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All Journal Pendidikan Ekonomi Medical Laboratory Technology Journal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Poltekita : Jurnal Ilmu Kesehatan Jaringan Laboratorium Medis Borneo Journal of Medical Laboratory Technology Journal SAGO Gizi dan Kesehatan Poltekita: Jurnal Pengabdian Masyarakat Jurnal Ilmiah Pengabdian Masyarakat Bidang Kesehatan Journal of Development Education and Learning
Dina Afrianti1
Program Studi Teknologi Bank Darah, Jurusan Analis Kesehatan, Politeknik Kesehatan Kemenkes Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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Modifikasi Larutan Fiksasi Menggunakan Gliserol Bertingkat pada Pembuatan Preparat Awetan Telur Soil Transmitted Helminths : Modification of Fixative Solution Using Gradual Glycerol in the Preparation of Preserved Slides of Soil Transmitted Helminths Egg Mufti, Meyda Fianisa; Afrianti, Dina
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 1 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i1.7434

Abstract

Preparat awetan merupakan bentuk media pemeriksaan dan pembelajaran mikroskopis yang dapat digunakan secara berulang dengan waktu pemakaian yang lama. Kesulitan dalam pembuatan preparat awetan telur Soil Transmitted Helminths (STH) menyebabkan terbatasnya pengadaan preparat awetan. Preparat awetan yang dibuat menggunakan metode wet mount tidak bertahan lama karena dapat mengering dalam waktu yang singkat yaitu kurang dari satu bulan. Penelitian dilakukan untuk mengetahui pengaruh pemberian larutan fiksasi gliserol terhadap ketahanan preparat awetan telur STH selama 12 minggu. Penelitian ini merupakan penelitian eksperimental dengan 4 perlakuan dan 6 pengulangan, dengan total sampel sebanyak 27. Sampel yang digunakan adalah suspensi sampel feses positif STH. Analisis data yang digunakan adalah Kruskal Wallis dan Man Whitney U. Hasil uji Kruskal Wallis menunjukkan adanya perbedaan yang bermakna antara preparat tanpa gliserol dan dengan gliserol 30%, 50%, dan 70% (p = 0.002). Dapat disimpulkan bahwa ada perbedaan yang bermakna antara preparat tanpa gliserol dan dengan gliserol 30%, 50%, dan 70% (p = 0.002) dan didapatkan hasil pada konsentrasi gliserol 50% lebih optimal dalam mempertahankan preparat hingga bulan ketiga.
The Effects of Sungkai Leaf and Cinnamon Bark Extracts on MDA and IL-10 in MSG Induced Chronic Kidney Disease in Wistar Rats Afrianti, Dina; Maulana, Mochamad Rizal; Sakdiah, Siti; Febriyani, Erisa; Wahyudi, Wahyudi; Ali, Irwan
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.661

Abstract

Monosodium glutamate (MSG) is known to trigger oxidative stress and inflammation, contributing to kidney tissue damage. This study aimed to evaluate the protective effects of sungkai leaf extract (Peronema canescens Jack) and cinnamon bark extract (Cinnamomum burmannii) on kidney histology, as well as malondialdehyde (MDA) and interleukin-10 (IL-10) levels in male Wistar rats induced with excessive MSG. A total of 30 rats were divided into six groups: normal control, positive control (MSG only), and four treatment groups receiving MSG alongside sungkai leaf extract (28 mg or 56 mg) or cinnamon bark extract (4% or 8%) for 10 days. MDA and IL-10 levels were measured using ELISA, and kidney tissue was examined histologically. The results showed that both extracts significantly reduced MDA (P= 0,000)  levels and increased IL-10 (P= 0,000)   levels compared to the positive control. The most prominent protective effect was observed in the group treated with 56 mg of sungkai leaf extract, followed by the 8% cinnamon bark group. Histopathological analysis revealed notable improvements in kidney structure, approaching normal conditions in treated groups. These findings suggest that sungkai leaf and cinnamon bark extracts, particularly at higher doses, offer protective benefits against MSG-induced kidney damage through antioxidant and anti-inflammatory mechanisms.
Co-Authors Adita Puspitasari Swastya Putri Adita Puspitasari Swastya Putri Ali, Irwan Aminahtun Latifah Anggraini, Maysita Ari CHODIJAH, CHODIJAH Citra Ramayan Desi Areva Dewi Kurniasih Dewi Kurniasih Djoko Priyatno, Djoko Duri, Iin Desmiany Eko Naning Sofyanita Elis Susilawati Febriyani, Erisa Kuncara, Rachmad Bayu Latifah, Aminahtun Maulana, Mochamad Rizal Mufti, Meyda Fianisa Muslina Muslina Muslina Muslina, Muslina Negoro, Muhamad Syamsul Arif Setiyo Nurul Qomariyah Putri, Adita Puspitasari Swastya Rachmad Bayu Kuncara Rahmadhani, Afiffah Gita Ririh Jatmi Wikandari Rodhi Hartono Roni Afriansya Sakdiah, Siti Setyowatiningsih, Lilik Sofyanita, Eko Naning Surati Surati Susilawati, Elis Swastya Putri, Adita Puspitasari Teguh Budiharjo, Teguh Titiek Sumarawati WAHYUDI Wahyudi Wahyudi Wahyudi Wahyudi Widodo Widodo