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Effectiveness Meniran ( Phyllanthus Niruri Linn ) as Immunomodulation onLymphocyte of Broiler Infected with Enterotoxin Escherichia Coli Resistant Antibiotics Wahjuni, Retno Sri; Sabdoningrum, Emy Koestanti; Hidanah, Sri
Jurnal Vokasi Indonesia Vol. 5, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Enterotoxin Escherichia coli resistant to antibiotics on broiler. The used of antibiotics should be re- evaluated, as well as over Escherichia coli enterotoxin. Plants meniran (Phyllanthus niruri Linn) is a plant that can be used as an alternative prevention and treatment of diseases caused by Escherichia coli enterotoxin. In this research, broilers at the age of 23 days were divided into six groups. Two groups are positive control was infected by Escherichia coli and negative control was not infected by Escherichia coli. Three groups are extract of Phyllanthus niruri Linn. plant that variety in three dosage: 20%, 25%, 30% and antibiotic. After five days treatment the broiler was to take the sample of blood tissue. The data were tested with Annova and continued by Duncan multiple range test. There was a significant difference of immunomodulation activity between treatment group doses 30% has been able to decrease lymphocyte on blood. The result showed doses 30% can be used as immunomodulation to Escherichia coli.
Potensi Bakteri Lactococcus sp. dan Lactobacillus sp. untuk Peningkatan Kualitas Limbah Kulit Kacang Sebagai Alternatif Bahan Pakan [Potential of Lactococcus sp. and Lactobacillus sp. Bacteria for Quality Improvement of Peanut Peel Waste as Alternative Feed Ingredients] Widya Paramita Lokapirnasari; Oky Setyo Widodo; Emy Koestanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 10 No. 1 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v10i1.8547

Abstract

                                                    AbstrakTujuan penelitian ini untuk menterminasi peningkatan kandungan nutrien dalam fermentasi makanan yang terdapat dari kulit kacang sebagai bahan pakan alternatif untuk ternak dan ikan. Penelitian ini terdiri dari tiga perlakuan dan lima replikasi P0: kulit kacang+isolat 0%; P1: kulit kacang+1% Lactococus sp. ; P2 : kulit kacang+1% Lactobacillus sp. Proses fermentasi dilakukan selama 5 hari dalam keadaan aerob. Hasil menunjukan bahwa terdapat berdedaan yang signifikan antara perlakuan (p<0,05). Penggunaan isolat Lactococcus sp. dan Lactobacillus sp. dapat meningkatkan kandungan nutrient dari limbah kulit kanang. Terdapat penurunan aerob mentah dari 3,80 sampai 7,70% terdapat peningkatan elestrak nitrogen bebas dari 3,925 sampai 4,38% terdapat peningkatan kandungan energi pencernaan 7.13% sampai 9.30%. berdasarkan hasil penelitian dapat disimpulkan bahwa pengguna 1% Lactococcus sp. dan 1% Lactococcus sp. dapat meningkatkan nilai nutrisi di limbah kulit kacang yang dapat digunakan sebagai alternatif bahan pakan ternak dan ikan.                                                   AbstractThis study aims to determine the increasing of nutrient content in the fermentation of feed stuff derived from peanut wastes as an alternative feed material for livestock and fish. The study consisted of three treatments and five replications, namely P0: peanut peel + 0% isolate, P1: peanut peel + 1% Lactococcus sp, P2: peanut peel +1% Lactobacillus sp. The fermentation process was carried out for 5 days under a facultative anaerobic condition. The results showed that there was a significant difference between treatments (p <0.05). The use of Lactococcus sp and Lactobacillus sp isolates can increase the nutritional content of peanut peel wastes. There was a decrease in crude fiber content (CF) of 3.80 to 7.70%, there was an increase of Nitrogen free extract (NFE) of 3.925 to 4.38%, there is an increase in digestible energy (DE) content of 7.13% to 9.30%, there was the increasing metabolizable energy (ME) of 7.13% to 9.12% compared to control. Based on the results it can be concluded that the using of 1% Lactococcus sp and 1% Lactobacillus sp. can increase nutritional value of peanut peel waste that can be used as an alternative ingredients of animal and fish.
New Biotype of Vibrio cholerae O1 from Clinical Isolates in Surabaya de Vries, Garry Cores; Sabdoningrum, Emy Koestanti; Rahardjo, Dadik
Indonesian Journal of Tropical and Infectious Disease Vol. 1 No. 1 (2010)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.647 KB) | DOI: 10.20473/ijtid.v1i1.3724

Abstract

A surveillance of new pathogenic variants of Vibrio cholerae O1 strains was initiated to identify the emerge and spread throughout Surabaya. Findings from seven years (1994–2000) and from years 2008 until now by using a two-fold surveillance strategy was pursued involving 1) hospital-based case recognition, and 2) environment samples. Rectal swabs and environment samples were transported to ITD-UNAIR, Surabaya for culture and isolates were characterized by serotypic identification and arbitrarily primed PCR fingerprints revealed a group of strains with similar fingerprint patterns that are distinct from those of the current El Tor epidemic strain. These strains have been analyzed by in vitro technique and the group has been denominated the Surabaya-Indonesian variant of V. cholerae O1.
EFFECTIVENESS OF MENIRAN (PHYLLANTHUS NIRURI LINN) AS ANTIBACTERIAL FOR RESISTANCE ANTIBIOTICS PREVENTION OF ENTEROTOXIN ESCHERICHIA COLI Hidanah, Sri; Sabdoningrum, Emy Koestanti; Wahyuni, Retno Sri; Dewi, Arini Rahmi; Safitri, Erma -
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 2 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.153 KB) | DOI: 10.20473/ijtid.v7i2.7328

Abstract

Escherichia coli (E. coli) can be isolated from the environment both inside and outside the hospes body. There were 89 serotypes in which showed 21% resistance to various antibiotics, such as enterotoxigenic E.coli. Alternative efforts are needed to overcome this, one of them through the use of medicinal plants, such as meniran (Phyllanthus niruri Linn). Meniran plant is an immunomodulator that serves to repair the immune system of the body. The aim of research is the research was done through several stages: isolation and identification of enterotoxigenic E. coli from several broiler farms in East Java using the polymerase chain reaction (PCR) method, E. coli resistance test against some antibiotics, making meniran extract and activation test against enterotoxigenic E. coli n The study was divided into five treatments: T0+ (group of chickens were infected by enterotoxigenic E. coli T0- (control group, not infected), T1 (infected by enteroxigenic E. coli + 20% meniran extract), T2 (infected by enterotoxigenic E. coli + 25% extract meniran), T3 (infected by enterotoxigenic E. coli + 30% extract meniran). Data were analyzed by ANOVA (Analysis of Variance). The results show that all of E. coli DNA isolates which tested by the PCR method show positive reactions at 600 bp. The next stage, the enterotoxigenic E. coli are resistant to some antibiotics, such as amoxicillin, ampicillin, erythromycin, cephalosporins, tetracycline, cloxacillin and gentamicin. Furthermore, the 30% Phyllanthus niruri Linn extract is effective as an antibacterial for antibiotic resistance enterotoxigenic E. coli. next necessary to write that: The 30% meniran extract is potential for kill of enterotoxigenic E. coli
Effectiveness of Phyllanthus niruri and Andrographis paniculata Extracts on Egg Quality in Laying Hens with Avian Pathogenic Escherichia coli Hidanah, Sri; Sabdoningrum, Emy Koestanti; Chusniati, Sri; Nurliyani, Nurliyani; Khairullah, Aswin Rafif; Nayan, Nazri
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.48-54

Abstract

Providing sufficient and high-quality eggs plays an essential role in accommodating food safety and food security for consumers. The purpose of this study was to determine the effect of Phyllanthus niruri and Andrographis paniculata extracts on egg yolk index and haugh units (HU) value in laying hens with Avian Pathogenic Escherichia coli. This type of study was experimental using a complete randomized design with 100 experimental units of five treatments (P-) placebo, (P+) infected with Avian Pathogenic E. coli without P. niruri and A. paniculata extract, then groups of chickens infected with Avian Pathogenic E. coli and administration of (P1) 10% of P. niruri and 30% of A. paniculata, (P2) 20% of P. niruri and 20% of A. paniculata, (P3) 30% of P. niruri and 10% of A. paniculata then egg yolk index and HU value were evaluated. The data obtained were analyzed using ANOVA followed by Duncan's test. The results showed a significant difference between the treatment groups based on the egg yolk index and the HU value. It can be concluded that P2 can be recommended for laying hens with avian pathogenic E. coli.
PEMBERIAN EKSTRAK MENIRAN (Phyllanthus niruri Linn) TERHADAP GAMBARAN HISTOPATOLOGI INFUNDIBULUM AYAM PETELUR YANG DIINFEKSI Escherichia coli Irawan, Adinda Riezki; Sabdoningrum, Emy Koestanti; Hidanah, Sri; Chusniati, Sri; Madyawati, Sri Pantja; Tehupuring, Benjamin Christoffel
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19992

Abstract

The purpose of this research aimed to prove the effect of meniran extract (Phyllanthus niruri Linn) on layer hen infected with Escherichia coli to reduce inflammation and congestion on infundibulum histopathological view.  This research used 20 samples of layer hen. The treatment in this research was P0- (not infected with Esherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P0+ (infected with Escherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P1 (infected with Escherichia coli and treated with 10% meniran extract (Phyllanthus niruri Linn)), P2 (infected with Escherichia coli and treated with 20% meniran extract (Phyllanthus niruri Linn)), P3 (infected with Escherichia coli and treated with 30% meniran extract (Phyllanthus niruri Linn)). The data of this study analyzed by Kruskal-wallis and followed with Mann-withney test. The result of this research can be concluded that 10% concentration of meniran extract (Phyllanthus niruri Linn) was effective to eliminate Escherichia coli, 20% concentration of meniran extract (Phyllanthus niruri Linn) able to promote antibacterial activity, and 30% concentration of meniran extract shown antibacterial rate on high potential so it can be used to reduce inflammation and congestion as the result of Escherichia coli infection.
Pengaruh Probiotik Bakteri Asam Laktat dalam Air Minum Terhadap Konversi Pakan Ayam Broiler Azizah, Nanda Kholifatul; Sarmanu, Sarmanu; Utomo, Budi; Sabdoningrum, Emy Koestanti; Lokapirnasari, Widya Paramitha; Supranianondo, Koesnoto
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28587

Abstract

This study aimed to determine the effect of probiotics of lactic acid bacteria in drinking water on feed consumption, weight gain and feed conversion ratio of broiler chickens. This research is an experimental research. The experimental animals used in this study were 28 male broiler chickens strain Cobb 700 aged 20 days. P0 was not given the addition of probiotic lactic acid bacteria, P1 was given the addition of probiotic lactic acid bacteria Lactobacillus acidophilus in 1ml/liter drinking water, P2 was given the addition of probiotic lactic acid bacteria Lactobacillus casei in drinking water 1ml/liter, P3 was given the addition of probiotic lactic acid bacteria Lactobacillus fermentum in drinking water 1ml/liter, P4 was given the addition of probiotic lactic acid bacteria Lactobacillus plantarum in drinking water 1ml/liter, P5 was given the addition of probiotic lactic acid bacteria Pediococcus in drinking water 1ml/liter, P6 was given the addition of probiotic mix lactic acid bacteria in drinking water 1ml /liter. Collecting data for the last 1 week by weighing the remaining consumption of feed given and weight gain of broiler chickens. The data obtained were analyzed using the Analysis of Variant (ANOVA) statistical method and continued with Duncan. Based on the results of the calculation of the amount of feed consumption and weight gain in broiler chickens, it showed that the administration of probiotic lactic acid bacteria 1ml/liter of water in broiler chickens could increase body weight gain, reduce feed conversion rates but did not reduce broiler feed consumption (p<0.05).
Effect of Turmeric Flour (Curcuma domestica) and Fish Oil on Daily Egg Production and Organoleptics of Quail Eggs (Coturnix-coturnix japonica) Lovela, Aprinda Ratna; Al-Arief, Moh. Anam; Ismudiono, Ismudiono; Supranianondo, Kusnoto; Lokapirnasari, Widya Paramita; Sabdoningrum, Emy Koestanti
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28591

Abstract

The purpose of this study was to determine the effect of adding the concentration of turmeric flour and fish oil to the quail formula feed. This research is an experimental study using the factorial method. This experiment used 96 quail (Cortunix cortunix japonica), which were randomized into 6 treatments with 4 replications. Quails were divided into 6 groups and treated with different concentrations of turmeric flour and fish oil in each feed formula. The effects of different formula feeds were observed on changes in egg production and organoleptic changes. Egg production data were taken every day from week 3 to week 4 during the treatment period. The data taken is called Quail Day Production (QDP%). The organoleptic data of eggs were taken at the 3rd week of treatment and the preference test was carried out by 40 panelists on the aroma, taste and level of preference of quail eggs after being boiled. The addition of 0.3% turmeric flour significantly affected egg production but its combination with fish oil did not affect egg production. The combination of the concentration of turmeric flour and fish oil does not change the aroma of the eggs, but changes the taste of the eggs.
Penerapan Teknologi Nano Ekstrak Meniran pada Pakan dan Manajemen Pemeliharaan untuk Meningkatkan Kemandirian Ekonomi Kelompok Ternak Kambing dan Domba di Desa Sepande, Sidoarjo Hidanah, Sri; Sabdoningrum, Emy Koestanti; Yudaniayanti, Ira Sari; Fitriyah, Hadiah; Miarsono, Sigit; Warsito, Sunaryo Hadi; Lamid, Mirni; Lokapirnasari, Widya Paramita; Arif, Mohammad Anam Al; Rosyada, Zulfi Nur Amrina
Lumbung Inovasi: Jurnal Pengabdian kepada Masyarakat Vol. 9 No. 4 (2024): December
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/linov.v9i4.2201

Abstract

Tujuan dari program ini adalah meningkatkan produktivitas ternak kambing dan domba melalui penerapan teknologi complete feed dengan penambahan feed additive nanopartikel ekstrak meniran (Phyllanthus niruri). Masalah utama yang dihadapi oleh peternak di Desa Sepande adalah rendahnya produktivitas ternak akibat pengelolaan pakan yang masih tradisional dan kurangnya pemahaman tentang ransum pakan yang tepat. Metode yang digunakan dalam program ini meliputi penyuluhan, pelatihan, dan evaluasi dengan menggunakan teknologi complete feed berbasis nanopartikel. Hasil yang diperoleh menunjukkan adanya peningkatan bobot harian ternak kambing dan domba yang lebih spesifik, yaitu dari 0,3 kg/hari menjadi 0,5 kg/hari, yang menunjukkan kenaikan sebesar 66,7%. Evaluasi post-test juga mengungkapkan bahwa 90% peternak menyatakan akan melanjutkan penggunaan teknologi ini. Peningkatan produktivitas ternak berdampak langsung pada peningkatan pendapatan peternak hingga 30%. Program ini berkontribusi terhadap pencapaian Tujuan Pembangunan Berkelanjutan (SDGs), khususnya SDG 2 (Tanpa Kelaparan) dan SDG 8 (Pekerjaan Layak dan Pertumbuhan Ekonomi), dengan mendukung ketahanan pangan dan pertumbuhan ekonomi yang inklusif di sektor peternakan. Dengan adopsi teknologi ini, peternak mampu meningkatkan pendapatan mereka secara signifikan. Application of Nano Meniran Extract Technology in Feed and Management Practices to Enhance Economic Independence of Goat and Sheep Farmers in Sepande Village, Sidoarjo Abstract The purpose of this program is to increase the productivity of goats and sheep through the application of complete feed technology with the addition of nanoparticle feed additive of meniran extract (Phyllanthus niruri). The main problem faced by farmers in Sepande Village is the low productivity of livestock due to traditional feed management and lack of understanding of the right feed ration. The methods used in this program include counseling, training, and evaluation using nanoparticle-based complete feed technology. The results obtained showed a more specific increase in the daily weight of goats and sheep, from 0.3 kg/day to 0.5 kg/day, which showed an increase of 66.7%. The post-test evaluation also revealed that 90% of farmers stated that they would continue using this technology. The increase in livestock productivity has a direct impact on increasing farmers' income by up to 30%. This program contributes to the achievement of the Sustainable Development Goals (SDGs), specifically SDG 2 (No Hunger) and SDG 8 (Decent Work and Economic Growth), by supporting food security and inclusive economic growth in the livestock sector. With the adoption of this technology, farmers are able to significantly increase their income.
White Kupang Flour (Corbula faba hinds), Fish Meal and Coconut Oil Usage on the Broilers Carcass Percentage and Abdominal Fat Virgy Dzakina Fahrezy; Mirni Lamid; Setiawan Koesdarto; Emy Koestanti Sabdoningrum; Widya Paramita Lokapirnasari; Sunaryo Hadi Warsito
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51393

Abstract

This study was conducted to determine the effect of using white mussel flour fish flour and coconut oil on carcass percentage and abdominal fat percentage in broilers. This study used a completely randomized design consist 4 treatments and 5 replications. The study used DOC ( Day Old Chick ) with the strain Lohman MB 202 Platinum for 20 animals . The treatment in this study was ration without using white mussel flour (P0), ration using 10% white mussel flour (P1), ration using 12.5% white mussel flour (P2), ration using 15% white mussel flour (P3). Variables observed were carcass percentage and abdominal fat percentage. Data were analyzed by Analysis of Variance (ANOVA) if there is a significant effect then proceed with Duncan's Multiple range test significance level of 5%. The results of the analysis showed that P3 showed a significant difference with the control treatment (P0) and treatment P1 (p<0.05). The best broiler carcass percentage was in treatment P3 (76.02%) and P2 (74.7%) while the lowest was in treatment P0 (71.20%). The results of Duncan's test on the percentage of abdominal fat showed that the control treatment (P0), treatment P1, treatment P2, and treatment P3 were not significantly different (p> 0.05).
Co-Authors Agustono Agustono Ahmad Fadlur Rahman Bayuny Al arif, Mohammad Anam Al-Arief, Moh. Anam Al-Arif , M. Anam Al-Arif, Anam Anam Al-Arif Anita Nur Aida Aida Anwar Ma'ruf Astuti, Elita Sriwidya Azizah, Nanda Kholifatul Bayu Dwi Saputra Benjamin Christoffel Tehupuring, Benjamin Christoffel Budi Utomo Candika, Yossy Imam Chusniati, Sri Dadik Rahardjo, Dadik de Vries, Garry Cores Dewi, Arini Rahmi Dian Ayu Permatasari Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Elita Triwijayanti Aqfari Endang Suprihati Epy Muhammad Luqman Erma Safitri Fadila Zikri Amanda Fatin Fadhilah Hasib Febriano, Muhammad Galang Hadiah Fitriyah Hana Eliyani Hana Eliyani Hasib, Fatin Hendarti, Gracia Angelina Hernanda, Ary Setya Herry Agoes Hermadi Heru Pramono Hidanah, Sri Hidayatik, Nanik Ira Sari Yudaniayanti Irawan, Adinda Riezki Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Koesnoto Supranianondo Kurnijasanti, Rahma Larasati, Anissa Dhea Lilik Maslachah Lina Wafia Asmi Lokapirnasari, Widya Paramitha Lovela, Aprinda Ratna Mafruchati, Maslichah Melanie Aulia Ashfiyah Miarsono, Sigit Mirni Lamid Mirni Lamid Moh. Zaky ‘Ubaidillah Muchammad Yunus Mufasirin Mustofa Helmi Effendi Myrna Budi Nayan, Nazri Nidom, Chairul Anwar Nove Hidajati Novianti, Arindita Niatazya Nunuk Dyah Retno Lastuti Nurliyani Nurliyani Poedji Hastutiek Pratama Putra Banusiandana Pudji Srianto Rahmahani, Jola Rahmaniar Larasati Rahmawati, Kadek Rara Irbah Fitriana Karimah Revina Ayu Septiani Rochmah Kurnijasanti Romziah S. Rosyada, Zulfi Nur Amrina Safitri, Erma - Sarmanu, Sarmanu Setiawan Koesdarto Setyantini, Woro Hastuti Siti Eliana Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Hidanah Sri Mulyati Sri Pantja Madyawati Sugiyono Suherni Susilowati Supranianondo, Koesnoto Supranianondo, Kusnoto Suwarno Suwarno Suwarno Swari, Wahyu Retno Tacharina, Martia Rani Tatik Hernawati Tri Bhawono D. Tri Wahyu Suprayogi Virgy Dzakina Fahrezy Wahjuni, Retno Sri Wahyuni, Retno Sri Warsito, Sunaryo Hadi Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Yudanianyi, Ira Sari Yudaniayanti, Ira Yulianna Puspitasari ZULFAH