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All Journal Jurnal Kimia (Journal of Chemistry) Jurnal Veteriner Jurnal Kedokteran Syiah Kuala Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya ANNALES BOGORIENSES Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Jurnal Natural Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Annales Bogorienses Folia Medica Indonesiana
Eddy Bagus Wasito
Departemen Mikrobiologi Kedokteran, Fakultas Kedokteran, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain Ratna Kusumawati; Eddy Bagus Wasito; Arthur Pohan Kawilarang; Eko Budi Koendhori
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 7 No 2 (2023): Journal Qanun Medika Vol 07 No 02
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v7i2.18606

Abstract

Mycosis is often misdiagnosed as Tuberculosis (TB) with negative sputum smear results or Tuberculosis recurrent. In the last two-decade, mycosis increases dramatically as HIV and immunocompromised incidence rates increased. Even though, the prevalence data of mycosis in TB patient is less researched, the identification using KOH often give a false negative result. To compare the result of fungal identification using KOH and PAS staining. This study is observational analytical using a cross-sectional design with 29 TB samples that have been diagnosed with Ziehl-Neelsen (ZN) stain positive in RSUD Dr. Soetomo. The sputum of every sample was colored with KOH and PAS and observed by the researcher and microbiology analyst. The results of this study were 22 sputa identified with Candida sp positive and 12 sputa with non-Candida positive using KOH staining. Meanwhile, using PAS staining, there were 25 sputa identified as Candida sp positive and 22 sputa as non-Candida positive. The congruency test between KOH and PAS has a fair result for both identifying Candida sp (κ = 0.298; significance = 0.069) and non-Candida (κ = 0.240; significance = 0.095). The identification of candida and non-candida in TB patients using KOH and PAS has a fair congruency result. The PAS staining has better identification results both in identifying candida and non-candida rather than KOH staining. 
Sepsis caused by Salmonella serovar paratyphi B in immunocompromised patient with kasabach-merritt syndrome in dr. Soetoro general academic hospital Surabaya: a case report Suharyadi Sasmanto; Wasito, Eddy Bagus
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 1 (2023): Available online : June 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i1.30

Abstract

Introduction: Salmonella species are recognized worldwide as a common cause of childhood infections, particularly gastroenteritis, bacteremia, and enteric fever. Some salmonella infection cases, especially those caused by Salmonella paratyphi B, which are rare, are reported to cause sepsis in children and neonates. The treatment of Salmonella paratyphi B-induced sepsis is challenging, particularly in immunocompromised babies as in Kasabach-Merritt Syndrome (KMS). This case report aims to report the case of a 1.7-year-old girl who had Salmonella serovar paratyphi B-induced sepsis and presented with KMS. Case description: A 1.7 years old girl has complaints of fever for 5 days prior to admission to the hospital with a history of a growing haemangioma in the abdominal area since the first 40 days since birth. The patient was diagnosed with KMS. During the period of hospitalization, there were no complaints of fever, therefore, the patient was discharged. The next day, the patient complained of persistent fever and general weakness. Vital signs examination revealed tachycardia and hyperthermia. Laboratory results showed the hemoglobin was 8.7 gr/dL, leucocyte 20.640/uL, neutrophil 65%, lymphocyte 20.5%, platelet 14.000 uL, CRP 14.22 mg/dL. The patient was diagnosed with Kasabach-Merritt Syndrome (KMS) and has been in control as an outpatient and received oral therapy with methylprednisolone, propranolol and tranexamic acid. The patient was readmitted for chemotherapy with bleomycin and vincristine as part of the KMS treatment regimen. Empiric antibiotic (200 mg ampicillin i.v.) also administered as Salmonella sp. Bacteria was found on the blood culture. Conclusion: Salmonella paratyphi B is a rare infection, the symptoms are typically mild. Rapid identification of bacterial infection and antimicrobial susceptibility testing is crucial for effective treatment.
Perbandingan Distribusi Dan Pola Kepekaan Acinetobacter baumannii Terhadap Antimikroba Di RSUD Dr. Soetomo Surabaya Periode Januari–Maret 2015, April–Juni 2015, dan Januari–Maret 2016 Silvia Sutandhio; Agung Dwi Wahyu Widodo; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1782

Abstract

Acinetobacter baumannii is an environmental bacteria that can cause opportunistic infections such as ventilator-associated pneumonia, burn wound infection, urinary tract infection, and sepsis in immunocompromised patients. A. baumannii infections are troublesome because the bacteria resistance to many antimicrobial agents. Surveillance of distribution and sensitivity pattern of A. baumannii is best to be done frequently in hospitals.Venous blood as much as 10 milliliters was drawn aseptically and transferred into liquid medium Bactec® to be sent to Clinical Microbiology Laboratory of Dr. Soetomo General and Teaching Hospital. Medium that showed bacterial growth was subcultured on Blood Agar Plate and MacConkey Agar, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with semi-automated system BD Phoenix, and interpreted based onClinical and Laboratory Standards Institute 2015.A. baumannii collected on trimester January-March 2015 were 30 isolates, with 19 isolates being Multidrug Resistant(MDR) A. baumannii (63%) and 6 isolatesPandrug Resistant(PDR)A. baumannii (20%). Isolates from trimester April-June 2015 were 32 isolates, with 21 isolates being MDR A. baumannii (66%) and 2 isolates PDR A. baumannii (6%). Isolates from trimester January-March 2016 were 40 isolates, with 22 isolates being MDR A. baumannii (55%) and 2 isolates PDR A. baumannii (5%).A. baumannii mostly isolated from Intensive Care Unit, followed by Internal medicine ward, Pediatric ward, Surgery ward and Emergency Department. High sensitivity of A. baumanniion trimester January-March 2016 was to imipenem (50%), meropenem (50%), amikacin (53%), cotrimoxazole (53%), dan cefoperazone-sulbactam (55%).. MDRA. baumannii mostly isolated from ICU, which reflects high burden of antimicrobial use. At this moment, antimicrobial agents that can be used as empirical therapy for A. baumannii infection are imipenem, meropenem, amikacin, cotrimoxazole, and cefoperazone-sulbactam. The entire hospital member must implement the Infection Control and Prevention and Antimicrobial Stewardship Program to preventnosocomial infections byA. baumanniiand the emergence of MDRA. baumannii.
Antimikroba: Magic Bullet Versus Superbugs Silvia Sutandhio; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1807

Abstract

Magic bullet is a terminology used by Paul Ehrlich to refer to substance that selectively attacks microbes that cause infection without affecting human tissues. The concept of magic bullet is adopted in attempts to find antimicrobial drugs that is safe to human. Improper and excessive use of antimicrobial drugs has caused the emergence ofmicrobes that are resistant to various antimicrobial drugs (superbugs). Superbugs are recent healthcare and public health issues. This condition is exacerbated bythe discovery void of effective antimicrobial drug against superbugs. Health practitioners must perform prudent use of antibiotics in order to prevent transmission of and infection by superbugs.
Distribusi dan Antibiogram MRSA dari Spesimen Darah selama Empat Semester di RSUD Dr. Soetomo Surabaya Silvia Sutandhio; Agung Dwi Wahyu Widodo; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 2 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i2.1812

Abstract

MRSA bloodstream infection is hospital-acquired problem that is difficult to treat. Bacteria spread to body organs, create multiple loci of infection, and survive in the presence of beta-lactam antibiotics. Study of distribution and antibiogram of MRSA from blood specimens was done in four semesters; i.e. July–December 2014, January–June 2015, July–December 2015, and January–June 2016. Blood of patients suspected for bloodstream infection was drawn aseptically, transferred into liquid medium, and sent to Clinical Microbiology Laboratory of Dr. Soetomo General Hospital. Medium with microbial growth was subcultured on solid medium, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with BD Phoenix, and interpreted based on Clinical and Laboratory Standards Institute 2015. Of total 107 MRSA isolates; 23 isolates (22%) were collected on July–December 2014, 12 isolates (11%) on January–June 2015, 25 isolates (23%) on July–December 2015, and 47 isolates (44%) on January–June 2016. MRSA mostly isolated from internal disease wards (45%). Isolates are sensitive to linezolid (91%), quinupristin-dalfopristin (83%), fosfomycin (82%), and vancomycin (80%). Trend of MRSA bloodstream infection is increasing. Although antibacterial agents against MRSA are available, it is best to prevent MRSA transmissions and infections.
Construction of pY-Af Vector for Expression of Thermostable α-L-Arabinofuranosidase in Saccharomyces cerevisiae Wirajana, I Nengah; Puspaningsih, Ni Nyoman Tri; Wasito, Eddy Bagus; Kusuma, Soekry Erfan; Kimura, Tetsuya; Sakka, Kazuo
Annales Bogorienses Vol. 14 No. 2 (2010): Annales Bogorienses
Publisher : BRIN

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Abstract

In this research, construction of expression vector for thermostable α-L-arabinofuranosidase in Saccharomyces cerevisiae BJ1824 was conducted. The Escherichia coli/S. cerevisiae shuttle vector, pYES2 was used as parental vector in construction. The abfA gene encoding α-L-arabinofuranosidase from Geobacillus thermoleovorans IT-08 was amplified by PCR, in which the plasmid pTP510 was used as a template. The amplification product was treated with SacI and XhoI and then subcloned to the pYES2 vector, which was previously digested with SacI and XhoI. The recombinant plasmid was designated as pY-Af and propagated first in E. coli Top10, and then transformed into S. cerevisiae BJ1824. For α-Larabinofuranosidase (AbfA) production, the yeast transformants were grown in YNBG selective medium and YPG rich medium, using galactose as an inducer. The AbfA activity was assayed by measuring the amount of p-nitrophenol (pNP) released from p-nitrophenyl-α-L-arabinofuranoside (pNPA) substrate at pH 6.0 and 70oC for 30 min. The recombinant AbfA activity was detected in either of culture medium (0.98%), cellassociated (14.17%) and intracellular (84.85%) when recombinant yeast was grown in YPG rich medium.
Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA) Hidayah, Amaliyah Nurul; Wasito, Eddy Bagus; Debora, Kartuti; Basori, Achmad; Isnaeni, Isnaeni; Utomo, Budi
Folia Medica Indonesiana Vol. 55, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has been resistant to various types of antibiotics, so it is not easy to be treated with antibiotics and needs other solutions. Javanese citronella oil distilled from the Cymbopogon nardus plant is proven to function as an antibacterial agent (bacteriostatic and bactericidal), fungicide and repellent. This study aimed to prove that there is a positive correlation between bacteriostatic and bactericidal effects with antibiofilm and anticolony spreading from Javanese citronella oil on MRSA. The intended antibiofilm is a barrier to biofilm formation and eradication. Bacteriostatic and antibiofilm effects were tested using microtiter plates assay, bactericidal effect test with subculture into the media and anticolony spreading effect test with spot inoculation in Tryptic Soy Broth media supplemented with 0.24% agar. The bacteriostatic effect test data were analyzed using paired t-test, bactericidal effect using the Friedman test, antibiofilm effect test using Kruskall-Wallis and the results of all the tests correlated using Pearson and Spearman correlation. The statistical significance used was p<0.05. The results showed that Javanese citronella oil had a bacteriostatic concentration of 0.02% (v/v) and bactericidal concentration of 0.78% (v/v). The Pearson correlation test showed that there was a negative correlation between bacteriostatic and bactericidal effects on biofilm formation with r = -0.956 (p = 0.000), but the correlation was positive for biofilm eradication with r = 0.918 (p = 0.000) and anticolony spreading with r = 1.000 (p = 0.000).
Comparation of Phenotypic and Genotypic Profile of Carbapenemase Producing Escherichia coli Sutandhio, Silvia; Budiono, Budiono; Hardiono, Hardiono; Kuntaman, Kuntaman; Wasito, Eddy Bagus; Lusida, Maria Inge
Folia Medica Indonesiana Vol. 54, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Carbapenemase-producing Escherichia coli (E. coli) has caused trouble in therapeutic antibiotic selection. Carbapenemase screening procedure in laboratories is usually based on inacurate semi-automatic system. Confirmation and classification of carbapenemases according to Ambler can be done with combination of phenotypic methods, i.e., Modified Hodge Test (MHT), Sodium Mercaptoacetic Acid (SMA), and 3-Aminophenylboronic Acid (PBA). This study aimed to compare profiles of carbapenemase-producing E. coli which were confirmed and classified phenotypically with the genotypic profiles. E. coli isolates from urine specimens which were potential as carbapenemase-producers according to semi-automatic system BD Phoenix were phenotypically tested with MHT, SMA, and PBA. Isolates were grouped as carbapenemase-producers and non carbapenemase-producers. Phenotypic carbapenemase-producer isolates were classified based on Ambler criteria. All isolates were then tested with Polymerase Chain Reaction (PCR) for the presence of OXA-48, IMP1, IMP2, GES, VIM, NDM, KPC genes. Out of 30 isolates, 6 isolates (20.0%) were MHT positive, and 25 isolates (83.3%) were SMA positive, which indicated that most isolates produced were carbapenemase Ambler B. PCR confirmed 12 isolates (40.0%) had VIM gene which were classified as carbapenemase Ambler B. Phenotypic confirmatory test had 100% sensitivity and 22.2% specificity. Classification with phenotypic confirmatory test had 91.7% match with PCR. Phenotypic confirmatory test detected more carbapenemase than PCR. This low specificity may be caused by inappropriate use of diagnostic gold standard. PCR should not be used for routine carbapenemase confirmation because of vast diversity of carbapenemases. Phenotypic confirmatory test can classify carbapenemase according to Ambler classification.
Effect of Glucose Induction on Biofilm Density in Clinical Isolate Acinetobacter baumannii Patients in Intensive Care Unit of Dr. Soetomo Hospital, Surabaya Lindarto, Wira W; Wasito, Eddy Bagus; Debora, Kartuti
Folia Medica Indonesiana Vol. 56, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

This study aimed to analyze the effect of glucose induction on the clinical isolate biofilm density of Acinetobacter baumannii. Thirteen clinical isolates of A. baumannii non biofilm forming were collected from non-DM patients who were treated at the ICU of Dr. Soetomo Hospital, Surabaya, was treated with the addition of 0.08% glucose, 0.15% glucose, 0.2% glucose, and 0.4% glucose in TSB growth media, followed by biofilm density examination with Tissue Culture Plate Method (TCPM) using 96 wells flatbottomed polyesterene tissue culture plate and read by autoreader ELISA with a wavelength of 630 nm (OD630). Biofilm density obtained was analyzed using ANOVA statistical analysis. The results of OD630 showed that the biofilm density increased significantly at the addition of 0.2% and 0.4% glucose. There was a significant increase in biofilm density at the addition of 0.2% and 0.4% glucose so that the management of blood sugar levels in ICU patients was needed before and when medical devices were installed.
Comparison between Exposure of Ciprofloxacin and Cefotaxime on Developing of Escherichia coli ESBL Amin, Muhammad; Wasito, Eddy Bagus; Triyono, Erwin Astha
Folia Medica Indonesiana Vol. 56, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

This study aimed to compare ciprofloxacin and cefotaxime exposure to develop ESBL producing Escherichia coli (E. coli). A total of 16 isolates of cefotaxime sensitive E. coli and ciprofloxacin were exposed to ciprofloxacin and cefotaxime for 14 days using the Kirby-Bauer antibiotic disc diffusion method. Colonies that grew on the edge of the inhibiting zone were exposed each day by the same method. Furthermore, we observed the occurrence of resistance to cefotaxime as ESBL screening test. Isolates were resistant, the following day the ESBL was confirmed by the Modified Double Disk Sinergy Test (MDDST) method using Cefotaxime (CTX), Ceftazidime (CAZ), Aztreonam (ATM), and Amoxilin Clavulanate (AMC) antibiotic discs. From 16 isolates of ESBL producing E. coli exposed to ciprofloxacin, it was obtained 4 (25%) to ESBL E. coli. ESBL production occurred after E. coli was exposed to ciprofloxacin on days 5, 6, 7, and 12. While those exposed to cefotaxime none becomes ESBL E. coli. There was no difference between ciprofloxacin and cefotaxime exposure to develop ESBL producing E. coli (p=0.101; Chi-square).
Co-Authors , Ivanna , Kuntaman Achmad Basori, Achmad Agung Dwi Wahyu Widodo Alimsardjono, Lindawati Ardhiya Puspita Arizandy, Radita Yuniar Asnia Zainuddin Bambang Hermanto Branandito Putra Budi Utomo Budiono Budiono Budiono Budiono Eko Budi Koendhori, Eko Budi Erwin Astha Triyono H.M. Soekry Erfan Kusuma Hanindita, Meta Herdiana Hardiani, Kartika Hardiono Hardiono Hardiono Hardiono, Hardiono I Nengah Wirajana I'tishom, Reny Ishak Samuel Wuwuti Isnaeni Isnaeni Ivanna Kartuti Debora Kartuti Debora, Kartuti Kawilarang, Arthur Pohan Kazuo Sakka Kimura, Tetsuya Kimura, Tetsuya Kuntaman Kuntaman Kusuma, Soekry Erfan Kusuma, Soekry Erfan Linda Dewanti Lindarto, Wira W Margaretha Nathania Maria Inge Lusida Maria Inge Lusida Muhammad Amin Nasronudin Nasronudin Neisya Intan Cahyaningtyas Agung Putri Ni Nyoman Tri Puspaningsih Nisa Amnifolia Niazta Nur Aisiyah Widjaja, Nur Aisiyah Nurina Hasanatuludhhiyah Nurwasis Nurwasis Nyilo Purnami Puspita, Ardhiya Radita Yuniar Arizandy Ramadhani Ramadhani Ratna Kusumawati Roedi Irawan S.Pd. M Kes I Ketut Sudiana . Sakka, Kazuo Sakka, Kazuo Silvia Sutandhio Soekry Erfan Kusuma SOETJIPTO . Suharyadi Sasmanto Tetsuya Kimura Triffit Imasari WINARTO Wiwiek Tyasningsih Wuwuti, Ishak Samuel Yulia Sari Ismail Zuhria, Ismi