per cm of root length. The numbers of vesicles and internal hyphae in unshaded citronella grass were lower than that of in the shaded citronella grass. In the unshaded citronella grass, the number of vesicles and intracellular hyphae were 1,5 and 8,5 per cm root length, whereas in the shaded citronella grass were 3,5 and 11 per cm root length, respectively. Shading plants grown in the field were bamboo, banana, coffee, tea, and sugar palm. All the shading plants formed AM symbiosis with a colonization value of 7 to 30%. This research indicates that arbuscular mycorrhiza is an important component in the citronella grass cultivation in unshaded and shaded fields. Keywords: Arbuscule, entry point, intercellular hyphae, root colonization, vesicle
Isolasi dan Respons Tumbuh Cendawan Mutualistik Akar pada Beberapa Tanaman Pangan dan Kehutanan Rida Oktorida Khastini; Nampiah Sukarno; Utut Widyastuti Suharsono†; Yasuyuki Hashidoko†
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 1 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.1.85

Abstract

The study aims to isolate and test the effectiveness of mutualistic root symbiont fungi isolates from the roots of rubber plants grown in marginal acidic soil plantations in increasing the growth of food crops and forestry plants. The fungal were isolated by root surface sterilization methods. We obtained 19 fungal isolates consisting of 8 genera, namely Alternaria, Aspergillus, Cladosporium, Curvularia, Fusarium, Penicillium Paecilomyces, Trichoderma, and mycelia sterilia. All isolates were subjected to a pathogenicity test on the Centrosema pubescens plant. Five out of the 19 fungal isolates increased plant growth and showed no disease symptoms, and the Aspergillus section Nigri FKK 3 isolate showed the best response. The isolate was further analyzed to assess the growth response of food crops (rice and corn) and forestry plants (Acacia auriculiformis and Paraserianthes falcataria). The treatments consisted of 3 phosphate (P) concentrations, namely 20%, 50%, and 100% of the recommended field applications. The combination of mutualistic fungal inoculation of Aspergillus section Nigri FKK 3 and 50% P concentration exhibited the highest biomass growth response compared to other treatments. This finding can provide basic information for developing fungal-based fertilizers to increase the productivity of food crops and forestry plants on sub-optimal land. Keywords: food crops, phosphate fertilizer, forestry trees, plant growth improvement, root mutualistic fungi
Isolasi, Identifikasi, dan Produksi Miselia Rhizopus sp. Berkadar Asam Nukleat Rendah untuk Pengembangan Mikoprotein Firmansyah, Riza; Sukarno, Nampiah; Suharsono, Utut Widyastuti; Sukarno, Sukarno; Fadillah, Wendi Nurul
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.179

Abstract

Fungi, including Rhizopus sp., are food sources that commonly contain high nucleic acid levels. Therefore, the nucleic acid content must be reduced to achieve health standard requirements. This study aimed to isolate, identify, and produce Rhizopus sp. mycelium containing low nucleic acid. The Rhizopus spp. were isolated from tempeh collected from 12 different areas in Indonesia. Fungal identification was conducted based on morphological characteristics. The fungal isolates were selected based on mycelial growth and spore production on PDA. Biomass production of mycelium was carried out in potato extract and soybean extract media obtained from 200 g/L and 333.3 g/L, respectively. In each medium, 6 sugar levels were added, namely 0, 2, 3, 4, 5, and 6 g/L. Mycelium nucleic acid content reduction was achieved by heat treatment at 50°C and 60°C for 15 minutes and measured by a spectrophotometer at 260 nm. Fifty-eight isolates that were identified into 3 species were obtained in this experiment: R. oryzae, R. stolonifera, and R. microsporus. R. Microsporus had higher mycelium biomass and lower spore number than the other species. R. Microsporus produced a higher mycelium biomass in the soybean extract medium with 5 g/L additional sugar. The nucleic acid content of the 50°C heat-treated mycelium was 1.82% and 1.73% at 65°C. These values fulfilled the standard of mycelial nucleic acid content permitted in food by the USDA. Keywords: morphology, Rhizopus microspores, spore, soybean extract, tempeh
Co-Authors , Triadiati ADI YULANDI Agustin Wydia Gunawan Alex Hartana ALEX HARTANA Alex Hartana Anastastia Raditya Hidayanti Astri Ariyani Atit Kanti Atit Kanti Cici Rahmawati Cinta Badia Br Ginting, Rohani Dedi Fardiaz Desi Maria Panjaitan Didiek Hadjar Goenadi Dyah Iswantini Edhi Sandra Edi Guhardja Endang Sukara Eris Septiana Fachrian Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadillah, Wendi Nurul Franco, Christopher MM Hamim Hamim HAPPY WIDIASTUTI Hidayanti, Anastastia Raditya I Made Sudiana I Nyoman Pugeg Aryantha Ibnu Qayim Irmanida Batubara Kartini Kramadibrata Kemala S. Nagur L K Darusman Lady Diana Tetelepta, Lady Diana Latifah K Darusman latifah K Darusman Latifah K. Darusman Latifah Kosim Darusman Latifah Kosim Darusman LATIFAH KOSIM DARUSMAN M. Rafi M. Sukarno Mashuri Waite Mashuri Waite Maulani, Nail Izzatul Mega Putri Amelya Michael Michael Mien Ahmad Rifai Mujahidah, Shofia Mujahidah, Shofia Mulya Sari Nadia T. Hendartina Nadiya Dwi Rahayu Novriyandi Hanif Partomuan Simanjuntak Putra, Sukma Triperdana Rahayu Laelandi Rahayu, Nadiya Dwi Rahminiwati, Min Rida Oktorida Khastini Risna Riza Firmansyah Riza Zainuddin Ahmad ROHANI CINTA BADIA GINTING Rohani Cinta Badia Ginting Sally SMITH Sihegiko Kanaya SIHEGIKO KANAYA Sintho Wahyuning Ardie Soekisman Tjitrosoedirdjo Sofi Mursidawati Sofi Mursidawati Sri Listiyowati Subagya, Mahmud Sukarno Sukarno Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulfiah, Siti Syaiful Anwar Utut Widyastuti UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Utut Widyastuti Suharsono† Waite, Mashuri Wei Zhang Wendi Nurul Fadillah Wendi Nurul Fadillah Yanti Novera Yasuyuki Hashidoko† Yuyun Nisaul Khairillah Za’aziza Ridha Julia