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Effect of Temperature, Storage Time, The Residual Test of Neem Oil Formulation (Azadirachta indica A. JUSS) and Bitung Formulation (Baringtonia asiatica) to Its Toxicity Against Large Cabbage Heart Caterpillar (Crocidolomia pavonana F) Neneng Sri Widayani; Afifah Nashirotul Haq; Lindung Tri Puspasari; Yusup Hidayat; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.433 KB) | DOI: 10.24198/cs.v1i1.16996

Abstract

Effectiveness of pesticide affected by many factors such as physical environment and duration of storage. This study aims to determine the effect of temperature and storage time of formulation of neem 50 EC and residual effect of formulation of neem 50 EC and formulation of bitung 30 SC against C. pavonana larvae. The formulations tested on the experiment of effect storage time and temperature test using the newly prepared, neem formulation has been stored for 19 months at room temperature 24.6-31.7oC, and stored in low temperature (4oC) as well as controls. The toxicity of the neem oil formulation was tested at concentrations of 1%, 2%, and 3%. The experiment of residual effect was performed by spraying of the formulation on broccoli leaf, then the leaf samples were taken on days 0,1,2,3,5,7,9,11, and 13 days after application of insecticide. The results of the research indicated that storage at low temperatures (temperature 4oC) for 19 months can maintain the toxicity of the neem formulation to C. pavonana larvae. while storage at room temperature for 19 months resulted in a decreased toxicity. The residual activity of the neem formulations at concentration of 0.8% and 2.4% were not caused mortality of C. pavonana larvae at the residual age of 13.34 days and 14.66 days. The activity of Bitung formulation at concentration of 1.1% and 2.8% were not caused mortality of C. pavonana larvae at the residual age of 11.33 days and 16.26 days.
Toxicity of Methanolic Seed Extract of Barringtonia asiatica L. (Kurz) (Lecythidaceae) from Different Place in Indonesia Against Spodotera litura F (Lepidoptera: Noctuidae) Danar Dono; Irpan Permana
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.709 KB) | DOI: 10.24198/cs.v1i2.19754

Abstract

One of the potential plants to be developed as insecticide is Barringtonia asiatica (Lecythidaceae). Phytochemicals of the same species plant is different in different place and time. This research was conducted to determine toxicity methanolic seed extract of Barringtonia asiatica from different field in Java i.e. Pangandaran, Sindangkerta, Bandung, Jatinangor, and Yogyakarta against Spodoptera litura. The evaluation of toxicity was carried out using feeding method. The result indicated that seed extract of B. asiatica showed value of toxicity from higher to lower were extract of B.asiatica from Pangandaran, Jatinangor, Sindangkerta, Bandung, and Yogyakarta respectively. The extract of B. asiatica showed rendement from higer to lower were extract of B.asiatica from Pangandaran (37.8%), Sindangkerta (36.1%), Yogyakarta (29.3%), Bandung (26.5%) and Jatinagor (25.8%). Methanolic seed extract of B.asiatica from Pangandaran showed the highest toxicity and rendement compared from the other place, with LC at 0.53%. In addition of toxic property, the extract of B. asiatica seed decreased of 50 leaf consumption and pupal weight and lenghtened development time of tested larvae. Keywords:  Biology, extract, toxicity, Barringtonia asiatica, Spodoptera  litura
Susceptibility of Brown Planthopper (Nilaparvata lugens Stal.) from Cipunagara and Banyuwangi Population to Neem Oil Formulation Neneng Sri Widayani; Danar Dono; Yusup Hidayat
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.675 KB) | DOI: 10.24198/cs.v1i2.20331

Abstract

Brown Planthopper is one of the pests that cause problems in rice production. This study was conducted to know the tendency of using a kind of insecticide and to examined the susceptibility of brown planthopper from field population (Cipunagara, West Java) compared to standard population (Banyuwangi, East Java) to formulation insecticide of neem oil 50 EC. Observations wereconducted on Cipunagara farmers to find out the kind of insecticide used and the number of insecticide applications in one season. the susceptibility test to neem oil 50 EC formulation was conducted by root dipping method. Brown planthopper was feed on treated plant for two days and then the next day the Brown Planthopper was fed on rice plant without treatment until the eighth day. Test insects used are brown planthopper instar 4th. Observations of insect mortality test for ten days with an interval of every 24-hour observation. Susceptibility values for neem oil insecticide formulations were determined based on the resistance ratio (RR) by comparing the LC50 value of the field population and standard population. Result of the observations showed that the farmers in the Padamulya village, Cipunagara district generally using synthetic insecticides to control of this pest. The active ingredient of most mentioned was imidacloprid with a lot of different applications. The result of examination of insecticide formulation of neem oil 50 EC using the root dipping method showed that a slow death trend and the highest mortality at eight days after application. The LC50 value of neem oil 50 EC insecticide against brown planthopper in the field population (Cipunagara) was 0.36% and RR 0.98. The result indicated that brown planthopper origin from Cipunagara was still susceptible to neem oil 50 EC insecticide formulation.Keyword: Neem oil formulation, Susceptilbility, Resistance ratio, Brown Planthopper
Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Hedi Paramita; Lindung Tri Puspasari; Yusup Hidayat; Rika Meliansyah; Danar Dono; Rani Maharani; Unang Supratman
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.565 KB) | DOI: 10.24198/cs.v1i1.16995

Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Bioactivity Fraction of Methanolic Seed Extract of Barringtonia asiatica L. (Kurz.) (Lecythidaceae) Against Spodoptera litura F. (Lepidoptera: Noctuidae) Teddy Budiyansyah; Danar Dono; Rika Meliansyah; Unang Supratman
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.142 KB) | DOI: 10.24198/cs.v1i2.19755

Abstract

Water seed extract of Barringtonia asiatica was used as fish poison by traditional community. This study aims to determine the bioactivity of methanolic seed extract fraction of B. asiatica against S. litura. The extract tested was spread evenly on the leaf surface by using microsyringe. The experiment consisted of 12 treatments and 3 replications. Treatments were concentration of 0.3% and 0.1% of fraction 16, 17, 18, 19, 20 of methanolic seed extract of B. asiatica, and concentration of 0.264% methanolic seed extract of B. asiatica; and control. The results showed that the fraction 19 (20% EtOAc and 80% MeOH eluent) of methanol extract of B. asiatica seed has the highest bioactivity against the larvae of S. litura. The fraction 19 of methanolic seed extract of B. asiatica had LC50 value of 0.182% at 12 days after treatment. In addition, the 19 fraction of methanolic seed extract of B. asiatica decreased food intake, weight and lengthened development time of larvae.Keywords : Barringtonia asiatica, bioactivity, fraction, Spodoptera litura.
Effect of Neem Oil Formulation on the Population of Soil Fungi and Disease Intencity of Cercospora Leaf Spot (Cercospora capsici) on Chilli Plants (Capsicum annuum) Sri Hartati; Danar Dono; Rika Meliansyah; Mukhamad Agung Yusuf
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.176 KB) | DOI: 10.24198/cs.v1i2.19753

Abstract

Soil fungi and cercospora leaf spot are soil inhabitants and plant disease, respectively, that usually occur on chilli grown area. Formulation of neem oil was reported to have a role as an environmentally friendly botanical pesticide. This research was objected to studying the effect of neem oil formulation on the population of soil fungi and its suppression on cercospora leaf spot intensity of chilli crop. The field experiment was arranged in the randomized complete block design. The treatments were the concentration of neem oil formulation, i.e. 0%, 1%, 1.25%, 1.50%, 1.75%, and 2.0%. In vitro experiment on the suppression of neem oil formulation on the predominant soil, fungi were arranged in the completely randomized design. The results showed that neem oil formulation did not affect the soil fungi population and cercospora leaf spot intensity. However,2.0% neem oil had the lowest soil population. Neem oil formulation at 1.75% caused the highest suppression on TMa01 isolate (49.81%), and 1.25%, 1.50%, 1.75% and 2.0% of neem oil formulation caused more than 50% suppression on TMf01 isolate in vitro. Neem oil formulation at 2.0% caused the highest suppression on cercospora leaf spot intensity (46.34%).Keywords: Azadirachta indica, botanical pesticide, neem oil
The effectiveness of soursop seed (Annona muricata L.) agains Callosobruchus maculatus F. (Coleoptera: Bruchidae) Rama Ginanjar Gilang; Nenet Susniahti; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.846 KB) | DOI: 10.24198/cs.v1i1.16994

Abstract

Callosobruchus maculatus F. (Coleoptera: Bruchidae) one of the main pests that cause damage to mung beans in storage areas. This research was conducted to determine the effective dose from soursop seed (Annona muricata) powder resulting mortality and emphasizing the amount of imago on the first-generation C. Maculatus. This research was conducted at Plant Pest Laboratory, Department of Plant Pests and diseases, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor using experimental method of Complete Random Design (CRD) with 6 treatments and 5 replications. The treatments were tested using 5 doses of A. muricata seed powder with 1 grams, 3 grams, 5 grams, 7 grams, and 9 grams doses, and added with control or without treatment. The results showed that the dose of 5 grams A. muricata seed powder showed the mortality of C. maculatus  by 94% at 8 days after infestation, emphasizing the amount of eggs which is placed  C. maculatus of mungbean seed 77,64%. Emphasis on the emergence of the first generation of imago in the mung bean seeds is 74.67%. The lowest of loss weight mung beed seed amount 1,33%.Keywords: Annona muricata, Callosobruchus maculatus, Soursop Seed Powder, Mortality
Laboratory Evaluation of Neem formulation bioactivity against Crocidolomia pavonana F. larvae Raden Arif Malik Ramadhan; Neneng Sri Widayani; Lindung Tri Puspasari; Yusup Hidayat; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.31 KB) | DOI: 10.24198/cs.v1i1.20334

Abstract

This study aims to evaluate the bioactivity of the formulation of Neem 50 EC  against Crocidolomia pavonana larvae in the laboratory. The study using six treatments and three replications. The treatment consisted of the formulation of neem 50 EC at concentrations of 0.3%, 0.5%, 0.8%, 1.3%, 2.3%, and control. The treatment was done by leaf feeding method and tested on C. pavonana instar II. The results of the research showed that the formulation of neem 50 EC at concentration of 2.3% caused mortality of C. pavonana larvae with mortality rate of 95% and has LC50 value aqual to 0.83%. Neem formulation can inhibit the development of C. pavonana larvae from instar I to instar IV, decrease food consumption, and decrease the dry weight of C. pavonana larvae instar IV.Keywords: Azadirachta indica, Bioactivity, Crocidolomia pavonana, neem formulation
Biologi dan Kemampuan Memangsa Paederus fuscipes Curtis (Coleoptera: Staphylinidae) terhadap Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) Sudarjat Sudarjat; Argo Utomo; Danar Dono
Agrikultura Vol 20, No 3 (2009): Desember, 2009
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.267 KB) | DOI: 10.24198/agrikultura.v20i3.959

Abstract

Bemisia tabaci merupakan hama tanaman di beberapa sentra produksi sayuran hingga mengakibatkan kerugian ekonomi sampai 100 %. Paederus fuscipes merupakan predator B. tabaci pada beberapa tanaman di Kecamatan Ciwidey, Bandung. Penelitian bertujuan untuk mengetahui biologi dan kemampuan memangsa imago P. fuscipes jantan terhadap nimfa B. tabaci. Percobaan menggunakan Rancangan Acak Kelompok yang terdiri atas lima perlakuan dan 5 ulangan. Perlakuan terdiri atas: infestasi 10, 20, 40, 80, dan 160 nimfa B. tabaci per satu imago P. Fuscipes. Hasil pengamatan biologi menunjukkan bahwa siklus hidup P. fuscipes berkisar antara 38-75 hari. Fase telur berkisar 4-7 hari, larva instar-1 berkisar 4-5 hari, larva instar-2 berkisar 6-9 hari, pra-pupa berkisar 2-3 hari, pupa berkisar 3-5 hari, dan lama hidup imago berkisar 19-46 hari. P. fuscipes memperlihatkan tanggap fungsional terhadap peningkatan kepadatan B. tabaci sebagai mangsa. Jumlah pemangsaan nimfa B. tabaci tertinggi terjadi pada kepadatan 160 nimfa, yaitu 101,1 dan 100,1 nimfa B. tabaci per imago P. fuscipes, masing-masing untuk periode pagi dan sore. Kecepatan memangsa imago P. fuscipes terhadap nimfa B. tabaci yaitu berkisar 0,83-8,17 nimfa per jam pada siang hari, dan 0,75-8 nimfa per jam pada malam hari.
Aktivitas Residu Ekstrak Biji Barringtonia asiatica (L.) Kurz. terhadap larva Crocidolomia pavonana F. (Lepidoptera : Pyralidae) Danar Dono; Rismanto Rismanto
Agrikultura Vol 19, No 3 (2008): Desember, 2008
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.251 KB) | DOI: 10.24198/agrikultura.v19i3.999

Abstract

Penelitian rumah plastik ini bertujuan untuk mengetahui hubungan antara kepadatan populasi hama kutu daun persik (Myzus persicae Sulz.), tingkat kerusakan daun dan kehilangan hasil cabai merah (Capsicum annuum). Delapan taraf kepadatan populasi M. persicae (0, 2, 4, 8, 16, 32, 64, dan 128 ekor / tanaman) masing-masing diinfestasikan pada tanaman cabai pada fase pertumbuhan awal dan fase pembungaan awal.  Hasil penelitian menunjukkan bahwa  populasi M. persicae dan tingkat kerusakan daun oleh hama tersebut berhubungan erat dengan kehilangan hasil cabai merah. Pada tanaman yang diinfestasi M. persicae saat fase pertumbuhan awal, hubungan antara kepadatan populasi (X) dengan kehilangan hasil (Y) mengikuti persamaan garis regresi Y = 19,1865 + 0,3568 X dengan keefektifan menduga sebesar 73,85% dan koefisien kerusakan sebesar 0,8724 g/ekor. Persamaan garis untuk tanaman yang diinfestasi M. persicae saat fase pembungaan awal adalah Y = 19,8504 + 0,3181X dengan keefektifan menduga  62,18 % dan koefisien kerusakan 0,7179 g/ekor. Hubungan antara tingkat kerusakan daun dengan kehilangan hasil pada tanaman cabai yang diinfestasi M. persicae saat fase pertumbuhan awal mengikuti persamaan garis regresi Y = 25,93 + 64,51 X1 + 0,26  X2 – 2,27 X3 {(Y = kehilangan hasil (%); X1 = tingkat kerusakan daun oleh M. persicae (%); X2 = populasi awal M. persicae (ekor/tanaman) dan X3 = waktu pengamatan (minggu setelah infestasi)} dengan  keefektifan menduga 78,75 % dan koefisien kerusakan 1,577 g/% kerusakan daun. Persamaan garis untuk tanaman cabai yang diinfestasi M. persicae saat fase pembungaan awal adalah Y = 25,59 + 1164,87 X1 + 0,08 X2 – 4,60 X3, dengan keefektifan menduga 79,18 % dan koefisien kerusakan 3,72  g/% kerusakan daun.