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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Management of Aquatic Resources Journal (Maquares) Journal of Marine Research Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Kelautan Tropis Aquacultura Indonesiana Journal of Tropical Marine Science Journal of Microbial Systematics and Biotechnology Indonesian Journal of Mollusk Journal of Fisheries & Marine

Ali Djunaedi

Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro

Author-ID : 255223

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Library & Information Science

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Study on the Development of Spiral babylonia snail Tiger Snail Juvenil Eggs (Babylonia spirata Linnaeus, 1758) in the Laboratory Muhammad Fatoni; Delianis Pringgenies; Ali Djunaedi
Jurnal Moluska Indonesia Vol. 4 No. 1 (2020): April 2020
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v4i1.22

Several marine gastropods are known to have high economic value, one of which is the Spiral babylonia snail. They commonly exploited for meat, shell, or operculum. Tiger snail meat is commonly exported and consumed by fishermen. Its shell is used as a material for industrial lime and for omamen. Operculum is commonly used as a medicinal and perfume ingredient). However, the recent production has decreased due to the limited information of seasonal catch. One way to overcome these obstacles is through culture activities. The provision of seeds is an important link in the culture system, therefore the study of the spawning process and larval development is an important requirement for the development of larval rearing techniques. This study aims to determine the development process of juvenil eggs and the survival rate of tiger snails reared in the laboratory.. The study was conducted at the Laboratory of Marine Farm Subdivision in Kudus, Pt Pura Bahari, Jepara Regency. This sudy used experimental method with descriptive data analysis. Adult tiger snails were caught from Bunga waters, Demak. The snails were reared in a rectangular tube filled with 15 cm filtered sea water and 5 cm of sand. Egg capsules were reared in a 4 liters circular tube with continuous aeration. The hatched larvae were reared in a 300 liters circular tube filled with filtered seawater, continuously aerated and fed with Chlorella plankton . Our result showed that tiger snails spawn naturally and produce egg capsules. The incubation period for the capsule is 6 days, then develop into veliger larva for 12 days. The veliger larvae metamorphosed,and become juvenil. The lowest survival rate observed in the veliger stage. The survival rate of the egg and the intracapsular trochopore stages were quite high (tube I = 98.413%; tube II = 98.238%; tube III = 90.476%), then the lowest was in the veliger stage (tube I = 3,468%; tube II = 2.603%; tube III = 2,598%) and the juvenil stage is quite high compared to the previous phase (tube I = 3,413%; tube II = 2,479%; tube III = 2,351%).

Potensi Ekstrak Buah Mangrove Xylocarpus granatum Untuk Pemberantasan Larva Nyamuk Aedes aegypti Delianis Pringgenies; Rini Widiyadmi; Ervia Yudiati; Muhammad Syaifudien Bahry; Ali Djunaedi
Journal of Tropical Marine Science Vol 1 No 1 (2018): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

ABSTRAK Tujuan penelitian adalah untuk mengetahui potensi ekstrak buah mangrove Xylocarpus granatum sebagai anti larva nyamuk Aedes aegypti. Uji larva nyamuk dengan memasukkan masing-masing 10 ekor larva nyamuk yang dimasukkan ke dalam 7 botol cup tranparan kecil, masing– masing untuk 50, 100, 250, 500, 1000 ppm sampel ektrak, kontrol negative dan kontrol positive. Hasil penelitian memperlihatkan bahwa pengaruh tingkat mortalitas larva nyamuk terhadap ekstrak buah mangrove Xylocarpus granatum pada jam 1 memperlihatkan bahwa pada konsentrasi 1000 ppm, persentasi mortalitas nyamuk tertinggi adalah 81,34 %, dan pada konstrasi ekstrak buah mangrove Xylocarpus granatum 100 ppm terendah mortalitasnya = 68,8%. Perlakuan pada jam 3 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove 500 ppm tingkat mortalitas larva nyamuk tertinggi (80%). Pada konsentrasi ekstrasi buah mangrove 250 ppm, mortalitasnya 66,70% dan konsentrasi ekstral buah mangrove pada 100 ppm, mortalitasnya adalah 40%. Hasil peneltian pada jam ke 24 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove mencapai 1000 pmm, mortalitasnya 100%, pengamatan jam ke 48 mortalitasnya 100%. Sedang pada perlakuan control positif, keluulushidupan larva nyamuk adalah 0% dan pada control negative, kelulushidupan larva nyamuk adalah 100%. Kesimpulan: bahwa ekstrak buah mangrove Xylocarpus granatum berpotensi sebagai anti larva nyamuk pada konsentrasi 1000 ppm. ABSTRACT It was assumed that mangrove fruits has some insecticidal biosubstances. Aim of the research is the potentials of mangrove Xylocarpus granatum fruit extract as the anti mosquito (Aedes aegypti) repellent. Experiment using of 10 mosquito larvae in 7 bottles each filled with 50; 100; 250; 500 and 1000 ppm fruit extract, negative and positive control. The experiment reveals that after 1 hour treatment, the 1000 ppm exctract had the hihgest mosquito larvae mortality of 81.34%, while the lowest extract of 100 ppm had 68.8% mortality. After 3 hours of extract treatment the 500 ppm had the hihgest mortality of 80%, 250 ppm with 66.70% and 100 ppm with 40 % mortality. Result of experiment after 24 as well as 48 hours treatment the 1000 ppm extract had 100% mortality. In the positive control had 0% mortality and the negative control had 100% of survival. The summary is that the mangrove Xylocarpus granatum fruit extract had a potential as mosquito repellent at 1000 ppm fruit extract.

Analisis Aspek Reproduksi Kepiting Bakau (Scylla sp.) Di Perairan Kendal, Jawa Tengah Ester Tiurlan; Ali Djunaedi; Endang Supriyantini
Journal of Tropical Marine Science Vol 2 No 1 (2019): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Kepiting bakau Scylla sp. merupakan salah satu biota laut yang mempunyai nilai ekonomis penting. Penyebarannya hampir di seluruh kawasan pesisir Indonesia, yang memiliki ekosistem mangrove termasuk di perairan Kendal. Banyaknya penangkapan kepiting bakau tanpa memperhatikan ukuran yang layak tangkap. Oleh karena itu penelitian ini dilakukan untuk memperoleh beberapa aspek reproduksi kepiting bakau Scylla sp. di perairan Kendal, Jawa Tengah. Pengambilan sampel dilakukan pada bulan Februari–Maret 2017, dua minggu sekali selama dua bulan di perairan Kendal, Jawa Tengah. Metode yang digunakan dalam penelitian ini adalah metode deskriptif eksploratif dengan parameter pendukung seperti suhu, salinitas, pH, dan DO. Materi yang digunakan adalah kepiting bakau Scylla sp. Analisis yang digunakan untuk menghitung perbandingan jumlah kepiting bakau jantan dan betina adalah uji chi square. Hasil penelitian menunjukkan bahwa komposisi ukuran kepiting bakau mempunyai lebar karapas 81-130 mm, berat 100-350 gram dengan ukuran rata-rata yang tertangkap 99,62 mm, perbandingan jantan dan betinanya 1:2,09. Hubungan lebar karapas dengan berat menunjukkan sifat allometrik positif. Faktor kondisi yang didapatkan adalah 1,0185. Tingkat kematangan gonad kepiting bakau di dominasi oleh TKG II, nilai indeks kematangan gonad pada kepiting bakau betina berkisar antara 6,84% - 18,49%. Tingkat kematangan gonad yang di dominasi oleh TKG II dikarenakan di lokasi penelitian tidak sedang masa pemijahan. Mangrove crab (Scylla sp.) is one of the marine biota that it has value economically important. It spreads in almost all over coastal areas of Indonesia, which has the mangrove ecosystem. Mangrove crab has a high nutritional value. Thus, encouraging people in Kendal to consume a large amount of mangrove crab and reach the local market demand.This led to the occurrence of a lot of mangrove crab catching regardless of the size of the catch.Therefore, this study was conducted to obtain some of reproduction aspects of Scylla sp. in the Kendal waters, Central Java. Sampling was conducted in February-March 2017,every two weeks in two months in Kendal waters, Central Java. The method used in this research was descriptive explorative method with supporting parameters such as temperature, salinity, pH, and DO. The used material was mangrove crab (Scylla sp.). Chi square test was used to analyze and calculate the ratio of male and female mangrove crabs. The results showed that the size composition of mangrove crab had a carbide width of 71-130 mm, weight of 100-350 grams, with average size captured was 99.62 mm, male and female ratio of 1: 2.09. The Relations between width and weight of caparace indicated negative allometric properties. The obtained condition factor was 1.0184767. Maturity level of mangrove crab gonad was dominated by TKG II, gonad index maturity value on female mangrove crab ranged between 6.84% - 18.49%. The maturity level of gonad was dominated by TKG IIbecause the research location was not in the spawning period.

Stability of Phycocyanin Extracted from Spirulina maxima in different pH from Indoor and Semi-outdoor Cultivation Dwi Susilaningsih; Bimo Saskiaoktavian; Ali Djunaedi; Agus Trianto
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.33

Spirulina maxima is a blue-green microalga that rich in pigment. The pigments in S. maxima grouped into primary pigment (Chlorophyll a) and accessory pigments (carotenoid & phycobiliprotein). Phycocyanin is an accessory pigment that belongs to phycobiliprotein, blue colored, and can be used as natural food coloring and drugs. Phycocyanin has activities as antibacterial, antioxidant, anti-inflammatory, antihyperalgesic, and many more. Because of that, phycocyanin usually used in the pharmaceutical industry. However, phycocyanin is a protein that unstable under lights, high temperature, and pH in the storage. This study aims to obtain information about the effect of pH on the stability of phycocyanin extracted from S. maxima that cultivated in indoor and semi-outdoor. The steps are cultivation, extraction using different solutions to get blue pigment phycocyanin, and stability test. Phycocyanin was dissolved in a buffer solution at pH 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, and 7.0 for 7 days. Color observations based on visualization and concentration measurements are carried out every day to see changes in phycocyanin. Growth in indoor cultivation with 24 hours light is faster than semi-outdoor a that uses only sunlight. Cultivation gets the optical density value 0,6 at day 20 with indoor cultivation and day 34 with semi-outdoor cultivation. Results show that phycocyanin can be extracted using a buffer phosphate solution. The stability of the pigments can be seen from the color changes and relative concentration using a spectrophotometer. Phycocyanin shows stable in the storage of pH 4 until pH 5.5. The highest relative concentration (CR) was shown in pH 5.

Simpanan Karbon Enhalus acoroides LF. Royle 1839 (Angiosperms: Hydrocharitaceae) di Pantai Gelaman dan Pantai Alang-Alang, Karimunjawa Jepara Viny Ratnasari; Ali Djunaedi; Adi Santoso
Journal of Marine Research Vol 9, No 1 (2020): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

ABSTRAK: Perubahan iklim disebabkan oleh berbagai aktifitas kegiatan manusia yang menghasilkan gas karbon diokasida ke atmosfer bumi yang akan berdampak pada pemanasan global. Ekosistem padang lamun memiliki kemampuan untuk menyerap dan menyimpan karbon dalam jumlah besar dari atmosfer yang dapat mengurangi emisi karbon. Tujuan penelitian ini untuk mengetahui kerapatan, tutupan lamun, biomassa dan simpanan karbon pada lamun Enhalus acoroides di Pantai Gelaman dan Pantai Alang-Alang. Penelitian menggunakan metode survei dan penentuan lokasi dipilih dengan menggunakan metode line transect quadrant yang mengacu pada metode LIPI. Sampling dilakukan pada titik 50 m setiap substasiun dengan metode pencuplikan. Pengukuran karbon pada sampel lamun menggunakan metode LOI.Kerapatan lamun di Stasiun 1 sebesar 1235 ind/m2 dan nilai tutupan lamun sebesar 68,76%. Kerapatan Stasiun 2 sebesar 1135 ind/m2 dan tutupan lamun sebesar 51,78%. Nilai rata-rata estimasi simpanan karbon lamun Enhalus acoroides di Stasiun 1sebesar 119.27 gC/m2 dan di Stasiun 2 sebesar 91.57 gC/m2. ABSTRACT: Climate change is caused by various human activities that produce carbon dioxide gas into the earth atmosphere which will have an impact on global warming. Seagrass ecosystem is able to absorb and store large number of carbon from the atmosphere that can reduce carbon emissions. This research were to determine the density, seagrass cover, biomass and carbon storage in seagrasses at Gelaman Beach (Station 1) and Alang-Alang Beach (Station 2). Survey and sampling. Were conducted using quadrant transect referring to LIPI method. Observation of the density value, the percentage of seagrass coverage was conducted in all points, while the sampling was conducted at the point 50 m on each substation by sampling method. Carbon measurement in seagrass sample used LOI method. Total seagrass density in Station 1 was 1235 ind/m2 and the total value of seagrass percentage cover was 68,76%. Total seagrass density in Station 2 was 1135 ind/m2 and total value of seagrass percentage cover was 51,78%. Average value for carbon savings estimated seagrass Enhalus acoroides in Station 1 was 119.27 gC/m2 and Station 2 was 91.57 gC/m2.

Perbedaan Lama Fotoperiode Terhadap Total Lipid Kultur Mikroalga Chlorella vulgaris Hendrik Surya Bahar; Ali Djunaedi; Widianingsih Widianingsih
Journal of Marine Research Vol 11, No 1 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i1.32211

Chlorella vulgaris merupakan salah satu mikroalga yang mengandung lipid, oleh karena itu mikroalga C. vulgaris dapat dimanfaatkan sebagai sumber pangan fungsional dalam meningkatkan kesehatan. Pertumbuhan mikroalga dipengaruhi oleh berbagai macam faktor lingkungan salah satunya adalah cahaya. Penelitian ini bertujuan untuk mengetahui fotoperiode terbaik guna meningkatkan pertumbuhan dan kandungan total lipid pada C. vulgaris. Metode yang digunakan adalah eksperimen laboratoris. Mikroalga C. vulgaris dikultivasi dengan 4 perlakuan siklus fotoperiode (terang:gelap) yang berbeda yaitu A (4 : 20), B (8 : 16), C (12 : 12) dan D ( 24 : 0). Penelitian ini menggunakan pupuk walne. Suhu air pada penelitian ini berkisar antara 18-21°C. Salinitas berkisar antara 29-32 ppt, kisaran pH pada penelitian ini antara 7,3-8,0. Pertumbuhan C. vulgaris diamati selama 9 hari kemudian dipanen untuk perhitungan biomassanya. Biomassa basah hasil kultivasi dikeringkan dan dilakukan uji kadar air. Total lipid ditentukan secara gravimetrik. Biomassa disoxhletasi menggunakan n-heksan. Fraksi lipid dan n-heksan dipisahkan dengan distilator, berat ekstraknya terhitung sebagai kandungan total lipid. Hasil penelitian menunjukkan bahwa perlakuan fotoperiode berpengaruh secara nyata (p<0,05) terhadap kepadatan sel mikroalga dan kandungan total lipidnya C. vulgaris. Perlakuan D memiliki hasil kepadatan tertinggi sebesar 1.131x104 sel/mL. Perlakuan C memiliki hasil kandungan total lipid tertinggi dengan total 17%. Perlakuan A memperoleh hasil terendah dengan kepadatan sebesar 638x104 (sel/mL) dan kandungan total lipid 8,7%.Chlorella vulgaris is one of the microalgae containing lipids, therefore the microalgae C. vulgaris can be used as a source of functional food to improve health. Microalgae growth is influenced by various environmental factors, one of which is light. This study aims to determine the best photoperiod to increase growth and total lipid content of C. vulgaris. The method used is a laboratory experiment. Microalgae C. vulgaris was cultivated with 4 different photoperiod (light:dark) cycle treatments, namely A (4 : 20), B (8 : 16), C (12 : 12) and D (24 : 0). This research uses walne fertilizer. During the study, water quality measurements were carried out. The water temperature in this study ranged from 18-21°C. Salinity ranged from 29-32 ppt, the pH range in this study was between 7.3-8.0. The growth of C. vulgaris was observed for 9 days and then harvested for biomass calculation. The wet biomass from the cultivation was dried and the moisture content was tested. Total lipid was determined gravimetrically. Biomass was disoxhletated using n-hexane. The lipid fraction and n-hexane were separated by distillation, the weight of the extract was calculated as the total lipid content. The results showed that the photoperiod treatment had a significant (p<0.05) effect on the cell density of C. vulgaris microalgae and also significantly (p<0.05) on the total lipid content. Treatment D had the highest density of 1,131 x 104 cells/mL. Treatment C had the highest total lipid content with a total of 17%. Treatment A obtained the lowest yield with a density of 638 x 104 (cells/mL) and a total lipid content of 8.7%.

Alginate from Sargassum sp. Improve the Hematology Performance and Oxygen Tolerance Exposure of Lates calcarifer Ervia Yudiati; Ali Djunaedi; Nuril Azhar; Rabia O. Alghazeer
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.16217

Indonesia's fish production is abundant, especially in aquaculture. Lates calcarifer is farmed fish species. The failure of L. calcarifer cultivation due to disease problems. We are utilizing a natural compound derived from tropical Sargassum sp. extracts, namely alginate. This study aims to determine and analyse the supplementation of alginate in the diet by oral administration to improve the fish's hematological performance and oxygen stress tolerance. There were one control and three treatments (2 g.kg-1, 4 g.kg-1, and 8 g.kg-1 alginate/feed). The experiment was a completely randomized design with three replications. Fish were reared in a 350 L fiber tank for 12 days at a density of 20 ind.tank-1. Stress oxygen tolerance was applied by rearing the 10 fish in 12 L fully plastic-wrapped containers. The parameters test analyzed were phagocytic activity and phagocytic index, red blood parameters which are hematocrit and hemoglobin. The survival rate after 5 hr anoxic exposure was also recorded. The best treatments were achieved at a dose of 6 g.kg-1 and 8 g.kg-1in all parameters, except the phagocytic index. The alginate addition at all oxygen stress treatments also performed a better survival rate compared to the control. Adding alginate to feed as feed supplementation by oral administration can boost hematological performance and higher tolerance from oxygen stress exposure.

Kandungan Nitrat (NO3-) dan Fosfat (PO4-3) pada Sedimen terhadap Kondisi Penutupan Lamun di Pantai Prawean Jepara Alia Fatimah Azzahra; Munasik Munasik; Ali Djunaedi
Journal of Marine Research Vol 11, No 4 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i4.33924

Lamun dapat menyerap nutrien dari substrat melalui sistem akar yang berperan dalam faktor pertumbuhan lamun tersebut sehingga efisiensi daur nutrien dalam sistemnya menjadi sangat penting untuk memelihara produktivitas primer padang lamun. Nitrat dan fosfat berfungsi sebagai nutrien untuk membantu proses fotosintesis. Selain masukan dari aktivitas daratan, nitrat dan fosfat berasal dari hasil dekomposisi tumbuhan lamun yang telah mati. Pantai Prawean Jepara termasuk kawasan yang didominasi oleh kegiatan wisata, dengan dermaga untuk kapal pesiar selain itu juga termasuk kawasan padat penduduk dengan berbagai aktivitas yang memberikan kontribusi terhadap kontribusi zat organik dan anorganik terhadap perairan sekitarnya sehingga mempengaruhi kadar nitrat dan fosfat di perairan. Penelitian ini bertujuan untuk mengetahui kandungan nutrien (nitrat dan fosfat) pada sedimen serta mengetahui jenis dan tutupan lamun yang terdapat di perairan Pantai Prawean Jepara. Metode penelitian ini yaitu deskriptif eksploratif, sedangkan metode penentuan lokasi menggunakan metode purposive sampling pada tiga stasiun. Penelitian dilaksanakan pada bulan November 2021. Analisis sampel penelitian dilakukan di Laboratorium Pengujian Teknik Lingkungan, Fakultas Teknik, Universitas Diponegoro. Hasil kandungan nitrat dan fosfat dalam sedimen di perairan Pantai Prawean, Jepara pada stasiun 1 hingga 3 memiliki rata-rata nitrat 1,44 – 17,597 ppm tergolong tinggi dan fosfat 0,011 – 0,029 ppm tergolong rendah. Jenis lamun yang ditemukan pada lokasi penelitian sebanyak 4 spesies yaitu Oceana serullata, Cymodocea rotundata, Enhalus accoroides, dan Thalassia hemprichii dengan rata-rata tutupan lamun sebesar 41,41% yang tergolong sedang. Analisis regresi korelasi menunjukan hubungan yang sedang positif pada nitrat yaitu r = 0,34 dan lemah negatif pada fosfat yaitu r = -0,1. Besarnya pengaruh nitrat dan fosfat pada sedimen terhadap kondisi penutupan lamun menghasilkan nilai Determinansi Regresi (R) sebesar 0,4262 (42,62%) dan 0,0368 (3,68%). Seagrass can absorb nutrients from the substrate through the root system which plays a role in the growth factors of the seagrass so that the efficiency of the nutrient cycle in the system is very important to maintain the primary productivity of seagrass beds. Nitrates and phosphates function as nutrients to help the process of photosynthesis. In addition to input from land activities, nitrate and phosphate are derived from the decomposition of dead seagrass plants. Prawean Beach Jepara is an area that is dominated by tourism activities, with a dock for cruise ships, besides that it is also a densely populated area with various activities that contribute to the contribution of organic and inorganic substances to the surrounding waters, thereby affecting nitrate and phosphate levels in the waters. This study aims to determine the nutrient content (nitrate and phosphate) in the sediment and to determine the type and cover of seagrass found in the waters of Prawean Beach, Jepara. This research method is descriptive exploratory, while the method of determining the location using purposive sampling method for three stations. The research was carried out in November 2021. Analysis of the research sample was carried out at the Environmental Engineering Testing Laboratory, Faculty of Engineering, Diponegoro University. The results of the content of nitrate and phosphate in sediments in the waters of Prawean Beach, Jepara at stations 1 to 3 have an average nitrate of 1.44 - 17.597 ppm which is high and phosphate of 0.011 - 0.029 ppm is low. There were 4 species of seagrass found at the study site, namely Oceana serillata, Cymodocea rotundata, Enhalus accoroides, and Thalassia hemprichii with an average seagrass cover of 41.41% which was classified as moderate. Correlation regression analysis showed that the relationship was positive for nitrate, namely r = 0.34 and weakly negative for phosphate, namely r = -0.1. The magnitude of the effect of nitrate and phosphate on sediments on seagrass cover conditions resulted in the Regression Determination (R) values of 0.4262 (42.62%) and 0.0368 (3.68%).

Exploration of Antimicrobial Potency of Mangrove Symbiont Against Multi-Drug Resistant Bacteria Delianis Pringgenies; Wilis Ari Setyati; Ali Djunaedi; Rini Pramesti; Siti Rudiyanti; Dafit Ariyanto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 2 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i2.26199

Highlight ResearchAntimicrobial potential against the test microbesRhizhopora mucronata isolate showed 95% homology with Bacillus subtilis, and 97% homology with Bacillus oceanisediminis,Acanthus ilicifolius isolate showed 96% homology with Paracoccus caeni, and 89% homology with Bacillus circulans. The study found 4 isolates with antimicrobial potency against MDR pathogenic microbes.The symbiont microbes taken from Rhizophora mucronata and Acanthus ilicifolius were determined to be of the genus Bacillus and Paracoccus AbstractAntimicrobial property of mangrove symbiont have the ability to fight Multi Drug Resistant bacteria which were Staphylococcus aureus, Escherichia coli, and Vibrio haryeyi. This study aimed to determine the potential of symbiont microbes from the root of Rhizopora mucronata and Acanthus iilicifolius as antimicrobial agents against multi-drug resistant (MDR) pathogenic microbes. This research was conducted during July to November 2020. The MDR bacteria were S. aureus, E. coli, and V. harveyi MDR test microbes. The symbiont microbes were identified through molecular analyses (PCR 16S rDNA). Isolation of symbiont microbes from R. mucronata resulted in 16 isolates, while isolation from A. iilicifolius resulted in 14 isolates. Based on the antimicrobial qualitative test against S. aureus, 8 out of 16 microbial isolates from R. mucronata were found to show antimicrobial properties. The testing of A. ilicifolius symbiont microbes against S. aureus showed 8 out of 14 isolates with antimicrobial properties. The test against E. coli resulted in 2 out of 16 microbial isolates from R. mucronata and 5 out of 14 isolates from A. ilicifolius with antimicrobial properties. The test against V. harveyi resulted in two out of 16 microbial isolates from R.mucronata and 4 out of 14 isolates from A. ilicifolius with antimicrobial properties. The quantitative test found 2 isolates from R. mucronta, namely isolates RM10 and RM12, with antimicrobial properties against MDR strain E. coli, with the best isolate being RM10, which produced 11.22 mm of inhibition zone diameter. Furthermore, the selection of isolates was based on the size of the inhibition zone, the clearness of the inhibition zone and the potential for antibacterial activity. Based on their overall antimicrobial potential against the test microbes, four isolates were selected. Molecular analyses of RM12 isolate showed 95% homology with Bacillus subtilis, of RM 10 isolate showed 97% homology with Bacillus oceanisediminis, of AC isolate showed 96% homology with Paracoccus caeni, and of AC 5 isolate showed 89% homology with Bacillus circulans. The study found four isolates with antimicrobial potency against MDR pathogenic microbes. The symbiont microbes taken from R. mucronata and A. ilicifolius were determined to be of the genus Bacillus and Paracoccus.

Estimasi Simpanan Karbon dan Bioekologi Lamun di Pantai Prawean, Jepara Endarwantti, Vionita; Djunaedi, Ali; Santosa, Gunawan Widi
Journal of Marine Research Vol 12, No 4 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i4.35699

Perubahan iklim global yang semakin meningkat dapat menyebabkan kenaikan kadar karbondioksida pada atmosfer. Lamun dapat mengurangi kadar karbondioksida pada atmosfer dengan cara menyerap karbon anorganik melalui proses fotosintesis lamun dan menyimpannya dalam bentuk karbon organik dalam jangka waktu lama. Oleh karena itu penelitian ini bertujuan untuk mengetahui komposisi jenis, kerapatan, penutupan, indeks ekologi, biomassa, dan karbon pada vegetasi lamun di Pantai Prawean, Jepara. Penelitian ini menggunakan metode pengambilan data secara purposive sampling. Pendataan kondisi padang lamun mengacu pada metode LIPI 2014 dengan mendata penutupan, dan kerapatan lamun. Pengambilan sampel lamun menggunakan seagrass core secara acak. Sampel lamun diolah dengan metode Loss On Ignition (LOI) untuk mengetahui kadar karbon. Selanjutnya data kadar karbon dikonversikan berdasarkan nilai biomassa lamun. Jenis lamun yang ditemukan di Pantai Prawean berjumlah 5 jenis antara lain Cymodocea rotundata, Thalassia hemprichiii, Oceana serrulata, Enhalus acoroides dan Halodule uninervis. Kerapatan lamun di Pantai Prawean dapat mencapai 1717 individu/m2 pada stasiun 1 dan 780 individu/m2pada stasiun 2. Persentase penutupan lamun total di Pantai Prawean sebesar 39,20 % pada stasiun 1 dan 22,73 % pada stasiun 2. Indeks ekologi pada stasiun 1 memiliki keanekaragaman rendah, keseragaman rendah dan ada dominasi lamun. Sedangkan pada stasiun 2 memiliki keanekaragaman sedang, keseragaman sedang dan dominasi sedang. Total biomassa lamun pada stasiun 1 dan stasiun 2 sebesar 2970,01 g/m2 dan 1345,85 g/m2. Total estimasi kandungan karbon lamun pada stasiun 1 dan stasiun 2 sebesar 1000,61 gC/m2 dan 447,27 gC/m2. Global climate change can cause an increase in carbon dioxide levels in the atmosphere. Seagrass can reduce carbon dioxide levels in the atmosphere by absorbing inorganic carbon through the seagrass photosynthesis process and storing it in the form of organic carbon for a long time. Therefore, this study was conducted to determine species composition, density, cover, ecological index, biomass, and carbon in seagrass vegetation in Prawean Beach, Jepara. This research uses purposive sampling data collection method. Data collection on the condition of seagrass beds refers to the 2014 LIPI method by recording the cover and density of seagrass. Seagrass sampling used random seagrass cores. Seagrass samples were processed by the Loss On Ignition (LOI) method to determine the carbon content. Furthermore, the carbon content data is converted based on the value of seagrass biomass. There are 5 types of seagrass found on Prawean Beach, namely Cymodocea rotundata, Thalassia hemprichiii, Oceana serrulata, Enhalus acoroides and Halodule uninervis. The density of seagrass in Prawean Beach can reach 1717 individuals/m2 at station 1 and 780 individuals/m2 at station 2. The percentage of total seagrass cover in Prawean Beach is 39.20% at station 1 and 22.73% at station 2. station 1 has low diversity, low uniformity and there is a dominance of seagrass. While at station 2 has moderate diversity, moderate uniformity and moderate dominance. The total biomass of seagrass at station 1 and station 2 was 2970.01 g/m2 and 1345.85 g/m2. The total estimated carbon content of seagrass at station 1 and station 2 is 1000.61 gC/m2 and 447.27 gC/m2.

Co-Authors A. Santoso Adi Santosa ADI SANTOSO Adi Santoso Adi Suryanto Adi Suryanto Agung Sudaryono Agus Sabdono Agus Trianto Ali Ridlo Alia Fatimah Azzahra Ambariyanto Ambariyanto Amelia, Aninda Putri Anggelina, Amelia Cahya Anggit Puji Muswantoro Anggit Puji Muswantoro Aninda Putri Amelia Apriliani, Seka Indah Ardi Ristiyanto Ayu Permana Sari Ayu Permana Sari Ayunda Ainun Nisa Ayuning Smita Rukmi Ayuning Smita Rukmi Bagus Pitra Aditya Bagus Pitra Aditya Bagus Pitra Aditya Bambang Sulardiono Bambang Yulianto Baskoro Rochaddi Bimo Saskiaoktavian Bintang Septiarani Br Ginting, Feny Amelia Chrisna Adhi Suryono Chrisna Adi Suryono Cinthya Ruhanto Putri Dafit Ariyanto Dea Shinta Kharisma Adziana Delianis Pringgenies Delianis Pringgenies Dwi Susilaningsih Dwicahyo Setiyo Wibowo Dyah Rahmawati Endang Supriyantini Endarwantti, Vionita Ervia Yudiati Ervia Yudiati Ester Tiurlan Farizan Adiya Pratama, Farizan Adiya Fauzi Anwar Fauzi Anwar Fauzia Farida Feny Amelia Br Ginting Gunawan Widi Santosa Hargo Seno Wahyu Edi Haryo Farras Raditya Hutama Hendrik Surya Bahar Heri Susilo Humairah Arifia Sabiladiyni I Nyoman Widiasa Ibnu Pratikto Ichsan Suryo Wibowo Irwani Irwani Ita Riniatsih Ivend Umbu Jawa Ivend Umbu Jawa, Ivend Umbu Jakfar Shodiq Panatar Jauharul Fadli Kharisma Firdaus Linggarjati Kharisma Firdaus Linggarjati Lestari, Raisha Fahmida Dwi Mega Fatimah Rosana Muhammad Eka Darmawan Rafsanjani Muhammad Fatoni Muhammad Syaifudien Bahry Niâ€™amillah Niâ€™amillah Ni’amillah Ni’amillah Norma Afiati Nur Taufiq Nur Taufiq S.P.J. Nuril Azhar Panatar, Jakfar Shodiq Petrus Subardjo Priyo Sasmoko Putri, Cinthya Ruhanto Rabia Alghazeer Rabia O. Alghazeer Raden Ario Radila Widya Shafiya Rafsanjani, Muhammad Eka Darmawan Raisha Fahmida Dwi Lestari Retno Hartati Retno Hartati Retno W. Astuti Reza Hafiz Pranajaya Ria Azizah Ria Azizah Ria Azizah Tri Nuraini Richardus Mahatmada Indrajati Rini Pramesti Rini Widiyadmi Ristiyanto, Ardi Rizqina, Cahya Rudhi Pribadi Sabiladiyni, Humairah Arifia Safitri, Shofi Firda Sarah Pebriyani Turnip Sardjito Sardjito Sari Budi Moria Sarjito - Sarjito . Seka Indah Apriliani Setyati , Wilis Ari Shafiya, Radila Widya Shofi Firda Safitri Siti Aminah Siti Aminah Siti Rudiyanti Sri Redjeki Sri Redjeki Subagiyo Subagiyo Subagiyo Subagiyo Subagyo Subagyo Sunaryo Sunaryo Sunaryo Sunaryo Sunaryo Sunaryo Sunaryo Sunaryo Suryono Suryono Tri Adi Nugroho Turnip, Sarah Pebriyani Ulfah Rahmayani Viny Ratnasari W. Widiatmoko Wahyu Febrianto Wibowo, Ichsan Suryo Widianingsih Widianingsih Widianingsih Widianingsih Wilis Ari Setyati Zaenal Arifin

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