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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Perairan (Aquatic Science) Jurnal Farmasi Andalas Jurnal Sains dan Teknologi Farmasi Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology INDONESIAN JOURNAL OF PHARMACY Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JOPS (Journal Of Pharmacy and Science) JURNAL EDUCATION AND DEVELOPMENT JSFK (Jurnal Sains Farmasi & Klinis) JFIOnline Majalah Kedokteran Andalas Jurnal Farmasi Higea Menara Ilmu Jurnal Penelitian Farmasi dan Herbal Journal of Pharmaceutical and Health Research Jurnal Zona REACTOR : Journal of Research On Chemistry And Engineering Pharmaceutical And Biomedical Sciences Journal (PBSJ) Biodiversitas Journal of Biological Diversity
Akmal Djamaan
Fakultas Farmasi Universitas Andalas Padang
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Zam, Syukria Ikhsan; Agustien, Anthoni; Syamsuardi, Syamsuardi; Djamaan, Akmal; Mustafa, Irfan
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Syukria Ikhsan Zam; Anthoni Agustien; Syamsuardi Syamsuardi; Akmal Djamaan; Irfan Mustafa
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells: Cytotoxic Activity of A. nomius NC06 Against HT29 Cancer Cells Artasasta, Muh. Ade; Djamaan, Akmal; Yanwirasti, Yanwirasti; Taher, Muhammad; Djamaludin, Heder; Siswanto, Siswanto; Handayani, Dian
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.05

Abstract

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities.  This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.
The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells: Cytotoxic Activity of A. nomius NC06 Against HT29 Cancer Cells Artasasta, Muh. Ade; Djamaan, Akmal; Yanwirasti, Yanwirasti; Taher, Muhammad; Djamaludin, Heder; Siswanto, Siswanto; Handayani, Dian
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.05

Abstract

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities.  This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.
Co-Authors -, Salman Agusri Boestari Aiyi Asnawi Alviche, Lola Andani Eka Putra Andre Prima Rahmadhana Anthoni Agustien Arfiandi Arfiandi Asia Asia Asiska Permata Dewi Asiska Permata Dewi Asiska Permatadewi Atika Melati Bakhtra, Dwi Dinni Aulia Bilqis Nurganiyu Densi Selpia Sopianti Densi Selpia Sopianti Desma Yuni Dia Indah Azhari Dian Handayani Dian Handayani Dini Assyfa Dini Hanifa Djamaludin, Heder Edtyva Monicha Elmi Sariani Hasibuan Erizal Zaini Fatimah Saidah Gustina &#039; Haliatur Rahma, Haliatur Hari, Dewi Gulyla Ifan Aulia Candra Irfan Mustafa Krisyanella Krisyanella Kustiasih Lestari Laura Syahrul Liswarni, Yenny Lola Alviche Maharani Auliasanrah Siregar Mahrani Napitupulu Majid, Mohammed Isa Abdul Maria Ulfa Marlina . Marlina Marlina Marlina Marlina Marta Aulia Zahra Melati, Atika Melzi Octaviani Mimi Susanti Mohamed Isa Abd. Majid Mohd. Azizan Mohd. Noor Muh Ade Artasasta Muhammad Diki Juliandi Muslim Suardi Musyirna Rahmah Nst Netty Suharti Netty Suharty Noor, Mohd. Azizan Mohd. Novesar Jamarun Nurbailis Purnawan Pontana Putra Putri, Silvy Rizka Rahmi Inayati Refilda Refilda Rendowaty, Agnes Rezki Amelia Rezki Amelia Rina Wahyuni Rini Agustin Rusmana Wijaya Setia Ningrat Rustini Rustini Rustini Rustini S Siswanto Salman - Sarina, Gemmy Shinta, Dewi Yudiana SILVA DIKA RAHAYU Sri Elfina Sri Elfina Syamsuardi Syamsuardi Syukria Ikhsan Zam Taher, Muhammad Witra Aulia Witra Aulia Wiwik Handayani Yanwirasti Yanwirasti Yohannes Alen Yufri Aldi Yulmira Yanti Yutra Noka Zurai Resti