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Toxicity of Neem Cake Extract (Azadirachta indica) to Aphis glycine on Soybean Plant Ratmaneli Ratmaneli; Danar Dono; Vira Kusuma Dewi
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.865 KB) | DOI: 10.24198/cropsaver.v2i1.22722

Abstract

Aphis glycine is one of the soybean pests that can develop rapidly and cause yield loss. Neem is a plant known as botanical incesticide. This study tested the toxicity of neem cake against A. glycine and its effect on increasing tannin content in the soybean plant. The effect of neem cake extract to the mortality of A. glycine was tested by spray method and root dipping method. The application was carried out on four-week-old soybean plants with 10 individual of adult A. glycine as test insects.  Observations were carried out every day for 8 DAT (day after treatmen) by counting the population of A. glycine. The testing of tannin levels and azadirachtin content  is done by applying neem cake extract using root dipping method. The treatment is divided into two groups, which are infested plants and non-invested plants by A. glycine. The leaf of soybean (fourth day after application) was taken as samples for tannin and azadirachtin analysis. The results showed that the extract of neem cake at the spray method caused the death of A. glycine up to 97% at a concentration 2.5%. The root dipping method could make mortality of nymphs (new born) 100% at concentrations of 0.1% and 0.125% at 8 DAT , but cannot make mortality to adult of A. glycine. The tannin content in soybean plants (with A. glycine and without A. glycine invested) was increased after 0.125% neem cake application. Azdirachtin uptake was not detected at concentrations of 0.125% and 0.250% at 5 DAT. Neem cake extract can suppress A. glycine population in soybean plants and increase of tannin content in plants. 
Bioactivity of Crude Extract of Cashew Nut Shell (Anacardium occidentale L.) against Cabbage Head Caterpillar (Crocidolomia pavonana F.) Mia Sukma Dewi; Danar Dono; Sri Hartati
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.142 KB) | DOI: 10.24198/cs.v1i2.19913

Abstract

The crude extract of Anacardium occidentale was reported to have insektisidal activity against Cricula trifenestrata (Lepidoptera). Althought, the extract has not been tested against Crocidolomia pavonana (Lepidoptera). A research was carried out to test the effect of cashew nut shell extract on larvae and fecundity of adult female of C. pavonana. Toxicity of the extract tested by using feeding method to the larvae. The experiment arranged in the complete randomized design (CRD), consisted of 7 treatments and 3 repetitions. The treatments were the concentration of the extract i.e. 0.8%, 0.6%, 0.4%, 0.2%, 0.1%, and 0.05%. The same treatment methode was used to test the fecundity of C. pavonana female that developed from larval treated extract at concentrations of 0.16 % (LC30), 0.30 % (LC50), 0.45 % (LC70), and control. The results of the experiment showed that the extract of A. occidentale was toxic to larvae of C. pavonana with LC50 values of 0.30 % at six days after application. The extract of A. occidentale have an antifeedant effect and lengthened the development time of the larvae. The concentration range 0.16 % - 0.45 % reduced the weight of pupa, lengthened emergence time of adult, slowed down the first oviposition, reduced the egg production, shortened the life span of adult female and male, shortened the periode of oviposition of adult female, and decreased the fertility. Keywords: Crude extract of Anacardium occidentale, Crocidolomia pavonana, toxicity, fecundity
Effect of Temperature, Storage Time, The Residual Test of Neem Oil Formulation (Azadirachta indica A. JUSS) and Bitung Formulation (Baringtonia asiatica) to Its Toxicity Against Large Cabbage Heart Caterpillar (Crocidolomia pavonana F) Neneng Sri Widayani; Afifah Nashirotul Haq; Lindung Tri Puspasari; Yusup Hidayat; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.433 KB) | DOI: 10.24198/cs.v1i1.16996

Abstract

Effectiveness of pesticide affected by many factors such as physical environment and duration of storage. This study aims to determine the effect of temperature and storage time of formulation of neem 50 EC and residual effect of formulation of neem 50 EC and formulation of bitung 30 SC against C. pavonana larvae. The formulations tested on the experiment of effect storage time and temperature test using the newly prepared, neem formulation has been stored for 19 months at room temperature 24.6-31.7oC, and stored in low temperature (4oC) as well as controls. The toxicity of the neem oil formulation was tested at concentrations of 1%, 2%, and 3%. The experiment of residual effect was performed by spraying of the formulation on broccoli leaf, then the leaf samples were taken on days 0,1,2,3,5,7,9,11, and 13 days after application of insecticide. The results of the research indicated that storage at low temperatures (temperature 4oC) for 19 months can maintain the toxicity of the neem formulation to C. pavonana larvae. while storage at room temperature for 19 months resulted in a decreased toxicity. The residual activity of the neem formulations at concentration of 0.8% and 2.4% were not caused mortality of C. pavonana larvae at the residual age of 13.34 days and 14.66 days. The activity of Bitung formulation at concentration of 1.1% and 2.8% were not caused mortality of C. pavonana larvae at the residual age of 11.33 days and 16.26 days.
The Effectiveness of Neem Cake (Azadirachta indica) Water Extract against Aphids (Aphis gossypii) on Red Chili Plant Lathifah Azizah; Ceppy Nasahi; Danar Dono
CROPSAVER Vol 4, No 2 (2021)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v4i2.36634

Abstract

Aphis gossypii is one of the factors that reduce chili production which acts as a pest and virus vector. This research aimed to determine the effectiveness of the application of neem cake water extract to A. gossypii on red chili plants. This research consisted of 2 experimental stages, namely in the laboratory and the greenhouse trials. The experimental design in the laboratory used a completely randomized design of neem cake water extract with treatments is A (Control), B (0.3%), C (0.6%), D (1.2%), E (2.4%), F (4.8%). Each treatment replicate 4 times. Aplication of water neem cake extract by dipping method of chilli leaf. After the leaf feed air dried, then the leaf put into plastic box and invested with 10 nymph of A. gossypii. While the experiments in the greenhouse used a randomized block design of neem cake water extract treatments were A (Control), B (LC50), C (1.5xLC50), D (2xLC50), E (2.5xLC50), F (3xLC50). The concentration of neem extract in the greenhouse experiment based on result of experiment at laboratory. Adults of A. gossypii as much as 20 were invested on every red chili plant. Application of neem cake water extract on red chili plants once every 4 days. The results showed that neem cake water extract effective to  A. Gossypii with 0.14% as LC50. Neem cake water extract at concentration of 1.5xLC50 (0.21%) was effective suppressing A. gossypii population on red chili plants.
Toxicity of four kind plant extracts (Ageratum conyzoides L., Barringtonia asiatica (L.) Kurz., Melia azedarach L., Tephrosia vogelii Hook F.) against brown planthopper (Nilaparvata lugens STAL.) Giannisa Febritami; N Usyati; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.611 KB) | DOI: 10.24198/cs.v1i1.16970

Abstract

One of the main pests that attack rice plants is the brown planthopper (BPH), Nilaparvata lugens. Awareness of the negative effects of synthetic insecticides that have been used to control BPH has encouraged the use of natural resources for crop protection. The plant is one of natural source that can be used in the crop protection. Some plant species that have been known to have insecticidal activities include Ageratum conyzoides (Asteraceae), Barringtonia asiatica (Lechytidaceae), Melia azedarach (Meliaceae) and Tephrosia vogelii (Fabaceae). In this study toxicity test of the plant extracts was carried out on BPH, and its phytotoxicity was observed in rice plants. In the toxicity test of the plant extracts against N. lugens and the phytotoxicity test on rice plants used 2 concentrations namely 0.1% and 0.5% from each plant extract, while the antioviposition test used a concentration of 0.5%. The results showed that the methanolic extract of T. vogelii with a concentration of 0.5% caused the highest mortality of brown plant hopper nymphs of 42.5% and at concentration of 0.5% the four plant extracts did not inhibit oviposition activity of N. lugens female and did not phytotoxic on rice plants.Keywords: Toxicity, anti oviposition, plant extract, brown planthopper
The Effect of Neem Seed Oil (Azadirachta indica) and Clove Leaf Oil (Syzygium aromaticum) Mixture on Cabbage Head Caterpillars (Crocidolomia pavonana) Utami Dwi Ginasti; Danar Dono; Toto Sunarto
CROPSAVER Vol 3, No 2 (2020)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v3i2.29855

Abstract

Crocidolomia pavonana is one of the important pest in Brassicaceae family. The used of botanical pesticides such as neem and clove can be used as alternative control for C. pavonana. This study aimed to examine the effect of neem oil, clove oil, and its mixture to C. pavonana. This experiment was conducted from November 2019 to May 2020 at the Laboratory Pesticides and Environmental Toxicology of the Department of Plant Pests and Diseases of the Faculty of Agriculture, Universitas Padjadjaran. The experimental method used in this research was Randomized Block Design with 21 treatments, i.e control, neem oil, clove oil, and its mixture at concentrations of 0,1%, 0,25%, 0,5%, and 1%, each treatments was replicated 3 times. The result of this experiment showed that single or mixed treatments (Neem : Clove) at consentration of 1% caused 100% larvae mortality. Mixed treatment at ratio 1:1 could inhibits the formation of pupa and imago, and could prolong the development time of larvae in instar II-III and instar II-IV. In addition, mixed treatment at ratio 2:1 was also prolong the development time of larvae in instar II-pupa, reduce food consumption, inhibit the formation of imago, and reduce the number of eggs C. pavonana.
Cases of Fall Army Worm Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) Attack on Maize in Bandung, Garut and Sumedang District, West Java. Yani Maharani; Vira Kusuma Dewi; Lindung Tri Puspasari; Lilian Rizkie; Yusup Hidayat; Danar Dono
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.498 KB) | DOI: 10.24198/cropsaver.v2i1.23013

Abstract

The toxicity of neem (Azadirachta indica), citronella (Cymbopogon nardus), castor (Ricinus communis), and clove (Syzygium aromaticum) oil against Spodoptera frugiferda Danar Dono; Yusup Hidayat; Tarkus Suganda; Syarif Hidayat; Neneng Sri Widayani
CROPSAVER Vol 3, No 1 (2020)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.464 KB) | DOI: 10.24198/cropsaver.v3i1.28324

Abstract

An important pest in corn plantation recently reported in Indonesia was Spodoptera frugiperda, a polyphagous pest that have a fast spread rate and caused high damage on young corn. One control that able to use is botanical insecticide. This experiment aims to determine the toxicity of neem, jatropha, clove, and citronella oil (concentrations of 0.1% and 0.05%) against S. frugiferda in corn. Tests carried out using the feeding assay or residue on corn leaves (size 5 x 5 cm). Then, the leaf treatment was given to 10 larvae of S. frugiferda (instars 2) for 48 hours. After that, the feed replaced with untreated feed. The test results showed that concentration neem oil of 0.1% caused the highest mortality of S. frugiferda. Neem oil exposure is also able to inhibit the time of larval development, reduce the percentage of feed consumption, and the weight of pupa the S. frugiferda. Other vegetable oils exposure gave lower mortality in the test insects and some treatments were not significantly different from the controls on the parameters of larval development, pupae weight, and percentage of feed consumption. Neem oils showed strong toxicity against S. frugiperda larvae with  Lethal Concentration 50 (LC50) of 0.079 % (CI (95%) 0.066 – 0.095%) and LC95 of 0.338% (CI (95%) 0.024-0.567%).
Toxicity of Barringtonia asiatica Seed Extract Against Chilo sachariphagus Rohendi Rohendi; Danar Dono; Andang Purnama
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.911 KB) | DOI: 10.24198/cs.v2i1.22297

Abstract

Barringtonia asiatica is one of the plants that the seeds extracts has insecticidal properties. However, the bioactivity of this seed extract has not been tested against Chilo sacchariphagus larvae which is the main pests in sugar cane. The treatment was carried out at several levels of concentration of methanolic seed extract of B. asiatica. The 15 cm of fresh sugarcane shoot dipping into the extract solution in some second. After the solution of extract on the sugarcane shoot dried, the first instar of larvae infested to the sugarcane shoot and then put into 20 cm test tube with 3 cm of diameter.  The duration of feeding on treated sugarcane shoot was two days and on the following day the larvae were fed on sugar cane shoots until the survived larvae reached the 5th instar. The results of the experiment indicated that B. asiatica seed extract was toxic to C. sacchariphagus with an LC50 value of 0.398% at 16 days after treatment. Besides toxic, B. asiatica seed extract also has atifeedant activity, and inhibits the growth of immature of C. sacchariphagus in all tested concentrations.Keyword: Toxicity, antifeedant, lethal concentration, Barringtonia asiatica, Chilo sachariphagus
Toxicity of Methanolic Seed Extract of Barringtonia asiatica L. (Kurz) (Lecythidaceae) from Different Place in Indonesia Against Spodotera litura F (Lepidoptera: Noctuidae) Danar Dono; Irpan Permana
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.709 KB) | DOI: 10.24198/cs.v1i2.19754

Abstract

One of the potential plants to be developed as insecticide is Barringtonia asiatica (Lecythidaceae). Phytochemicals of the same species plant is different in different place and time. This research was conducted to determine toxicity methanolic seed extract of Barringtonia asiatica from different field in Java i.e. Pangandaran, Sindangkerta, Bandung, Jatinangor, and Yogyakarta against Spodoptera litura. The evaluation of toxicity was carried out using feeding method. The result indicated that seed extract of B. asiatica showed value of toxicity from higher to lower were extract of B.asiatica from Pangandaran, Jatinangor, Sindangkerta, Bandung, and Yogyakarta respectively. The extract of B. asiatica showed rendement from higer to lower were extract of B.asiatica from Pangandaran (37.8%), Sindangkerta (36.1%), Yogyakarta (29.3%), Bandung (26.5%) and Jatinagor (25.8%). Methanolic seed extract of B.asiatica from Pangandaran showed the highest toxicity and rendement compared from the other place, with LC at 0.53%. In addition of toxic property, the extract of B. asiatica seed decreased of 50 leaf consumption and pupal weight and lenghtened development time of tested larvae. Keywords:  Biology, extract, toxicity, Barringtonia asiatica, Spodoptera  litura
Co-Authors Abraham Suriadikusumah Afifah Nashirotul Haq Ainun, Khairunissa Andang Purnama Andhita Nadhirah Argo Utomo C. Pickerling Ceppy Nasahi DJOKO PRIJONO Eka Rahayu Setyaningsih Emelda Anggraini Endah Yulia Endah Yulia Entun Santosa Entun Santosa Entun Santosa - Erawati, Alfira Dewi Ernaldi Eka Nanda Fadhilah Rahmah Aprianti Fathussalam, Muhammad Fauziaty, Muthia Riefka Febritami, Giannisa Fhera Hardiani Fitri Widiantini Fizrul Indra Lubis Frida P. Inangsih - Giannisa Febritami Giffari, Fahri Rijal Gigih Ibnu Prayoga Gilang, Rama Ginanjar Haq, Afifah Nashirotul Haryadi, Dudi Hedi Paramita IDAR IDAR IKHA MUSLIKHA Indah Meutia Arisanti Irman Eka Septiarusli Irpan Permana Irsyad, Andika Muhammad Ivan Febriana Iwan Setiawan Jabbar, Muhammad Aqshal Azizil Kiki Haetami kinya toriyama Lathifah Azizah Lilian Rizkie Lindung Tri Puspasari Maulana, Raihan Rahmat Meliyansyah, Rika Mia Sukma Dewi Mochamad Arief Soleh Muhammad Maksum Mukhamad Agung Yusuf N Usyati Nanda, Ernaldi Eka Narendra, Nathanael Dimas Nasrudin Nasrudin Nasya Nabila Neneng S Widayani Neneng Sri Widayani Neneng Sri Widayani Neneng Sri Widayani Nenet Susniahti Nenet Susniahti Neni Rostini Neni Rostini Nono Carsono Noor Istifadah Nurfadza, Alysha Rianty Nurul Khumaida Paramita, Hedi Purnawan, Pupung Putri, Ghifa Alghifaira R. Arif Malik Ramadhan Raden Arif Malik Ramadhan Rama Ginanjar Gilang Ramadhan, R. Arif Malik Rani Maharani Rani Maharani Ratmaneli Ratmaneli Retno Wulansari Rika Meliansyah Rismanto Rismanto Rohendi Rohendi Rusydan, Muhammad Ardillah Safri Ishmayana Safri Ishmayana Safri Ishmayana Safri Ishmayana Santika Sari Santika Sari Siska Rasiska SIska Rasiska, SIska Sri Hartati Sudarjat Sudarjat Sudarjat Sudarjat Susanti, Rista Susanto, Ainun Nandini Putri Susniahty, Nenet SYAFRI ISMAYANA Syarif Hidayat Syarif Hidayat Syariful Mubarok Tarkus Suganda TATI NURHAYATI Teddy Budiyansyah Toto Sunarto Toto Sunarto Unang Supratman Usyati, N Utami Dwi Ginasti Vira Kusuma Dewi Wahyu Daradjat Natawigena, Wahyu Daradjat Widayani, Neneng Sri Widayani, Neneng Sri Yani Maharani Yenny Mulyani Yogas Dwi Pratiwi Yusuf Hidayat Yusuf Hidayat Yusup Hidayat Yusup Hidayat Yusup Hidayat Yusup Hidayat Yusup Hidayat Yuwono, Indra