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All Journal Medical Laboratory Technology Journal Meditory : The Journal of Medical Laboratory Jurnal Skala Kesehatan Jurnal Labora Medika Husada Mahakam: Jurnal Kesehatan Jaringan Laboratorium Medis Tropical Health and Medical Research Journal Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Karya Generasi Sehat
Ratih Dewi Dwiyanti
Medical Laboratory Technology Department, Poltekkes Kemenkes Banjarmasin, Indonesia
Religion Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary Other

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Antithrombotic Activity and Hemolysis Pattern of Fibrinolytic Protease-Producing Bacterial Isolates from the Coast of Tanjung Dewa, South Kalimantan Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima; Rizal, Muhammad; Mufid, Muhammad
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.689

Abstract

Cardiovascular disease is a leading cause of death worldwide, with thrombosis playing a key role in the pathological process. Conventional antithrombotic therapy often causes side effects, necessitating alternatives based on biological resources. Marine microorganisms are known to produce fibrinolytic protease enzymes that can degrade fibrin and prevent blood clot formation. This study aimed to evaluate the differences in antithrombotic activity and hemolysis patterns of fibrinolytic protease-producing bacterial isolates obtained from the coast of Tanjung Dewa Beach, South Kalimantan. A total of 15 isolates were obtained from seawater, beach sand, mollusks, crabs, and barnacles, and then subjected to colony morphology identification, Gram staining, and spore observation. Proteolytic activity testing using Skim Milk Agar media showed that all isolates had proteolytic activity with varying indices, with PSR1 showing the highest index (3.4). Furthermore, fibrinolytic testing using the fibrin plate assay method showed that 10 isolates were capable of degrading fibrin, with AL8 and SP2 showing the highest fibrinolytic indices (3.12 and 3.11, respectively). Antithrombotic testing using the clot lysis method revealed that AL7 and SP1 exhibited the highest lysis percentages (82.05% and 88.88%, respectively). Anticoagulant activity, as determined by the Lee-White method, showed that SP2 significantly prolonged the coagulation time (142 seconds, 49.65%). Hemolysis pattern testing revealed variations in activity, with AL8 and PSR1 classified as gamma-hemolytic and therefore potentially safer. Statistical analysis showed no significant differences between isolates in terms of antithrombotic activity, anticoagulant activity, or hemolysis pattern (p > 0.05). These findings suggest that coastal bacterial isolates from Tanjung Dewa Beach, particularly AL8 and SP2, have the potential to serve as safe, natural antithrombotic agents, supporting the development of biomedical therapies for cardiovascular disease.
PERBEDAAN KUALITAS PEWARNAAN SEDIAAN SITOLOGI MENGGUNAKAN BUNGA TELANG (Clitoria ternatea L) SEBAGAI ALTERNATIF PENGGANTI METHYLENE BLUE PADA METODE DIFF QUICK Rizky Arsyanti; Neni Oktiyani; Aima Insana; Ratih Dewi Dwiyanti
Jurnal Karya Generasi Sehat Vol. 3 No. 2 (2025): Edisi Desember 2025
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v3i2.232

Abstract

Pewarnaan sediaan sitologi dilakukan untuk memperjelas morfologi sel agar dapat diamati secara mikroskopis, khususnya pada bagian inti sel dan sitoplasma. Pewarnaan Diff Quick merupakan metode yang umum digunakan karena cepat dan memiliki kontras warna yang baik, tetapi salah satu komponennya, methylene blue, bersifat toksik dan tidak ramah lingkungan. Penelitian ini bertujuan untuk mengetahui perbedaan kualitas pewarnaan sediaan sitologi menggunakan larutan bunga telang (Clitoria ternatea L) sebagai alternatif alami pengganti methylene blue dengan pewarnaan Diff Quick. Jenis penelitian True Experiment dengan rancangan Post Test Only with Control Group Design, terdiri dari 4 perlakuan dengan 6 kali pengulangan menggunakan 1 sampel cairan pleura yang diwarnai dengan larutan bunga telang pada konsentrasi 20%, 25%, dan 30%, serta dibandingkan dengan pewarnaan Diff Quick standar sebagai kontrol. Hasil pemeriksaan mikroskopik larutan bunga telang konsentrasi 20% menunjukkan kualitas tidak baik, 25% menunjukkan kualitas cukup baik dan 30% menunjukkan kualitas pewarnaan yang paling mendekati pewarnaan standar dengan kategori “sangat baik.” Uji Friedman menunjukkan perbedaan yang signifikan antar kelompok perlakuan (p=0,001), dan uji Wilcoxon menunjukkan tidak terdapat perbedaan signifikan antara larutan bunga telang 30% dengan kontrol (p=0,063). Kesimpulannya, larutan bunga telang konsentrasi 30% memiliki potensi sebagai pewarna alami alternatif methylene blue dalam Metode Diff Quick. Disarankan untuk dilakukan penelitian lebih lanjut dengan menaikkan konsentrasi larutan pewarna bunga telang atau menaikkan pH larutan pewarna bunga telang (Clitoria ternatea L).
EDUKASI DAN PELATIHAN PENGOLAHAN MINUMAN IMUNOMODULATOR PADA PENDERITA TB PARU DI WILAYAH PUSKESMAS CEMPAKA BANJARBARU Ratih Dewi Dwiyanti; Nurlailah Nurlailah; Aima Insana
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 5 No 1 (2026): Jurnal Rakat Sehat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v5i1.90

Abstract

Pulmonary TB is still a disease that is often found in the Cempaka Banjarbaru Community Health Center. Based on data from the Cempaka Health Center for January - December 2022, there are 36 tuberculosis sufferers who are still actively seeking treatment. Treatment of pulmonary TB with anti-tuberculosis drugs (OAT) takes several months so it is prone to causing side effects in sufferers. To minimize the side effects of using OAT while increasing the patient's immune system, it is necessary to consume natural ingredients (moringa, meniran and ginger) which have minimal side effects and act as immunomodulators. The aim of the activity is to increase participants' knowledge about tuberculosis and how it is transmitted as well as how to use herbal plants as immunomodulators to minimize side effects due to the use of OAT. The method used in this activity is filling out a questionnaire sheet (pretest) followed by a presentation of the material. Before the posttest was carried out, the participants were given training on how to process herbal drinks from 3 herbal ingredients, namely Moringa leaves, meniran and ginger (Kemeja) which were made in powder form for 2 hours and continued with filling out a questionnaire sheet (posttest). The results of community service can be seen based on the questionnaire sheet which shows an increase in participants' knowledge about pulmonary TB disease by 10% and behavior in preventing pulmonary TB by 10% and participants were able to make immunomodulatory drinks in the form of powder such as Kemeja (moringa, meniran, ginger). Based on the results of evaluation and monitoring, sufferers stated that their appetite increased after drinking SHIRT tea powder. It is hoped that the increase in appetite can increase the sufferer's immune system so that they can suppress the side effects of the OAT drugs they consume.
EDUKASI DAN KESADARAN MASYARAKAT TENTANG PENYAKIT JAMUR KUKU PADA PENDULANG INTAN DI CEMPAKA KOTA MADYA BANJARBARU Ratih Dewi Dwiyanti; Leka Lutpiatina; Aima Insana
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 5 No 1 (2026): Jurnal Rakat Sehat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v5i1.124

Abstract

Diamond miners are one type of job that deals daily with wet and dirty places. Diamond miners work while wearing boots, but because the mining areas are filled with water, their feet are always damp and submerged in dirty water, which supports the growth of fungus. The objective of this activity is to enhance community health knowledge about fungal nail diseases and to reduce the incidence of fungal infections by providing antifungal medication to diamond miners in the area of the Tiung River in Cempaka, Banjarbaru. The methods used in this activity included pre-tests, presentation of materials, observation, post-tests, and the administration of antifungal medication. This activity involved 29 participants consisting of 5 females and 24 males. The results of the community service showed increased knowledge among partners regarding fungal infections of the nails based on the questionnaire results. As a result of the observation, it was found that all participants showed symptoms of fungal infection in their toe nails. Monitoring and evaluation were conducted 3 weeks after the counseling and observation activities, during which antifungal medication was distributed. It was concluded that the counseling activities could enhance the knowledge of the diamond miners
Comparative Antibacterial Activity of Citrus hystrix DC. Leaf Essential Oil Against ESBL Klebsiella pneumoniae and Multidrug-Resistant Serratia marcescens Leka Lutpiatina; Norsyifa Norsyifa; Mahira Ainiyah Putri; Ratih Dewi Dwiyanti; Aima Insana
Tropical Health and Medical Research Vol. 8 No. 2 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The increasing antimicrobial resistance has driven the development of natural antibacterial sources, one of which is essential oils. This study aimed to analyse the antibacterial activity of lime leaf essential oil (Citrus hystrix DC.) against Extended Spectrum ?-Lactamase (ESBL)-producing Klebsiella pneumoniae and multidrug-resistant Serratia marcescens. The study used a true experimental design with a posttest-only control group design. The essential oils were tested at concentrations of 50%, 60%, 70%, 80%, 90%, and 100% using the well diffusion method with four replications. Ciprofloxacin served as a positive control and dimethyl sulfoxide as a negative control. Data were analysed using the Shapiro–Wilk test, Levene's test, one-way ANOVA, or Welch ANOVA, followed by post hoc tests according to data characteristics at a significance level of p<0.05. The results showed that lime leaf essential oil had antibacterial activity against both bacteria at all concentrations tested. For Klebsiella pneumoniae ESBL, the inhibition zone diameter increased from 6.83 mm at 50% concentration to 9.30 mm at 90% concentration, then decreased to 8.08 mm at 100%. For multidrug-resistant Serratia marcescens, the inhibition zone diameter increased from 7.80 mm to 14.00 mm at 90% concentration, then decreased to 11.20 mm at 100%. Statistical analysis showed a significant difference between the concentrations for both bacteria (p<0.001). In general, Serratia marcescens showed higher sensitivity than Klebsiella pneumoniae ESBL. Although its inhibitory power was still lower than ciprofloxacin, lime leaf essential oil has the potential to be developed as a natural antibacterial source against resistant Gram-negative bacteria.
Antibacterial Activity of Limau Kuit (Citrus hystrix DC.) Leaf Essential Oil Against Methicillin-Resistant Staphylococcus aureus and Multidrug-Resistant Pseudomonas aeruginosa Leka Lutpiatina; Fitria Audrey Mau'izah; Wina Fitria; Ratih Dewi Dwiyanti; Aima Insana
Tropical Health and Medical Research Vol. 8 No. 2 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Antibiotic resistance in pathogenic bacteria, including Methicillin-resistant Staphylococcus aureus (MRSA) and Multi-drug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa), has encouraged the development of alternative antibacterial agents from natural sources. This study aimed to analyse the antibacterial Activity of Limau Kuit (Citrus hystrix DC.) leaf essential oil against MRSA and MDR P. aeruginosa. The study used a true experimental design with a posttest-only control group design. The essential oil was obtained through steam-water distillation of Limau Kuit leaves and then tested at concentrations of 50%, 60%, 70%, 80%, 90%, and 100% using the well diffusion method. Positive controls included ciprofloxacin and ceftazidime, while dimethyl sulfoxide served as the negative control. Each treatment was repeated four times. Data were analysed using Welch ANOVA and Games-Howell tests for MRSA and Kruskal-Wallis and Mann-Whitney tests for MDR P. aeruginosa. The essential oil yield was 0.60% (v/w). All concentrations showed antibacterial activity against both test bacteria. The largest inhibition zone diameter against MRSA was obtained at 90% (22.23 ± 1.75 mm), while against MDR P. aeruginosa at 80% (13.98 ± 1.48 mm). Statistical analysis showed a significant difference between concentrations in MRSA (p < 0.001) and MDR P. aeruginosa (p = 0.003). Antibacterial activity against MRSA is higher than against MDR P. aeruginosa, which is thought to be related to differences in cell wall structure and resistance mechanisms of the two bacteria. Lime leaf essential oil has potential as a natural antibacterial against antibiotic-resistant bacteria. However, further research is needed to determine active compound content, mechanism of action, and the minimum inhibitory concentration and minimum bactericidal concentration.
Co-Authors Agnina Listya Anggraini Ahmad Muhlisin Ahmad Muhlisin Aima Insana Aina Nurvita Sari Aisya Nahdiya Akhmad Muhlisin akhmad muntaha Amalia Amalia Amperawati , Metty Anggraini, Agnina Listya Annisa Nur Yulianti Anny Thuraidah Anny Thuraidah Anny Thuraidah, Anny Ayu fahani Ayu Sutiany Azmi, Dini Aulia Bahrul Ilmi Barkinah , Tut Deby Arnisya Putri Dini Aulia Azmi Dinna Rakhmina Dinna Rakhmina Ekrima Dayanti Erfan Roebiakto Erpan Roebiakto fahani, Ayu Fitria Audrey Mau'izah Fransiskus Xaverius Agung Pinto Laksono Hafidh, Muhammad Haitami Haitami haitami haitami, haitami hana nailah Haris Nurdin Pratama Indah Kurnia Widiningsih Ipniliati, Eva Irfai, Irfai Junaidi Junaidi Khair , Abdul Leka Lutpiatina leka lutpiatina, leka Mahira Ainiyah Putri Mahpolah Mahpolah, Mahpolah Mufid, Muhammad MUHAMMAD RIZAL Neni Oktiyani Norsyifa Norsyifa Nur Asy Syifa Azizah Nur Lailah Lailah Nur Rizka Adriana Nur Rizqi Amaliah Nurhamidi , Nurhamidi Nurlailah Nurlailah Nurlailah Nurlailah Nurlailah Nurlailah, Nurlailah Nurmalasari Nurmalasari Nurul Huda Nurul Huda Pratama, Haris Nurdin Putri Wulandari, Rima Rahmayunita Rahmayunita Rahmayunita Rifqoh Rifqoh Rima Putri Wulandari Rion Dediq Rizkiyah, Risa Rizky Arsyanti Sa'adah, Sa'adah Siti Mas'odah Suroto Suroto Sutiany, Ayu Sya’adah, Nurul Laili Ulfah, Putriana Utama, Ricko Dharmadi wahdah norsiah Wahdah Norsiah, Wahdah Wina Fitria Yuli Susanti Yuli Susanti