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All Journal Indonesian Journal of Pharmaceutical Science and Technology Industria: Jurnal Teknologi dan Manajemen Agroindustri Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi Mejuajua Pharmaceutical Reports Jurnal Pengabdian Masyarakat Bhinneka Indonesian Research Journal on Education Media Farmasi Borneo Journal of Pharmacy
Muammar Fawwaz
Laboratory Of Pharmaceutical Chemistry, Faculty Of Pharmacy, Universitas Muslim Indonesia, Makassar
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Journal : Indonesian Journal of Pharmaceutical Science and Technology

 1 
Evaluation of Antioxidant Activity of Vernonia amygdalina Leaves and Its Flavonoid-Phenolic Content Muammar Fawwaz; Mamat Pratama; Musafira Musafira; Indira Wahab; Rina Iriani; Aminah Aminah; Andi Trihadi Kusuma; Muhammad Ikhlas Arsul
Indonesian Journal of Pharmaceutical Science and Technology Vol 10, No 2 (2023)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v10i2.41617

Abstract

Bitter leaf, Vernonia amygdalina (VA), is used in various alternative medicine in several countries,including Indonesia. This study aims to evaluate the antioxidant activity of VA leaves by three differentin vitro methods. The antioxidant activity was evaluated by radical scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidantcapacity (CUPRAC). The antioxidant capacity by FRAP and CUPRAC methods were expressed asmg of quercetin equivalents (QE) per g extract (mgQE/g) and mg of ascorbic acid equivalents (AAE)per g extract (mgAAE/g), respectively. All methods were measured by UV-Visible spectrophotometryat maximum wavelength. The results exhibited that VA leaves ethanolic extract contains flavonoidphenolic.The VA leaves ethanolic extract has an antioxidant activity by scavenging the DPPH methodwith the half inhibition concentration (IC50) was 8,22 μg/mL. The antioxidant capacity of VA leavesethanolic extract by FRAP and CUPRAC methods was 21,03 mgQE/g and 160,64 mgAAE/g extract,respectively. The VA leaves ethanolic extract has a powerful antioxidant potency which might correlateto the flavonoid-phenolic content in the extract.
Antioxidant Activity and Total Carotenoids Level of Litopenaeus vannamei Fawwaz, Muammar; Baits, Muzakkir; Pratama, Mamat; Abidin, Zainal
Indonesian Journal of Pharmaceutical Science and Technology Vol 11, No 3 (2024)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v11i3.41481

Abstract

Udang putih (Litopenaeus vannamei) merupakan salah satu spesies Crustacea yang cangkangnya mengandung senyawa karotenoid. Senyawa ini merupakan antioksidan alami yang berpotensi tinggi. Penelitian ini bertujuan untuk mengetahui karotenoid total pada ekstrak cangkang L. vannamei dan mengevaluasi aktivitas antioksidannya secara in vitro. Ekstrak diperoleh dengan cara maserasi menggunakan n-heksan. Tingkat total karotenoid dalam ekstrak ditentukan setara dengan β-karoten. Spektrofotometer UV-Visible mengukur ekstrak dan larutan standar referensi pada panjang gelombang maksimum β-karoten (462 nm). Dua metode berbeda digunakan dalam menentukan aktivitas antioksidan ekstrak; metode peredaman radikal 1,1-diphenyl-2-picryl-hydrazyl (DPPH) dan metode ferric reducing antioxidant power (FRAP). Hasil penelitian menunjukkan bahwa karotenoid total dalam ekstrak cangkang L. vannamei adalah 5,87 mg/g, setara dengan β-karoten. Konsentrasi hambat setengah (IC50) ekstrak dengan metode DPPH adalah 27,59 µg/mL menunjukkan potensi sedang sebagai antioksidan dibandingkan dengan kuersetin standar sebesar 1,28 µg/mL. Kapasitas antioksidan ekstrak dengan metode FRAP dinyatakan dalam mg setara kuersetin (QE) per g ekstrak (mgQE/g), dan hasilnya menunjukkan potensi penghambatan sebesar 1,65 mgQE/g ekstrak. Dengan demikian, dapat disimpulkan bahwa ekstrak cangkang udang vannamei sangat potensial sebagai sumber karotenoid alami dengan efek antioksidan yang memadai.
Co-Authors A. Muflihunna A. Muflihunna Ahmad Najib Ahmad, Aktsar Roskiana Ainunnisa, Mufti Akbar, Harul Aminah Aminah Aminah Aminah Aminah Aminah Amula, Andi Tenri Andi Trihadi Kusuma Andika, Yusrianti Anggraeni, Bella Ardin, Nisradhiyah Caesarika Arifin, Marina Asni Amin Asriani Suhaenah Astika, Elvina Audia Triani Olii, Audia Triani Ayu Natalisnawati Bambang Sampurno, Bambang Dewi Yuliana Dian Harisman Diana Syam Muliadi Fairuz Dyah Widowati Faradiba Faradiba Faradiba Feralia, Feralia Harti Widiastuti Indira Wahab Lukman La Basy Mamat Pratama Mamat Pratama Mamat Pratama, Mamat Masdiana Tahir, Masdiana Muhammad Ikhlas Arsul Musafira Musafira Muzakkir Baits Muzakkir Baits, Muzakkir Nur Alifah Nur Ramadhana Dewi Safitri, Nur Ramadhana Dewi Nurkamilah, Andi Permatasari, Andi Rahayu, Rita Rahmawati Rahmawati Rahmawati, Rahmawati Rais Razak Rais Razak, Rais Rina Iriani Ririn Ririn Riski Mentari Jahri Saparuddin Latu Seniwati Dali Seniwati Seniwati Seniwati Seniwati, Seniwati Siti Nurdiansyah A Tadjuddin Naid Ummum, Andi Yulianto, Yuni Zakinah Zainal Abidin Zakinah , Yuni