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Detection of Mycotoxigenic Fungi on Food from Markets with the Polymerase Chain Reaction Method for Halal and Thoyiban Food Mohammad Sukmanadi; Retno Sri Wahjuni; Kadek Rachmawati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14813

Abstract

Secondary metabolites of filamentous mold are mycotoxins, which in some situations can develop on foodsderived from plants or from animals. Fusarium, Aspergillus, and Penicillium are the most common types ofmold that produce mycotoxins and also often contaminate human food and animal feed. Aflatoxins includingaflatoxin B1, B2, G1 and G2 are produced by A. flavus and A. parasiticus. M1 and M2 aflatoxins arefound in dairy products. In this study, we used PCR to detect and identify mycotoxigenic fungi material infoods from traditional markets and supermarkets in Surabaya, Indonesia. Samples of chicken meat from atraditional market and from a supermarket (10 pieces each) were placed in a conical tube and crushed in PBS.The crushed samples were centrifuged and the supernatants were cultured on potato dextrose agar (PDA)media and observed using a reverse microscope. DNA was isolated from cultured samples and subjectedto PCR with primers specific for genes encoding aflatoxins. Interestingly, we revealed that the PolymeraseChain Reaction (PCR) analysis showed A. flavus and A. ochraceus were present on chicken meat sold attraditional markets and supermarkets. In sum, enhanced precautions may be needed to ensure that foods soldin traditional markets and supermarkets are free from molds that have the potential to produce mycotoxins.Further studies are needed to detect and identify the prevalence of mycotoxins in the food supply.
PERAN GEN HEMAGLUTININ VIRUS AVIAN INFLUENZA H5N1 Moch Juddy Widjadja; Kadek Rachmawati; Muhammad Farid Rizal; Dini Fadhila Samjaya
Prosiding Seminar Nasional Pakar Prosiding Seminar Nasional Pakar 2019 Buku I
Publisher : Lembaga Penelitian Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pakar.v0i0.4223

Abstract

Dengan adanya kejadian luar biasa yang pertama virus avian influenza H5N1 tahun 1997, semakin jelas bahwa potensi virulensi virus H5N1 telah meluas ke manusia. Review ini disusun untuk memahami karakteristik virus, siklus replikasi virus, mekanisme virus masuk ke dalam hospes, peran hemaglutinin sebagai determinan patogenisitas, urutan basa hemaglutinin yang berperan dalam memicu peningkatan virulensi dan fungsi dari 6 segmen gen lainnya pada virus avian influenza. Review juga dibuat untuk memahami gambaran gen Hemaglutinin avian influenza virus H5N1 yang bertujuan untuk mengetahui penularan secara efisien dan replikasi virus avian influenza pada manusia, sehingga penularan selanjutnya dapat diantisipasi dengan baik. Kerja sama lintas sektor antara kementerian kesehatan, kementerian koordinator kesejateraan rakyat, kementerian lain, universitas dan organisasi yang berkompeten sangat dibutuhkan untuk mendukung pencegahan penyebaran virus avian influenza H5N1 di Indonesia.
POTENTIAL OPTIMIZATION OF TOBACCO AGRICULTURE WASTE IN WATUBONANG VILLAGE, BADEGAN DISTRICT, PONOROGO Rochmah Kurnijasanti; Sri Agus Sudjarwo; Kadek Rachmawati
Jurnal Layanan Masyarakat Vol. 7 No. 2 (2023): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v7i2.2023.245-251

Abstract

The aims of this community dedication: 1) Improve the economy of the villagers of Watubonang and Biting, Badegan Ponorogo district through changing mindsets to improve people’s welfare 2) Increasing the knowledge and skills of the villagers of Watubonang and Biting, Badegan Ponorogo district through technology transfer for making pecticide formulations, antiscabies drugs, antiseptik, compost manure disinfectant, dipping solution based on tobacco waste. The result of the communiy dedication implementation showed an increase in participants on how to manufacture various kinds of tobacco waste products from 6.7% to 93.3% and an increase in the skills of participants on how to manufacture various kinds of tobacco waste products from 6.7% to 93.3%. in addition to the increased knwoledge and skills of the community dedication participants in Watubonang and Biting villages, Badegan Ponorogo district, it showed a decrease in the average cost of using medicines, antiseptiks, and disinfectants per house from 210 thousand rupiah to 42 thousand rupiah. Form the results of community dedication in the villages of Biting and Watubonang, Badegan Ponorogo district, it is hoped that they will become fostered villages of FKH Unair.
Porang (Amorphophallus oncophyllus) tuber extract improved the histopathological features of diabetic rat (Rattus norvegicus) testicles Dwi Puspa Sukma Viranda; Rochmah Kurnijasanti; Kadek Rachmawati; Iwan Sahrial Hamid; Pudji Srianto; Hani Plumeriastuti; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.47-57

Abstract

This study aims to determine the effect of porang (Amorphophallus oncophyllus) tuber extract on the histopathological features of the testicle of alloxan induced rats (Rattus norvegicus). Thirty male rats were randomly divided into six groups. Rats in group C- were injected with distilled water, while rats in groups C+, T0, T1, T2, and T3 were injected with 120 mg/kg bw alloxan. Blood glucose was measured three days after alloxan injection. Rats in groups C- and C+ were then administered with 1% Na-CMC, whereas rats in groups T0, T1, T2, and T3 were then administered with 45mg/kg bw metformin, and 100, 200, and 400 mg/kg bw of porang tuber extract, respectively. All solution were given orally once every day for 14 days. Spermatogenic activity was assessed using the Johnsen scoring system and analyzed by Kruskal-Wallis, followed by the Mann-Whitney test. The diameter and epithelial thickness of the seminiferous tubule were measured using image raster software and analyzed using Anova followed by Duncan's test. The results showed that spermatogenesis score, diameter, and epithelial thickness of seminiferous tubule of group C+ were smaller (p <0.05) than group C-. Spermatogenesis scores of groups T0, T1, T2, and T3 were higher (p <0.05) than group C+. Seminiferous tubule diameter and epithelial thickness in groups T0, T1, and T2 were greater (p <0.05) than group C+. It could be concluded that porang tuber extract at a dose of 200 mg/kg bw improved the spermatogenesis score, diameter seminiferous tubule, and thickness of the epithelium of diabetic rats.
The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Mulberry (Morus alba L.) leaf extract enhanced spermatozoa motility, viability, and plasma membrane integrity of rats (Rattus norvegicus) exposed to e-cigarette smoke Ramadhani, Fifi Fauziah; Yulianna Puspitasari; Hermin Ratnani; Budi Utomo; Rochmah Kurnijasanti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.120-128

Abstract

This study investigates the impact of mulberry leaf extract on the viability, plasma membrane integrity, and motility of spermatozoa from male white rats (Rattus norvegicus) exposed to e-cigarette smoke. A total of twenty-five male rats were divided into five groups: negative control (NC), positive control (PC), T1, T2, and T3. All groups, except for the NC group, were exposed to e-cigarette smoke. Rats in the T1, T2, and T3 groups received mulberry leaf extract in doses of 100, 200, and 400 mg/kg bw, respectively, while the NC and PC groups were given a placebo of 1% Na-CMC. Both the mulberry leaf extract and the placebo were administered daily, beginning three days prior to the start of e-cigarette smoke exposure, which lasted for 28 days. Results showed that spermatozoa motility, plasma membrane integrity, and viability in the experimental groups were significantly lower than those in the NC group (p <0.05). Conversely, rats in the T1, T2, and T3 groups that received mulberry leaf extract demonstrated significantly greater spermatozoa viability, plasma membrane integrity, and motility compared to the PC group (p <0.05). The T3 group exhibited the most pronounced improvements, with significantly enhanced spermatozoa viability, membrane integrity, and motility (p <0.05) relative to the PC group. These results indicate that mulberry (Morus alba L.) leaf extract enhanced spermatozoa viability, plasma membrane integrity, and motility in white rats (Rattus norvegicus) subjected to e-cigarette smoke.
Meniran (Phyllanthus niruri Linn) Extract Potential on Texel Sheep Feed Consumption, Body Weight Gain and Feed Conversion Rara Irbah Fitriana Karimah; Sri Hidanah; Mohammad Anam Al-Arif; Kadek Rachmawati; Emy Koestanti Sabdoningrum; Suwarno
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.57619

Abstract

Meniran contains phytochemical compounds that are efficacious for productivity. This research was conducted to determine the effectiveness of meniran extract on feed consumption, body weight gain and feed conversion ratio of Texel sheep. This research was conducted using Completely Randomized Design by divided 20 Texel sheeps into 4 treatments, each treatment consisted of 5 repetitions. The treatment groups consisted of : P0 (sheeps were given CMC-Na 0.5% solution), P1 (given meniran extracts at 5% concentration), P2 (given meniran extracts at 10% concentration) and P3 (given meniran extracts at 20% concentration). administration of treatment between groups using a syringe (20 ml) amount of 1ml/kgBB per oral. Data in the form of feed consumption, body weight gain and feed conversion ratio were then analyzed using ANOVA and continued with Duncan's test. The best result on body weight gain and feed conversion ratio by P1. This research show that meniran extracts had no effect on feed consumption but can increase body weight gain at 5% concentrations and decrease feed conversion ratio at 5% concentrations.
Survey of types and use of traditional medicinal plants for beef cattle in Bontang City Amar Ma'ruf Fachrul Mu'minin; Lilik Maslachah; Sri Hidanah; Ratna Damayanti; Kadek Rachmawati; Mohammad Sukmanadi
Jurnal Agro Veteriner Vol. 8 No. 1 (2024): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i1.64448

Abstract

Bontang city is suitable for research due to the limited data on the use of traditional herbal medicine in beef cattle. This study aims to survey the types and usage of traditional herbal plants in beef cattle in Bontang city, providing new insights for local farmers. Data collection involved observation, recording, and photographic documentation. Samples were taken from Bontang city, including West Bontang, South Bontang, and North Bontang districts. Traditional medicinal plants such as garlic, brotowali, coconut, turmeric, temulawak, guava, and lime thrive in these conditions. The majority of respondents are beef cattle farmers aged 51-60 years with a low level of education. Experience in cattle farming for more than 4 years has improved their skills and productivity. The primary source of information on herbal plants is passed down through generations from their parents. The results of the survey on the types and usage of traditional herbal plants in beef cattle in Bontang city can be summarized as follows. Seven plant species are used by beef cattle farmers as natural remedies, namely garlic, brotowali, coconut, turmeric, temulawak, guava, and lime. 
Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

Abstract

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.
Potential of meniran extract (Phyllanthus niruri Linn) on the economic value of feed and income over feed cost in Texel sheep Febriano, Muhammad Galang; Emy Koestanti Sabdoningrum; Pudji Srianto; Sri Hidanah; Soeharsono, Soeharsono; Kadek Rachmawati
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.70121

Abstract

The research was conducted using completely randomized design by divided 24 Texel sheeps into 4 treatments, each treatment consisted of 6 repetitions. The treatment groups consisted of: P0 (sheeps were given CMC-Na 0,5% solution), P1 (given meniran extracts at 5% concentration), P2 (given meniran extracts at 10% concentration) and P3 (given meniran extracts at 20% concentration). Administration of treatment between groups using a syringe (20 ml). The data were analyzed statistically using the Analysis of Variance (ANOVA) and continued with Duncan's test. The economic value of feed is obtained from the feed conversion value multiplied by the feed price, where the feed conversion is obtained from the ratio between the amount of feed consumption and body weight gain. The income over feed cost obtained through the difference between income and total feed costs, where the total feed price is obtained from the cost of Texel sheep feed plus the cost of meniran extract (Phyllanthus niruri Linn). P1 with meniran extract 5% gives the best result. It can be conclude that the addition of 5% meniran extract could reduce the economic value of feed and increase income over feed costs on Texel sheeps.