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All Journal Jurnal Kimia (Journal of Chemistry) Jurnal Veteriner Jurnal Kedokteran Syiah Kuala Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya ANNALES BOGORIENSES Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Jurnal Natural Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Annales Bogorienses Folia Medica Indonesiana
Eddy Bagus Wasito
Departemen Mikrobiologi Kedokteran, Fakultas Kedokteran, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Proportions of Group B Streptococcus Isolation from Pregnant Women's Vaginal and Rectal Swab Specimens at a Tertiary Hospital in Surabaya, Indonesia , Ivanna; Wasito, Eddy Bagus; Debora, Kartuti
Folia Medica Indonesiana Vol. 59, No. 1
Publisher : Folia Medica Indonesiana

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Highlights: • Rectal and vaginal swab specimens were collected from pregnant women, and there was no significant difference in the proportions of group B Streptococcus isolation. • Combined vaginal and rectal swab cultures provide a higher isolation of group B Streptococcus. Abstract : Group B Streptococcus is a Gram-positive bacterium found in women. It causes high-risk mortality in pregnant women, newborns, and the elderly. This study aimed to compare group B Streptococcus (GBS/Streptococcus agalactiae) proportions from different collection sites (vaginal and rectal swabs). This was an analytic observational study with a hospital-based cross-sectional design. A total of 74 swabs were taken from 37 pregnant women at 35–37 weeks of gestation. Each participant provided a vaginal swab and a rectal swab, which were cultured in Todd Hewitt broth, blood agar, and CHROMagar. The specimens were subsequently identified using the VITEK 2 system. The GBS isolation percentages from the vaginal and rectal swab specimens were determined to be 13.5% and 8.1%, respectively. The McNemar test had a result of 0.697, and the Cohen's kappa test had a result of 0.165. To conclude, there was no significant difference in GBS isolation proportions between the vaginal and rectal swab cultures. Combined vaginal and rectal swab cultures were required to increase GBS isolation from pregnant women.
Risk Factors of Urinary Tract Infections Caused by Extended-Spectrum Î’-Lactamase-Producing Escherichia Coli in Inpatients at a Tertiary Hospital in Surabaya, Indonesia Wuwuti, Ishak Samuel; , Kuntaman; Wasito, Eddy Bagus
Folia Medica Indonesiana Vol. 58, No. 3
Publisher : Folia Medica Indonesiana

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Highlights: • ESBL frequency in women was higher than in men. • Catheter use in patients with urinary tract infections and recurrent urinary tract infections has four times the risk of ESBL caused by Escherichia coli bacteria • ESBL-producing E.coli bacteria are resistant to amoxicillin-clavulanic, ceftazidime, aztreonam, and ceftriaxone. Abstract: The incidence of urinary tract infections (UTIs) caused by extended-spectrum beta-lactamase (ESBL) producing Escherichia coli (E. coli) bacteria has become a global problem and has increased in recent years. The purpose of this study was to analyze the risk factors for the incidence of UTI in inpatients at Dr. Soetomo General Academic Hospital Surabaya. This research was an analytic descriptive study. Specimens were sent to the Clinical Microbiology Laboratory using sterile containers and processed according to standard laboratory procedures. It resulted in ESBL-producing E. coli that were used as a case group and non ESBL-producing E. coli as a control group. The identification and testing of antibiotic susceptibility were carried out using the BD Phoenixâ„¢ Automated Microbiology System. Ninety-four bacterial isolates were collected, consisting of 54 (57.4%) ESBL-producing E. coli bacteria and 40 (42.6%) non ESBL-producing E. coli bacteria. Recurrent UTIs (OR = 4.31; ρ =0.002; 95% CI = 1.68-11.04) and catheter use (ρ = 0.049; OR = 4.250; 95% CI = 1.050-17.210) were used as independent risk factors caused by ESBL-producing E. coli bacteria. Recurrent UTIs and catheter use were dependent risk factors caused by ESBL-producing E. coli bacteria.
The Carrier Rate of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Cockroaches (Periplaneta americana) in Hospital and Community Puspita, Ardhiya; Arizandy, Radita Yuniar; Wasito, Eddy Bagus; , Kuntaman
Folia Medica Indonesiana Vol. 57, No. 4
Publisher : Folia Medica Indonesiana

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Highlight:• Bacteriologically for colonization of ESBL producing Enterobacteriaceae in cockroaches (Periplaneta americana) were analyzed. • The prevalence of ESBL producing bacteria among cockroaches in hospitals is bigger than in households. Abstract: Cockroach (Periplaneta americana) is one of the vectors in the environment that can transmit disease. Cockroaches can act as potential mechanical vectors of antibiotic resistant bacteria. Enterobacteriaceae is a gram-negative bacteria that has natural habitats in the digestive tract of humans and animals. Enterobacteriaceae that produce Extended Spectrum β-lactamases (ESBLs) have emerged as major pathogens in hospitals. The study analyzed the prevalence of ESBL producing bacteria in cockroaches that lived in hospitals and residential homes. In this study, a total of 200 cockroaches consisting of 100 cockroaches from the hospital environment and 100 cockroaches from the residential environment were analyzed bacteriologically for colonization of ESBL producing Enterobacteriaceae. The specimen of the alimentary tract was taken and sub-cultured in MacConkey agar supplemented with cefotaxime 2 ug/ml. Growth colonies were suggested as an ESBL-producing bacteria, then were confirmed as ESBL producers by the Double Disk Synergy Test (DDST). The ESBL gene was detected by Polymerase Chain Reaction (PCR). Among 100 household cockroach samples, 14 (14%) were identified as ESBL producers, while 100 hospital cockroaches were 26 (26%) positive ESBL. The ESBL gene, in hospital cockroach were identified of CTXM 19 (19%), SHV 7 (7%), and not any TEM gene, while among household cockroaches were identified CTXM 2 (2%), SHV 11 (11%), and also not detected TEM ESBL gene. Among ESBL genes, only the CTXM gene was significantly different between household and hospital cockroaches.
Co-Authors , Ivanna , Kuntaman Achmad Basori, Achmad Agung Dwi Wahyu Widodo Alimsardjono, Lindawati Ardhiya Puspita Arizandy, Radita Yuniar Asnia Zainuddin Bambang Hermanto Branandito Putra Budi Utomo Budiono Budiono Budiono Budiono Eko Budi Koendhori, Eko Budi Erwin Astha Triyono H.M. Soekry Erfan Kusuma Hanindita, Meta Herdiana Hardiani, Kartika Hardiono Hardiono Hardiono Hardiono, Hardiono I Nengah Wirajana I'tishom, Reny Ishak Samuel Wuwuti Isnaeni Isnaeni Ivanna Kartuti Debora Kartuti Debora, Kartuti Kawilarang, Arthur Pohan Kazuo Sakka Kimura, Tetsuya Kimura, Tetsuya Kuntaman Kuntaman Kusuma, Soekry Erfan Kusuma, Soekry Erfan Linda Dewanti Lindarto, Wira W Margaretha Nathania Maria Inge Lusida Maria Inge Lusida Muhammad Amin Nasronudin Nasronudin Neisya Intan Cahyaningtyas Agung Putri Ni Nyoman Tri Puspaningsih Nisa Amnifolia Niazta Nur Aisiyah Widjaja, Nur Aisiyah Nurina Hasanatuludhhiyah Nurwasis Nurwasis Nyilo Purnami Puspita, Ardhiya Radita Yuniar Arizandy Ramadhani Ramadhani Ratna Kusumawati Roedi Irawan S.Pd. M Kes I Ketut Sudiana . Sakka, Kazuo Sakka, Kazuo Silvia Sutandhio Soekry Erfan Kusuma SOETJIPTO . Suharyadi Sasmanto Tetsuya Kimura Triffit Imasari WINARTO Wiwiek Tyasningsih Wuwuti, Ishak Samuel Yulia Sari Ismail Zuhria, Ismi