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All Journal Medical Laboratory Technology Journal Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) UMI Medical Journal Ebers Papyrus Journal Of Health Science Community
Mansyur Arif
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Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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ESTIMATED BLOOD LOSS IN OPEN HEART SURGERY (Taksiran Kehilangan Darah di Bedah Jantung Terbuka) Riesti Ekasanti; Rachmawati Muhiddin; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1232

Abstract

Taksiran kehilangan darah merupakan perkiraan kehilangan darah di setiap tindakan pembedahan. Permintaan darah untukpersiapan pembedahan elektif sering tidak sesuai dengan jumlah darah yang ditransfusikan selama tindakan tersebut karena belum adapanduan mengenai jumlah permintaan darah untuk tata langkah bedah. Penelitian ini bertujuan untuk mengetahui taksiran kehilangandarah pada pembedahan jantung terbuka dan kesesuaian dengan permintaan darah dalam kegiatan tersebut. Penelitian dilakukan secaraobservasional retrospektif seraya mengambil data rekam medis di Instalasi Rekam Medik RSUP dr. Wahidin Sudirohusodo Makassarantara bulan Januari 2009−Desember 2012. Sebanyak 32 pasien yang menjalani bedah jantung terbuka didapatkan dengan presentasilaki-laki sebanyak 22 pasien (68,75%) dan perempuan sebanyak 10 orang (31,25%). Jumlah taksiran kehilangan darah bedah jantungterbuka rerata 2,80 kantong darah lengkap (980 mL) dan permintaan darah untuk kegiatan tersebut rerata sebanyak 4,00 kantong(1400 mL). Antara jumlah permintaan darah persiapan bedah dan taksiran kehilangan darah (p=0,149) terdapat ketidaksesuaian.Taksiran kehilangan darah di bedah jantung terbuka di RSUP dr. Wahidin Sudirohusodo Makassar di RSUP dr. Wahidin SudirohusodoMakassar adalah 2,80 kantong darah lengkap (980 mL), yang tidak sesuai dengan jumlah permintaan darah persiapan bedah.
PEMERIKSAAN PROTHROMBIN TIME DAN ACTIVATED PARTIAL THROMBOPLASTIN TIME DENGAN HUMACLOT VA SERTA SYSMEX CA 500 Misnah Misnah; Agus Alim Abdullah; Mansyur Arif; Burhanuddin Bahar
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.382

Abstract

Prothrombin Time (PT) and an activated Partial Thromboplastin Time (aPTT) are routine coagulation tests used for pre-operative screening. The analytical step as one of the laboratory test’s stage that plays the role in the determination of the test is influenced by several factors, one of them is choosing its proper devices. The aim of this study was to know the correlation of the PT and aPTT test’s result using Humaclot VA and Sysmex CA 500 devices. A cross sectional study has been done at the Clinical Pathology Laboratory of Wahidin Sudirohusodo Hospital Makassar started from May 2009 until June 2009. The data were analyzed with T and Pearson’s Correlation test. From the 50 samples were obtained the percentage of the corresponding frequency of the PT results between Humaclot VA and Sysmex CA 500 about 84%, whereas the frequency of the corresponding results aPPT between Humaclot VA and Sysmex CA 500 is 76%, the Pearson correlation test for PT=0.58, and aPTT=0.38. There were found the suitability of PT, aPTT of Humaclot VA with CA 500 and both tools have a positive correlation.
ANALISIS TES IMUNOKROMATOGRAFI DAN ENZYME-LINKED IMMUNOSORBENT ASSAY UNTUK MENDETEKSI Helicobacter pylori DI PASIEN DISPEPSIA I Hutagalung; Uleng Bahrun; Mansyur Arif; Rifai Amirudin; HAM Akil
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.959

Abstract

Many methods are available to diagnose Helicobacter pylori in patients with dyspepsia, including Enzyme-Linked ImmunosorbantAssay (ELISA) and recently used is immnochromatography test. Both of the test are non invasive method, but immunochromatographytest can be used in laboratory without semi automatic analyzer. The aim of this study was to compare the immunochromatographyand ELISA tests in detecting the possibility of Helicobacter pylori infection. A cross sectional study was done among 49 sampleswith dyspepsia at Wahidin Sudirohusodo Hospital of Makassar and Prodia Laboratory from March to August 2008. Samples wereanalyzed with SPSS 14 for Windows Program using Chi Square and Spearman correlation tests. Among 49 samples we found ELISAand immunochromatography tests were positive in 5 samples, ELISA and immunochromatography tests were negative in 33 samples,ELISA tests were borderline and immunochromatography tests were positive in 5 samples, and ELISA tests were borderline andimmunochromatography tests were negative in 6 samples. There was a good correlation between ELISA and immunochromatographytest with R = 1.000 and p = 0.000. Both results of the immunochromatography and ELISA tests showed high conformity. Both methodcan be applied to diagnose Helicobacter pylori in patients with dyspepsia. Immunochromatography test can be an alternative methodin laboratories who do not apply semi automatic analyzer. The advantages of immunochromatography test can be used for diagnoseearly infection of Helicobacter pylori.
UJI HEMATOLOGI PASIEN TERDUGA DEMAM BERDARAH DENGUE INDIKASI RAWAT INAP Juranah .; Darwati Muhadi; Mansyur Arif; Burhanuddin Bahar
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1169

Abstract

Indonesia is an endemic area of Dengue Hemorrhagic Fever (DHF). The clinical symptoms of this disease varies; it can beasymptomatic or atypical fever. Therefore, the early detection of Dengue virus is important to reduce the incidence of new victims fromhis infection. The aim of this study was to find out the haematological features of DHF suspected patients indicated for hospitalization. Aross-sectional study was done on DHF suspected patients indicated for hospitalization. The data were obtained from the Medical Recordat Wahidin Sudirohusodo Hospital from January-July 2009. The data were presented in the form of table. The results showed that themean leukocyte counts was 5.138 103/µL ranging from 2.10–16.70 103/µL, the mean erythrocyte count was 5.136 106/µL rangingrom 3.2–6.40 106/µL, the mean platelet count was 111.27 103/µL ranging from 11.40–260 103/µL. The DHF patients at admissionwho had leucositopenia, thrombocytopenia, and monocytosis were 54.30%, 71.40%, and 51.40%, respectively. Based on the result itan be concluded, that the most common haematological abnormality in DHF suspected patients whom indicated for hospitalizationwere thrombo cytopenia, leucocytopenia and monocytosis.
THE MORPHOLOGICAL FEATURES OF ERYTHROCYTES IN STORED PACKED RED CELLs Dewi Sri Kartini; Rachmawati Muhiddin; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1128

Abstract

Morfologi eritrosit Packed Red Cells (PRC) akan mengalami perubahan selama penyimpanan di suhu 2°–8°C. Eritrosit dalammempertahankan viabilitasnya membutuhkan adenosine triphosphate (ATP). Apabila kadar ATP intraseluler menurun, terjadi kerusakanlipid membran, penumpukan Natrium dan Kalsium intraseluler, penurunan kadar Kalium dan air intraseluler, dehidrasi sel, membranmenjadi kaku dan bentuknya berubah dari cakram menjadi sel krenasi, sferosit dan bite cell. Penelitian ini bertujuan untuk melihatpersentasi bentuk eritrosit crenated cell, sferosit dan bite cell PRC simpan pada hari ke-3 yang digunakan sebagai pembanding, hari ke-7,ke-14 dan ke-21 dari tanggal aktaf kantong darah. Penelitian observasional dengan pendekatan kajian kohort dilakukan pada bulanAgustus 2015 di BDRS RSUP Dr. Wahidin Sudirohusodo Makassar. Sampel sebanyak 30 selang kantong darah menjadi 120 hapusandarah slide. Dari 30 sampel kantong darah dengan golongan darah A 26,6%, B 13,3%, AB 16,6% dan O 43,3%, didapatkan peningkatanpersentase jumlah crenated cell, sferosit dan bite cell setelah penyimpanan hari ke-3, ke-7, ke-14 dan ke-21. Penyimpanan hari ke-3dijadikan pembanding. Data diolah dengan menggunakan uji Fiedman dan Wilcoxon dengan nilai kemaknaan p<0,001. Terdapatpeningkatan persentase perubahan morfologi eritrosit (crenated cell, sferosit dan bite cell) seiring dengan lamanya penyimpanan darahPRC. Pemakaian darah PRC dianjurkan tidak boleh melebihi 21 hari penyimpanan.
HUBUNGAN DERAJAT PERLEMAKAN HATI NON-ALKOHOLIK DENGAN AKTIVITAS AMINOTRANSFERASE SERUM Nyoman Trisna Yustiani; Mutmainnah .; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.1027

Abstract

Fatty liver could be related to alcoholic or non-alcoholic. Mild to moderate elevation of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities are the most laboratories test ordered in patient with non-alcoholic fatty liver. This studyhas purposed to know the relation of degree non-alcoholic fatty liver’s with aminotransferase serum activity. A cross-sectional studywas done in 38 patients with non-alcoholic fatty liver aged 30 to 60 years at Clinical Pathology Laboratory Installation and RadiologyDepartment Dr. Wahidin Sudirohusodo Hospital in Makassar from March to July, 2008. Patient made to criteria done some laboratorytest for AST and ALT examination. Data was analyzed using T-Test in SPSS for windows version 11,5. In this study we found that therewere a significant difference between non-alcoholic fatty liver degree with AST and ALT activity in cutt off 18 (p < 0,05). From this studywe could conclude that the activity of AST and ALT serum can be used to predict probability of non-alcoholic fatty liver.
DIAGNOSIS TIROID (Diagnosis of Thyroid) Liong Boy Kurniawan; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1285

Abstract

Thyroid disease often causes unspecific or mild symptoms, so laboratory tests are needed to confirm the functional diagnosis of thethyroid disorder. The laboratory tests which are important to establish the diagnosis of thyroid disorder include: total and free thyroidhormones, its related (thyroid) hormone binding proteins and auto antibodies. The thyroid hormone tests are mostly measured withcompetitive or sandwich immunoassays and each method can be interfered by several factors. Some drugs may increase or decrease thethyroid functional tests and several factors such as: underlying diseases, age, pregnancy, occurrence of heterophil antibody and autoantibodies may also interfere the thyroid tests results. The interpretation of an unusual combination from thyroid stimulating hormonesuch as free thyroxin and tri-iodothyronine results needs confirmation of underlying condition for establishing the right diagnosis. Thisreview is aimed to evaluate several factors which may influence the thyroid tests and interpretation.
IMUNOGLOBULIN A DI DEMAM BERDARAH DENGUE Iwan Joseph; Uleng Bahrun; Idham Jaya Ganda; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 1 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i1.350

Abstract

Recently several serology tests have been used to detect IgM and IgG antidengue, and NS1 antigen. On the last study was launched a new diagnose test using immunochromatography method to detect the specific antidengue IgA antibody. The aim of this study was to evaluate the IgA antidengue performance using immunochromatography method in Dengue Hemorrhagic Fever patients. The study was carried out during March up to July 2010 by a cross sectional method. The samples taken were 1–14 year‘s old patients from the Infectious Tropical Sub Division of the Department of Paediatric, Dr. Wahidin Sudirohusodo Hospital, Makassar. The samples were diagnosed Dengue Hemorrhagic Fever based on the criteria of WHO 1997 and they also subjected to IgM and IgG serology tests as well. The test for IgA is using immunochromatography method. The data was analyzed by Spearman Correlation Test. From the total 40 samples, which consist of 16 (40%) male and 24 women (60%), generally were taken on the fourth day until the sixth day of onset. There were more IgA anti Dengue detected rather than IgM on the secondary infection. The researchers found moderate correlation among the IgA test with IgM (+), IgG (+), or both positive. Based on this study so far it can be concluded that the Dengue IgA test can be used to detect the primary or secondary Dengue virus infection.
CREATINE KINASE RELATED TO THE MORTALITY IN MYOCARDIAL INFARCTION (Creatine Kinase terhadap Angka Kematian di Infark Miokard) Liong Boy Kurniawan; Uleng Bahrun; Darmawaty Rauf; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1178

Abstract

Creatine Kinase (CK) merupakan salah satu uji yang sering diperiksakan untuk mendiagnosis infark miokard akut. Peningkatanenzim ini menunjukkan ada nekrosis sel otot meskipun tidak khas di otot jantung. Kenaikan tingkat CK di infark miokard akutmenggambarkan luas serta beratnya penyakit dan dihubungkan dengan peningkatan angka kematian pasien. Untuk mengetahui tingkatCK di pasien infark miokard akut saat masuk rumah sakit dan menilai pengaruhnya terhadap angka kematian pasien selama perawatandi rumah sakit. Penelitian ini merupakan kajian potong lintang dengan mengambil data sekunder dari rekam medik 62 pasien infarkmiokard akut yang dirawat di Unit Perawatan Jantung Intensif Rumah Sakit Dr. Wahidin Sudirohusodo Makassar masa waktu bulanJuli 2010 hingga Juni 2011. Kadar CK yang diteliti diperoleh saat pasien masuk rumah sakit. Uji statistic dilakukan dengan uji MannWhitney, T Test dan Chi-Kuadrat. Rerata kadar CK di pasien infark miokard akut yang tetap hidup dan meninggal selama perawatanadalah 507,38+749,62 U/L dan 1995,39+2290,06 U/L (p=0,002). Perbedaan tingkat angka kematian yang bermakna ditemukanantar kuartil CK (p=0,021). Kebahayaan relatif angka kematian pada kuartil keempat (4) sebesar 13 kali dibandingkan dengan kuartilkesatu (1) (selang kepercayaan 95%, 2,07-81,5; p=0,006), kuartil ketiga (3) sebesar 4,8 kali dibandingkan dengan kuartil kesatu (1)(selang kepercayaan 95%, 1,07-21,45; p=0,04) dan kuartil kedua (2) sebesar 3 kali dibandingkan dengan kuartil kesatu (1) (selangkepercayaan 95%, 0,68-13,3; p=0,148).Didasari telitian ini, ditemukan perbedaan bermakna kadar CK ditemukan saat masuk rumahsakit di pasien infark miokard akut yang tetap hidup maupun yang meninggal selama perawatan. Semakin tinggi kadar CK saat pasienmasuk rumah sakit semakin tinggi tingkat angka kematiannya.
ANALISIS EOSINOFIL DARAH TERKAIT RADANG SEL GINJAL AKUT/ NEFRITIS INTERSTISIAL AKUT (NIA) Yedid Lebang; Sulina Yanti Wibawa; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.964

Abstract

Acute Interstitial Nephritis (AIN) is the main cause of acute renal failure because of hypersensitivity to many antibiotics and otherdrugs that potent to induce allergic response in renal interstitial tissue. Gold standard for AIN diagnosis upheld from clinical sign,laboratory, USG, Gallium renal tests and renal biopsy. A cross sectional study was carried out among 120 (AIN) patients and none (AIN)from the Paediatrics and Internal Medicine Department Wahidin Sudirohusodo hospital from June until September 2008. Eosinophilcount was performed using haematology auto analyser. The data was analyzed by Mann-Whitney test using SPPS for windows. Thetotal 120 samples consisted of 60 samples of AIN and 60 samples Non AIN were included in this study. Mean eosinophil in AIN 4. 9 %and non AIN 3. 1%. There were statistical difference of eosinophil level between AIN and non AIN with p < 0. 00. Eosinophil level canbe used to differentiated between AIN and non AIN in conjunction to the clinical sign.
Co-Authors Abd Muis Agus Alim Abdullah Ani Kartini Arie Yanti Asvin Nurulita Baharuddin Baharuddin Burhanuddin Bahar Cyntia Kornelius D. Daud Darmawaty Darmawaty Rauf Darwati Muhadi Dewi Sri Kartini Fitrie Octavia Glent Nurtanio HAM Akil Hardjoeno Hardjoeno Hendra Rasubala herlinah herlinah Hilma Yuniar Hilma Yuniar Thamrin Hj. Darmawaty E.R, I Hutagalung I. Hutagalung Ibrahim Abd. Samad Ibrahim Abdul Samad Ibrahim Labeda Ichwan Meinardi Ichwani Meinard Idham Jaya Ganda Ilhamjaya Patellongi Irawaty Irawaty Irfan Idris Irna Diyana Kartika Iwan Joseph Joko Widodo Juranah . Kusriny A.P. Lince Wijoyo Liong Boy Kurniawan, Liong Boy Muh Taufiq Muh Taufiq Mutmainnah . Nur Rahmi Raehaan Nurhayana Sennang Nursin Abd. Kadir Nyoman Trisna Yustiani Rachmawati A.M Rachmawati AM Rachmawati Muhiddin Rachmawati Muhiddin Raehana Samad Reini Meilani Isbach Riesti Ekasanti Rifai Amirudin Rima Yuliati Muin Rostina Rostina Ruland Pakasi Sri Wahyunie S Suci Aprianti Sukmawaty Sukmawaty Sulina Yanti Wibawa Suriyanti Suryani Jamal Susi Sevianty Syahraswati Syahraswati Theosobia Grace Orno Uleng Bahrun Viviyanti Zainuddin Waode Rusdiah Winni Agustiani Wiwi Payung Yedid Lebang Yosep F. Tallulembang ZK Abdurahman Baizal