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All Journal Jurnal Kimia (Journal of Chemistry) Jurnal Veteriner Jurnal Kedokteran Syiah Kuala Folia Medica Indonesiana Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya ANNALES BOGORIENSES Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Jurnal Natural Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika
Eddy Bagus Wasito
Departemen Mikrobiologi Kedokteran, Fakultas Kedokteran, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary Other

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In Vitro Antibacterial Activity of Averrhoa bilimbi Leaves Ethanol Extract Against Salmonella typhi Margaretha Nathania; Eddy Bagus Wasito; Nurina Hasanatuludhhiyah
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 14 No. 1 (2023): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V14I12023.43-47

Abstract

Highlights:1. Typhoid fever, a major health problem for developing countries, requires alternative therapy to reduce drug resistance.2. A. bilimbi, a native plant of Indonesia, has antibacterial potential, which is effective against S. typhi in vitro. AbstractIntroduction: Some native plants in Indonesia have potential effects on health and medication. This study aimed to determine the potential antibacterial effect of Averrhoa bilimbi (A. bilimbi) leaves ethanol extract against Salmonella typhi (S. typhi) bacteria. Methods: This was an experimental study. The antibacterial potency of A. bilimbi leaves was measured against S. typhi in vitro. Different concentrations of the leaves’ ethanol extract were prepared. The minimum inhibitory concentration (MIC) was evaluated by a macro-dilution method using Mueller Hinton broth. The minimum bactericidal concentration (MBC) value was observed by subculturing the specimen from the previous dilution tube to the nutrient agar.Results: The MIC of A. bilimbi leaves ethanol extract against S. typhi bacteria could not be determined because all the treatment tubes’ colors were dark and turbid. Meanwhile, the MBC value was at 500 mg/ml.Conclusion: A. bilimbi leaves extract had potential bactericidal effects against S. typhi with a MIC value that could not be determined.
HIV Genotype Analysis from HIV Infected Patients in East Java Area Yulia Sari Ismail; Soetjipto Soetjipto; Eddy Bagus Wasito; Nasronudin Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.358 KB)

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
Proportions of Group B Streptococcus Isolation from Pregnant Women’s Vaginal and Rectal Swab Specimens at a Tertiary Hospital in Surabaya, Indonesia Ivanna; Eddy Bagus Wasito; Kartuti Debora
Folia Medica Indonesiana Vol. 59 No. 1 (2023): March
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.073 KB) | DOI: 10.20473/fmi.v59i1.11708

Abstract

Highlights: Rectal and vaginal swab specimens were collected from pregnant women, and there was no significant difference in the proportions of group B Streptococcus isolation. Combined vaginal and rectal swab cultures provide a higher isolation of group B Streptococcus. Abstract : Group B Streptococcus is a Gram-positive bacterium found in women. It causes high-risk mortality in pregnant women, newborns, and the elderly. This study aimed to compare group B Streptococcus (GBS/Streptococcus agalactiae) proportions from different collection sites (vaginal and rectal swabs). This was an analytic observational study with a hospital-based cross-sectional design. A total of 74 swabs were taken from 37 pregnant women at 35–37 weeks of gestation. Each participant provided a vaginal swab and a rectal swab, which were cultured in Todd Hewitt broth, blood agar, and CHROMagar. The specimens were subsequently identified using the VITEK 2 system. The GBS isolation percentages from the vaginal and rectal swab specimens were determined to be 13.5% and 8.1%, respectively. The McNemar test had a result of 0.697, and the Cohen’s kappa test had a result of 0.165. To conclude, there was no significant difference in GBS isolation proportions between the vaginal and rectal swab cultures. Combined vaginal and rectal swab cultures were required to increase GBS isolation from pregnant women.  
Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain Ratna Kusumawati; Eddy Bagus Wasito; Arthur Pohan Kawilarang; Eko Budi Koendhori
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 7 No 2 (2023): Journal Qanun Medika Vol 07 No 02
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v7i2.18606

Abstract

Mycosis is often misdiagnosed as Tuberculosis (TB) with negative sputum smear results or Tuberculosis recurrent. In the last two-decade, mycosis increases dramatically as HIV and immunocompromised incidence rates increased. Even though, the prevalence data of mycosis in TB patient is less researched, the identification using KOH often give a false negative result. To compare the result of fungal identification using KOH and PAS staining. This study is observational analytical using a cross-sectional design with 29 TB samples that have been diagnosed with Ziehl-Neelsen (ZN) stain positive in RSUD Dr. Soetomo. The sputum of every sample was colored with KOH and PAS and observed by the researcher and microbiology analyst. The results of this study were 22 sputa identified with Candida sp positive and 12 sputa with non-Candida positive using KOH staining. Meanwhile, using PAS staining, there were 25 sputa identified as Candida sp positive and 22 sputa as non-Candida positive. The congruency test between KOH and PAS has a fair result for both identifying Candida sp (κ = 0.298; significance = 0.069) and non-Candida (κ = 0.240; significance = 0.095). The identification of candida and non-candida in TB patients using KOH and PAS has a fair congruency result. The PAS staining has better identification results both in identifying candida and non-candida rather than KOH staining. 
Sepsis caused by Salmonella serovar paratyphi B in immunocompromised patient with kasabach-merritt syndrome in dr. Soetoro general academic hospital Surabaya: a case report Suharyadi Sasmanto; Wasito, Eddy Bagus
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 1 (2023): Available online : June 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i1.30

Abstract

Introduction: Salmonella species are recognized worldwide as a common cause of childhood infections, particularly gastroenteritis, bacteremia, and enteric fever. Some salmonella infection cases, especially those caused by Salmonella paratyphi B, which are rare, are reported to cause sepsis in children and neonates. The treatment of Salmonella paratyphi B-induced sepsis is challenging, particularly in immunocompromised babies as in Kasabach-Merritt Syndrome (KMS). This case report aims to report the case of a 1.7-year-old girl who had Salmonella serovar paratyphi B-induced sepsis and presented with KMS. Case description: A 1.7 years old girl has complaints of fever for 5 days prior to admission to the hospital with a history of a growing haemangioma in the abdominal area since the first 40 days since birth. The patient was diagnosed with KMS. During the period of hospitalization, there were no complaints of fever, therefore, the patient was discharged. The next day, the patient complained of persistent fever and general weakness. Vital signs examination revealed tachycardia and hyperthermia. Laboratory results showed the hemoglobin was 8.7 gr/dL, leucocyte 20.640/uL, neutrophil 65%, lymphocyte 20.5%, platelet 14.000 uL, CRP 14.22 mg/dL. The patient was diagnosed with Kasabach-Merritt Syndrome (KMS) and has been in control as an outpatient and received oral therapy with methylprednisolone, propranolol and tranexamic acid. The patient was readmitted for chemotherapy with bleomycin and vincristine as part of the KMS treatment regimen. Empiric antibiotic (200 mg ampicillin i.v.) also administered as Salmonella sp. Bacteria was found on the blood culture. Conclusion: Salmonella paratyphi B is a rare infection, the symptoms are typically mild. Rapid identification of bacterial infection and antimicrobial susceptibility testing is crucial for effective treatment.
Comparison between Exposure of Ciprofloxacin and Cefotaxime on Developing of Escherichia coli ESBL Amin, Muhammad; Wasito, Eddy Bagus; Triyono, Erwin Astha
Folia Medica Indonesiana Vol. 56 No. 2 (2020): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.266 KB) | DOI: 10.20473/fmi.v56i2.21203

Abstract

This study aimed to compare ciprofloxacin and cefotaxime exposure to develop ESBL producing Escherichia coli (E. coli). A total of 16 isolates of cefotaxime sensitive E. coli and ciprofloxacin were exposed to ciprofloxacin and cefotaxime for 14 days using the Kirby-Bauer antibiotic disc diffusion method. Colonies that grew on the edge of the inhibiting zone were exposed each day by the same method. Furthermore, we observed the occurrence of resistance to cefotaxime as ESBL screening test. Isolates were resistant, the following day the ESBL was confirmed by the Modified Double Disk Sinergy Test (MDDST) method using Cefotaxime (CTX), Ceftazidime (CAZ), Aztreonam (ATM), and Amoxilin Clavulanate (AMC) antibiotic discs. From 16 isolates of ESBL producing E. coli exposed to ciprofloxacin, it was obtained 4 (25%) to ESBL E. coli. ESBL production occurred after E. coli was exposed to ciprofloxacin on days 5, 6, 7, and 12. While those exposed to cefotaxime none becomes ESBL E. coli. There was no difference between ciprofloxacin and cefotaxime exposure to develop ESBL producing E. coli (p=0.101; Chi-square).
Effect of Glucose Induction on Biofilm Density in Clinical Isolate Acinetobacter baumannii Patients in Intensive Care Unit of Dr. Soetomo Hospital, Surabaya Lindarto, Wira W; Wasito, Eddy Bagus; Debora, Kartuti
Folia Medica Indonesiana Vol. 56 No. 2 (2020): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.508 KB) | DOI: 10.20473/fmi.v56i2.21230

Abstract

This study aimed to analyze the effect of glucose induction on the clinical isolate biofilm density of Acinetobacter baumannii. Thirteen clinical isolates of A. baumannii non biofilm forming were collected from non-DM patients who were treated at the ICU of Dr. Soetomo Hospital, Surabaya, was treated with the addition of 0.08% glucose, 0.15% glucose, 0.2% glucose, and 0.4% glucose in TSB growth media, followed by biofilm density examination with Tissue Culture Plate Method (TCPM) using 96 wells flatbottomed polyesterene tissue culture plate and read by autoreader ELISA with a wavelength of 630 nm (OD630). Biofilm density obtained was analyzed using ANOVA statistical analysis. The results of OD630 showed that the biofilm density increased significantly at the addition of 0.2% and 0.4% glucose. There was a significant increase in biofilm density at the addition of 0.2% and 0.4% glucose so that the management of blood sugar levels in ICU patients was needed before and when medical devices were installed.
Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA) Hidayah, Amaliyah Nurul; Wasito, Eddy Bagus; Debora, Kartuti; Basori, Achmad; Isnaeni, Isnaeni; Utomo, Budi
Folia Medica Indonesiana Vol. 55 No. 1 (2019): March
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.474 KB) | DOI: 10.20473/fmi.v55i1.24277

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has been resistant to various types of antibiotics, so it is not easy to be treated with antibiotics and needs other solutions. Javanese citronella oil distilled from the Cymbopogon nardus plant is proven to function as an antibacterial agent (bacteriostatic and bactericidal), fungicide and repellent. This study aimed to prove that there is a positive correlation between bacteriostatic and bactericidal effects with antibiofilm and anticolony spreading from Javanese citronella oil on MRSA. The intended antibiofilm is a barrier to biofilm formation and eradication. Bacteriostatic and antibiofilm effects were tested using microtiter plates assay, bactericidal effect test with subculture into the media and anticolony spreading effect test with spot inoculation in Tryptic Soy Broth media supplemented with 0.24% agar. The bacteriostatic effect test data were analyzed using paired t-test, bactericidal effect using the Friedman test, antibiofilm effect test using Kruskall-Wallis and the results of all the tests correlated using Pearson and Spearman correlation. The statistical significance used was p<0.05. The results showed that Javanese citronella oil had a bacteriostatic concentration of 0.02% (v/v) and bactericidal concentration of 0.78% (v/v). The Pearson correlation test showed that there was a negative correlation between bacteriostatic and bactericidal effects on biofilm formation with r = -0.956 (p = 0.000), but the correlation was positive for biofilm eradication with r = 0.918 (p = 0.000) and anticolony spreading with r = 1.000 (p = 0.000).
Perbandingan Distribusi Dan Pola Kepekaan Acinetobacter baumannii Terhadap Antimikroba Di RSUD Dr. Soetomo Surabaya Periode Januari–Maret 2015, April–Juni 2015, dan Januari–Maret 2016 Silvia Sutandhio; Agung Dwi Wahyu Widodo; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1782

Abstract

Acinetobacter baumannii is an environmental bacteria that can cause opportunistic infections such as ventilator-associated pneumonia, burn wound infection, urinary tract infection, and sepsis in immunocompromised patients. A. baumannii infections are troublesome because the bacteria resistance to many antimicrobial agents. Surveillance of distribution and sensitivity pattern of A. baumannii is best to be done frequently in hospitals.Venous blood as much as 10 milliliters was drawn aseptically and transferred into liquid medium Bactec® to be sent to Clinical Microbiology Laboratory of Dr. Soetomo General and Teaching Hospital. Medium that showed bacterial growth was subcultured on Blood Agar Plate and MacConkey Agar, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with semi-automated system BD Phoenix, and interpreted based onClinical and Laboratory Standards Institute 2015.A. baumannii collected on trimester January-March 2015 were 30 isolates, with 19 isolates being Multidrug Resistant(MDR) A. baumannii (63%) and 6 isolatesPandrug Resistant(PDR)A. baumannii (20%). Isolates from trimester April-June 2015 were 32 isolates, with 21 isolates being MDR A. baumannii (66%) and 2 isolates PDR A. baumannii (6%). Isolates from trimester January-March 2016 were 40 isolates, with 22 isolates being MDR A. baumannii (55%) and 2 isolates PDR A. baumannii (5%).A. baumannii mostly isolated from Intensive Care Unit, followed by Internal medicine ward, Pediatric ward, Surgery ward and Emergency Department. High sensitivity of A. baumanniion trimester January-March 2016 was to imipenem (50%), meropenem (50%), amikacin (53%), cotrimoxazole (53%), dan cefoperazone-sulbactam (55%).. MDRA. baumannii mostly isolated from ICU, which reflects high burden of antimicrobial use. At this moment, antimicrobial agents that can be used as empirical therapy for A. baumannii infection are imipenem, meropenem, amikacin, cotrimoxazole, and cefoperazone-sulbactam. The entire hospital member must implement the Infection Control and Prevention and Antimicrobial Stewardship Program to preventnosocomial infections byA. baumanniiand the emergence of MDRA. baumannii.
Antimikroba: Magic Bullet Versus Superbugs Silvia Sutandhio; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1807

Abstract

Magic bullet is a terminology used by Paul Ehrlich to refer to substance that selectively attacks microbes that cause infection without affecting human tissues. The concept of magic bullet is adopted in attempts to find antimicrobial drugs that is safe to human. Improper and excessive use of antimicrobial drugs has caused the emergence ofmicrobes that are resistant to various antimicrobial drugs (superbugs). Superbugs are recent healthcare and public health issues. This condition is exacerbated bythe discovery void of effective antimicrobial drug against superbugs. Health practitioners must perform prudent use of antibiotics in order to prevent transmission of and infection by superbugs.
Co-Authors Achmad Basori, Achmad Agung Dwi Wahyu Widodo Alimsardjono, Lindawati Ardhiya Puspita Arthur Pohan Kawilarang Asnia Zainuddin Bambang Hermanto Branandito Putra Budi Utomo Budiono Budiono Eko Budi Koendhori, Eko Budi Erwin Astha Triyono H.M. Soekry Erfan Kusuma Hanindita, Meta Herdiana Hardiani, Kartika Hardiono Hardiono I Nengah Wirajana I'tishom, Reny Isnaeni Isnaeni Ivanna Kartuti Debora Kartuti Debora, Kartuti Kazuo Sakka Kimura, Tetsuya Kuntaman Kuntaman Kusuma, Soekry Erfan Linda Dewanti Lindarto, Wira W Margaretha Nathania Maria Inge Lusida Muhammad Amin Nasronudin Nasronudin Neisya Intan Cahyaningtyas Agung Putri Ni Nyoman Tri Puspaningsih Nisa Amnifolia Niazta Nur Aisiyah Widjaja, Nur Aisiyah Nurina Hasanatuludhhiyah Nurwasis Nurwasis Nyilo Purnami Radita Yuniar Arizandy Ramadhani Ramadhani Ratna Kusumawati Roedi Irawan S.Pd. M Kes I Ketut Sudiana . Sakka, Kazuo Silvia Sutandhio Soekry Erfan Kusuma SOETJIPTO . Suharyadi Sasmanto Tetsuya Kimura Triffit Imasari WINARTO Wiwiek Tyasningsih Yulia Sari Ismail Zuhria, Ismi