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All Journal Biotika: Jurnal Ilmiah Biologi Microbiology Indonesia International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Jurnal Kesehatan Andalas Padjadjaran Journal of Dentistry Paediatrica Indonesiana Jurnal Ilmiah Kesehatan Anakes: Jurnal Ilmiah Analis Kesehatan Journal Medical Informatics Technology
Prima Nanda Fauziah
Universitas Mohammad Husni Thamrin
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Padjadjaran Journal of Dentistry

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Optimized steps in determination of malondialdehyde (MDA) standards on diagnostic of lipid peroxidation Fauziah, Prima Nanda; Maskoen, Ani Melani; Yuliati, Tri; Widiarsih, Erlina
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.348 KB) | DOI: 10.24198/pjd.vol30no2.18329

Abstract

Introduction: Lipid peroxidation, one of the known indices of oxidative stress, is documented in various diseases. Secondary oxidation products such as malondialdehyde (MDA) is commonly measured to observe lipid peroxidation. In this study, a spectrophotometric method was evaluated to measure thiobarbituric acid reactive substances (TBARS) with high sensitivity. This study was aimed to optimisation standard of MDA using tetraethoxypropane (TEP) 97% (FW=220.3). Methods: The method is based upon the reaction of malondialdehyde (MDA) and TBA in the glacial acetic acid medium. MDA is a known biomarker of oxidative status in a biological system. This research consists of two phases: first, making a stock of TEP, and the second phase was testing the concentration of TEP for finding the standard curve of MDA before used in diagnostic of lipid peroxidation. Results: Result showed the concentration 1,875-60 uM of TEP could form a precise standard curve. Conclusion: This concentration of TEP can be used as a reference as the standard of control in diagnostic of lipid peroxidation using TBARS method.
Optimized steps in determination of malondialdehyde (MDA) standards on diagnostic of lipid peroxidation Fauziah, Prima Nanda; Maskoen, Ani Melani; Yuliati, Tri; Widiarsih, Erlina
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.348 KB) | DOI: 10.24198/pjd.vol30no2.18329

Abstract

Introduction: Lipid peroxidation, one of the known indices of oxidative stress, is documented in various diseases. Secondary oxidation products such as malondialdehyde (MDA) is commonly measured to observe lipid peroxidation. In this study, a spectrophotometric method was evaluated to measure thiobarbituric acid reactive substances (TBARS) with high sensitivity. This study was aimed to optimisation standard of MDA using tetraethoxypropane (TEP) 97% (FW=220.3). Methods: The method is based upon the reaction of malondialdehyde (MDA) and TBA in the glacial acetic acid medium. MDA is a known biomarker of oxidative status in a biological system. This research consists of two phases: first, making a stock of TEP, and the second phase was testing the concentration of TEP for finding the standard curve of MDA before used in diagnostic of lipid peroxidation. Results: Result showed the concentration 1,875-60 uM of TEP could form a precise standard curve. Conclusion: This concentration of TEP can be used as a reference as the standard of control in diagnostic of lipid peroxidation using TBARS method.
Co-Authors - Chrysanti Aditia, Erie Affiyanti, Wirda Akhmad Yogi Pramatirta, Akhmad Yogi Alislam, Mochamad Arief Ani Melani Maskoen Aruna Gamarani Atna Permana Audia, Desi Basyir, Vaulinne Budi Handono Catu Umirestu Nurdiani Chrysanti Chrysanti Damayanti, Vila Dany Hilmanto Desi Audia Despiagia Pitaloka Dian Firmansyah Dida A. Gurnida DIKI HILMI Dzulfikar Djalil Lukmanul Hakim El Jannah, Syarifah Miftahul Ellis Susanti Erie Aditia Esti Istiqomah Fadil Oenzil Fakhrizal Hariyanto Wahdi Harsono Setiawan Heru Purwanto Nugroho IIS HERAWATI Iis Herawati Imas Latifah Imas Latifah Januar Rhamadani Jeffry Iman Gurnadi Jetty Nurhajati Jetty Nurhajati Johanes C. Mose Johanes C. Mose Latifah, Imas Lenggo Geni Lestari, Estu Mahmudah Mahmudah Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto Masdianto, Masdianto Mochamad Arief Alislam Mukti, Septiana Widya Murleni Dorawati Nugroho, Heru Purwanto Nurdiani, Catu Umirestu Permana, Atna Pitaloka, Despiagia Pratiwi, Isnaini Nisa Restu Rano Agustino Ratna Mutu Manikam Retno Martini Widhyasih Septiana Widya Mukti Subastiono, Aditia Suhara, Intan Ryatni Syahrial Harun Syarifah Miftahul El Jannah Vaulinne Basyir Vira Amalia Wahdi, Fakhrizal Hariyanto Widhyasih, Retno Martini Widiarsih, Erlina Widiarsih, Erlina Wirda Affiyanti Yanwirasti Yanwirasti Yanwirasti Yanwirasti Yuliati, Tri Yuliati, Tri Yunia Qonitatin Al Masyani Zaranggi, Ahmad ZURAIDA ZURAIDA Zuraida Zuraida