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Journal : BIOMA

LIPASE AND PROTEASE LEVELS ON KOMBUCHA FERMENTATION Endah Rita S. D.
Bioma : Jurnal Ilmiah Biologi Vol 2, No 1, April (2013): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v2i1, April.400

Abstract

Processed tea is fermented Kombucha Tea is known as "(Kombucha Tea). Kombucha tea is a functional food which occupies a position somewhere between conventional foods and drugs, so it can be used in the prevention of a disease of functional foods are foods that are designed specifically to take advantage of certain bioactive compounds that have a role in preventing disease.The enzyme is part of a protein that acts as a biocatalyst, accelerate the rate of biochemical reactions in the body. Therefore, the enzyme can be able to improve the health functions of the body with Kombucha.The purpose of this study (1) Knowing the production of lipase and protease in fermentation of Kombucha tea with many type, (2) Knowing the best kinds of tea for lipase and protease levels are optimal.The method used in this study is an experimental method to the design of Completely Randomized Design. This study consisted of four treatments, namely (A) + Kombucha colony of tea without sugar solution, (B) + Kombucha colony of black tea, (C) + Kombucha colony of green tea and (D) + Kombucha colony of fragrant tea (jasmine tea) with 3 replications. The parameters are count lipase and protease levels.Data analysis was performed with Analysis of Variance (ANOVA), followed by Duncan Multiple Test Distance (UJGD). ANOVA from lipase production in Kombucha fermentation of tea with significant variations, as well as protease levels is significant.The results showed that the type of tea as a fermentation medium had a significant influence (p <0.05) on levels of lipase and protease produced during the fermentation process and Kombucha tea as fermentation mediaKey words: Lipase, Protease, fermented Kombucha mushroom, tea variation
PENERAPAN MEDIA VIRTUAL LABORATORIUM DALAM PEMBELAJARAN BIOLOGI MATERI EUBACTERIA TERHADAP HASIL BELAJAR DAN KARAKTER SISWA Endah Rita Sulistya Dewi; Prasetiyo Prasetiyo
Bioma : Jurnal Ilmiah Biologi Vol 4, No 1 (2015): April 2015
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v4i1, April.948

Abstract

Virtual Laboratory Learning could be used to eliminate the limitationsof laboratory devices. A mix between the computer software which isdesigned to represent an alternative implementation concept of VirtualLaboratory. Through KD 3.4. Applying the Classification to classifyarchaebacteria and eubacteria based on characteristics and shapethrough systematic observation. It selected the Gram staining processin bacteria, which is to find groups of bacteria including GramPositive or Negative. The media influenced learning process in manysection, it is possible that the virtual lab used as a means to internalizethe values of character. This research?óÔé¼Ôäós aim was to producedLaboratory Virtual Media Instruction, which was oriented formcharacter building through the development process. The method wasused research and development refers to the flow of Borg and Gall(1989). The results showed increasement in postal value, which isused Laboratory Virtual Media, compared to pre test, but the N-gainwas quite low at 0.2. Dicipline character, cooperation, honesty andaccuracy in good categories.Keywords: Virtual Laboratory, Eubacteria
PENURUNAN KONSENTRASI LOGAM BERAT KADMIUM (Cd) DAN PERTUMBUHAN MIKROALGA Chlorella vulgaris PADA MEDIA KULTUR Puput Widiyani -; Endah Rita Sulistya Dewi
Bioma : Jurnal Ilmiah Biologi Vol 3, No 2, Oktober (2014): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v3i2, Oktober.640

Abstract

DECREASE IN THE CONCENTRATION OF HEAVY METAL CADMIUM (Cd) AND MICROALGAE chlorella vulgaris GROWTH ON CULTURE MEDIA ABSTRACT The objectives of this study is to determine the decrease in heavy metal concentrations of Cd in the culture media and to determine the effect of Cd concentration on the growth of Chlorella vulgaris. This study used a completely randomized design with four treatments and three repititions. This treatment includes, K treatment (control), treatment Cd 1 (cadmium concentration addition 1 mg / L), treatment Cd 3 (cadmium concentration addition 3 mg / L) and treatment Cd 5 (cadmium concentration addition 5 mg / L). Each sample was analyzed by AAS. Data were analyzed by using analysis of variance that was continued by Duncan's test. The results of the analysis of metal concentrations decrease of Cd in the culture medium is Cd 5 (0.4494 mg / l), Cd 3 (0.4008 mg / l), Cd 1 (0.2658 mg / l), and K (0.1162 mg / l). The analysis result of F value of variance showed that Chlorella vulgaris gave highly significant effect in decreasing the concentration of Cd. Whereas the result of the growth of Chlorella vulgaris during culture is Cd 3 (1015.97 cells / ml), Cd 1 (769.70 cells / ml), Cd 5 (719.55 cells / ml), and K (668.21 cells / ml ). The result of analysis variance showed that the various concentrations of Cd gave significant effect on the growth of Chlorella vulgaris. The conclusion is that Chlorella vulgaris have ability as bioremediator cadmium with different concentrations, this is indicated by the reduced content of Cd in the culture medium. The ability of metal Cd can substitute Zn function in synthesizing enzyme carbonic anhydrase that causes maximal growing. ?é?á Keywords: Decreased concentration, cell growth, cadmium (Cd), Chlorella vulgaris ABSTRAK Penelitian ini bertujuan untuk mengetahui penurunan konsentrasi logam berat Cd pada media kultur dan untuk mengetahui pengaruh konsentrasi Cd terhadap pertumbuhan Chlorella vulgaris. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 kali pengulangan. Perlakuan ini meliputi, perlakuan K (kontrol), perlakuan Cd 1 (penambahan konsentrasi kadmium 1 mg/L), perlakuan Cd 3 (penambahan konsentrasi kadmium 3 mg/L) dan perlakuan Cd 5 (penambahan konsentrasi kadmium 5 mg/L). Masing-masing sampel dianalisa dengan AAS. Analisa data menggunakan analisis variansi yang dilanjutkan dengan uji Duncan. Hasil analisis penurunan konsentrasi logam Cd pada media kultur adalah Cd 5 (0,4494 mg/l), Cd 3 (0,4008 mg/l), Cd 1 (0,2658 mg/l), dan K (0,1162 mg/l). Hasil analisis varians F hitung menunjukkan bahwa Chlorella vulgaris memberikan pengaruh berbeda sangat nyata terhadap penurunan konsentrasi logam Cd. Sedangkan hasil pertumbuhan Chlorella vulgaris selama kultur adalah Cd 3 (1015,97 sel/ml), Cd 1 (769,70 sel/ml), Cd 5 (719,55 sel/ml), dan K (668,21 sel/ml). Hasil analisis varians menunjukkan bahwa berbagai konsentrasi logam Cd memberikan pengaruh berbeda nyata (signifikan) terhadap pertumbuhan Chlorella vulgaris. Kesimpulannya ialah Chlorella vulgaris memiliki kemampuan sebagai bioremediator kadmium dengan konsentrasi yang berbeda, hal ini ditunjukkan dengan berkurangnya kandungan Cd dalam media kultur. Kemampuan logam Cd dapat menggantikan fungsi Zn dalam mensintesis enzim karbonik anhidrase yang menyebabkan pertumbuhan semakin maksimal. ?é?á Kata?é?á kunci : Penurunan konsentrasi, pertumbuhan sel, kadmium (Cd), Chlorella vulgaris
BIOMONITORING PENCEMARAN SUNGAI SILUGONGGO KECAMATAN JUWANA BERDASARKAN KANDUNGAN LOGAM BERAT (Pb) PADA IKAN LUNDU Lilik Chauro Aina; Endah Rita S.D.; Fibria Kaswinarni
Bioma : Jurnal Ilmiah Biologi Vol 5, No 2 (2016): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v5i2.1498

Abstract

Biomonitoring adalah pemanfaatan organisme untuk memonitor dan menilai/ mendeteksi kondisi suatu lingkungan. Sungai Silugonggo merupakan sungai terbesar di Kecamatan Juwana, yang berfungsi sebagai tempat bersandarnya kapal, MCK, TPI, serta industri yang berpotensi membuang limbah mengandung Pb yang berpengaruh pada kondisi fisik kimia dan organisme yang berada di sungai. Penelitian ini bertujuan untuk mengetahui kondisi fisik kimia dan kandungan logam berat timbal (Pb) pada ikan Lundu (Mystus nigriceps) di perairan Sungai Silugonggo Kecamatan Juwana. Penelitian ini menggunakan metode survey. Pengambilan sampel air dan ikan dilakukan pada 3 stasiun, yaitu stasiun pertama Desa Doropayung, stasiun kedua Desa Kauman, dan stasiun ketiga Desa Bajomulyo. Pengambilan sampel dilakukan secara random dengan jarak 100 meter antar titik. Parameter yang diukur meliputi sifat fisik (suhu, kecepatan arus, kecerahan air, kedalaman sungai), sifat kimia (pH, COD, BOD, DO), kandungan logam Pb pada air dan Ikan Lundu. Kondisi Fisik dan Kimia didapatkan hasil antara lain: suhu air Sungai Siugonggo berkisar 280C-29,330C dengan baku mutu deviasi 3COD berkisar 11,3-17,40 ppm dengan baku mutu 25ppm, BOD berkisar 3,68-5,22ppm dengan baku mutu 3ppm. Pengujian Logam Berat Timbal (Pb) pada air dan Ikan Lundu dilakukan di laboratorium Universitas Diponegoro Semarang. Hasil uji kandungan logam timbal (Pb) pada air adalah 0,026-0,056ppm, hasil tersebut melampaui baku mutu air PPRI No.82 Tahun 2001 kelas II yaitu 0,003ppm. Hasil uji kandungan logam timbal (Pb) pada Ikan Lundu 0,497-0,725, hasil tersebut masih dibawah baku mutu BPOM Nomor 03725/B/SK/VII/89 yaitu 2,0ppm.Kata kunci : Biomonitoring, Sifat Fisik Kimia, Logam Timbal (Pb), Ikan
THE USE OF Cymbopogon nardus AS Aedes aegypti LARVASIDA Endah Rita S.D.; Dewi Ratna Ningtyas
Bioma : Jurnal Ilmiah Biologi Vol 1, No 1, April (2011): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v1i1, April.79

Abstract

ABSTRACT Control of mosquitoes as vectors of disease is generally performed using synthetic pesticides, with all its negative impacts. So it takes an effort to obtain alternative materials which are more environmentally friendly but also effective in controlling insects that is botanical pesticides. This study aims at: 1) determining the correct dosage of ?é?áleaves extracts and stem of citronella as a botanical pesticide exterminator of?é?á Aedes aegypti mosquito larvae; ?é?á2) determining LD50 extracts of leaves and stems of citronella and safe limits for non target organisms. This research was done in Salatiga B2P2VRP Laboratory and the Laboratory of Organic Chemistry, Department of Chemistry, Faculty of Science, University of Diponegoro. The method used is a bioassay (biological assay) , i.e. acute toxicity testing of chemicals using the or-ganism as a test animal. Subjects were Aedes aegypti three sub stadium (instar) as test target animals, and Goldfish (Carassius auratus) as test animals are not the target. Data obtained from test animals actually target and non target animals were analyzed and interpreted by a simple linear regression analysis. The result showed?é?á that ?é?áLD100 Aedes aegypti larvae mortality was ?é?á24575 ppm, and the LD50 is amounted to 35000 ppm, then the safe limit for other non target organism (environment) is 3500 ppm. The conclusion is that citronella (Cymbopogon nardus) can be used as a botanical pesticide with a safe margin for other organisms is 3500 ppm. Key words: Cymbopogon nardus, larvasida, Aedes aegypti, LD50, Carassius auratus.
PENGARUH MODEL PEMBELAJARAN TGT MENGGUNAKAN MEDIA PUZZLE TERHADAP KEAKTIFAN DAN HASIL BELAJAR KOGNITIF SISWA PADA MATERI SISTEM EKSKRESI SISWA KELAS VIII MTS N 1 SEMARANG Ina Kristiana; Atip Nurwahyunani; Endah Rita Sulistya Dewi
Bioma : Jurnal Ilmiah Biologi Vol 6, No 2 (2017): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v6i2.1740

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh model pembelajaran TGT (Teams Games Turnament) menggunakan media puzzle terhadap keaktifan dan hasil belajar siswa pada materi sistem ekskresi manusia kelas VIII MTs Negeri 1 Semarang Tahun 2016/2017. Bentuk penelitian eksperimen berdesain “quasi experimental design dengan menggunakan metode pretest-posttest control group design”. Teknik pengambilan sampel menggunakan teknik cluster random sampling. Populasi dalam penelitian ini adalah siswa kelas VIII MTs Negeri 1 Semarang. Metode pengumpulan  berupa metode observasi, dokumentasi dan tes. Analisis data untuk menguji efektifitas pembelajaran dengan menggunakan uji t dan persentase ketuntasan belajar klasikal. Hasil penelitian menunjukan ada perbedaan signifikan pada kelas eksperimen dan kelas kontrol, persentase ketuntasan belajar kelas eksperimen 76,66% sedangkan kelas kontrol 54,54%, rata-rata kelas eksperimen sebesar 75,83 sedangkan kelas kontrol 72,27. Hasil uji kolerasi dengan  = 1,835 dan  = 1,67 karena  >  maka  diterima. Untuk keaktifan belajar ditunjukkan dari rata-rata kelas eksperimen 80,57 sedangkan kelas kontrol 56,55. Hasil kolerasi dengan  = 8,168 dan  = 1,67. Disimpulkan bahwa penggunaan model pembelajaran TGT menggunakan media puzzle dapat meningkatkan keaktifan dan hasil belajar siswa pada materi sistem ekskresi manusia kelas VIII MTs Negeri 1 Semarang. Kata Kunci : Teams Games Turnament, Keaktifan, Hasil Belajar, Sistem Ekskresi Manusia 
INFLUENCE GIVING OF EXTRACT FRUIT MENGKUDU TO HISTOPATOLOGI TESTIS WHITE MOUSE FOLLOWING AN INHALATION CIGARETTE SMOKE Endah Rita S. D.
Bioma : Jurnal Ilmiah Biologi Vol 1, No 2, Oktober (2011): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v1i2, Oktober.413

Abstract

This Research to know influence giving of extract fruit Mengkudu (Morinda citrifolia) to histopatologi of white mouse (Rattus norvegicus) testis following an inhalation cigarette smoke and to know how much is giving of extract fruit of Mengkudu more having an effect on in an optimal fashion for the minimization of to damage of testicle seminiferus tubulus white mouse given by wistar galur cigarette smoke treatmen.Eksperiment design in this research use completely random design (RAL) by four treatment that is (A) mouse + cigarette smoke + 0 ml / fruit extract day of Mengkudu as control; (B) mouse + cigarette smoke + 0,9 ml / fruit extract day of Mengkudu; ( C) mouse + cigarette smoke + 1,8 ml / fruit extract day of Mengkudu; (D). mouse + cigarette smoke + 2,7 ml / fruit extract day of Mengkudu, by four replication. Para-meter the measured white mouse testis seminiferus tubulus histopatologi. Research executed by UNNES in January 2011 until March 2011.Result of research indicate that treatment of fruit extract of mengkudu give influence which signifikan ( p< 0,05) to white mouse testis seminiferus tubulus histopatologi of wistar galur. Usage of fruit extract of Mengkudu dose 1,8 ml / day optimal dose in minimization damage of white mouse testis seminiferus tubulus, following an inhalation cigarette smoke.From result of the research can be concluded that usage of fruit extract of Meng-kudu can minimization damage of white mouse testis seminiferus tubulus given by cigarette smoke treatmen. But antioksidan with excessive dose can turn into pro-oksidan so that earn damage watering down effect of free radical.Keywords : fruit extract of mengkudu, testis, histopatologi, cigarette smoke
Performa bioflok pada sistem bioflok-akuaponik ramah lingkungan Endah Rita Sulistya Dewi; Maria Ulfah
Bioma : Jurnal Ilmiah Biologi Vol. 11 No. 1: April 2022
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v11i2.10989

Abstract

ABSTRAKBakteri membentuk bioflok, menghasilkan protein mikroba dan memungkinkan untuk mendaur ulang protein pakan yang tidak terpakai. Flok terdiri atas organisme seperti bakteri, plankton, jamur, alga, dan partikel tersuspensi yang mempengaruhi struktur dan nutrisinya. Pembentukan bioflok bertujuan untuk meningkatkan pemanfaatan nutrien, menghindari stres lingkungan dan predasi. Akuaponik merupakan kombinasi sistem akuakultur dan hidroponik yang saling menguntungkan untuk mengatasi masalah kualitas air di lingkungan akuakultur. Sistem bioflok yang digabungkan dengan sistem akuaponik dapat mengurangi pergantian air dalam sistem budidaya sehingga lebih ramah lingkungan. Tujuan penelitian adalah untuk mengetahui performa bioflok pada sistem bioflok akuaponik ramah lingkungan.  Penelitian ini menggunakan tiga perlakuan yaitu P1 (tanpa probiotik/netral), P2 (probiotik EM4) dan P3 (probiotik Sukses Tani). Pengembangan sistem bioflok akuaponik menggunakan model RnD Borg dan Gall. Hasil penelitian menunjukkan bahwa nilai nutrisi bioflok bervariasi dengan nilai protein P0 6,19 %;  P1 2,59%; dan P2 3,46%. Nilai lemak pada masing-masing kelompok perlakuan adalah P0 0,2%; P1 0,97%; danP2 0,43%. Untuk nilai karbohidrat pada masing-masing perlakuan adalah P0 1,46%;  P1 1,98%; dan P2 1,93%. Hasil pengukuran kualitas air dinyatakan layak untuk pengembangan sistem bioflok akuaponik sesuai SNI 7550:2009. Hasil uji homogenitas varians menunjukkan ketiga perlakuan bersifat homogen dan hasil analisis sidik ragam menunjukkan bahwa Fhitung>Ftabel 5% (4,07) dan >Ftabel 1% (7,59). Hal tersebut menunjukkan bahwa pemberian probiotik komersil dapat meningkatkan performa bioflok terutama kualitas air pada sistem bioflok akuaponik ramah lingkungan. Kata kunci: akuaponik; bioflok; performa; ramah lingkungan  ABSTRACTBiofloc performance on environmentally friendly biofloc-aquaponic system. Bacteria form biofloc, produce microbial protein and make it possible to recycle unused feed protein. The floc consists of organisms such as bacteria, plankton, fungi, algae, and suspended particles that affect the structure and nutrients of biofloc. The formation of biofloc aims to increase nutrient utilization, avoid environmental stress, and predation. Aquaponics is a mutually beneficial combination of aquaculture and hydroponic systems to solve water quality problems in aquaculture environments. Biofloc combined with aquaponics systems can reduce water turnover in cultivation so this technology is claimed as environmentally friendly. The purpose of this study was to determine the biofloc performance of environmentally friendly aquaponic-biofloc systems. This research used three groups, P1(without probiotic/neutral), P2 (probiotic EM4) and P3 (probiotic Sukses Tani). The development of the aquaponic-biofloc system using the Borg and Gall RnD models. The results showed that the nutritional values of biofloc were varied with protein are P0 6,19%; P1 2,59%; and P2 3.46%. For fat, there also differences among groups, for P0 0,2%; P1 0,97%; and P2 0;43%. For carbohydrates were P0 1,46;, P1 1,98%; and P2 1.93%. The results of the water quality measurement are declared feasible to development of environmentally friendly aquaponic-biofloc system suitable with SNI 7550:2009. The results of the homogeneity varians test showed that the three treatments are homogen and analysis of variance showed that Fcount > Ftable 5% (4,07) and > Ftable 1% (7,59). It is showed that both commercial probiotic improve performance of biofloc in environmentally friendly aquaponic  biofloc systems, especially for water quality.  Keywords: aquaponics; biofloc; environmentally friendly; performance
Adaptation of Four Strawberry Varieties at a Land Altitude of 900 Meters ASL with Different Shade Nugroho, Ary Susatyo; Sulistya Dewi, Endah Rita; Ulfah, Maria
Bioma : Jurnal Ilmiah Biologi Vol 12, No 2: October 2023
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v12i2.16525

Abstract

Strawberries are herbaceous plants originating from sub-tropical regions. In Indonesia, which is a tropical climate, Strawberries can adapt well to the highlands of more than 1000 meters above sea level. At present, not much is known about strawberry varieties that are able to adapt to the plains below 1000 masl, so it is necessary to do research to find strawberry varieties that are able to adapt to the plains below 1000 masl. This research was conducted on agricultural land at an altitude of 900 masl in the Ngesrepbalong Village area, Limbangan District, Kendal Regency from September to October 2022. The research design used was a Completely Randomized Design with different types of shading. The treatment used is shading in the form of 50% paranet and 70% paranet. Data will be analyzed statistically with ANOVA and descriptive qualitative analysis. The results showed that the four strawberry varieties were able to adapt to an altitude of 900 meters above sea level with 50-70% paranet shade.