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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Research Journal of Life Science MNJ (Malang Neurology Journal) Paediatrica Indonesiana Majalah Kesehatan FKUB Medical Laboratory Technology Journal Jurnal Ilmiah Bidan Indonesian Journal of Medicine International Journal of Community Service Learning Jurnal Riset Kesehatan Poltekkes Depkes Bandung Journal of Issues in Midwifery Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Indonesian Archives of Biomedical Research (InABR) Journal of Global Pharma Technology Media Penelitian dan Pengembangan Kesehatan

Agustina Tri Endharti

Departemen Parasitologi, Fakultas Kedokteran Universitas Brawijaya Malang, Indonesia

Author-ID : 259783

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Membangun Desa Binaan Tanggap COVID-19, Lupus, Reumatik, dan Alergi: Upaya Menurunkan Angka Kejadian dan Mencegah Kekambuhan di Malang Handono, Kusworini; Wahono, Cesarius Singgih; Barlianto, Wisnu; Dewi, Elvira Sari; Sari, Tita Luthfia; Hasanah, Dian; Rahman, Perdana Aditya; Anshory, Muhammad; Wulandari, Desy; Sari, Dewi Purnama; Endharti, Agustina Tri; Nurdiana, Nurdiana; Kalsum, Umi; Susianti, Hani; Kalim, Handono
International Journal of Community Service Learning Vol 5, No 1 (2021): February 2021
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v5i1.30161

Kurangnya pengetahuan masyarakat tentang COVID-19, lupus, reumatik, dan alergi mempengaruhi keberhasilan terapi, penurunan angka kejadian, dan pencegahan kekambuhan.Â Tujuan pengabdian masyarakat adalah membangun desa-desa binaan tanggap COVID-19, lupus, reumatik, dan alergi di Malang oleh Tim Kelompok Kajian Lupus, Autoimun, Reumatik, dan Alergi (LAURA) Universitas Brawijaya. Warga desa binaan diberikan penyuluhan berupa seminar awam dan pelatihan tentang pertolongan awal pada penyakit COVID-19, lupus, reumatik, dan alergi, kemudian diminta mengisi kuesioner posttest untuk mengukur pemahaman. Desa-desa binaan diberikan thermo-gun dan wastafel untuk menerapkan protokol kesehatan. Satu bulan kemudian dievaluasi adanya kejadian COVID-19, lupus, reumatik, dan alergi di desa binaan.Â Hasil evaluasi menunjukkan rata-rata warga desa binaan memahami 78,3% materi yang diberikan dan menerapkan protokol kesehatan sesuai yang diajarkan saat penyuluhan. Dilaporkan tidak ada kejadian COVID-19 serta kekambuhan lupus, reumatik, dan alergi dalam satu bulan terakhir kegiatan. Kesimpulan: pembangunan desa-desa binaan di Malang meningkatkan tanggap warga terhadap COVID-19, lupus, reumatik, dan alergi.

Effect of Propolis on the Adhesion Index, Morphology and Viability of Candida albicans Cells on Biofilm Formation Dinda Oktia Maghfiroh; A.A. Santi Dewi; Anggie Diniayuningrum; Bambang Rahardjo; Nurdiana Nurdiana; Agustina Tri Endharti; Sumarno Sumarno
Medical Laboratory Technology Journal Vol. 7 No. 1 (2021): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Approximately 70-75% of reproductive women have experienced vulvovaginal candidiasis at least once, and 40-45% will have recurrences. Candida albicans is the most etiology of vulvovaginal candidiasis and is able to form a biofilm that can lead to antifungal resistances and recurrences. One of the natural products that have an anti biofilm effect is propolis. This study expected that propolis from Lawang can be one of the anti-biofilm agent candidates for antifungal resistant cases. This study aimed to know the anti-biofilm effect of propolis ethanol extract on cell adhesion index, morphology, and viability of Candida albicans cell on biofilm formation. The adhesion index was counted on 100 epithelial cells under light microscopy (1000x). Morphology was seen using light microscopy (400x). Cell viability was examined by CFU assay. At 12.5% concentration of propolis ethanol extract, adhesion index decreased (p=0,000), and hyphal growth was inhibited. Colony growth decreased at 2.5% concentration and was not seen at 10% concentration of propolis ethanol extract (p=0,000). These results indicated that propolis ethanol extract could decrease adhesion index, failed the Candida albicans morphology transition from yeast to hyphal, and decreased Candida albicans cell viability on biofilm formation. Propolis ethanol extract is likely to be one of the alternatives to recurrent vulvovaginal candidiasis treatment, especially caused by Candida albicans biofilm formation.

Nigella sativa Extract Increases Antibacterial Activity by Up-Regulating T-reg and Th2 Levels in Salmonella enterica subsp. enterica serovars Typhimurium-Exposed Balb/c Mice Zahra Ahmed; Sumarno Reto Prawiro; Agustina Tri Endharti
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Nigella sativa extract (NSE) is known to be an effective antibacterial and immuno-modulating agent. This study aimed to demonstrate that treatment of Salmonella eterica subsp. eterica serovars typhimurium-infected mice with NSE would cause an increase in T-regulatory (T-reg) and Th2 cells and a decrease in the number of spleen bacterial colonies. Mice were randomly divided into five groups [control (T0), mice infected with S. typhimurium only (T1), and mice infected with S. typhimurium and then treated with NSE 0.52, 5.2, or 52 mg/kg body weight NSE (N1, N2, and N3, respectively)]. Levels of T-reg and Th2 cells were determined via flow cytometry and the number of spleen bacterial colonies was determined by observation. The results showed that treatment with NSE 0.52, 5.2, and 52 mg/kg significantly increased the number of T-reg and Th2 cells (p<0.05) relative to T0. Administration of NSE 5.2 mg/kg increased the number of T-reg cells, while administration of NSE 52 mg/kg caused the greatest increase in the number of Th2 cells in the spleens of S. typhimurium-infected mice. Moreover, no S. typhimurium colonies were found in the spleens of any NSE-treated mice. Our results suggest that NSE has therapeutic potential to ameliorate S. typhimurium infection.

Neutrophil, TLR2, and TLR4 expression in newborns at risk of sepsis Ari Yunanto; Agustina Tri Endharti; Aris Widodo
Paediatrica Indonesiana Vol 53 No 3 (2013): May 2013
Publisher : Indonesian Pediatric Society

Background There is increasing evidence that toll-like receptors (TLR) play a key role in the mediation of systemic responses to invading pathogens during sepsis. Saliva is an important body fluid for detecting physiological and pathological conditions of the human body. Neutrophils are participants in the acute response against pathogens in many tissues, and their influx into the oral cavity may occur at any time.Objective To compare mean neutrophils and the expression of TLR2 and TLR4 in saliva and blood of newborns at risk for sepsis to those of healthy newborns.Methods This cross-sectional study was conducted from July to December 2011 in the Division of Neonatology, Department of Child Health, Ulin General Hospital, Lambung Mangkurat University Medical School, Banjarmasin. Case subjects were newborns with sepsis risk factors (30 infants), while 30 healthy infants were in the control group. Saliva and blood specimen examinations were performed in the Biomedical Laboratory of Brawijaya University Medical School, Malang. We used T-test for statistical analyses.Results From saliva specimens, mean neutrophils were significantly higher in the case group than in the control group [14.43 (SD 12.21) % vs. 5.63 (SD 6.78) %, respectively, (P=0.021)]. In addition, mean TLR2 and mean TLR4 saliva levels were significantly higher in the case group than in the control group [TLR2: 64.97 (SD 26.42) % vs. 40.06 (SD 6.23) %, respectively, (P=0.011); TLR4: 1.5 (SD 1.61) % vs. 0.57 (SD 0.53) %, respectively, (P=0.044)]. From blood specimens, mean neutrophils were also significantly higher in the case group than in the control group [1.09 (SD 0.61)% vs. 0.21 (SD 0.09)%, respectively, (P=0.000)]. Similarly, mean blood TLR2 and TLR4 levels were significantly higher in the case group than in the control group [TLR2: 92.51 (SD 5.51) % vs. 81.74 (SD 11.79) %, respectively, (P=0.003); TLR4: 0.71 (SD 1.42) % vs. 0.12 (SD 0.06) %, respectively, (P=0.000)].Conclusion There are significant increases in neutrophils, asÂ well as neutrophil expression of TLR2 and TLR4 in the saliva and blood from newborns with sepsis risk factors compared to those of healthy newborns. [Paediatr Indones. 2013;53:132-7.]

The PENGARUH EKSTRAK TEH HIJAU TERHADAP EKSPRESI RESEPTOR ESTROGEN-α DAN KETEBALAN ENDOMETRIUM PADA TIKUS YANG DIPAPAR MONOSODIUM GLUTAMAT Irma Hamdayani Pasaribu; Sutrisno Sutrisno; Agustina Tri Endharti
Jurnal Ilmiah Bidan (JIB) Vol 3 No 3 (2018)
Publisher : Ikatan Bidan Indonesia (IBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak teh hijau terhadap ekspresi reseptor estrogen-α dan ketebalan endometrium pada tikus yang dipapar MSG. Desain penelitian eksperimental dengan sampel tikus putih betina sebanyak 25 ekor yang terbagi menjadi 5 kelompok. yaitu kontrol negatif (tanpa perlakuan), kontrol positif (tikus dipapar MSG 140mg/200grBB), 3 kelompok perlakuan masing-masing diberikan paparan MSG140mg/200grBB dan diberikan ekstrak teh hijau dengan 3 dosis berbeda yaitu 0,7mg/tikus/hr, 1,4mg/tikus/hr, 1,8mg/tikus/hr. Paparan MSG dan ekstrak teh hijau berbagai dosis diberikan selama 30 hari. Pemeriksaan ekpresi RE-α menggunakan metode Immunohistokima dan ketebalan endometrium dengan pewarnaan Hematosiklin Eosin. Data dianalisis menggunakan uji One Way Anova. Berdasarkan hasil uji perbandingan berganda dengan uji LSD menunjukkan bahwa rerata ekpresi RE-α tertinggi tampak pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 1,4 mg/tikus/hari (45,37±6,46) namun menurun pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 1,8 mg/tikus/hari (43,05±4,54). Nilai rerata ketebalan endometrium tertinggi tampak pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 1,4 mg/tikus/hari (500,17±15,3) namun menurun pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 1,8 mg/tikus/hari (461,77±39,99). Penelitian ini menunjukkan hasil pemberian ekstrak teh hijau berbagai dosis dapat meningkatkan ekspresi reseptor estrogen-α dan ketebalan endometrium pada tikus yang dipapar monosodium glutamat.

Detection of COVID-19 Cases in the Hospital of Universitas Brawijaya Malang Nuning Winaris; Andre William Tulle; Soeyati Poejiani; Sri Andarini; Wening Prastowo; Agustina Tri Endharti; Dwi Yuni Nur Hidayati
Indonesian Archives and Biomedical Sciences Vol 2 No 1 (2022): Indonesian Archives of Biomedical Research (InABR) 2(1): 2022
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55392/indarcbiores.v2i1.29

The global pandemic of COVID-19 was caused by a novel coronavirus identified as SARS-CoV-2. The outbreak started in Wuhan, China and spread rapidly all over the world. Escalation of daily test capacity can accelerate the new suspect of COVID-19 cases finding and rapidly trace the close contacts of confirmed cases. In this study, we reported the total number of confirmed cases based on the number of samples tested in the diagnostic laboratory of the Hospital of Universitas Brawijaya Malang. The data for this study were collected from the result of the RT-qPCR test, which was used as the gold standard to identify active cases of COVID-19. The data collection was started from May 2020 until April 2021. Based on our findings, it appeared that the peak of the first wave of COVID-19 happened between December 2020 and January 2021. Keywords: COVID-19; RT-qPCR; ‎‎‎‎‎‎‎‎‎‎‎‎SARS-CoV-2

Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Samsul Arifin; Loeki Enggar Fitri; Hidayat Sujuti; Bagus Hermansyah; Agustina Tri Endharti; Niniek Burhan; Didi Candradikusuma; Erma Sulistyaningsih; Josef Sem Berth Tuda; Umar Zein
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.

Cosmos caudatus Leaf Ethanol Extract Inhibit Growth and Viability through Membrane Permeability and Apoptosis in Candida albicans Agustina Tri Endharti
Journal of Global Pharma Technology Volume 11 Issue 04.
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.119 KB)

Objective: Candidiasis is the most common fungal gynecological disease in humans. Essentially, the use of antifungal such as Fluconazole has reportedly increased resistance to candidiasis by 7%. The flavonoid has been shown to be used as an antifungal in Candida albicans. Cosmos caudatus contains 51% of Quercetin. To prove the influence of the use of flavonoid antifungal of quercetin group on the growth, viability, membrane permeability, and apoptosis of the C. albicans. Methods: The SV-1148 coded C. albicans isolate was obtained from the Microbiology Laboratory of Brawijaya University, Malang, Indonesia. Initially, the isolate was grown in Sabouraud Dextrose Agar (SDA) medium. The samples were divided into negative and positive control groups of fluconazole (60µg/ml) and difference concentrations (5%, 10%, 20%, and 40%) of leaves ethanol extract of C. caudatus. Results: Concentration of 40% ethanol extract C. caudatus has Minimum Inhibitory Concentration (MIC) of 30%. This result showed that 40% ethanol extract C. caudatus was able to provide the best growth resistance which was characterized by the absence of colonies from C. albicans. The data of Influx Assay Iodide increased membrane permeability of C. albicans to disrupt cell membranes and induce apoptosis. The percentage of apoptosis increased after treatment of 40% ethanol extract C. caudatus in the culture of C. albicans. Conclusion: C. caudatus has antifungal activity and can be used as an herbal drug in C. albicans infection.

A Comparison Study of the Influence of Caffeic Acid Phenethyl Ester and Mitoxantrone in Experimental Autoimmune Encephalomyelitis Balb/C Mice Model: Comparison Effect Cape and Mitoxantrone Alnawajha, Amin; Endharti, Agustina Tri; Santoso, Sunarto; Santosaningsih , Dewi; Satriotomo , Irawan
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.03

A common neurodegenerative condition that still presents clinical challenges is Multiple Sclerosis (MS). Effective multiple sclerosis treatments are sorely needed in clinical settings. Experimental Autoimmune Encephalomyelitis (EAE) is an animal model of multiple sclerosis, a T-cell-mediated disease. Active T-cells differentiate into the Th9 and Th17 subsets, which are controlled by NF-kB and produce the proinflammatory cytokines IL9 and IL17. Because these cytokines are crucial to the pathophysiology of EAE, they have been used as targets for MS therapy. Caffeic acid phenethyl ester (CAPE) is an active ingredient of propolis that has been shown to have immunomodulatory and anti-inflammatory activities. Mitoxantrone is a synthetic antineoplastic agent and cytotoxic immunosuppressive effect used to treat MS. The study aimed to determine whether the two medications have superior efficacy and effect in the treatment of EAE mouse model MS compared to the other. After inducing EAE in mice, CAPE and mitoxantrone were administered to evaluate this therapeutic effectiveness. ELISA was used to measure IL9, IL17 levels and the activity of NF-kBp56. H&E was used to evaluate cell infiltration T lymphocytes for histopathology of spinal cord tissue. Molecular docking was performed to predict the interaction between CAPE and a cytokine. We found that CAPE has a sufficient effect of reducing the level of IL9, IL17, active NF-kBp56, and inflammatory cell infiltration T-lymphocytes in all groups of mice EAE treated with CAPE. In contrast, mitoxantrone reduced cytokines and cell infiltration, so EAE mice treated with both compounds were observed more improvement than other groups. Based on our findings, two medications demonstrated the same efficacy and effect in EAE mice model MS., whereas CAPE did not statistically reach a significant value. While the combination of two medications has the optimal effect.

Chemical Compounds and Antibacterial Activity of Thymus Vulgaris Leaves’ Ethanolic Extract Against Salmonella typhimurium: Thymus vulgaris Against Salmonella typhimurium Abousouh, Emad Khaleefah; Endharti, Agustina Tri; Santoso, Sanarto; Santosaningsih, Dewi
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.09

Invasive non-typhoidal Salmonella is an important pathogen that causes life-threatening bloodstream infections. Antibiotic resistance in non-typhoidal Salmonella has emerged as a result of the increasing use of antibiotics; therefore, an alternative source of therapeutic agents is required. This study aimed to investigate the active compound and antibacterial activity of Thymus vulgaris leaves’ ethanolic extract against Salmonella typhimurium. Thin-layer chromatography was performed to identify the active compound in the ethanolic extract of T. vulgaris leaves. The antibiotic effect of the extract was carried out by agar dilution assay using the following concentrations: 0%, 2.5%, 5.0%, 7.5%, 10.0%, 12.5%, and 15.0% (w/v). Thymol and carvacrol were detected in the thin-layer chromatography. The minimum inhibitory concentration of the extract was 10.0% (w/v). In conclusion, T. vulgaris leaves’ ethanolic extract demonstrated antimicrobial activity against S. typhimurium. Further investigation is required to analyze the role of thymol and carvacrol as active compounds against S. typhimurium.

Co-Authors A.A. Santi Dewi Abd. Rasyid Syamsuri Abousouh, Emad Khaleefah Alnawajha, Amin Ambar Dwi Retnoningrum Andre William Tulle Anggie Diniayuningrum Ari Yunanto Arifin, Mochammad Samsul Aris Widodo Bagus Hermansyah Balafif, Farhad Bambang Rahardjo Burhan, Niniek Daniel Happy Putra desy wulandari Dewi Purnama Sari Dewi Santosaningsih Dian Hasanah Didi Candradikusuma Didi Candradikusuma Dinda Oktia Maghfiroh Dwi Yuni Nur Hidayati Elvira Sari Dewi Erma Sulistyaningsih Erryana Martati Ery Olivianto Febriana, Romadhinniar Handono Kalim Hani Susianti Hidayat Sujuti Hittoh Fattory, Hittoh HMS Chandra Kusuma Indriati Dwi Rahayu Irma Hamdayani Pasaribu Josef Sem Berth Tuda Krisni Subandiyah Kusumawati, Shinta Kusworini Handono Loeki Enggar Fitri Maramy, Neyla Vista Muhammad Anshory, Muhammad Muhammad Rivai Nikmatul Ulya, Nikmatul Niniek Burhan Nuning Winaris Nurdiana Nurdiana Nurdiana Nurdiana Perdana Aditya Rahman Prawiro, Sumarno Reto R Setyohadi, R Rahmad Rahmad Rima Zakiyah Samsul Arifin Sanarto Santoso Santosaningsih , Dewi Santoso, Sunarto Sari, Tita Luthfia Satriotomo , Irawan Soeyati Poejiani Solly Aryza Sri Andarini Subandi Subandi Sumarno . Sumarno Reto Prawiro Sumarno Sumarno Sutrisno Sutrisno Taqiyya, Nadia Tatit Nurseta Tri Dewanti Widyaningsih Tuda, Josef Sem Berth Umar Zein Vanji Budi Himawan, Vanji Budi Wahono, Cesarius Singgih Wahyono, Tikto Wardhani, Ratih Kusuma Wening Prastowo Wisnu Barlianto Zahra Ahmed

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