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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Buletin Peternakan Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) ARSHI Veterinary Letters Jurnal Kedokteran Hewan
Mokhamad Fahrudin
Departemen Anatomi Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln Agathis, Dramaga, Bogor, Jawa Barat, Indonesia, 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : Hemera Zoa

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PCS-1 Development of Mouse Parthenogenetic Embryos in Phosphate Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution so that embryos will develop without fertilization [3]. The process of parthenogenesis as a reproductive strategy occurs in species of insect, pisces, or amphibian, which not require any implantation. Naturally, parthenogenesis is not common in mammals, but by understanding cellular mechanism during fertilization, it is possible to artificially activate mammalian oocytes.Blastocyst, derived from parthenogenesis, can be used for developmental study, embryo reconstruction, and one of potential source for pluripotent stem cells. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate.One of the component that has been predicted to inhibit parthenogenetic embryo development is phosphate. Haraguchi et al. (1996)    reported that phosphate caused a negative effect on in vitro culture of AKR/N mice fertilized embryos, removal of phosphate elements was significantly improved the blastocyst rate up to 42.6% [1]. The effects of phosphate also became an interesting finding in the study that reported mouse fertilized embryos could well developed in modified medium rat 1 cell embryo medium (MR1ECM) which not contained any phosphate [2].The effect of phosphate on in vitro culture of mouse parthenogenetic embryo has not been clear. The aim of this research was to analyze inhibitory effect caused by phosphate in the medium and compare the development pattern between parthenogenetic and fertilized embryos in order to reach optimal production of parthenogenetic blastocyst for further purposes.  
PF-17 The Development of Crude Testicular Cells in In Vitro Culture Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Spermatogenesis is a continuous process in which spermatogonial stem cells (SSC) develop into specific germ cells before terminally differentiating to form spermatozoa.  The process is supported by Sertoli cells, which are in close contact with germ cells in the seminiferous tubules. Sertoli cells provide essential hormonal signals, nutrients, and physical support to germ cells for successful spermatogenesis.The crude testicular cells (CTC) contains many cell types, like Sertoli cell, Leydig cell, spermatogonial stem cell (SSC), spermatocyte and other testicular somatic cells (Shah et all. 2016). Testicular cells are believed to secrete various growth factors that induced the spermatogenesis process.  The spermatogonial stem cells are unique population of cells in the male testis, which dual function.  First self-renewing their population to maintain the number of stem cells, secondary function is differentiating into spermatids in testis (Wang et al.  2015).Spermatogenic cells differentiation  needed the similar microenvironment in vivo spermatogenesis.  The essential nutrients was collected from healty culture and the culture contained mixed population of cells both the somatic cells and spermatogenic cells.  To identification the spermatogenic cells using Periodic Acid Schifft (PAS) staining (Chang et al. 2011). The present study examined the development of crude testicular cells using PAS staining.
Co-Authors Aisyah Fidela Siregar Al Mukhlas Fikri Alfiah, Elma Alkaustariyah Lubis Almanda, Wisnu Rahmadan Arief Boediono Berry Juliandi Boenjamin Setiawan Budiariati, Vista Cece Sumantri Diah Nugrahani Pristihadi Dwi Budiono El Zamzami, Arfah Herawana Elmanaviean, Muhammad Gusdinar, Rizal Hardinsyah Harry Murti Ita Djuwita Katrin Roosita Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Kombo, Muhammad Piter Kusdiantoro Mohamad M Agus Setiadi M. Haviz Makhroja, Lalu Faris Naufal Maria Ulfah Muhamad Rizal Martua Damanik Muhammad Gunawan Muhammad Gunawan Muhammad Rosyid Ridlo Ni Wayan Kurniani Karja Noer Muhammad Dliyaul Haq Nugraha, Arifin Budiman Nurwati, Yuni Nur’aisyah Amrah Safitri Pattikawa, Vapriel Andhika Peni Soeprapti Hardjosworo R. Iis Arifiantini Rahmaniyah, Wiwit Ridhani Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Rini Widyastuti Rukmiasih Rukmiasih Safika S, Safika Sri Anna Marliyati Srihadi Agungpriyono Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Triyaningrum, Triyaningrum Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wiwit Ridhani Rahmaniyah Yusuf Kurniawan Zulfikar, Muhammad Irsyad