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Phylogenetic Analysis and Mutation of Sars-Cov-2 in Bats in Karst Malang City, Indonesia Keman, Soedjajadi; Moch Irfan Hadi; Dedy Suprayogi; Yudied Agung Mirasa
JURNAL KESEHATAN LINGKUNGAN Vol. 16 No. 2 (2024): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v16i2.2024.173-180

Abstract

Introduction: A group of people in China were hospitalized with an initial diagnosis of pneumonia of unknown cause. The patients were linked to a wholesale wet seafood and animal market in Wuhan, Hubei Province, China. The disease has spread to other provinces in China, Thailand, Japan, and South Korea in less than a month. SARS-CoV-2 was found to originate from bats. Therefore, this research aims to analyze SARS-CoV-2 mutation in bats in Malang Karst, Indonesia. Methods: Other bat body parts used as research samples include the brain, liver, kidneys, intestines, pancreas, fetus, blood, lungs, and ectoparasites. The samples were taken separately and placed in a container containing 10% PBF. For further analysis, we used RNA Extraction, Real-Time PCR, Sequencing, and CoV Gisaid mutation analysis software to analyze the sequencing data. Then, EMBL software will be used to analyze the phylogenetically. Results and Discussion: There was 1 sample that showed a positive result for Covid-19, namely the intestine of the Cynoptera brachyotis species. There were differences between SARS-CoV-2 in bats in Malang Karst in Indonesia compared to SARS-CoV from 2000 to 2019. The spike protein's receptor binding domain (RBD) is the most variable part of the coronavirus genome. Conclusion: From the research results, one positive sample was obtained using Real-Time PCR, and based on mutation analysis, mutations were found in SARS-CoV-2 against the SARS-CoV virus from 2000-2019. Further research is needed, especially regarding SARS-CoV-2 as a vaccine.
Handling Solutions for Electronic Waste (E - Waste) in Indonesia Suprayogi, Dedy
Al-Ard: Jurnal Teknik Lingkungan Vol. 1 No. 2 (2016): March
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v1i2.115

Abstract

Any changes always take effect to the environment either positive or negative, it is no exception developments in technology. In addition to bringing benefits to the human development, technology has always had a negative impact, actually thrash or waste from obsolete technology as the results of the process productions or side product. The mobile phones waste is one of the electronic waste that need to be awareness. Electronic waste processing in particular mobile phones as one of the new problems in the waste management, and it will be an ice mountain problem for developing countries because generally they have limited technology, insufficiency of financial and lack of human resources in the management of electronic waste that electronic trash left to accumulate for granted. Indonesia as a developing country need to strengthening regulatory, economic support and technology transfer of electronic waste management from developed countries to suppress pollution caused by electronic waste disposal carelessly.
Analisis Kualitas Air Sungai Bawah Tanah Gua Ngerong, Kecamatan Rengel, Tuban Suprayogi, Dedy; Nengse, Sulistiya; Hakim, Abdul
Al-Ard: Jurnal Teknik Lingkungan Vol. 5 No. 1 (2019): September
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v5i1.748

Abstract

Fulfillment of water quality requirements is absolutely necessary to fulfill primary human needs, especially drinking water and clean water sources, both in quantity and quality. Study of the underground caverns found the fact that in terms of quantity of water availability in the reservoir is able to meet the needs of residents of the Rengel District, but qualitatively not yet known the water quality in the reservoir. The purpose of this study was to measure the water quality of underground caves in caves which include temperature, turbidity, TDS, DO and pH. The method used in this study is a qualitative method to measure the value of water quality. The research sample was taken with three repetitions for data validity. The sampling location is the underground cave system with a length of 1 km, sample points include dark zone 1, dark zone 2, dark zone 3, and dim zone. Measurements were taken in the laboratory. The results of the study showed that the average values for temperature, turbidity, TDS, DO and pH were 26.7; 1,9; 348.2; 1,6; and 7.1. The conclusion obtained from this study is that all indicators except DOs meet environmental quality standards set by the government.
Fitoremediasi Tanaman Apu-Apu (Pistia Stratiotes) terhadap Kadar Logam Zn Berdasarkan Variasi Jumlah Tanaman Oktaviani, Laili; Nilandita, Widya; Suprayogi, Dedy
Al-Ard: Jurnal Teknik Lingkungan Vol. 6 No. 1 (2020): September
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v6i1.981

Abstract

Zn merupakan logam berat yang banyak digunakan untuk memenuhi kebutuhan industri. Meningkatnya penggunaan Zn menyebabkan pencemaran air. Untuk mereduksi hasil buangan dari industri yang menghasilkan Zn dapat dilakukan dengan cara teknik fitoremediasi. Pada penelitian ini, fitoremdiasi yamg dilakukan yaitu dengan memanfaatkan tanaman Apu-Apu (Pistia stratiotes) sebagai tanaman fitoremediator. Penelitian ini dilakukan dengan tujuan untuk mengetahui efisiensi penyerapan logam berat Zn oleh tanaman Apu-Apu dengan variasi jumlah tanaman yang berbeda. Variasi jumlah tanaman yang digunakan yaitu 10 dan 15 tanaman dengan waktu tinggal selama 10 hari dan dengan konsentrasi awal 11,02 ppm. Hasil penelitian ini menunjukkan bahwa pada reaktor B memiliki efisiensi removal logam berat Zn sebesar 83,85% dan pada reaktor C memiliki efisiensi removal sebesar 79,50%.Kata Kunci: Logam Zn, Fitoremediasi, Pistia stratiotes, Sistem Batch
Analisis Uji Toksisitas Akut Logam Cu Terhadap Artemia salina dan Daphnia magna Suprayogi, Dedy; L, Sri Hidayati; M.Ratodi; Ardilla, Farhana Fitri
Al-Ard: Jurnal Teknik Lingkungan Vol. 7 No. 1 (2021): September
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v7i1.1333

Abstract

Water pollution can become dangerous if consumed. River could be polluted with some materials namely heavy metal of chopper (Cu). Heavy metals that are accidentally consumed by human body through the skin surface, some through the respiratory or digestive tract and then accumulate in the body over time. To make an early detection of heavy metal and water quality in water bodies, some animals or plants were used. Several types of crustaceans such as Artemia salina and Daphnia magna or commonly referred to as bio indicators. This study analyze comparison of acute toxicity test that represented by LC50 value of Cu in Artemia salina and Daphnia magna. From the research result it was found that Artemia salina and Daphnia magna gave a toxic effect with LC50 values of 11.78 ppm and 7.87 ppm, respectively.
Pemetaan Kondisi Sanitasi Lingkungan Dasar Serta Risiko Penyakit Pada Pondok Pesantren Di Kota Surabaya Nilandita, Widya; Diyanah, Khuliyah Candraning; Suprayogi, Dedy
Al-Ard: Jurnal Teknik Lingkungan Vol. 7 No. 2 (2022): March
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v7i2.1452

Abstract

Sanitation is the most important health-related aspect of public health and the environment. Aspects of environmental health in sanitation include community clean water facilities, waste management, waste management, and management of sanitation facilities. Islamic boarding schools are one of the institutions that have problems in sanitation, cases are often found related to students who are infected with several diseases. This study aims to map the sanitation conditions and the risk of disease in Islamic boarding schools in the city of Surabaya. This study uses the observation method for data collection, then analyzed by quantitative description. The sanitation conditions of Islamic boarding schools in Surabaya City from the aspect of clean water are in a good category 55.2%, 41.4% very good, and 3.4% less. Based on the waste aspect, the category result sufficient, less, good, and very good is 52%,  24%, 17%, and 7%, respectively. Furthermore, based on aspects of toilet and bathroom hygiene, the majority was very good, 82.8%, sufficient category was 13.8%, and the good category was 3.4%; and based on wastewater aspects, 100% Islamic boarding school was a very good category. In conclusion, the sanitation conditions of Islamic boarding schools in Surabaya City based on the waste aspect are sufficient; however, based on clean water, the hygiene aspects of toilets and bathrooms, and the aspect of wastewater are very good.
Analisis Kariometric Akibat Pemberian Variasi Dosis Aspartam pada Perkembangan Fetus Mencit (Mus Musculus L.) Esti Novi Andyarini; Nova Lusiana; Ika Mustika; Estri Kusumawati; Sri Hidayati; Moch Irfan Hadi; Risa Purnamasari; Dedy Suprayogi
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.2.86-95

Abstract

Aspartame is safe for consumption according to the prescribed ADI, but this artificial sweetener is not nutritious or does not contain calories so it is not recommended to be consumed during pregnancy. This study was to analyze cariometric effect of aspartame doses in the development of fetal mice (Mus muculus L.). The type of research used was experimental laboratory research on 24 adult mice with 4 treatment groups, control, aspartame dose 13 mg / Kg BB, 39 mg / Kg BB and 78 mg / Kg BB. Data was analyzed using SPSS 16 with one way ANOVA. Based on the results of the study there were changes in the number and weight of the fetus due to aspartame doses, there was no change in fetal length due of aspartame dose variations. Consumption of aspartame during pregnancy should be avoided because it can cause teratogenic effects. For further research, it is necessary to observe the morphology and histology of the fetus which is expressed by aspartame to determine the types of congenital abnormalities.
Quality of cow dung composting fertilizer with additional starter solution of cow rumen contents Amirul Nurdiansyah; Arqowi Pribadi; Dedy Suprayogi; Abdillah Akmal Karami
Konversi Vol 12, No 1 (2023): April 2023
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/k.v12i1.14357

Abstract

RPH Kedurus produces livestock solid waste in the form of cow dung as much as 750 kg/day and the content of cow rumen is 2 tons/day. The livestock solid waste can be processed by turning it into compost using the takakura composting method. This study aims to determine the characteristics of the compost according to the specified parameters (temperature, pH, moisture content, organic c, nitrogen, phosphorus, potassium, and the C/N ratio) and to determine the dose of use of a good cow rumen content solution to produce compost according to the quality standard of SNI 19-7030-2004. In this study, the treatments used were P0 with a composition of 3 kg of cow dung and 1 kg of rice husks, P1 with a composition of P0 and 100 mL of starter solution of cow rumen contents, P2 with a composition of P0 and 200 mL of starter solution of cow rumen contents, and P3 with a composition of P0 and 300 mL of starter solution for bovine rumen contents. Overall, the characteristics produced by the four treatments met the quality standards, while the optimum dosage for the use of rumen contents was found in treatment P2 with the use of 200 mL of starter bovine rumen contents and produced a temperature of 29 °C, pH 7.4, water content 14.2%, C. -organic 67 %, nitrogen 4.26 %, phosphorus P2O5 0.37 %, potassium K2O 2.21 %, and C/N ratio 15.7. The compost fertilizer produced can be used as an alternative organic fertilizer to be developed.
Decoding Sanitation Practices: A Comparative Assessment of Knowledge, Attitudes, and Behaviours Dedy Suprayogi; Utama Teguh Taruna
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 10 No 1 (2026): Medical Technology and Public Health Journal March 2026
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v10i1.7030

Abstract

Adequate sanitation is a crucial factor in improving public health. Knowledge, practice, and behaviors play a key role in the success of sanitation programs. Fundamental aspects of sanitation are the availability of trash bins, which can reduce disease transmission risks. Moreover, in the Lumajang district, the village of Tegalrandu and RanuRanupakis shows an 80% trash bin ownership rate, aligning with national and global standards. However, differences in community knowledge, attitudes, and behaviors between the two villages remain unclear. This study aims to decode sanitation practices by assessing and comparing the knowledge, attitudes, and behaviors of the communities in Tegalrandu dan RanuRanupakis villages regarding environmental sanitation. An analytic study with a cross-sectional design was conducted, involving 80 respondents selected through simple random sampling, with 40 participants from each village. The Mann-Whitney statistical test was employed to analyze differences between the two groups. The results indicated that the significance values for knowledge (0,766), attitude (0,120), and behavior (0,298) were all higher than the α value of 0,05, which suggests no statistically significant differences between the two communities. The findings reveal that despite geographical differences, both villages exhibit similar sanitation knowledge, attitudes, and behaviors. This implies that external factors such as economic conditions, socio-cultural aspects, government policies, and sanitation program interventions may have a greater influence on community sanitation practices. Future efforts should focus on enhancing public awareness and sustainability in sanitation programs to achieve long-term environmental health improvements.
Redesain Instalasi Pengolah Air Limbah Domestik PT A Teguh Taruna Utama; Dedy Suprayogi
Science and Education Journal (SICEDU) Vol 5 No 3 (2026): Science and Education Journal 2026
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/sicedu.v5i3.1358

Abstract

PT A merupakan sebuah perusahaan yang bergerak di bidang penyaluran bahan bakar minyak. PT A berlokasi di Kota Kupang. PT A berupaya untuk memperoleh PROPER dari Kementerian Lingkungan Hidup (KLH). Permasalahan yang dihadapi adalah IPAL eksisting tidak berfungsi sehingga PT A tidak dapat memenuhi syarat untuk mengajukan PROPER. Penelitian ini bertujuan untuk mengetahui permasalahan IPAL, mengevaluasi infrastruktur eksisting dan meredesain IPAL PT A. Metode redesain IPAL akan mengikuti kriteria desain yang sudah ditetapkan oleh KLH. IPAL hasil redesain mengolah air limbah PT A yang mengandung TSS, BOD, COD, dan Total Coliform. Kandungan air sisa proses IPAL berdasarkan hasil redesain, nilai TSS sebesar 25 mg/Liter; BOD sebesar 10 mg/Liter; COD sebesar 75 mg/Liter dan Total Coliform maksimum 200 MPN/100 mL. Air sisa proses IPAL PT A dapat dilepaskan ke drainase atau saluran irigasi. Oleh karena itu, air sisa proses IPAL ini sudah berada di bawah aturan Baku Mutu yang ditetapkan dalam Peraturan Menteri Lingkungan Hidup Nomor 11 Tahun 2025 Baku Mutu Air Limbah dan Standar Teknologi Pengolahan Air Limbah untuk Air Limbah Domestik. Saran dan tindak lanjut bagi PT P adalah membangun IPAL sesuai hasil redesain yang sudah disesuaikan dengan peraturan lingkungan hidup yang berlaku saat ini.