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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Penyuluhan Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Kesehatan Lingkungan indonesia JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan Current Biomedicine

Hadri Latif

IPB University

Author-ID : 2480267

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Indirect Enzyme Linked Immunosorbent Assay Sebagai Metode untuk Melacak Bruselosis pada Sapi Perah (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW) Rinaldi Ghurafa; Denny Widaya Lukman; Hadri Latif
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated sensitivity and specificity, as well as analyzed by calculating the kappa value. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated for sensitivity and specificity, as well as analyzed by calculating the Kappa statistical value. The results of the sensitivity and specificity calculation showed that the indirect enzyme linked immunosorbent assay (I-ELISA) test developed a higher sensitivity (100%) compared to RBT test (93.75%) and commercial I-ELISA (93.75%). The developed I-ELISA specificity (74.68%) was still lower than RBT (89.87%), but higher than commercial I-ELISA (70.52%). The calculation of the statistical value of kappa RBT with CFT showed the kappa value 0.7120 which meaned it had a good agreement, commercial I-ELISA with CFT showed kappa value 0.6165 which meaned it had good suitability, whereas I-ELISA developed with CFT showed kappa value 0.4984 which meaned having a moderate agreement.In conclusion, the indirect enzyme linked immunosorbent assay (I-ELISA) which had been developed had low specificity, but the sensitivity was the highest compared to the commercial I-ELISA test and RBT, so this test was appropriate to be used as a screening test, especially in dairy cows movement into brucellosis-free areas or regions.

Phenotypic and Serotypic Characterization of Staphylococcus aureus Strains from Subclinical Mastitis Cattle (KARAKTERISASI SECARA FENOTIPE DAN SEROTIPE STAPHYLOCOCCUS AUREUS YANG BERASAL DARI MASTITIS SUBKLINIK PADA SAPI) Siti Gusti Ningrum; Wyanda Arnafia; Sylvia Oscarina; Retno Damajanti Soejoedono; Hadri Latif; Mohammad Ashraf; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.659 KB)

Staphylococcus aureus is known as a major causative agent of mastitis in dairy cattle. In the presentstudy, 104 isolates of Staphylococcus originated from subclinical mastitis cattle characterized for thephenotypic properties and the presence of Staphylococcal protein A (Spa). Some bacteria were resistancesagainst several antibiotics were also studied, such as erythromycin, streptomycin, tetracycline, cefepime,nitrofurantoin, amikacin, chloramphenicol, and ciprofloxacin. About 78% of the isolated were moderatelysensitive to nitrofurantoin, while 89% were highly resistant to cefepime and ciprofloxacin. Using thevarious mammals’ sera, seven isolates out of 104 revealed the presence of Spa.

Residu Zeranol dalam Daging Sapi yang Diimpor dari Australia dan Selandia Baru Melalui Pelabuhan Tanjung Priok (ZERANOL RESIDUE IN BEEF MEAT IMPORTED FROM AUSTRALIA AND NEW ZEALAND THROUGH THE PORT OF TANJUNG PRIOK) Siti Khadijah; Hadri Latif; Agatha Winny Sanjaya
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.737 KB)

Zeranol is one of the synthetic growth hormone produced from mycotoxin that could affect humanhealth. The objective of this study was to determine zeranol residue in beef meat imported from Australiaand New Zealand using Enzyme-Linked Immunosorbent Assay (ELISA). The results showed that zeranolresidue was detected in 5 of 59 meat samples (8,5 %) of Australia and 1 of 59 meat samples (1,7 %) of NewZealand, with mean concentration of 0,644±0,157 ppb and 0.680±0.00 ppb, respectively. There were nosignificant differences in the concentration of zeranol residue between the meat from both countries(p>0,005). In addition the concentration of zeranol residue was below the National Standardization Agencyof Indonesia Maximum Residue Limit (MRL) which is 2 ppb.

Komposisi Kimiawi dan Fraksinasi Protein Susu Kuda Sumba (THE CHEMICAL COMPOSITION AND PROTEIN FRACTIONATION OF SUMBA MARE’S MILK) Annytha Ina Rohi Detha; Mirnawati Sudarwanto; Hadri Latif; Frans Umbu Datta
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.579 KB)

The aims of this study were to determine both chemical composition and fraction of the proteincompounds of sumba mare’s milk. Determination of the chemical compositions of sumba mare’s milk havedone by analyzing protein content using the Kjeldahl method, fat content using Gerber method, lactosecontent and the total solids content. Identification of antimicrobial compounds of whey proteins in milkusing high performance liquid chromatography (HPLC) method. The results showed that the average ofsumba mare’s milk contained protein, fat, lactose and total solids were; 1.82%, 1.67%, 6.48% and 11.37%respectively. The average value of protein and fat in sumba mare’s milk was decrease significantly at fifthmonth of lactation period. Based on identification of antimicrobial compounds using HPLC method, thereare six main peaks with different polarities and retention times. In conclusion, sumba mare’s milk havea balance composition that can be used as a source of nutritious food and the milk likely also has six mainantimicrobial compounds in its whey protein.

Resistensi Antibiotik Terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat (ANTIBIOTIC RESISTANCE TO ESCHERICHIA. COLI ISOLATED FROM BROILER FARMS IN SUBANG DISTRICT, WEST JAVA PROVINCE) Aji Barbora Niasono; Hadri Latif; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic resistance in avian bacterial pathogens is a common problem in the Indonesia poultry industry. The present study aimed to provide information on the present status of antibiotic resistance in Escherichia coli (E. coli) from a broiler farm environment. The research method uses a cross sectional study with a stratified random sampling pattern. E. coli was collected from litter using boot swab method at the broiler house farms in Subang District. E. coli isolates (n = 74) were obtained from 74 samples of boot swabs. The sample was isolated and identified morphologically and biochemically using the IMVIC test to obtain E. coli isolates. The test was followed by a sensitivity test for nine antibiotics, namely tetracycline, sulfamethoxazole, trimethoprim, ampicillin, nalidixic acid, ciprofloxacin, enrofloxacin, gentamicin and chloramphenicol by using the agar dilution method. The interpretation of the results of this sensitivity test refers to the Clinical and Laboratory Standards Institute CLSI VET01S (2015) and CLSI M100 (2018). Measurement of the level of knowledge is done using a structured questionnaire that has been tested for validity and reliability. E. coli was isolated from 74 samples of boot swab. 93.2% of E. coli samples were found to be MDR with the highest prevalence in four types of antibiotics at 40.5%. The most common resistances observed were against tetracycline 97.3%, sulfamethoxazole 87.8%, trimethoprim 74.3%, ampicillin 68.9%, nalidixic acid 64.8%, ciprofloxacin 45.9%, enrofloxacin 40.5%, gentamicin 28.4% and chloramphenicol 10.8%. The findings of this study revealed the high level of antibiotic resistance in broiler environment is worrisome and have negative implications for human and animal health.

Keberadaan Escherichia coli Extended Spectrum ?-lactamase Resistan Antibiotik di Peternakan Sapi Perah Cijeruk, Bogor Herwin Pisestyani; Denny Widaya Lukman; Hadri Latif; Mirnawati Sudarwanto
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.505

Contamination of Extended spectrum-betha lactamase (ESBL) producing Escherichia coli in milk can cause public health problems. The misuse of antibiotics in dairy has implications for the emergence of multi-resistant bacteria that can infect humans through food (foodborne diseases). The ESBL producing E. coli is resistant to ?-lactam antibiotics (including penicillins and 3rd and 4th derivatives of cephalosporins). This study aimed to analyze the phenotype of antibioticresistant ESBL-producing E. coli from livestock, farmer, dairy cows, milk, and pasteurized milk. The research was conducted on a dairy farm in Cijeruk District, Bogor. The method used was the ESBL EC tricycle for phenotype confirmation consisting of culture on tryptone bile x-glucuronide (TBX) agar and MacConkey agar (MCA) with the addition of the cefotaxime antibiotic 4 µg/mL for the presumption of ESBL-producing E. coli followed by confirmation of ESBL producing E. coli with indol biochemical tests with sulfite indole motility (SIM) and double disk diffusion test (DDST) on Mueller Hinton agar (MHA). The results from this study showed that ESBL producing E. coli was not found in water, effluent, milker hand swabs, teat swabs, and milk from the individual cows. ESBL producing E. coli was identified from fresh milk (2/10) and pasteurized milk (2/10) on the same farm. ESBL-producing E. coli were resistant to eight antibiotics (ampicillin, cefotaxime, ceftazidime, cefpodoxime, cephalothin, streptomycin, azithromicyn, enrofloxacin). Multidrugresistant ESBL-producing E. coli is one of the foodborne diseases that pose a health threat to the community.

Co-Authors . Sumitro . Yudi AGATHA WINNY SANJAYA Agik Suprayogi Agustin Indrawati Ahmad, Hasniah Aji Barbora Niasono Annytha Ina Rohi Detha Asep Yayan Ruhyana B. Sudarwanto, Mirnawati Chaerul Basri Cholilia Abadiatul Masruroh Debby Fadhilah Pazra Dede Sri Wahyuni Denny Lukman Denny W. Lukman Denny Widaya Lukman Diyan Cahyaningsari Dwi Desmiyeni Putri Elsharkawy, Sara Etih Sudarnika Fitri Hidayati Heru Susilo Heru Susilo Herwin Pisestiyani Herwin Pisestyani I wayan Teguh Wibawan Julia Rosmaya Riasari koekoeh santoso Lina Noviyanti Sutardi Loisa Loisa Loisa, Loisa Melani Wahyu Adiningsih Mirnawati B Sudarwanto Mirnawati B Sudarwanto Mirnawati B. Sudarwanto Mirnawati B. Sudarwanto Mirnawati Bachrum Sudarwanto Mirnawati Bachrun Sudarwanto Mirnawati Sudarwanto Mohammad Ashraf Nadhear Nadadyanha Dannar Navasuriya Radha Krisnan Nugraha, Arifin Budiman Okti Nadia Poetri Platika Widiyani Qing Wen, Jyn Soong Rahmat Setya Adji Raihan, Muhammad Ammar Raphaella widiastuti Ratna Normaliska Retno Damajanti Soejoedono Retno Damayanti Soejoedono Rifky Danial Rinaldi Ghurafa Risma Juniarti Silitonga Rizal Eko Kurniawan Saimah Saimah Saimah, Saimah Siti Gusti Ningrum Siti Khadijah Sudarwanto, Mirnawati Bachrum Susanti, Oli Sylvia Oscarina Trioso Purnawarman Umi Cahyaningsih Undari, Fajar Okta Wahyudi, Ading Widiyani, Platika Wyanda Arnafia Yessy Anastasia Yunindika, Thufeil Yusuf Ridwan

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